##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR491082_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 37418616 Sequences flagged as poor quality 0 Sequence length 100 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.67003921256735 31.0 31.0 34.0 27.0 34.0 2 30.99807432749517 31.0 31.0 34.0 27.0 34.0 3 31.101411767875113 33.0 31.0 34.0 27.0 34.0 4 34.53424993056932 37.0 35.0 37.0 31.0 37.0 5 34.226268684015466 37.0 35.0 37.0 30.0 37.0 6 34.14002671825168 37.0 35.0 37.0 30.0 37.0 7 34.08971154892527 37.0 35.0 37.0 30.0 37.0 8 34.04978847961667 37.0 35.0 37.0 30.0 37.0 9 35.53599587969796 39.0 35.0 39.0 30.0 39.0 10-11 35.424055983257105 39.0 35.0 39.0 29.5 39.0 12-13 35.413560151449744 39.0 35.0 39.0 30.0 39.0 14-15 36.570386983313334 40.0 36.0 41.0 30.0 41.0 16-17 36.501125629552945 40.0 36.0 41.0 29.0 41.0 18-19 36.46327507142434 40.0 36.0 41.0 29.0 41.0 20-21 36.43746181045285 40.0 36.0 41.0 29.0 41.0 22-23 36.32369953233973 40.0 36.0 41.0 28.0 41.0 24-25 36.203541360268375 40.0 36.0 41.0 27.5 41.0 26-27 36.06715086683056 40.0 36.0 41.0 27.5 41.0 28-29 35.83948893246078 39.5 35.0 41.0 27.0 41.0 30-31 35.63664682306796 39.0 35.0 41.0 27.0 41.0 32-33 35.436627319407 39.0 35.0 41.0 26.0 41.0 34-35 35.27159369015679 39.0 35.0 41.0 25.0 41.0 36-37 34.99698633963372 39.0 35.0 41.0 24.0 41.0 38-39 34.86638915239409 39.0 34.5 41.0 24.0 41.0 40-41 34.64249666529622 39.0 34.0 41.0 23.5 41.0 42-43 34.30320868895846 38.0 34.0 40.0 21.5 41.0 44-45 33.928917667612296 38.0 33.0 40.0 17.5 41.0 46-47 33.771594411722766 38.0 33.0 40.0 15.0 41.0 48-49 33.54647544954629 38.0 33.0 40.0 11.0 41.0 50-51 33.322564094834505 38.0 33.0 40.0 8.5 41.0 52-53 33.10952774148568 38.0 33.0 40.0 7.5 41.0 54-55 32.88239479514689 38.0 32.0 40.0 6.5 41.0 56-57 32.59008318212518 37.0 32.0 40.0 2.0 41.0 58-59 32.27211828198028 37.0 31.5 40.0 2.0 41.0 60-61 31.915781906524817 36.0 31.0 40.0 2.0 41.0 62-63 31.700512413393376 36.0 31.0 39.5 2.0 41.0 64-65 31.643246278804114 36.0 31.0 39.0 2.0 41.0 66-67 31.387800794128786 35.0 31.0 39.0 2.0 41.0 68-69 31.032601913977793 35.0 31.0 39.0 2.0 41.0 70-71 30.616775470797744 35.0 30.0 38.0 2.0 40.0 72-73 30.271635100026145 35.0 30.0 37.0 2.0 39.5 74-75 29.920072564950026 35.0 30.0 37.0 2.0 39.0 76-77 29.139186387866403 34.0 29.0 36.0 2.0 39.0 78-79 29.256212522665187 34.5 29.0 36.0 2.0 38.5 80-81 29.044620650854643 34.5 29.0 35.0 2.0 37.0 82-83 28.74590897482686 34.0 29.0 35.0 2.0 37.0 84-85 28.38891946190634 34.0 29.0 35.0 2.0 36.0 86-87 28.131323977883095 34.0 29.0 35.0 2.0 36.0 88-89 27.93356345675639 34.0 29.0 35.0 2.0 36.0 90-91 27.7100080478658 34.0 27.5 35.0 2.0 35.5 92-93 27.460832958119028 34.0 27.0 35.0 2.0 35.0 94-95 27.139692539670627 34.0 26.5 35.0 2.0 35.0 96-97 26.839516472228688 34.0 25.5 35.0 2.0 35.0 98-99 26.535156992444616 34.0 25.0 35.0 2.0 35.0 100 25.826586076834054 33.0 23.0 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.5416048370029145 1101 2 0.4996638443964798 1101 3 0.5598326100634168 1101 4 0.5795520751169079 1101 5 0.6609155394621666 1101 6 0.6611654221074588 1101 7 0.655242304859712 1101 8 0.5827636574789139 1101 9 0.8531376232460559 1101 10-11 0.8963094443011457 1101 12-13 0.8368822227049222 1101 14-15 1.002201516169869 1101 16-17 0.931331900728857 1101 18-19 0.9588205004447659 1101 20-21 0.9021954774669041 1101 22-23 0.9184187603022949 1101 24-25 0.936509580081804 1101 26-27 0.8873580538764969 1101 28-29 0.8397842230195565 1101 30-31 0.9301417612410106 1101 32-33 0.9738303784513818 1101 34-35 0.8780421037186059 1101 36-37 1.0224492260945084 1101 38-39 0.9109309490619566 1101 40-41 0.8931564767896347 1101 42-43 0.8554475540635309 1101 44-45 0.839255499172701 1101 46-47 0.7493513309188486 1101 48-49 0.8183964618997379 1101 50-51 0.7623666271606382 1101 52-53 0.6050491356568344 1101 54-55 0.6198229100478514 1101 56-57 0.6732450744693566 1101 58-59 0.4938119114717381 1101 60-61 0.6772910674349788 1101 62-63 0.7696460892624408 1101 64-65 0.809294910426253 1101 66-67 0.8171589622444237 1101 68-69 0.8128079622365867 1101 70-71 0.795442691973296 1101 72-73 0.7576802693242861 1101 74-75 0.6198923648986678 1101 76-77 0.5566319745173267 1101 78-79 0.6797902072897806 1101 80-81 0.680454293869662 1101 82-83 0.7718460805635523 1101 84-85 0.7756010558635396 1101 86-87 0.7048591606005097 1101 88-89 0.7174129984954156 1101 90-91 0.7425708686590404 1101 92-93 0.7043158690746161 1101 94-95 0.774805979130921 1101 96-97 0.6710560367283485 1101 98-99 0.5465082371071546 1101 100 0.5512638528901732 1102 1 0.4484190897099296 1102 2 0.4012368476242365 1102 3 0.39297962891534866 1102 4 0.40441112681018154 1102 5 0.5490986897085008 1102 6 0.5308964566506447 1102 7 0.46952972358563727 1102 8 0.5481598385197657 1102 9 0.6357964021193752 1102 10-11 0.7021856011813838 1102 12-13 0.7182591923772534 1102 14-15 0.7094719633260027 1102 16-17 0.7989516472368408 1102 18-19 0.7431837948251427 1102 20-21 0.7358192398824528 1102 22-23 0.7402346760364154 1102 24-25 0.76727836920778 1102 26-27 0.7606476378575451 1102 28-29 0.7867032933233986 1102 30-31 0.766050486619541 1102 32-33 0.8084003961022574 1102 34-35 0.7942803997246912 1102 36-37 0.9063861776874376 1102 38-39 0.8438295560209141 1102 40-41 0.7842050254759059 1102 42-43 0.8002735991694507 1102 44-45 0.8253847561832472 1102 46-47 0.7458650353711107 1102 48-49 0.8660307160004237 1102 50-51 0.7558662703918699 1102 52-53 0.6887008022913292 1102 54-55 0.7682006389148128 1102 56-57 0.6938441961555668 1102 58-59 0.7113282193366288 1102 60-61 0.7739004002239795 1102 62-63 0.7103081612648445 1102 64-65 0.7542546514115784 1102 66-67 0.7266053532189041 1102 68-69 0.7796428995919094 1102 70-71 0.7863114804010642 1102 72-73 0.7318819410173383 1102 74-75 0.739722137184085 1102 76-77 0.7317459153186299 1102 78-79 0.8003428915366833 1102 80-81 0.8101421752723823 1102 82-83 0.7804731149566884 1102 84-85 0.7884031490667098 1102 86-87 0.7510815084538898 1102 88-89 0.7867962707632437 1102 90-91 0.710007546993932 1102 92-93 0.656466283938105 1102 94-95 0.7287243690961631 1102 96-97 0.7131601199099578 1102 98-99 0.7792803150497676 1102 100 0.7776957127246753 1103 1 0.15808847200669618 1103 2 0.13299213579585967 1103 3 0.16628165783055593 1103 4 0.18382741682496828 1103 5 0.14839149002320084 1103 6 0.18686102104433644 1103 7 0.24741592086913755 1103 8 0.26969479040940314 1103 9 0.14011448346512623 1103 10-11 0.24426050830717827 1103 12-13 0.27282800446039346 1103 14-15 0.208958322728499 1103 16-17 0.2319731954275639 1103 18-19 0.2090395801396383 1103 20-21 0.2606296788237188 1103 22-23 0.34476240824628945 1103 24-25 0.327584920735994 1103 26-27 0.2630596057753536 1103 28-29 0.2983946637900914 1103 30-31 0.3508078099424381 1103 32-33 0.272249276463441 1103 34-35 0.1910194703489978 1103 36-37 0.3737033261752458 1103 38-39 0.33060427717175855 1103 40-41 0.2113786334585228 1103 42-43 0.13803146606484518 1103 44-45 0.20877272049926177 1103 46-47 0.23687086653388434 1103 48-49 0.2058826678046657 1103 50-51 0.18087389338921156 1103 52-53 0.1751019125255695 1103 54-55 0.2546870236043901 1103 56-57 0.2913915224218897 1103 58-59 0.22918287036027607 1103 60-61 0.27613866930906994 1103 62-63 0.28928225830192034 1103 64-65 0.2441628951993664 1103 66-67 0.13133259820807197 1103 68-69 0.24310874318879883 1103 70-71 0.3377062195010829 1103 72-73 0.2988577046628187 1103 74-75 0.25607357512116735 1103 76-77 0.18910138322914705 1103 78-79 0.2803978217652805 1103 80-81 0.341103750861123 1103 82-83 0.29233267438405264 1103 84-85 0.3890106362127881 1103 86-87 0.36888026746300895 1103 88-89 0.2537331297821801 1103 90-91 0.1917121075348973 1103 92-93 0.23886682842215734 1103 94-95 0.3345427647497843 1103 96-97 0.408243745390088 1103 98-99 0.4363892565482672 1103 100 0.4138378769112627 1104 1 -0.13611077686641693 1104 2 -0.18130461620521388 1104 3 -0.12618770997631046 1104 4 -0.1181718233762723 1104 5 -0.08490436716140692 1104 6 -0.3158029785928491 1104 7 -0.11219516721187972 1104 8 -0.12866630921021738 1104 9 -0.15304768910684174 1104 10-11 -0.04092910267808492 1104 12-13 -0.11704830170927494 1104 14-15 -0.19778073848268463 1104 16-17 -0.32570130496262806 1104 18-19 -0.20681555793346007 1104 20-21 -0.16805931742258906 1104 22-23 -0.20956333883048472 1104 24-25 -0.22075350961026885 1104 26-27 -0.21556372775991406 1104 28-29 -0.16272567816474748 1104 30-31 -0.12287242461493975 1104 32-33 -0.1637641310993132 1104 34-35 -0.2504446541339007 1104 36-37 0.06487659182158723 1104 38-39 -0.0986271266575045 1104 40-41 -0.19902309810867536 1104 42-43 -0.159977868187255 1104 44-45 -0.1761690332563859 1104 46-47 -0.20061957310681322 1104 48-49 -0.18956864275837404 1104 50-51 -0.26705995714570463 1104 52-53 -0.20179873386816638 1104 54-55 -0.15481804012090095 1104 56-57 -0.23447433605087298 1104 58-59 -0.302716865624717 1104 60-61 -0.22631356320908225 1104 62-63 -0.25817273004137675 1104 64-65 -0.29250614297634314 1104 66-67 -0.17222533606522816 1104 68-69 -0.23373031474989503 1104 70-71 -0.14490006376503217 1104 72-73 -0.12583790933004835 1104 74-75 -0.06255734999549745 1104 76-77 -0.20234743502774322 1104 78-79 -0.18950651499848092 1104 80-81 -0.22300322091424718 1104 82-83 -0.16442359801014916 1104 84-85 -0.17054568451575847 1104 86-87 -0.11962258727436748 1104 88-89 -0.1608363604630192 1104 90-91 -0.2767637181746352 1104 92-93 -0.15436723567320243 1104 94-95 -0.10864735033267081 1104 96-97 -0.08795475607114156 1104 98-99 -0.14616296119963934 1104 100 -0.10828955083542624 1105 1 -0.4081973513313564 1105 2 -0.41834817711404426 1105 3 -0.39560703395342145 1105 4 -0.3466334734679535 1105 5 -0.39478542121423743 1105 6 -0.34670458832950146 1105 7 -0.48415489549471147 1105 8 -0.4002955554321588 1105 9 -0.5443147003265523 1105 10-11 -0.46518496180795665 1105 12-13 -0.5857679663634698 1105 14-15 -0.7292673525737214 1105 16-17 -0.6167090757294602 1105 18-19 -0.6101767915392529 1105 20-21 -0.5984515945160638 1105 22-23 -0.5326008502661352 1105 24-25 -0.5058306052578061 1105 26-27 -0.576510271654854 1105 28-29 -0.5157449538638375 1105 30-31 -0.5240612076050795 1105 32-33 -0.48769972685711593 1105 34-35 -0.4869739526310184 1105 36-37 -0.3206211399579644 1105 38-39 -0.47763154680898623 1105 40-41 -0.420318562079558 1105 42-43 -0.543312041330779 1105 44-45 -0.4158450240097764 1105 46-47 -0.4396201260347894 1105 48-49 -0.40446326808248756 1105 50-51 -0.48040831804370754 1105 52-53 -0.4830240629968827 1105 54-55 -0.4398012932159361 1105 56-57 -0.4412108148280609 1105 58-59 -0.4031953871097613 1105 60-61 -0.3264786461449809 1105 62-63 -0.38105194781627816 1105 64-65 -0.41766397930533117 1105 66-67 -0.42673846539800664 1105 68-69 -0.4479049592266975 1105 70-71 -0.3036294583603514 1105 72-73 -0.3466389707620898 1105 74-75 -0.3738042112797686 1105 76-77 -0.4735643025885814 1105 78-79 -0.4531761978846909 1105 80-81 -0.3723597500617366 1105 82-83 -0.3209508232676903 1105 84-85 -0.2667120241677772 1105 86-87 -0.26411154712784324 1105 88-89 -0.33601990671681037 1105 90-91 -0.3428110423641755 1105 92-93 -0.29407509089973516 1105 94-95 -0.26578678289326163 1105 96-97 -0.29934324195565054 1105 98-99 -0.39460735352656684 1105 100 -0.38958935241143067 1106 1 -0.6738142063504853 1106 2 -0.6099155492250823 1106 3 -0.6429902811126098 1106 4 -0.5283185087100648 1106 5 -0.6668404605152531 1106 6 -0.7043161108824023 1106 7 -0.6734588391073331 1106 8 -0.7183443861302123 1106 9 -0.9884869044746907 1106 10-11 -0.8511771800728809 1106 12-13 -0.8803916321413894 1106 14-15 -1.0324303407268687 1106 16-17 -1.0313134998059823 1106 18-19 -0.9513724876680882 1106 20-21 -0.9717103527314919 1106 22-23 -0.9828992278889714 1106 24-25 -1.024021761131685 1106 26-27 -1.0500424744782464 1106 28-29 -1.2346310540185286 1106 30-31 -1.1452973808370288 1106 32-33 -1.1082929905398373 1106 34-35 -1.0508101858151093 1106 36-37 -0.8927239988997613 1106 38-39 -1.0661125127572646 1106 40-41 -0.9980881966405946 1106 42-43 -0.9860393490412491 1106 44-45 -0.992773155205974 1106 46-47 -0.9316372662123271 1106 48-49 -1.0097822657790587 1106 50-51 -0.9339407052773296 1106 52-53 -1.005977697562436 1106 54-55 -0.9276651152984527 1106 56-57 -0.9523486607171918 1106 58-59 -1.0164107035454677 1106 60-61 -0.9941802258067156 1106 62-63 -0.9915296521498611 1106 64-65 -0.943897194446766 1106 66-67 -0.9699229599229966 1106 68-69 -0.9072170074804546 1106 70-71 -0.9057812010651887 1106 72-73 -0.9350683818217007 1106 74-75 -0.8698419741654817 1106 76-77 -0.9405786530910696 1106 78-79 -0.9234920982422032 1106 80-81 -0.8664109208878905 1106 82-83 -0.8289958462709883 1106 84-85 -0.7586102714991441 1106 86-87 -0.8124172687937339 1106 88-89 -0.8232607421402847 1106 90-91 -0.8599956053843947 1106 92-93 -0.915167828731061 1106 94-95 -0.8009821278899771 1106 96-97 -0.8238636954015739 1106 98-99 -0.8292681864322766 1106 100 -0.78683659703103 1107 1 -1.177805509522745 1107 2 -1.1939077253436352 1107 3 -1.1146909467499668 1107 4 -1.056982878511512 1107 5 -1.1954847539865838 1107 6 -1.2877414409804615 1107 7 -1.3385878833270013 1107 8 -1.2673849642753865 1107 9 -1.5934655811581422 1107 10-11 -1.5287230723589857 1107 12-13 -1.4794998939109476 1107 14-15 -1.8496134903002073 1107 16-17 -1.8754658106399873 1107 18-19 -1.7929906723141826 1107 20-21 -1.7727238564492325 1107 22-23 -1.824701970219813 1107 24-25 -1.850808006828565 1107 26-27 -1.8618624603277851 1107 28-29 -1.8978437824012033 1107 30-31 -1.9792997383180548 1107 32-33 -1.907216291466824 1107 34-35 -1.9989018616688625 1107 36-37 -1.9263992200362807 1107 38-39 -1.9659450990437861 1107 40-41 -2.046577480561993 1107 42-43 -1.9963515967338594 1107 44-45 -1.9286275686195182 1107 46-47 -1.9895435598936864 1107 48-49 -2.124416645701622 1107 50-51 -2.1721192223639463 1107 52-53 -2.089961290337861 1107 54-55 -2.0512156441413723 1107 56-57 -2.05852085846222 1107 58-59 -2.0945025015553824 1107 60-61 -2.106049484450658 1107 62-63 -1.968182026200605 1107 64-65 -2.0884441093908563 1107 66-67 -2.134272772584385 1107 68-69 -2.072688264022112 1107 70-71 -1.9923497513899129 1107 72-73 -2.0565049716410932 1107 74-75 -2.2514027054068038 1107 76-77 -2.413694880791784 1107 78-79 -2.257382923537339 1107 80-81 -2.1847249672302986 1107 82-83 -2.4269660603009555 1107 84-85 -2.226152145862496 1107 86-87 -2.483431548593618 1107 88-89 -2.535563236048855 1107 90-91 -2.859820049742442 1107 92-93 -2.630689878642965 1107 94-95 -2.340114224031691 1107 96-97 -2.3348598370575964 1107 98-99 -2.50172189537194 1107 100 -2.43842596368971 1108 1 -1.8557938675086127 1108 2 -1.84444408258949 1108 3 -1.8064105772839198 1108 4 -1.8089057043571302 1108 5 -1.9606583852239297 1108 6 -2.1138150304112244 1108 7 -2.1721430242639386 1108 8 -2.334428588352239 1108 9 -2.541896030997826 1108 10-11 -2.5217596722120277 1108 12-13 -2.56218075337663 1108 14-15 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1.0525218946185255 2305 98-99 1.038769768906679 2305 100 1.0582396987089773 2306 1 -0.10981307807844232 2306 2 -0.03428161480777803 2306 3 -0.05444559970574403 2306 4 -0.2274146723149144 2306 5 -0.13816455208982603 2306 6 -0.037579256335391165 2306 7 -0.11795740335730187 2306 8 -0.01831260641012733 2306 9 -0.048445500581408396 2306 10-11 0.062394663279079055 2306 12-13 -0.021409602745258383 2306 14-15 -0.015258402093238033 2306 16-17 0.04627150131590696 2306 18-19 0.026413558413317162 2306 20-21 -0.01883610470445518 2306 22-23 -0.020329259184059367 2306 24-25 -0.006686875260143665 2306 26-27 -0.034831744982469104 2306 28-29 -0.010823901615403031 2306 30-31 0.01146810362750017 2306 32-33 0.05280550325204558 2306 34-35 0.08777121071157268 2306 36-37 0.21749518602515394 2306 38-39 0.14879896537281923 2306 40-41 0.1495858374252208 2306 42-43 0.13446539288045756 2306 44-45 -0.020583973142272782 2306 46-47 -0.11990682279843412 2306 48-49 0.08754802358828329 2306 50-51 0.07552709881357345 2306 52-53 0.020146485603042663 2306 54-55 0.10480162565502837 2306 56-57 0.13303756751120943 2306 58-59 0.08011178851118217 2306 60-61 0.20557303438842212 2306 62-63 0.26978542434366304 2306 64-65 0.16171400292604687 2306 66-67 0.1649562702420262 2306 68-69 0.2322889500960308 2306 70-71 0.15524050227606878 2306 72-73 0.13307796051245546 2306 74-75 0.15137031493959796 2306 76-77 0.14458810075712236 2306 78-79 0.1944394593475316 2306 80-81 0.18115081055726634 2306 82-83 0.17130647642528274 2306 84-85 0.33805231462386587 2306 86-87 0.3105817370552195 2306 88-89 0.28682185408807825 2306 90-91 0.2966509135261184 2306 92-93 0.3141230960375232 2306 94-95 0.3478456140695023 2306 96-97 0.39342053722942794 2306 98-99 0.410715291907529 2306 100 0.43100574968744354 2307 1 -0.26964757093590563 2307 2 -0.3065567991439977 2307 3 -0.3201748608351629 2307 4 -0.30012518358071105 2307 5 -0.44707178795546554 2307 6 -0.4389653225583743 2307 7 -0.4581439179208928 2307 8 -0.4191830426201335 2307 9 -0.4693538985739778 2307 10-11 -0.4728084231059313 2307 12-13 -0.4885010728384387 2307 14-15 -0.5919930500906858 2307 16-17 -0.6318984326941575 2307 18-19 -0.626728809399907 2307 20-21 -0.5752981187395321 2307 22-23 -0.699417844545664 2307 24-25 -0.6721944083936862 2307 26-27 -0.5743428046369417 2307 28-29 -0.5994780380745368 2307 30-31 -0.6294815598071821 2307 32-33 -0.6450670497463307 2307 34-35 -0.6161568718140416 2307 36-37 -0.5225106409538114 2307 38-39 -0.6111551871312528 2307 40-41 -0.6936243038852297 2307 42-43 -0.7297999691892088 2307 44-45 -0.6542794640411742 2307 46-47 -0.5152935642433434 2307 48-49 -0.5978130900236422 2307 50-51 -0.6949281033874826 2307 52-53 -0.6144824395802289 2307 54-55 -0.6953312054676672 2307 56-57 -0.6516993796237642 2307 58-59 -0.5591002702175487 2307 60-61 -0.6150365962866644 2307 62-63 -0.6985297309807628 2307 64-65 -0.5489728505990854 2307 66-67 -0.6040944295192148 2307 68-69 -0.6922446935206992 2307 70-71 -1.152973520650498 2307 72-73 -0.7254245548641087 2307 74-75 -0.6069981291318101 2307 76-77 -0.5994365687459471 2307 78-79 -0.6506037670725924 2307 80-81 -0.6579051936761324 2307 82-83 -0.8820036588898823 2307 84-85 -0.7304888371938709 2307 86-87 -0.8160241912085944 2307 88-89 -0.817625243556531 2307 90-91 -0.8114834978365195 2307 92-93 -0.8360023594840627 2307 94-95 -0.9844207121586628 2307 96-97 -0.8174819359821406 2307 98-99 -0.8159710449248614 2307 100 -0.7701651745341067 2308 1 -1.1023502563213512 2308 2 -1.1065214575926525 2308 3 -1.1253139298898844 2308 4 -1.0672611919893598 2308 5 -1.3287417933465662 2308 6 -1.679320241535173 2308 7 -1.4823441335187368 2308 8 -1.486716193375834 2308 9 -1.5827467903215577 2308 10-11 -1.5628063467467612 2308 12-13 -1.539619038726947 2308 14-15 -1.7673196942488048 2308 16-17 -1.7601513071727268 2308 18-19 -1.882427890107472 2308 20-21 -1.9595317346869479 2308 22-23 -1.9853938247318155 2308 24-25 -1.8930417426159707 2308 26-27 -1.79981047360328 2308 28-29 -2.1094683988233314 2308 30-31 -1.9015498617641953 2308 32-33 -1.9263083686246887 2308 34-35 -2.0529748756152415 2308 36-37 -1.908524855302673 2308 38-39 -2.135661139862087 2308 40-41 -1.9286263317486032 2308 42-43 -1.8486720693255734 2308 44-45 -2.0249461153149184 2308 46-47 -2.0251318450343945 2308 48-49 -1.7355048707685796 2308 50-51 -1.6567115151963634 2308 52-53 -1.723581515127556 2308 54-55 -1.830037295713808 2308 56-57 -1.8651490077482507 2308 58-59 -1.8300207953470604 2308 60-61 -3.3351244006475547 2308 62-63 -2.666662478925492 2308 64-65 -2.5809845441926917 2308 66-67 -2.0442667425213905 2308 68-69 -2.1291084963514777 2308 70-71 -2.258169296459503 2308 72-73 -2.2348645809478676 2308 74-75 -1.9751324613826569 2308 76-77 -1.784161390345318 2308 78-79 -1.8119728839757379 2308 80-81 -1.6918944695308085 2308 82-83 -1.6812993735114468 2308 84-85 -1.890383428725002 2308 86-87 -1.8772523187953958 2308 88-89 -1.6647929114433921 2308 90-91 -1.6684974302058997 2308 92-93 -1.640998398112341 2308 94-95 -1.7100009933596034 2308 96-97 -1.9786644750951545 2308 98-99 -2.023413850967099 2308 100 -1.9963714723983372 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 852900.0 3 243564.0 4 132421.0 5 106693.0 6 103857.0 7 114447.0 8 130384.0 9 142780.0 10 153088.0 11 155184.0 12 161381.0 13 191321.0 14 253775.0 15 348562.0 16 411428.0 17 377838.0 18 301365.0 19 233634.0 20 209499.0 21 209076.0 22 224726.0 23 255970.0 24 297540.0 25 356176.0 26 426842.0 27 510195.0 28 604426.0 29 715698.0 30 855853.0 31 1026451.0 32 1252647.0 33 1576345.0 34 2075754.0 35 2906481.0 36 4447001.0 37 6704731.0 38 6618001.0 39 1726159.0 40 4423.0 >>END_MODULE >>Per base sequence content pass #Base G A T C 1 22.1817871720051 28.176574425348853 27.45199599586641 22.189642406779637 2 23.892241028310405 27.813497179371844 27.237820873629182 21.056440918688573 3 23.6155768027337 26.41945388894127 26.940472624642236 23.024496683682795 4 24.492441444010623 26.137190795729897 26.650780444981503 22.719587315277977 5 24.74053022164155 26.21652281313665 26.155160308441122 22.88778665678068 6 24.773317517855883 25.939667110639586 25.725637562770924 23.5613778087336 7 24.505595582190505 26.276109725904384 25.991212090672615 23.227082601232492 8 24.044998892238752 26.016434419865835 26.27970744412888 23.65885924376654 9 24.046873867511785 25.76970179387202 26.369005202042846 23.81441913657335 10-11 24.064388766243 26.001693289197025 26.340830855568054 23.59308708899192 12-13 23.643043415432178 26.065492511144562 26.58365487275575 23.707809200667512 14-15 23.364719593894446 26.267074431353617 27.010588735489165 23.357617239262773 16-17 23.206819053167305 26.197949365985412 27.224644083418465 23.370587497428822 18-19 22.819293311225923 26.18381674582787 27.900203702456512 23.096686240489696 20-21 22.47931426273702 25.92827004191218 28.72452074288836 22.867894952462432 22-23 22.49084346390481 25.851997782951518 29.11256339542533 22.544595357718343 24-25 22.375438984283065 25.704337460093264 29.575194793857563 22.345028761766105 26-27 22.39531126314654 25.710702091418163 29.66963673078702 22.22434991464828 28-29 22.45470546794907 25.808040233774094 29.626977560124562 22.11027673815228 30-31 22.414039872536954 25.721035166961688 29.615409916823452 22.249515043677906 32-33 22.449156524331602 25.818109669549095 29.49371576261629 22.239018043503016 34-35 22.531070837548974 25.853814317019904 29.50272255169048 22.112392293740648 36-37 22.585783205561913 25.78449293520831 29.39949033433742 22.23023352489236 38-39 22.520912565159072 25.888928586323146 29.318491591511947 22.271667257005834 40-41 22.605116575919553 26.043001178131675 29.100047493541766 22.251834752407007 42-43 22.731641168941636 26.121385475620002 28.69111630665196 22.4558570487864 44-45 22.81424419416104 26.350660543975884 28.26284490094216 22.57225036092092 46-47 23.0292803800193 26.55102170594906 27.813633091131972 22.606064822899665 48-49 23.14363745994521 26.614526512046755 27.40602000282109 22.83581602518694 50-51 23.138000703461405 26.731051032861846 27.18194034282746 22.949007920849287 52-53 23.228448844468172 26.825016671309758 27.026208218298265 22.920326265923798 54-55 23.262690451262355 26.776068387672524 26.967714473136695 22.993526687928426 56-57 23.219808606916192 26.84476945492952 26.96115107375326 22.974270864401035 58-59 23.28832302383889 26.928814880521067 26.892600175343738 22.8902619202963 60-61 23.25458294051406 26.910175998801876 26.851198656326247 22.98404240435781 62-63 23.211660156040693 27.009688477430714 26.857718491830244 22.920932874698348 64-65 23.24298655024694 27.141272637373643 26.79944255278242 22.816298259596998 66-67 23.229524510168634 27.17746701960352 26.69921813199171 22.893790338236137 68-69 23.209173629936338 27.304041888992085 26.634039302633187 22.852745178438386 70-71 23.256574219353737 27.426751152275425 26.572685815250107 22.743988813120726 72-73 23.222224352875784 27.48061181353265 26.44706384172294 22.850099991868625 74-75 23.1759125334956 27.638603012296365 26.416741496295735 22.7687429579123 76-77 23.12374847636024 27.83459942964021 26.283808370539997 22.757843723459555 78-79 23.12692783731795 27.91141143360752 26.192864982680693 22.768795746393838 80-81 23.074699125733243 28.029380373251183 26.141912731139566 22.754007769876008 82-83 23.061096159126606 28.175786609619635 26.075173574320488 22.687943656933275 84-85 23.083544422603854 28.19417280300753 25.96767362377563 22.75460915061298 86-87 23.002319504475754 28.366177446334945 25.904637813020692 22.72686523616861 88-89 23.077442564036428 28.47663433151302 25.754995912657098 22.690927191793453 90-91 23.030197248958867 28.503986040390235 25.70614455949486 22.75967215115604 92-93 23.006944411502232 28.64417178610927 25.61275266519577 22.736131137192732 94-95 23.133910250853543 28.734140829356473 25.507117497046778 22.624831422743206 96-97 23.04070428511125 28.861981693379928 25.33955384601219 22.75776017549663 98-99 23.00181531455824 28.999242152308824 25.257634606229967 22.74130792690297 100 23.111624129253844 29.15945802854729 24.90945587033242 22.81946197186645 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 3484.0 1 2229.0 2 903.5 3 804.5 4 866.0 5 993.5 6 1161.0 7 1392.5 8 1576.0 9 1824.0 10 2378.0 11 3112.5 12 4199.0 13 6140.5 14 10582.0 15 15526.0 16 23246.0 17 27966.0 18 29474.5 19 36668.0 20 48233.0 21 61776.0 22 78085.0 23 99751.0 24 125518.5 25 154846.5 26 189061.0 27 230143.5 28 277957.0 29 326873.5 30 377734.5 31 437220.0 32 504638.5 33 577927.0 34 656411.5 35 740149.5 36 830939.0 37 937396.0 38 1050930.0 39 1141866.0 40 1210831.0 41 1283978.0 42 1385198.5 43 1489002.5 44 1565843.5 45 1614037.0 46 1613565.5 47 1559686.0 48 1522736.0 49 1536146.0 50 1470607.5 51 1349933.0 52 1224959.0 53 1078446.0 54 972207.0 55 893360.5 56 810889.5 57 731193.5 58 675568.5 59 628163.0 60 567586.5 61 510567.5 62 455960.5 63 397910.0 64 345987.5 65 296373.0 66 249183.0 67 206212.0 68 168400.5 69 136768.0 70 108220.0 71 83525.5 72 65064.0 73 51796.5 74 41262.0 75 30566.0 76 21293.5 77 15639.5 78 12139.5 79 8146.0 80 4266.5 81 2010.0 82 1058.5 83 625.5 84 399.0 85 297.0 86 214.0 87 149.0 88 108.5 89 82.5 90 58.0 91 43.5 92 33.0 93 24.5 94 17.5 95 13.0 96 12.0 97 7.0 98 2.0 99 1.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.24904181383940013 2 0.00237047783915899 3 0.0 4 7.215659713336271E-5 5 0.0 6 0.0011170910222868746 7 0.0016435669347043729 8 0.0021540080477589017 9 0.0026938462929788744 10-11 0.004006027374181878 12-13 0.00396326790921396 14-15 0.00386839534631639 16-17 0.003892447545360844 18-19 0.0034635166624014104 20-21 6.988500055694202E-4 22-23 0.0010676503909177186 24-25 7.41609470537339E-4 26-27 0.005268767824015725 28-29 0.0034635166624014104 30-31 0.0014845551743549253 32-33 0.002430608336770125 34-35 0.0010957112898029153 36-37 0.0035851138909039288 38-39 0.0022996574753058746 40-41 0.0023972025047639387 42-43 0.003973957775455939 44-45 2.68582889329739E-4 46-47 2.552205565272644E-4 48-49 0.0013201984808844881 50-51 0.0020978862499885084 52-53 0.0027793652229147117 54-55 0.0053168722221046336 56-57 8.979487643262915E-4 58-59 3.059974211766678E-4 60-61 2.4052199044454238E-5 62-63 1.1491606210128135E-4 64-65 0.0024132373041269085 66-67 6.413919745187796E-5 68-69 0.0030693278447284102 70-71 0.013745831753905598 72-73 0.004873242773062478 74-75 0.006707891066842237 76-77 0.018620410760248323 78-79 0.026171465026926705 80-81 0.02063945924670223 82-83 3.594467523865661E-4 84-85 2.137973248395932E-5 86-87 1.6970162659142712E-4 88-89 1.4030449442598304E-4 90-91 2.4453069028528473E-4 92-93 0.0010649779243572237 94-95 0.0038069286154250068 96-97 0.011589151239586199 98-99 0.0074468280708190815 100 0.009179922635300035 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 3.7418616E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 40.32525095476267 #Duplication Level Percentage of deduplicated Percentage of total 1 74.35381105507176 29.98336090238776 2 13.440696843512434 10.839989464430507 3 4.937627052918 5.973331500898308 4 2.2599557066116676 3.64533124063054 5 1.1851151466223102 2.389503284891751 6 0.7809826310319188 1.889599235260377 7 0.4624206311707805 1.3053059599035033 8 0.35074060936984075 1.131496247429217 9 0.2489569552441583 0.9035326527438872 >10 1.6055329401339344 13.317075059673389 >50 0.19751164208231084 5.339544032502664 >100 0.14732266477864808 13.515035909742837 >500 0.02554081110114932 7.068209550346574 >1k 0.003658740388015563 1.8737525026084507 >5k 3.996946202167446E-5 0.1306668899850028 >10k+ 8.660050095980102E-5 0.694265566565104 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 5.879426433088813E-5 5.34493312098983E-6 0.0 0.0 0.0 2 5.879426433088813E-5 8.017399681484745E-6 0.0 0.0 0.0 3 5.879426433088813E-5 8.017399681484745E-6 0.0 0.0 0.0 4 6.681166401237287E-5 8.017399681484745E-6 0.0 0.0 0.0 5 8.017399681484745E-5 8.017399681484745E-6 0.0 0.0 0.0 6 8.551892993583728E-5 8.017399681484745E-6 0.0 0.0 0.0 7 9.353632961732202E-5 1.068986624197966E-5 0.0 2.672466560494915E-6 0.0 8 9.620879617781695E-5 1.3362332802474576E-5 0.0 2.672466560494915E-6 2.672466560494915E-6 9 1.0422619585930169E-4 2.137973248395932E-5 2.672466560494915E-6 2.672466560494915E-6 2.672466560494915E-6 10-11 1.2560592834326103E-4 2.5388432324701695E-5 2.672466560494915E-6 5.34493312098983E-6 2.672466560494915E-6 12-13 2.6591042276924404E-4 3.073336544569152E-5 2.672466560494915E-6 5.34493312098983E-6 2.672466560494915E-6 14-15 5.157860461755187E-4 3.3405832006186436E-5 2.672466560494915E-6 6.681166401237288E-6 2.672466560494915E-6 16-17 9.874763941028711E-4 4.4095698248166105E-5 2.672466560494915E-6 8.017399681484745E-6 2.672466560494915E-6 18-19 0.001717059765117983 5.077686464940339E-5 2.672466560494915E-6 8.017399681484745E-6 2.672466560494915E-6 20-21 0.0027031999259406066 5.34493312098983E-5 4.008699840742372E-6 1.068986624197966E-5 2.672466560494915E-6 22-23 0.003995337507939898 5.7458031050640674E-5 5.34493312098983E-6 1.068986624197966E-5 2.672466560494915E-6 24-25 0.005553385512708434 6.28029641716305E-5 5.34493312098983E-6 1.068986624197966E-5 2.672466560494915E-6 26-27 0.007485578835946258 6.814789729262034E-5 5.34493312098983E-6 1.2026099522227119E-5 5.34493312098983E-6 28-29 0.009584801319215012 7.215659713336271E-5 5.34493312098983E-6 1.603479936296949E-5 5.34493312098983E-6 30-31 0.011154875423505777 7.482906369385763E-5 5.34493312098983E-6 2.137973248395932E-5 5.34493312098983E-6 32-33 0.012275975145633392 7.750153025435254E-5 5.34493312098983E-6 2.4052199044454238E-5 5.34493312098983E-6 34-35 0.013360996569194327 8.151023009509491E-5 5.34493312098983E-6 2.5388432324701695E-5 5.34493312098983E-6 36-37 0.014483432524602192 8.551892993583728E-5 5.34493312098983E-6 2.672466560494915E-5 5.34493312098983E-6 38-39 0.015612549646411294 8.81913964963322E-5 5.34493312098983E-6 2.672466560494915E-5 5.34493312098983E-6 40-41 0.01680179726583153 8.81913964963322E-5 5.34493312098983E-6 2.672466560494915E-5 6.681166401237288E-6 42-43 0.01805919278254439 9.220009633707457E-5 5.34493312098983E-6 2.672466560494915E-5 8.017399681484745E-6 44-45 0.01960922338763144 9.888126273831186E-5 5.34493312098983E-6 2.806089888519661E-5 8.017399681484745E-6 46-47 0.02139710351660254 1.0556242913954915E-4 5.34493312098983E-6 2.9397132165444065E-5 8.017399681484745E-6 48-49 0.02350300716627253 1.0823489570004406E-4 5.34493312098983E-6 2.9397132165444065E-5 8.017399681484745E-6 50-51 0.025979047434571072 1.1224359554078643E-4 5.34493312098983E-6 2.9397132165444065E-5 8.017399681484745E-6 52-53 0.02916863627452175 1.1491606210128135E-4 5.34493312098983E-6 2.9397132165444065E-5 8.017399681484745E-6 54-55 0.03299694462243072 1.25605928343261E-4 5.34493312098983E-6 3.206959872593898E-5 8.017399681484745E-6 56-57 0.03801850928960067 1.3095086146425083E-4 5.34493312098983E-6 3.206959872593898E-5 9.353632961732202E-6 58-59 0.04426406364147728 1.3495956130499321E-4 5.34493312098983E-6 3.47420652864339E-5 1.068986624197966E-5 60-61 0.051765677276786506 1.4297696098647796E-4 5.34493312098983E-6 3.47420652864339E-5 1.068986624197966E-5 62-63 0.06113534503788168 1.4698566082722034E-4 5.34493312098983E-6 3.7414531846928813E-5 1.068986624197966E-5 64-65 0.07310265029577791 1.4965812738771525E-4 5.34493312098983E-6 3.875076512717627E-5 1.068986624197966E-5 66-67 0.08820208636257418 1.536668272284576E-4 5.34493312098983E-6 4.275946496791864E-5 1.068986624197966E-5 68-69 0.10676771155833235 1.576755270692E-4 5.34493312098983E-6 4.5431931528413555E-5 1.068986624197966E-5 70-71 0.12910953200406985 1.6168422690994238E-4 5.34493312098983E-6 4.676816480866102E-5 1.068986624197966E-5 72-73 0.1566479636766897 1.697016265914271E-4 5.34493312098983E-6 4.8104398088908475E-5 1.068986624197966E-5 74-75 0.1905482019965677 1.7504655971241692E-4 5.34493312098983E-6 4.8104398088908475E-5 1.068986624197966E-5 76-77 0.23179104219140545 1.8039149283340675E-4 5.34493312098983E-6 4.944063136915593E-5 1.2026099522227119E-5 78-79 0.2819732830310987 1.89745125795139E-4 5.34493312098983E-6 5.612179777039322E-5 1.3362332802474576E-5 80-81 0.34162273666134524 1.924175923556339E-4 5.34493312098983E-6 6.681166401237287E-5 1.3362332802474576E-5 82-83 0.413477345073372 2.0043499203711864E-4 5.34493312098983E-6 6.814789729262034E-5 1.3362332802474576E-5 84-85 0.4987276921198796 2.0310745859761356E-4 5.34493312098983E-6 6.94841305728678E-5 1.3362332802474576E-5 86-87 0.5987380719800005 2.0577992515810847E-4 5.34493312098983E-6 6.94841305728678E-5 1.3362332802474576E-5 88 0.6849050750567578 2.0577992515810847E-4 5.34493312098983E-6 7.215659713336271E-5 1.3362332802474576E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ATCTCGA 26135 0.0 45.050835 1 TCTCGAG 32330 0.0 36.281933 2 TATCTCG 13665 0.0 29.576061 2 CTCGAGG 39350 0.0 29.43869 3 TCGAGGG 46220 0.0 24.921568 4 GATCCGG 43265 0.0 24.37151 1 ATCCGGC 50035 0.0 21.001457 2 ATATCTC 20625 0.0 20.102394 1 CGAGGGC 63635 0.0 18.153048 5 TCCGGCG 58425 0.0 18.073849 3 TATGGAT 26295 0.0 18.050938 2 AGGGCGC 67625 0.0 16.943172 7 CGGCGCG 65085 0.0 16.073408 5 GCGCCGT 65905 0.0 15.688415 9 GGGCGCG 73255 0.0 15.679502 8 GGATCCG 30595 0.0 15.329627 2 GAGGGCG 76405 0.0 15.125176 6 ATGGATC 31970 0.0 14.9494295 3 CCGGCGC 72490 0.0 14.567565 4 ATATGGA 35520 0.0 13.847986 1 >>END_MODULE