FastQCFastQC Report
Wed 25 May 2016
SRR491081_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR491081_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30482645
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA733880.24075338606607136No Hit
GATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT339740.1114535828501759No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCTCGA203500.044.315081
TCTCGAG246700.036.4606822
CTCGAGG313800.028.3791713
TATCTCG104450.026.950722
TCGAGGG357650.024.8217754
GATCCGG296100.024.759691
ATCCGGC343900.021.3588962
TCCGGCG404650.018.2216153
ATATCTC161450.017.9186121
CGAGGGC494800.017.8181935
AGGGCGC527100.016.5569427
GCGCCGT447500.016.099779
GGATCCG208800.015.8000552
CGGCGCG462800.015.7804075
GGGCGCG569650.015.4192228
GAGGGCG587950.015.0351916
TATGGAT214950.014.976322
CCGGCGC501300.014.6246684
CGTCGAC429750.013.18315512-13
ATGGATC247150.013.1009273
Produced by FastQC (version 0.11.5)