FastQCFastQC Report
Wed 25 May 2016
SRR491080_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR491080_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35226222
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA727440.20650525622645538No Hit
GATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT403340.11449993132956467No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCTCGA239150.042.1202771
TCTCGAG283300.035.1347162
CTCGAGG355800.027.6580563
TATCTCG127750.026.3028162
GATCCGG400200.025.1936231
TCGAGGG410950.023.8898434
ATCCGGC464500.021.5198332
TCCGGCG542050.018.475453
ATATCTC187200.018.4732861
TATGGAT243700.017.047232
CGAGGGC582850.016.9085165
GCGCCGT605750.016.2230269
GGATCCG280000.016.0792052
CGGCGCG625300.015.97865
AGGGCGC620850.015.6768417
CCGGCGC675000.014.9204624
GGGCGCG670750.014.5876448
CGTCGAC581550.014.21090212-13
ATGGATC301450.014.0350821
GAGGGCG708400.013.9715216
Produced by FastQC (version 0.11.5)