FastQCFastQC Report
Wed 25 May 2016
SRR491079_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR491079_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28843064
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCTCGA178450.041.5487251
TCTCGAG219300.033.6041222
CTCGAGG264850.027.4520913
TATCTCG97600.026.4848792
GATCCGG296650.025.8828891
TCGAGGG312150.022.9459864
ATCCGGC348200.021.8391062
TCCGGCG419800.018.1702633
ATATCTC148950.017.9313131
GGATCCG209900.016.5469192
CGAGGGC443150.016.406835
CGGCGCG465250.016.2032855
GCGCCGT462850.016.1454839
AGGGCGC468250.015.4172477
TATGGAT211650.015.144372
CCGGCGC522400.014.6195934
GGGCGCG503300.014.418298
CGTCGAC445750.014.03397912-13
ATGGATC244950.013.7498551
GAGGGCG538000.013.52304656
Produced by FastQC (version 0.11.5)