FastQCFastQC Report
Wed 25 May 2016
SRR491078_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR491078_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28783950
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCTCGA187350.039.629951
TATCTCG92750.033.6971472
TCTCGAG226350.032.682082
GATCCGG322400.027.3839191
CTCGAGG270100.027.1271843
ATCCGGC382450.023.1153032
TCGAGGG317450.022.8292794
ATATCTC159300.019.9326551
TCCGGCG454950.019.4419173
TATGGAT206850.017.3816642
CGGCGCG516900.017.0208995
CGAGGGC434200.016.7774145
GCGCCGT519100.016.7137139
GGATCCG216750.016.6311282
CCGGCGC545750.016.2416864
AGGGCGC480750.015.0065397
CGTCGAC495550.014.98991812-13
ATATGGA251050.014.7121751
ATGGATC258050.014.2765711
GCCGTCG525500.014.20714210-11
Produced by FastQC (version 0.11.5)