##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR491078_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	28783950
Sequences flagged as poor quality	0
Sequence length	100
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.317676448159478	31.0	31.0	34.0	26.0	34.0
2	30.629535765591587	31.0	31.0	34.0	27.0	34.0
3	30.734444994519514	31.0	31.0	34.0	27.0	34.0
4	34.20236816003363	37.0	35.0	37.0	30.0	37.0
5	33.80430663616356	37.0	35.0	37.0	28.0	37.0
6	33.71257051238624	37.0	35.0	37.0	28.0	37.0
7	33.636267815918245	37.0	35.0	37.0	28.0	37.0
8	33.5908179037276	37.0	35.0	37.0	28.0	37.0
9	35.0147567654891	39.0	35.0	39.0	28.0	39.0
10-11	34.93736924570811	39.0	35.0	39.0	27.5	39.0
12-13	34.88121734508294	39.0	35.0	39.0	27.0	39.0
14-15	35.96850461802498	40.0	36.0	41.0	27.0	41.0
16-17	35.90481282103394	40.0	36.0	41.0	27.0	41.0
18-19	35.876819946532706	40.0	36.0	41.0	27.0	41.0
20-21	35.827641932396354	40.0	36.0	41.0	27.0	41.0
22-23	35.71146734551721	39.5	36.0	41.0	27.0	41.0
24-25	35.5922833731993	39.0	35.0	41.0	27.0	41.0
26-27	35.41930489039899	39.0	35.0	41.0	26.0	41.0
28-29	35.19510796815587	39.0	35.0	41.0	25.0	41.0
30-31	35.013586894779905	39.0	34.0	41.0	25.0	41.0
32-33	34.78526420105649	39.0	34.0	41.0	24.0	41.0
34-35	34.62609216247249	38.5	34.0	41.0	24.0	41.0
36-37	34.4392897778102	38.0	34.0	40.0	23.0	41.0
38-39	34.235782076469704	38.0	33.5	40.0	21.5	41.0
40-41	33.97432723792252	38.0	33.0	40.0	18.5	41.0
42-43	33.63693605290448	38.0	33.0	40.0	15.5	41.0
44-45	33.30271964063306	38.0	33.0	40.0	11.5	41.0
46-47	33.162714029867345	38.0	33.0	40.0	8.5	41.0
48-49	32.99038954000406	38.0	32.0	40.0	7.5	41.0
50-51	32.74388297992458	38.0	32.0	40.0	3.5	41.0
52-53	32.514778756911404	38.0	31.5	40.0	2.0	41.0
54-55	32.26857752323778	37.0	31.0	40.0	2.0	41.0
56-57	31.987513440650083	37.0	31.0	40.0	2.0	41.0
58-59	31.69801239927112	36.5	31.0	40.0	2.0	41.0
60-61	31.402502505736706	36.0	30.0	40.0	2.0	41.0
62-63	31.15944100792282	36.0	30.0	39.0	2.0	41.0
64-65	31.0423395155981	35.5	30.0	39.0	2.0	41.0
66-67	30.833439399387508	35.0	30.0	39.0	2.0	41.0
68-69	30.523375023233434	35.0	30.0	39.0	2.0	40.5
70-71	30.1680792768192	35.0	29.0	38.0	2.0	40.0
72-73	29.794018663178612	35.0	29.0	37.0	2.0	40.0
74-75	29.435109131998907	35.0	29.0	37.0	2.0	39.0
76-77	28.618521398209765	34.0	27.5	36.0	2.0	39.0
78-79	28.76141012612932	34.0	29.0	36.0	2.0	39.0
80-81	28.478991938215568	34.0	28.0	35.5	2.0	37.0
82-83	28.23070445508695	34.0	28.0	35.0	2.0	37.0
84-85	27.94247889883077	34.0	27.0	35.0	2.0	36.5
86-87	27.629642717556138	34.0	27.0	35.0	2.0	36.0
88-89	27.376615405460335	34.0	26.0	35.0	2.0	36.0
90-91	27.054244448729243	34.0	26.0	35.0	2.0	36.0
92-93	26.82850658092444	34.0	25.0	35.0	2.0	35.0
94-95	26.452482425101486	34.0	25.0	35.0	2.0	35.0
96-97	26.20904450223128	34.0	24.0	35.0	2.0	35.0
98-99	25.859861850093544	34.0	23.5	35.0	2.0	35.0
100	25.11495753709967	33.0	18.0	35.0	2.0	35.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	0.6780724516713406
1101	2	0.6727353018829589
1101	3	0.6434038560118829
1101	4	0.7381309495971067
1101	5	0.9484588442196085
1101	6	0.9535707836216218
1101	7	0.9009673038862545
1101	8	0.9814719424301614
1101	9	1.007422643005519
1101	10-11	1.0847522576045563
1101	12-13	1.086987443978309
1101	14-15	1.1924875157492494
1101	16-17	1.1644194992639783
1101	18-19	1.120300937185469
1101	20-21	1.1986473674997526
1101	22-23	1.2074189884132593
1101	24-25	1.2646450106953964
1101	26-27	1.3028230462165027
1101	28-29	1.3435619066039877
1101	30-31	1.3738314598006696
1101	32-33	1.293374269889192
1101	34-35	1.3125348889616362
1101	36-37	1.2960667044234881
1101	38-39	1.3167649967247783
1101	40-41	1.2908978078560054
1101	42-43	1.252995497251277
1101	44-45	1.3447716137999919
1101	46-47	1.125344104399744
1101	48-49	1.1839414905344157
1101	50-51	1.0972397336737387
1101	52-53	1.2209586731605313
1101	54-55	1.1563480195716807
1101	56-57	1.1634791602028045
1101	58-59	1.0717801944893068
1101	60-61	1.1012411128640416
1101	62-63	1.125184908232388
1101	64-65	1.2605276752189845
1101	66-67	1.1477356884054473
1101	68-69	1.1153765476779505
1101	70-71	1.0723591980236336
1101	72-73	1.1046651815879933
1101	74-75	1.1014995589944583
1101	76-77	0.9758611508047501
1101	78-79	1.1010164943262808
1101	80-81	1.1399743773368272
1101	82-83	1.143238553694971
1101	84-85	1.069267315524577
1101	86-87	0.994213168069578
1101	88-89	1.036392912935721
1101	90-91	1.1325417445848487
1101	92-93	1.1579434017602281
1101	94-95	1.224210552458974
1101	96-97	1.0730115308375012
1101	98-99	0.9967933665354138
1101	100	0.9987772312700187
1102	1	0.42529501824523663
1102	2	0.4520058879405262
1102	3	0.4480956369447071
1102	4	0.4899339934933522
1102	5	0.48147552469557553
1102	6	0.6125022922660932
1102	7	0.6044151727594311
1102	8	0.6741497968750991
1102	9	0.6208976317303225
1102	10-11	0.749067630456274
1102	12-13	0.7646177928534215
1102	14-15	0.7978526536674195
1102	16-17	0.6373067048540264
1102	18-19	0.7256187630387458
1102	20-21	0.7411401355328309
1102	22-23	0.7225276543824677
1102	24-25	0.75952660983436
1102	26-27	0.7208579016415726
1102	28-29	0.7655596252385166
1102	30-31	0.7309076748372831
1102	32-33	0.7337851562682332
1102	34-35	0.7696842408299958
1102	36-37	0.7933350220516573
1102	38-39	0.8015842395090331
1102	40-41	0.7697354534115348
1102	42-43	0.7343909284783763
1102	44-45	0.7406319333448863
1102	46-47	0.7444510124609209
1102	48-49	0.7132160473295457
1102	50-51	0.6099644941897466
1102	52-53	0.598389547637538
1102	54-55	0.5714122968411814
1102	56-57	0.6969557440695482
1102	58-59	0.6779223931990401
1102	60-61	0.6014523735375583
1102	62-63	0.5971081436064516
1102	64-65	0.7194847913477993
1102	66-67	0.7353314488599878
1102	68-69	0.7387958487028108
1102	70-71	0.7365027445346648
1102	72-73	0.6826133023790106
1102	74-75	0.6951589717034317
1102	76-77	0.6947354278559814
1102	78-79	0.6069757935878926
1102	80-81	0.7687433129286099
1102	82-83	0.7352206123569296
1102	84-85	0.6990901713662652
1102	86-87	0.6582041781688162
1102	88-89	0.5696218753646249
1102	90-91	0.5702908292839695
1102	92-93	0.661708929678376
1102	94-95	0.8364499650518056
1102	96-97	0.7105127486183918
1102	98-99	0.6336934552379105
1102	100	0.6176668357733526
1103	1	0.2043479975267637
1103	2	0.16413541048917324
1103	3	0.190312390064598
1103	4	0.28625029259549706
1103	5	0.3787543929259556
1103	6	0.3036966466228179
1103	7	0.30603073111189616
1103	8	0.37073907420287355
1103	9	0.4377362099223774
1103	10-11	0.42500282897334785
1103	12-13	0.3671806181670618
1103	14-15	0.3610523101447356
1103	16-17	0.358387114586435
1103	18-19	0.44512761387093036
1103	20-21	0.4401457304454581
1103	22-23	0.3824173502989865
1103	24-25	0.37580417659721377
1103	26-27	0.3205091218477705
1103	28-29	0.40754941793588984
1103	30-31	0.3914660578739344
1103	32-33	0.3844985996321668
1103	34-35	0.4051525989991589
1103	36-37	0.43149561349765264
1103	38-39	0.47432700121893845
1103	40-41	0.4300508973032109
1103	42-43	0.4120696829034358
1103	44-45	0.454239901759955
1103	46-47	0.37516319794819
1103	48-49	0.43554068072199925
1103	50-51	0.46705877642074967
1103	52-53	0.4742600250971236
1103	54-55	0.33670337021339947
1103	56-57	0.31209192678310416
1103	58-59	0.4406672209283258
1103	60-61	0.3733364634071421
1103	62-63	0.5452940304890816
1103	64-65	0.5646480585791664
1103	66-67	0.4683711763827674
1103	68-69	0.4613851168918899
1103	70-71	0.4730039360766014
1103	72-73	0.4460166940997006
1103	74-75	0.5785619894207592
1103	76-77	0.5436431612971901
1103	78-79	0.5145951970998617
1103	80-81	0.564110650749349
1103	82-83	0.5650816569903263
1103	84-85	0.48428736413266193
1103	86-87	0.5777326356322376
1103	88-89	0.6037014015817093
1103	90-91	0.4403306263365181
1103	92-93	0.5588596669349073
1103	94-95	0.6980134856878948
1103	96-97	0.5922784857919474
1103	98-99	0.554515100477385
1103	100	0.594657427087963
1104	1	-0.14745132165612418
1104	2	-0.0985196046515
1104	3	-0.0670483727291149
1104	4	-0.037362482351717574
1104	5	-0.024217357761301628
1104	6	-0.05801529156012464
1104	7	-0.02227971393237027
1104	8	-0.056857246921815374
1104	9	-0.13999075769680047
1104	10-11	-0.17432231804595943
1104	12-13	-0.08396381063464986
1104	14-15	-0.1285280983329713
1104	16-17	-0.14505470896335027
1104	18-19	-0.25230517559574395
1104	20-21	-0.1982642929457299
1104	22-23	-0.20806240235177853
1104	24-25	-0.19494178486024794
1104	26-27	-0.11612649195171088
1104	28-29	-0.32025389258469517
1104	30-31	-0.19048120416098868
1104	32-33	-0.34303939030579755
1104	34-35	-0.20667866900102894
1104	36-37	-0.38721343496717253
1104	38-39	-0.40936996255183544
1104	40-41	-0.32183823628593444
1104	42-43	-0.21412379125091974
1104	44-45	-0.3520348637345663
1104	46-47	-0.22764751239581216
1104	48-49	-0.2060871192122491
1104	50-51	-0.20803375423820825
1104	52-53	-0.20105606447945945
1104	54-55	-0.2223191119609993
1104	56-57	-0.21566887742999796
1104	58-59	-0.39834268446499266
1104	60-61	-0.35993521912030957
1104	62-63	-0.1265672361793797
1104	64-65	-0.11527186350944874
1104	66-67	-0.22284570868391285
1104	68-69	-0.2378891549311497
1104	70-71	-0.2535163181549436
1104	72-73	-0.20939965888999623
1104	74-75	-0.2822813892770988
1104	76-77	-0.2719696399087361
1104	78-79	-0.2747205607527192
1104	80-81	-0.2396189341998749
1104	82-83	-0.2101039185380671
1104	84-85	-0.22918240688476743
1104	86-87	-0.18862934643261298
1104	88-89	-0.14555176198137687
1104	90-91	-0.11280665511385024
1104	92-93	-0.09606174345760365
1104	94-95	0.06949772828759038
1104	96-97	0.005373966284924592
1104	98-99	-0.04037874801335661
1104	100	0.0011331400973695338
1105	1	-0.48210297273308456
1105	2	-0.471728513847129
1105	3	-0.3991413537124018
1105	4	-0.3394646855551642
1105	5	-0.529717367379611
1105	6	-0.590202602077639
1105	7	-0.6241850425754691
1105	8	-0.6355168328560552
1105	9	-0.6015867980479896
1105	10-11	-0.5647611762418308
1105	12-13	-0.5910766551138877
1105	14-15	-0.7223000012832941
1105	16-17	-0.6131144599101717
1105	18-19	-0.6313724940229903
1105	20-21	-0.6781652038564729
1105	22-23	-0.6729981568325982
1105	24-25	-0.5596348784310052
1105	26-27	-0.5429676964579997
1105	28-29	-0.5961057780467343
1105	30-31	-0.6125803422823353
1105	32-33	-0.6574916343852806
1105	34-35	-0.6050510091656562
1105	36-37	-0.6005008138669794
1105	38-39	-0.5919634421433386
1105	40-41	-0.5464848348220741
1105	42-43	-0.6278801479422853
1105	44-45	-0.4967255584065313
1105	46-47	-0.6988100910973714
1105	48-49	-0.6842483094344516
1105	50-51	-0.5560526467994933
1105	52-53	-0.5679224635453437
1105	54-55	-0.5605903775297065
1105	56-57	-0.48462901088387156
1105	58-59	-0.5794714635912577
1105	60-61	-0.5264560125222779
1105	62-63	-0.5977389429587809
1105	64-65	-0.6782169587889264
1105	66-67	-0.6632323174516657
1105	68-69	-0.7016199232800808
1105	70-71	-0.5647045434944111
1105	72-73	-0.5653869508132949
1105	74-75	-0.5875074935620077
1105	76-77	-0.5636866437285164
1105	78-79	-0.52643792375072
1105	80-81	-0.43442964168939824
1105	82-83	-0.4181132010412476
1105	84-85	-0.5118730283014159
1105	86-87	-0.4041042409044877
1105	88-89	-0.448967911507129
1105	90-91	-0.436577311321809
1105	92-93	-0.35547939542725615
1105	94-95	-0.26737266015868144
1105	96-97	-0.4075848093546135
1105	98-99	-0.4103567369014023
1105	100	-0.31911401373468706
1106	1	-0.7622825876375039
1106	2	-0.7054516571276679
1106	3	-0.6709193957265853
1106	4	-0.6247348445185565
1106	5	-0.6700904467499527
1106	6	-0.679956975509441
1106	7	-0.6786732821182895
1106	8	-0.7110888344271586
1106	9	-0.8852817309384449
1106	10-11	-0.9444571052908444
1106	12-13	-0.9245876075368713
1106	14-15	-1.1270937461910862
1106	16-17	-1.166248334473643
1106	18-19	-1.0578929305089133
1106	20-21	-1.1425201183824427
1106	22-23	-1.098191511470624
1106	24-25	-1.0762008491708386
1106	26-27	-1.1156304989067607
1106	28-29	-1.1564088916266186
1106	30-31	-1.1598541216923195
1106	32-33	-1.0787083651599403
1106	34-35	-1.0512752929511322
1106	36-37	-1.104005678652129
1106	38-39	-1.120101703876685
1106	40-41	-1.1696352294841503
1106	42-43	-1.1999788736009052
1106	44-45	-1.1209895114710946
1106	46-47	-1.0888720984268545
1106	48-49	-1.041334808481082
1106	50-51	-1.0634556066896579
1106	52-53	-1.101501548062263
1106	54-55	-1.033322439375425
1106	56-57	-0.9806650084606545
1106	58-59	-1.0224229879935613
1106	60-61	-0.9858535223528513
1106	62-63	-1.0146400411522585
1106	64-65	-0.9961989791261878
1106	66-67	-1.058127109887387
1106	68-69	-1.1209161589329355
1106	70-71	-1.0642761633102857
1106	72-73	-1.0363128819542986
1106	74-75	-0.9984272606351254
1106	76-77	-0.9880631224142782
1106	78-79	-0.9984611366350151
1106	80-81	-0.8625322927005534
1106	82-83	-0.9332376076058004
1106	84-85	-0.9132943156352624
1106	86-87	-0.8510380911909436
1106	88-89	-0.8313826226191701
1106	90-91	-0.8169785238056342
1106	92-93	-0.8136068088493751
1106	94-95	-0.6969508204080661
1106	96-97	-0.801638332516557
1106	98-99	-0.8714127895273336
1106	100	-0.8379510616627961
1107	1	-1.1789827583587211
1107	2	-1.1449274133039182
1107	3	-1.130767396667558
1107	4	-1.1451324880492635
1107	5	-1.2650001985607275
1107	6	-1.3179210653027909
1107	7	-1.359815776292919
1107	8	-1.4305410890270238
1107	9	-1.5650669587074404
1107	10-11	-1.6544872485817592
1107	12-13	-1.6990308206487228
1107	14-15	-1.9357693393743176
1107	16-17	-1.9266546470411399
1107	18-19	-1.935916780444991
1107	20-21	-1.9948813264047658
1107	22-23	-2.2029951355274093
1107	24-25	-2.0997827928239943
1107	26-27	-2.0503873698656463
1107	28-29	-2.064370606849529
1107	30-31	-1.96151269000665
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1307	84-85	-1.8036527141702265
1307	86-87	-1.853166513533722
1307	88-89	-1.721550723078611
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1307	92-93	-1.8135527955728108
1307	94-95	-1.633029124069953
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1308	1	-1.807733816418235
1308	2	-1.7050141271310615
1308	3	-1.6513898027373415
1308	4	-1.6932226950958338
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1308	8	-2.053702804656993
1308	9	-2.382707100457864
1308	10-11	-2.364913615093137
1308	12-13	-2.4270979363826157
1308	14-15	-2.6596046697483473
1308	16-17	-2.7135031206906106
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1308	22-23	-2.9184431978506993
1308	24-25	-2.870009100915418
1308	26-27	-2.844601276274865
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1308	34-35	-2.957100516250623
1308	36-37	-3.196584159908987
1308	38-39	-3.3587974487074455
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1308	54-55	-2.8629801075723087
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1308	96-97	-3.218931751042902
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2101	1	1.4154927997488933
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2101	14-15	2.0478013565636317
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2101	22-23	2.083641698608517
2101	24-25	2.1100129190011145
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2101	28-29	2.1481695547058095
2101	30-31	2.14059387841683
2101	32-33	2.2141245838958667
2101	34-35	2.188666992834264
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2101	64-65	2.2530504765385437
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2101	70-71	2.182686536403935
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2101	74-75	2.2168722717067517
2101	76-77	2.199893406529437
2101	78-79	2.1488221540909507
2101	80-81	2.1812892170572837
2101	82-83	2.130315153616923
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2101	94-95	2.4009716544111868
2101	96-97	2.306776607281474
2101	98-99	2.3132467302829625
2101	100	2.3676100149932537
2102	1	1.0911424229795443
2102	2	1.0647532210367068
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2102	4	0.9427034981234073
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2102	9	1.3322649511256728
2102	10-11	1.3765577475149158
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2102	14-15	1.563219921556204
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2102	32-33	1.6006926476132435
2102	34-35	1.6246871786372807
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2106	98-99	0.14957051669501453
2106	100	0.20005969678904023
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2107	94-95	-0.9922407630237196
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2108	9	-2.564646910513744
2108	10-11	-2.456261738252593
2108	12-13	-2.5090491635649173
2108	14-15	-2.808911653143973
2108	16-17	-2.7632736589120768
2108	18-19	-2.7933769044506107
2108	20-21	-2.8664838421985124
2108	22-23	-2.991310438229256
2108	24-25	-3.1420936884996067
2108	26-27	-3.0020643177173056
2108	28-29	-3.0631287252846917
2108	30-31	-2.937504053030345
2108	32-33	-3.0011273671703407
2108	34-35	-3.0180247214029094
2108	36-37	-2.9575983401245836
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2108	40-41	-3.2048883397320864
2108	42-43	-3.4401192550387947
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2108	48-49	-2.9218444591498596
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2108	56-57	-3.079056286173781
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2108	64-65	-3.175373846297351
2108	66-67	-3.2389279729865414
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2108	74-75	-3.227963959755904
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2108	78-79	-3.1602508051892535
2108	80-81	-2.9923457338193913
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2202	3	1.070317004049926
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2202	9	1.4488366347652146
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2202	22-23	1.7282467590527517
2202	24-25	1.7040526691538318
2202	26-27	1.645701495030707
2202	28-29	1.7607847749311887
2202	30-31	1.7326860399721937
2202	32-33	1.7053849545514765
2202	34-35	1.7212711755581935
2202	36-37	1.712899323810852
2202	38-39	1.6909751895651794
2202	40-41	1.7799400189935781
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2202	48-49	1.7329551250919693
2202	50-51	1.6815898468642345
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2202	58-59	1.6539261222916792
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2202	62-63	1.7192335371962564
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2202	66-67	1.73090462943334
2202	68-69	1.7198139059666069
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2202	72-73	1.7575997447043612
2202	74-75	1.6877545453228713
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2203	1	0.9887787404295665
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2203	5	1.0058497209346697
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2203	7	1.0258968866648175
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2203	9	1.2024270670472674
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2203	14-15	1.3242156212139164
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2203	24-25	1.3721884954846857
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2203	28-29	1.5042883527919955
2203	30-31	1.4961155927807113
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2203	36-37	1.5392495441488379
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2206	100	0.21457468936020518
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2207	10-11	-0.7063535405026684
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2207	78-79	-1.2008664138656826
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2207	82-83	-1.3758818775496273
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2207	86-87	-1.2445191845545978
2207	88-89	-1.325971303716674
2207	90-91	-1.2104424994005925
2207	92-93	-1.1934064077982356
2207	94-95	-1.078658606893665
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2207	100	-1.3110317131318716
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2208	2	-1.5636672412440902
2208	3	-1.5193626589109748
2208	4	-1.537811845319581
2208	5	-1.9356816907362102
2208	6	-1.8321527600210814
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2208	8	-2.0033639216202204
2208	9	-2.1744560216360256
2208	10-11	-2.116298090520992
2208	12-13	-2.183961267398935
2208	14-15	-2.4528249155844435
2208	16-17	-2.4139728806893928
2208	18-19	-2.5349905400758246
2208	20-21	-2.5994739093767905
2208	22-23	-2.7549829567253212
2208	24-25	-2.8317532775168743
2208	26-27	-2.5513710732314294
2208	28-29	-2.4838828965356043
2208	30-31	-2.6305007208963502
2208	32-33	-2.8411558579485643
2208	34-35	-2.6639410358224964
2208	36-37	-2.558673489794394
2208	38-39	-2.6211611054389934
2208	40-41	-2.599221770661778
2208	42-43	-2.6121461679614697
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2308	80-81	-2.5741932795528015
2308	82-83	-2.387868431204989
2308	84-85	-2.273774774589981
2308	86-87	-2.488869231823223
2308	88-89	-2.61611587098394
2308	90-91	-2.662842430259591
2308	92-93	-2.4457583029785397
2308	94-95	-2.1530161591200283
2308	96-97	-2.3347934795630465
2308	98-99	-2.5536707748731367
2308	100	-2.919941423979111
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	782504.0
3	219589.0
4	117590.0
5	94306.0
6	91609.0
7	100581.0
8	112949.0
9	123331.0
10	132597.0
11	134052.0
12	141347.0
13	167342.0
14	218278.0
15	283937.0
16	324980.0
17	292069.0
18	236826.0
19	191610.0
20	175540.0
21	177119.0
22	190413.0
23	218114.0
24	256068.0
25	304366.0
26	363599.0
27	431713.0
28	511667.0
29	604937.0
30	716427.0
31	855676.0
32	1037288.0
33	1288935.0
34	1659547.0
35	2239499.0
36	3242704.0
37	4683653.0
38	4711506.0
39	1346494.0
40	3188.0
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	21.67196632558866	28.479978280145378	27.738564662658803	22.109490731607156
2	23.6786517895415	27.992047393233864	27.35786153728672	20.971439279937915
3	23.452573395937666	26.708891587151868	27.03626847600833	22.802266540902135
4	24.182129435092676	26.45772944447291	26.717263194647707	22.642877925786703
5	24.54991202034691	26.423666012505315	26.34736996284871	22.679052004299066
6	24.55925627570098	26.195277274685928	25.88505631160327	23.36041013800982
7	24.36319773706185	26.518924548484552	26.101669617312023	23.016208097141572
8	23.825212019650337	26.26821789533612	26.434217466873918	23.472352618139627
9	23.875453889421053	26.040944638954006	26.498841802403533	23.584759669221405
10-11	23.78979335064038	26.28764919474571	26.526665836411194	23.395891618202718
12-13	23.417559494504054	26.329078941891567	26.724789393482556	23.528572170121826
14-15	23.15808363614443	26.51050433270174	27.200739914727052	23.13067211642678
16-17	23.00195582613764	26.465281845129173	27.407164617717534	23.125597711015658
18-19	22.62803689685158	26.395707891882175	28.078462430721647	22.8977927805446
20-21	22.345923652079218	26.161817110983087	28.868735207076824	22.62352402986087
22-23	22.309835222083137	26.104773588006218	29.212064930052158	22.37332625985848
24-25	22.227059672953203	25.953335251560215	29.605186313865783	22.214418761620795
26-27	22.27898848894924	25.957542284367	29.71315883032079	22.050310396362967
28-29	22.30078937428448	26.064398305350906	29.665115929707024	21.969696390657585
30-31	22.307349097983487	25.961010401632663	29.663423251404158	22.068217248979696
32-33	22.336772624790203	26.031284108744696	29.560964471899855	22.07097879456525
34-35	22.37693470558956	26.107051494489426	29.543228770590297	21.972785029330723
36-37	22.37696620875318	26.06333836773197	29.470133596856403	22.089561826658446
38-39	22.413127375568536	26.110719646399083	29.3663211972247	22.109831780807678
40-41	22.51593547549473	26.2702581461738	29.131849893728756	22.081956484602717
42-43	22.654788028105315	26.36632059375544	28.70976546946573	22.269125908673516
44-45	22.765200395666238	26.55195850062618	28.296881071111528	22.38596003259605
46-47	22.940220061978863	26.79391118882986	27.84051734852454	22.425351400666738
48-49	23.048714050417825	26.836222758890788	27.46516445790296	22.649898732788422
50-51	23.071083330477244	26.975614397133945	27.25531611048065	22.697986161908165
52-53	23.156750863375148	27.066043074328434	27.106856319450262	22.670349742846156
54-55	23.16627323656118	27.005755699090912	27.058963479433768	22.769007584914135
56-57	23.121685509764195	27.03912869437356	27.066393995130184	22.772791800732065
58-59	23.14043858721309	27.130953160363408	27.021765554057403	22.7068426983661
60-61	23.086764474722095	27.139610056349184	26.976780761820557	22.796844707108164
62-63	23.03475220281114	27.180557382386862	27.021645640677903	22.76304477412409
64-65	23.083916878742215	27.35691438664427	26.908484064746357	22.650684669867157
66-67	23.070485147173994	27.359789282212482	26.81444054995803	22.755285020655496
68-69	23.04640840314729	27.491489855329455	26.730372921901225	22.731728819622038
70-71	23.13568570768374	27.62705421236587	26.627266103866997	22.609993976083395
72-73	23.127486954594925	27.706406939094485	26.489139790168725	22.676966316141865
74-75	23.116653700523752	27.82494779467119	26.482157289174356	22.576241215630702
76-77	23.03502862074285	28.0462781916749	26.343925926543566	22.574767261038687
78-79	23.02928238307165	28.12253943851767	26.26333629426635	22.584841884144332
80-81	22.969776304241535	28.23100772125632	26.208379767684526	22.590836206817617
82-83	22.970963911383834	28.385320315871216	26.124235430585006	22.51948034215994
84-85	22.95057402830391	28.42389519471756	26.036828863580958	22.588701913397568
86-87	22.90536363565773	28.554225530095046	25.970710989548333	22.56969984469889
88-89	22.940347961250858	28.686827925702207	25.853223194590992	22.51960091845594
90-91	22.90954567037577	28.719114767666188	25.755033338448598	22.61630622350944
92-93	22.866868942048153	28.820781589350307	25.729191586591128	22.58315788201041
94-95	22.91283864172768	28.973254726791648	25.582443021956646	22.531463609524025
96-97	22.855933573947517	29.05687450198961	25.46039654612043	22.62679537794244
98-99	22.848908265144274	29.168613676454534	25.367254395528764	22.615223662872427
100	22.903500156512898	29.327741485854602	25.020680200544966	22.748078157087534
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2805.0
1	1769.5
2	660.5
3	572.0
4	608.0
5	707.0
6	830.5
7	934.0
8	1105.0
9	1385.5
10	1696.5
11	2177.0
12	2860.5
13	4051.0
14	6934.0
15	10288.5
16	15511.0
17	18992.0
18	21037.5
19	27427.0
20	37490.5
21	49770.5
22	65622.0
23	87051.0
24	112244.5
25	140842.5
26	174473.0
27	214962.0
28	261368.5
29	308609.0
30	355475.5
31	404536.5
32	457377.5
33	514556.5
34	574161.5
35	636307.5
36	701636.5
37	772784.5
38	839012.5
39	889193.0
40	929973.5
41	969236.5
42	1016176.5
43	1060310.5
44	1094650.5
45	1113626.5
46	1107714.5
47	1086996.5
48	1073321.5
49	1071345.5
50	1038273.5
51	981041.5
52	913990.0
53	834541.0
54	771565.0
55	719677.0
56	661525.0
57	603643.0
58	556937.5
59	513214.0
60	462632.0
61	414620.0
62	368125.5
63	317597.0
64	270830.0
65	227011.5
66	186974.0
67	153391.0
68	124880.5
69	100465.0
70	79176.0
71	61140.0
72	47160.0
73	37209.0
74	29343.0
75	21542.0
76	14810.0
77	10702.5
78	8192.0
79	5452.5
80	2847.5
81	1436.5
82	799.5
83	480.5
84	310.0
85	199.5
86	133.5
87	86.0
88	57.0
89	39.0
90	29.5
91	28.5
92	20.5
93	15.0
94	13.0
95	6.5
96	2.0
97	3.0
98	4.0
99	1.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.24044302467173548
2	0.0015008364036207679
3	0.0
4	8.685395854286851E-5
5	7.643148351772429E-5
6	9.484452272881241E-4
7	0.005371048796290989
8	0.006767660449660314
9	0.007504182018103839
10-11	0.00571325339294989
12-13	0.008866052088056017
14-15	0.00783422706056674
16-17	0.004266266443625701
18-19	0.001801351100179093
20-21	4.429551885686294E-4
22-23	0.0012506970030173065
24-25	0.00147304313688705
26-27	0.0031719065659855574
28-29	0.0022443062887477225
30-31	8.598541895743982E-4
32-33	0.0019125241671139645
34-35	0.0010665666109064252
36-37	0.002216513022014004
38-39	0.0010908857192984283
40-41	0.0018378297627670976
42-43	0.002965194144653531
44-45	3.265708841211856E-4
46-47	3.6304954670919034E-4
48-49	6.983058266846629E-4
50-51	0.001492151007766481
52-53	0.0013966116533693257
54-55	0.0019646365422396855
56-57	3.891057342720509E-4
58-59	1.910787087943107E-5
60-61	4.325327135434852E-4
62-63	1.6675960040230754E-4
64-65	0.002680313160632922
66-67	0.00280711994010551
68-69	2.3624276723660233E-4
70-71	0.0012194295779418738
72-73	8.754879021121145E-4
74-75	3.821574175886214E-5
76-77	0.0021887197552802862
78-79	0.010577075071350528
80-81	0.02193757284875773
82-83	0.001773557833445375
84-85	0.001525155512012771
86-87	0.002895710977819236
88-89	0.002987776173874677
90-91	0.0028383873651809427
92-93	3.0572593407089714E-4
94-95	0.0011829509153538692
96-97	0.0023103152972403025
98-99	0.0017231825374905113
100	0.0013201801698516013
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	2.878395E7
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	53.665570766570525
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.79496053634722	42.822421014790436
2	11.666703160302156	12.522005681235346
3	3.4412355717993863	5.540276133085193
4	1.5562043916515829	3.340583876297034
5	0.8396901293689016	2.2531225029818773
6	0.511433086083897	1.646780908416056
7	0.3276602670628599	1.2308852674618715
8	0.22705441813431496	0.9748003955399657
9	0.1693272793662062	0.8178340584184197
>10	1.2439548022153708	13.415969597418865
>50	0.1230657410541497	4.585932263741187
>100	0.09757261707449463	9.618551187501847
>500	7.916786081678728E-4	0.2693515651856875
>1k	2.3576454880532767E-4	0.21932812058047263
>5k	3.9019899736768325E-5	0.14155959289517497
>10k+	7.153648277934745E-5	0.6005978344505719
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	3.4741583417147405E-6	0.0	0.0	0.0
2	0.0	3.4741583417147405E-6	0.0	0.0	0.0
3	0.0	3.4741583417147405E-6	0.0	0.0	0.0
4	0.0	6.948316683429481E-6	0.0	0.0	3.4741583417147405E-6
5	3.4741583417147405E-6	6.948316683429481E-6	0.0	0.0	3.4741583417147405E-6
6	3.4741583417147405E-6	1.3896633366858962E-5	0.0	3.4741583417147405E-6	3.4741583417147405E-6
7	3.4741583417147405E-6	1.3896633366858962E-5	0.0	3.4741583417147405E-6	3.4741583417147405E-6
8	6.948316683429481E-6	1.3896633366858962E-5	0.0	6.948316683429481E-6	3.4741583417147405E-6
9	1.73707917085737E-5	1.3896633366858962E-5	0.0	6.948316683429481E-6	3.4741583417147405E-6
10-11	3.647866258800477E-5	1.563371253771633E-5	0.0	1.0422475025144221E-5	3.4741583417147405E-6
12-13	1.3549217532687487E-4	1.73707917085737E-5	0.0	1.2159554196001591E-5	3.4741583417147405E-6
14-15	3.5957538836747566E-4	1.73707917085737E-5	0.0	1.910787087943107E-5	3.4741583417147405E-6
16-17	8.476946353783966E-4	1.910787087943107E-5	0.0	2.4319108392003183E-5	6.948316683429481E-6
18-19	0.0015581600162590612	2.0844950050288442E-5	0.0	2.7793266733717924E-5	6.948316683429481E-6
20-21	0.002541346826964333	2.0844950050288442E-5	0.0	2.7793266733717924E-5	6.948316683429481E-6
22-23	0.003750353929881062	2.4319108392003183E-5	0.0	2.953034590457529E-5	1.0422475025144221E-5
24-25	0.005169547612471534	2.6056187562860553E-5	0.0	3.126742507543266E-5	1.0422475025144221E-5
26-27	0.006845829012348896	2.7793266733717924E-5	0.0	3.821574175886214E-5	1.3896633366858962E-5
28-29	0.008687132933457709	3.126742507543266E-5	0.0	4.3426979271434254E-5	1.3896633366858962E-5
30-31	0.010002101865796737	3.821574175886214E-5	0.0	4.5164058442291625E-5	1.73707917085737E-5
32-33	0.01095749540976829	3.821574175886214E-5	0.0	4.6901137613148995E-5	1.73707917085737E-5
34-35	0.012022324941503858	4.6901137613148995E-5	0.0	5.384945429657848E-5	1.73707917085737E-5
36-37	0.013012460068892558	5.0375295954863736E-5	0.0	6.253485015086532E-5	1.73707917085737E-5
38-39	0.013964379454522398	5.558653346743585E-5	0.0	6.253485015086532E-5	2.0844950050288442E-5
40-41	0.014886768494247662	5.558653346743585E-5	0.0	6.427192932172269E-5	2.4319108392003183E-5
42-43	0.015935964313445513	5.732361263829322E-5	0.0	6.94831668342948E-5	2.779326673371792E-5
44-45	0.01716755344558339	6.079777098000795E-5	1.7370791708573702E-6	6.94831668342948E-5	3.126742507543266E-5
46-47	0.018477311140409847	7.122024600515217E-5	3.4741583417147405E-6	7.122024600515217E-5	3.300450424629003E-5
48-49	0.02004068239418148	7.469440434686692E-5	3.4741583417147405E-6	8.337980020115377E-5	3.47415834171474E-5
50-51	0.021821188544310283	7.643148351772429E-5	3.4741583417147405E-6	8.511687937201114E-5	3.47415834171474E-5
52-53	0.024053335278862005	7.990564185943903E-5	3.4741583417147405E-6	9.032811688458325E-5	3.47415834171474E-5
54-55	0.026822239477208652	8.511687937201114E-5	3.4741583417147405E-6	9.380227522629799E-5	3.47415834171474E-5
56-57	0.030470105736009133	8.685395854286851E-5	3.4741583417147405E-6	9.727643356801273E-5	3.47415834171474E-5
58-59	0.034833648613202844	8.685395854286851E-5	3.4741583417147405E-6	9.727643356801273E-5	3.647866258800477E-5
60-61	0.0401855895386144	8.685395854286851E-5	3.4741583417147405E-6	9.727643356801273E-5	4.1689900100576884E-5
62-63	0.04669442519181697	8.859103771372588E-5	3.4741583417147405E-6	9.727643356801273E-5	4.1689900100576884E-5
64-65	0.05534855362102839	9.727643356801273E-5	3.4741583417147405E-6	9.727643356801273E-5	4.1689900100576884E-5
66-67	0.06600032309672578	9.727643356801273E-5	3.4741583417147405E-6	9.727643356801273E-5	4.1689900100576884E-5
68-69	0.07911874499504064	9.727643356801273E-5	3.4741583417147405E-6	9.727643356801273E-5	4.1689900100576884E-5
70-71	0.09551503528876336	9.727643356801273E-5	3.4741583417147405E-6	9.727643356801273E-5	4.1689900100576884E-5
72-73	0.11588228856706603	9.727643356801273E-5	3.4741583417147405E-6	9.727643356801273E-5	4.1689900100576884E-5
74-75	0.14128012312417162	1.0422475025144221E-4	3.4741583417147405E-6	9.727643356801273E-5	4.5164058442291625E-5
76-77	0.1728862786379215	1.1464722527658644E-4	3.4741583417147405E-6	9.727643356801273E-5	4.5164058442291625E-5
78-79	0.21089704505462245	1.1464722527658644E-4	3.4741583417147405E-6	1.0422475025144221E-4	4.8638216784006366E-5
80-81	0.25675940932359875	1.1464722527658644E-4	3.4741583417147405E-6	1.0422475025144221E-4	5.211237512572111E-5
82-83	0.3133795048976947	1.1812138361830118E-4	3.4741583417147405E-6	1.0769890859315695E-4	5.384945429657848E-5
84-85	0.38056451598894525	1.2333262113087327E-4	3.4741583417147405E-6	1.0769890859315695E-4	5.906069180915059E-5
86-87	0.46110071758740545	1.2506970030173065E-4	3.4741583417147405E-6	1.1464722527658644E-4	6.253485015086532E-5
88	0.5307714889721529	1.2854385864344539E-4	3.4741583417147405E-6	1.1812138361830118E-4	6.253485015086532E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATCTCGA	18735	0.0	39.62995	1
TATCTCG	9275	0.0	33.697147	2
TCTCGAG	22635	0.0	32.68208	2
GATCCGG	32240	0.0	27.383919	1
CTCGAGG	27010	0.0	27.127184	3
ATCCGGC	38245	0.0	23.115303	2
TCGAGGG	31745	0.0	22.829279	4
ATATCTC	15930	0.0	19.932655	1
TCCGGCG	45495	0.0	19.441917	3
TATGGAT	20685	0.0	17.381664	2
CGGCGCG	51690	0.0	17.020899	5
CGAGGGC	43420	0.0	16.777414	5
GCGCCGT	51910	0.0	16.713713	9
GGATCCG	21675	0.0	16.631128	2
CCGGCGC	54575	0.0	16.241686	4
AGGGCGC	48075	0.0	15.006539	7
CGTCGAC	49555	0.0	14.989918	12-13
ATATGGA	25105	0.0	14.712175	1
ATGGATC	25805	0.0	14.276571	1
GCCGTCG	52550	0.0	14.207142	10-11
>>END_MODULE