FastQCFastQC Report
Wed 25 May 2016
SRR491077_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR491077_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31140174
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1838410.5903660011662106No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCTCG115900.039.1309432
ATCTCGA210550.036.5377161
TCTCGAG243750.031.3125042
ATATCTC179900.026.1866071
CTCGAGG299800.025.0507183
GATCCGG304900.024.2733231
TCGAGGG339500.022.024474
ATCCGGC349100.020.9611532
TCCGGCG433100.016.9933343
GGATCCG214050.016.4892045
GCGCCGT449550.015.9117769
CGGCGCG461100.015.79834655
TATGGAT237550.015.7681122
CGAGGGC478350.015.7297065
AGGGCGC514300.014.5481767
ATGGATC253050.014.5050673
CCGGCGC509850.014.4260344
CGTCGAC425500.014.0497812-13
GGGCGCG556200.013.4353248
TCGACTT457900.013.18892114-15
Produced by FastQC (version 0.11.5)