##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR491077_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	31140174
Sequences flagged as poor quality	0
Sequence length	100
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.26275081186123	31.0	30.0	34.0	26.0	34.0
2	30.57550580802792	31.0	31.0	34.0	27.0	34.0
3	30.680367360824636	31.0	31.0	34.0	27.0	34.0
4	34.147820657649504	37.0	35.0	37.0	30.0	37.0
5	33.743783255674806	37.0	35.0	37.0	28.0	37.0
6	33.64408930406105	37.0	35.0	37.0	28.0	37.0
7	33.56484135252423	37.0	35.0	37.0	28.0	37.0
8	33.51728673706191	37.0	35.0	37.0	28.0	37.0
9	34.93523716341469	39.0	35.0	39.0	28.0	39.0
10-11	34.85622304165673	39.0	35.0	39.0	27.0	39.0
12-13	34.79798030351404	39.0	35.0	39.0	27.0	39.0
14-15	35.87736317722566	40.0	36.0	41.0	27.0	41.0
16-17	35.80386292960341	39.5	36.0	41.0	27.0	41.0
18-19	35.75145100345297	39.5	36.0	41.0	27.0	41.0
20-21	35.68774323483228	39.5	35.0	41.0	27.0	41.0
22-23	35.56178215317615	39.0	35.0	41.0	26.5	41.0
24-25	35.438412354407525	39.0	35.0	41.0	26.0	41.0
26-27	35.27610065698413	39.0	35.0	41.0	25.0	41.0
28-29	35.06018209789065	39.0	34.5	41.0	25.0	41.0
30-31	34.885938883963846	39.0	34.0	41.0	24.0	41.0
32-33	34.66122191545879	38.5	34.0	41.0	24.0	41.0
34-35	34.504680497289456	38.0	34.0	40.5	23.0	41.0
36-37	34.32164688610924	38.0	33.5	40.0	22.0	41.0
38-39	34.12936974918637	38.0	33.0	40.0	20.5	41.0
40-41	33.89494324919315	38.0	33.0	40.0	18.5	41.0
42-43	33.59210712823891	38.0	33.0	40.0	15.5	41.0
44-45	33.294077836559296	38.0	33.0	40.0	13.5	41.0
46-47	33.17563405393946	38.0	33.0	40.0	9.0	41.0
48-49	33.01462003712632	38.0	32.0	40.0	8.0	41.0
50-51	32.77566348858552	38.0	32.0	40.0	6.5	41.0
52-53	32.54948288021769	37.5	32.0	40.0	2.0	41.0
54-55	32.30345984900406	37.0	31.0	40.0	2.0	41.0
56-57	32.01892222888671	37.0	31.0	40.0	2.0	41.0
58-59	31.72468530522662	36.0	31.0	40.0	2.0	41.0
60-61	31.42501249350758	36.0	30.0	40.0	2.0	41.0
62-63	31.17336303258935	35.5	30.0	39.0	2.0	41.0
64-65	31.04508566650912	35.0	30.0	39.0	2.0	41.0
66-67	30.8306735858316	35.0	30.0	39.0	2.0	41.0
68-69	30.515444936177943	35.0	30.0	38.5	2.0	40.5
70-71	30.15347884697112	35.0	29.0	37.5	2.0	40.0
72-73	29.7735628098931	35.0	29.0	37.0	2.0	39.5
74-75	29.410638167917753	35.0	29.0	36.5	2.0	39.0
76-77	28.59199834593089	34.0	27.5	36.0	2.0	39.0
78-79	28.732400740599587	34.0	29.0	36.0	2.0	38.5
80-81	28.44701468270537	34.0	28.0	35.0	2.0	37.0
82-83	28.196072780453957	34.0	28.0	35.0	2.0	37.0
84-85	27.906136683757772	34.0	27.0	35.0	2.0	36.5
86-87	27.59405397028289	34.0	27.0	35.0	2.0	36.0
88-89	27.337604375621023	34.0	26.0	35.0	2.0	36.0
90-91	27.012046608988122	34.0	26.0	35.0	2.0	35.5
92-93	26.78445216780099	34.0	25.0	35.0	2.0	35.0
94-95	26.404578038003258	34.0	24.5	35.0	2.0	35.0
96-97	26.15808676920045	34.0	24.0	35.0	2.0	35.0
98-99	25.807166299070776	33.5	21.5	35.0	2.0	35.0
100	25.062100841183483	33.0	18.0	35.0	2.0	35.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	0.7100308729283356
1101	2	0.7087701185045567
1101	3	0.6949980668444553
1101	4	0.778242903213247
1101	5	0.9711963930590031
1101	6	0.9872034895881114
1101	7	0.943336307325005
1101	8	1.0369622771506428
1101	9	1.0760182812309296
1101	10-11	1.1473445458560292
1101	12-13	1.1357767516853414
1101	14-15	1.2516645483207185
1101	16-17	1.2563710736814002
1101	18-19	1.2328632731435718
1101	20-21	1.2755748747603306
1101	22-23	1.2844142900503144
1101	24-25	1.322768146150331
1101	26-27	1.3691069749403084
1101	28-29	1.4149787193056724
1101	30-31	1.4466243222722497
1101	32-33	1.3533753064679104
1101	34-35	1.4144633756614837
1101	36-37	1.3741688658138216
1101	38-39	1.3890282676900654
1101	40-41	1.3817986886846825
1101	42-43	1.303604235900501
1101	44-45	1.4343310493700372
1101	46-47	1.2446122757232416
1101	48-49	1.3160183374210916
1101	50-51	1.2071908772444644
1101	52-53	1.335190377099174
1101	54-55	1.260971981617054
1101	56-57	1.26404947594326
1101	58-59	1.1655113624558489
1101	60-61	1.200239839291175
1101	62-63	1.2495454595032243
1101	64-65	1.4023837073111594
1101	66-67	1.3015131122901487
1101	68-69	1.259475769110736
1101	70-71	1.1838199087828976
1101	72-73	1.228214036710778
1101	74-75	1.2325410473875706
1101	76-77	1.1181888831104594
1101	78-79	1.230277648916644
1101	80-81	1.1974484690083962
1101	82-83	1.2089964901761618
1101	84-85	1.1425906369650498
1101	86-87	1.095353032152513
1101	88-89	1.1550229201319944
1101	90-91	1.2429895650379166
1101	92-93	1.256338408799568
1101	94-95	1.3507185478738215
1101	96-97	1.2364991844050195
1101	98-99	1.1944363856920575
1101	100	1.1724636686746628
1102	1	0.40619202150749345
1102	2	0.4504281033525608
1102	3	0.4479621989644187
1102	4	0.49146101753461124
1102	5	0.47578051609855976
1102	6	0.6126747093460239
1102	7	0.6167248713465199
1102	8	0.65553631274895
1102	9	0.6186905191457797
1102	10-11	0.7329804961034796
1102	12-13	0.7479431233497564
1102	14-15	0.8104557320302561
1102	16-17	0.6356763866106334
1102	18-19	0.7297933464562547
1102	20-21	0.7228581470589077
1102	22-23	0.7423771408262994
1102	24-25	0.7873185764789383
1102	26-27	0.7461506414927541
1102	28-29	0.7734001993735262
1102	30-31	0.7643258548341194
1102	32-33	0.7685444296324775
1102	34-35	0.7585313226598345
1102	36-37	0.7848692059960953
1102	38-39	0.7801382579584555
1102	40-41	0.7696360256308594
1102	42-43	0.7309963010673286
1102	44-45	0.7417009063255122
1102	46-47	0.7699203972782414
1102	48-49	0.7395169365171839
1102	50-51	0.6691252128757199
1102	52-53	0.6455613603624286
1102	54-55	0.631400102688275
1102	56-57	0.7436737124997563
1102	58-59	0.7247362482802835
1102	60-61	0.6631776887589105
1102	62-63	0.6684536747320635
1102	64-65	0.7567448312740197
1102	66-67	0.8179224276105117
1102	68-69	0.8380081180297374
1102	70-71	0.8241149365226441
1102	72-73	0.7537141814825041
1102	74-75	0.8358256993363504
1102	76-77	0.7900246342986144
1102	78-79	0.6852704988034368
1102	80-81	0.8518862495273929
1102	82-83	0.7868223468178854
1102	84-85	0.7927113921701583
1102	86-87	0.7431661337113802
1102	88-89	0.6183858317621436
1102	90-91	0.6255950949274265
1102	92-93	0.7316404700752379
1102	94-95	0.8776811127216817
1102	96-97	0.7075600409635356
1102	98-99	0.629294539497316
1102	100	0.5604827023003409
1103	1	0.18547651011717647
1103	2	0.13633614017379259
1103	3	0.16163503964651582
1103	4	0.24218086904102165
1103	5	0.3239264858814792
1103	6	0.2599406641505908
1103	7	0.24372824842177465
1103	8	0.3402212503031734
1103	9	0.39319952093961064
1103	10-11	0.3957930076825207
1103	12-13	0.31784695076661507
1103	14-15	0.3256020170365872
1103	16-17	0.3011894436912357
1103	18-19	0.37669782947876485
1103	20-21	0.3787535747964341
1103	22-23	0.3279471568864736
1103	24-25	0.3411025124741158
1103	26-27	0.27662566408701394
1103	28-29	0.3593362569200025
1103	30-31	0.35828784494187715
1103	32-33	0.34987516618439685
1103	34-35	0.3557286498419643
1103	36-37	0.39020969510921333
1103	38-39	0.41433883333353805
1103	40-41	0.37372667886891975
1103	42-43	0.330527318147368
1103	44-45	0.4097366302950505
1103	46-47	0.309343537691106
1103	48-49	0.34614471619780574
1103	50-51	0.381407226262759
1103	52-53	0.38311998173845296
1103	54-55	0.21895631465339704
1103	56-57	0.2686803469091359
1103	58-59	0.3538363747502409
1103	60-61	0.30836951724890227
1103	62-63	0.45136860277434465
1103	64-65	0.47260777205261206
1103	66-67	0.37291528493660664
1103	68-69	0.39560297809864053
1103	70-71	0.413058562842874
1103	72-73	0.38231600916067165
1103	74-75	0.5058117995178115
1103	76-77	0.47343879436765235
1103	78-79	0.44969342730462003
1103	80-81	0.5068580577433899
1103	82-83	0.5213435161955857
1103	84-85	0.4134379091447613
1103	86-87	0.5347036701302628
1103	88-89	0.5468453364031483
1103	90-91	0.3860533286747696
1103	92-93	0.4730382496325056
1103	94-95	0.6435335623275655
1103	96-97	0.5336542489532619
1103	98-99	0.5164603636613379
1103	100	0.574101296825436
1104	1	-0.16188082938464277
1104	2	-0.09571671100930601
1104	3	-0.09147934010747605
1104	4	-0.06257273942172503
1104	5	-0.05973451496898008
1104	6	-0.06832868903990885
1104	7	-0.0316685701499253
1104	8	-0.06626885569887264
1104	9	-0.13547786097096548
1104	10-11	-0.21795426593831024
1104	12-13	-0.08968800674377064
1104	14-15	-0.16972643379645547
1104	16-17	-0.18534242476410157
1104	18-19	-0.2583478555956731
1104	20-21	-0.2285090882488774
1104	22-23	-0.21866426338866773
1104	24-25	-0.219289996835478
1104	26-27	-0.16640986384938117
1104	28-29	-0.3505457487607515
1104	30-31	-0.2196706565745714
1104	32-33	-0.3227756218964615
1104	34-35	-0.19301100314459063
1104	36-37	-0.3786474310844312
1104	38-39	-0.40453869835986467
1104	40-41	-0.31858274505202644
1104	42-43	-0.20345642857895285
1104	44-45	-0.3307926165567423
1104	46-47	-0.2567602733150949
1104	48-49	-0.25247005539318224
1104	50-51	-0.17758015881146605
1104	52-53	-0.17425143862312353
1104	54-55	-0.2207507795372834
1104	56-57	-0.20300367471055836
1104	58-59	-0.3740665210578271
1104	60-61	-0.3811887902995039
1104	62-63	-0.1620877832298433
1104	64-65	-0.1324291135954745
1104	66-67	-0.23914832998305258
1104	68-69	-0.24692670456379773
1104	70-71	-0.2647831998007497
1104	72-73	-0.2101252848763906
1104	74-75	-0.2551498723637877
1104	76-77	-0.2357145978782249
1104	78-79	-0.266235462737324
1104	80-81	-0.24343282789285325
1104	82-83	-0.2222600424797676
1104	84-85	-0.20712441345254362
1104	86-87	-0.14909140115631914
1104	88-89	-0.08952431529607097
1104	90-91	-0.07430916923540565
1104	92-93	-0.06011806743009984
1104	94-95	0.058748629867544366
1104	96-97	-0.017363085497706265
1104	98-99	-0.0340016975445252
1104	100	-0.014934418919619219
1105	1	-0.49837692971074077
1105	2	-0.4784233295122533
1105	3	-0.4185367243816991
1105	4	-0.373178125330881
1105	5	-0.541378736581045
1105	6	-0.5816076705840487
1105	7	-0.6206299503187864
1105	8	-0.6492755435504876
1105	9	-0.6251692282959169
1105	10-11	-0.5812117175453082
1105	12-13	-0.6060874482942751
1105	14-15	-0.7330899382809903
1105	16-17	-0.596208793929371
1105	18-19	-0.639233884033743
1105	20-21	-0.6743623128015415
1105	22-23	-0.6991760051737756
1105	24-25	-0.5668101498007516
1105	26-27	-0.5634819456820068
1105	28-29	-0.5941043134641433
1105	30-31	-0.6142677183102094
1105	32-33	-0.69985902028683
1105	34-35	-0.6336302078754414
1105	36-37	-0.6403111987568977
1105	38-39	-0.5999780001421868
1105	40-41	-0.5502479483304654
1105	42-43	-0.6485620661116727
1105	44-45	-0.4941758050213352
1105	46-47	-0.6564750946939881
1105	48-49	-0.6398715505370021
1105	50-51	-0.5409782990021412
1105	52-53	-0.5739114472444058
1105	54-55	-0.5884572618996913
1105	56-57	-0.5027522531161637
1105	58-59	-0.589767800429474
1105	60-61	-0.5660604913213625
1105	62-63	-0.6128792144777364
1105	64-65	-0.7092837638719232
1105	66-67	-0.7302797163685248
1105	68-69	-0.7638841276009316
1105	70-71	-0.6119119065554521
1105	72-73	-0.6379009308440793
1105	74-75	-0.6213538869954895
1105	76-77	-0.5830678110584593
1105	78-79	-0.5520677954246054
1105	80-81	-0.4829542812177827
1105	82-83	-0.4723609909192419
1105	84-85	-0.5852096893209016
1105	86-87	-0.4593369899244202
1105	88-89	-0.4973789940578257
1105	90-91	-0.4714014059967937
1105	92-93	-0.4483822957618422
1105	94-95	-0.3403261430266298
1105	96-97	-0.4893681679519446
1105	98-99	-0.5014507737989611
1105	100	-0.3783482268530811
1106	1	-0.777727256814277
1106	2	-0.709079098520025
1106	3	-0.6789767762815977
1106	4	-0.6615566805481237
1106	5	-0.6852369884912193
1106	6	-0.7237783028984381
1106	7	-0.7068473444089278
1106	8	-0.725552393157308
1106	9	-0.9416178102165773
1106	10-11	-1.0062625455082213
1106	12-13	-0.9735695146815146
1106	14-15	-1.1882584639195528
1106	16-17	-1.1934961005755653
1106	18-19	-1.1073421573810833
1106	20-21	-1.1787245259757029
1106	22-23	-1.1530678452904866
1106	24-25	-1.1186931962419848
1106	26-27	-1.189840151094515
1106	28-29	-1.2261525322484488
1106	30-31	-1.1890543444779027
1106	32-33	-1.112686157544772
1106	34-35	-1.104320860349084
1106	36-37	-1.1368312682643804
1106	38-39	-1.1579496432624907
1106	40-41	-1.2166852467465645
1106	42-43	-1.2836725578298385
1106	44-45	-1.1988285853018184
1106	46-47	-1.157842450160885
1106	48-49	-1.1251570040804921
1106	50-51	-1.1491363497723626
1106	52-53	-1.201338640738939
1106	54-55	-1.099219547801006
1106	56-57	-1.05478451428462
1106	58-59	-1.127167991362363
1106	60-61	-1.087953551861144
1106	62-63	-1.1349294727561308
1106	64-65	-1.0835259532832922
1106	66-67	-1.126322767196104
1106	68-69	-1.2132909038427897
1106	70-71	-1.164360356770299
1106	72-73	-1.1014948679794365
1106	74-75	-1.071400627255663
1106	76-77	-1.053743685359148
1106	78-79	-1.064302627133177
1106	80-81	-0.9371632284426994
1106	82-83	-0.9982243235264612
1106	84-85	-0.9675919928623564
1106	86-87	-0.9322968134651894
1106	88-89	-0.9206371094528798
1106	90-91	-0.9251197867929974
1106	92-93	-0.9040784712178969
1106	94-95	-0.7624941048292442
1106	96-97	-0.8712895159238805
1106	98-99	-0.9353422628415089
1106	100	-0.9487637646524973
1107	1	-1.1876357132181568
1107	2	-1.1441958402800587
1107	3	-1.133544628800223
1107	4	-1.166180964385255
1107	5	-1.313025451856113
1107	6	-1.3685785955416847
1107	7	-1.4050769083837622
1107	8	-1.4835800093225728
1107	9	-1.6153170315449188
1107	10-11	-1.6810423790820224
1107	12-13	-1.7222581008812554
1107	14-15	-1.9560534345199727
1107	16-17	-1.9724811644253393
1107	18-19	-1.9781627080969315
1107	20-21	-2.0331754170905896
1107	22-23	-2.2465490054028905
1107	24-25	-2.1403835368778417
1107	26-27	-2.1129699675657605
1107	28-29	-2.1205729787947334
1107	30-31	-2.026245972968013
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2308	78-79	-2.465288842990912
2308	80-81	-2.6453817758366505
2308	82-83	-2.4503336163346603
2308	84-85	-2.327526622841763
2308	86-87	-2.5582602369004057
2308	88-89	-2.6820686429623883
2308	90-91	-2.704613209549045
2308	92-93	-2.517512572386149
2308	94-95	-2.220630069019027
2308	96-97	-2.3992207570950015
2308	98-99	-2.6247422663719178
2308	100	-2.9651806682821658
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	882808.0
3	248966.0
4	133148.0
5	107540.0
6	103844.0
7	111039.0
8	122204.0
9	131869.0
10	141943.0
11	144775.0
12	152467.0
13	174956.0
14	219313.0
15	274079.0
16	309395.0
17	283537.0
18	241253.0
19	203032.0
20	191034.0
21	196975.0
22	214527.0
23	246278.0
24	288516.0
25	343738.0
26	410344.0
27	486942.0
28	572660.0
29	673135.0
30	796381.0
31	947670.0
32	1147233.0
33	1421074.0
34	1828600.0
35	2493317.0
36	3548695.0
37	5060673.0
38	4949186.0
39	1333891.0
40	3137.0
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	21.11360555886984	29.013541961772017	27.85845488441452	22.014397594943624
2	23.458297943112726	28.538824831550365	27.4663811235572	20.53649610177971
3	23.041859046773467	27.29326432151599	27.06109798872672	22.60377864298382
4	23.868527702027965	26.929048559389084	26.784328672459807	22.418095066123144
5	24.21932155899787	26.927130555984974	26.40566884728074	22.447879037736417
6	24.187873641805023	26.71137472245862	25.96320398834282	23.137547647393536
7	24.081021004312053	27.079352324467077	26.078028806781674	22.7615978644392
8	23.567340037712615	26.82942408682303	26.4835484850147	23.11968739044966
9	23.502584536188984	26.612066251555273	26.538266862124786	23.34708235013096
10-11	23.546458042111652	26.811893958097986	26.488470897014594	23.153177102775764
12-13	23.19793079822781	26.854190084452885	26.655039144695337	23.292839972623973
14-15	22.96787587643131	27.01043789262086	27.062121630025466	22.95956460092237
16-17	22.8108961571099	26.96686036847618	27.24440860402902	22.977834870384896
18-19	22.504580030506617	26.921287412748125	27.793131385687776	22.781001171057486
20-21	22.225654738193757	26.740755918265453	28.49149374105243	22.54209560248836
22-23	22.20930240026307	26.704784405354925	28.795485023725178	22.290428170656828
24-25	22.12355536648742	26.5571380270911	29.1429218463287	22.17638476009278
26-27	22.157813547929624	26.610341252630842	29.24881434047542	21.983030858964117
28-29	22.184930601297	26.68567840868617	29.208756422628696	21.920634567388134
30-31	22.17785572969961	26.612779797759746	29.163307106822632	22.046057365718013
32-33	22.214729121935868	26.68250461865335	29.058741736869926	22.04402452254085
34-35	22.266502089150087	26.767579098428463	29.051837164983258	21.914081647438195
36-37	22.26807432375746	26.730803588926026	28.96105710089009	22.040064986426426
38-39	22.279181557570997	26.787372152390265	28.872738076736752	22.060708213301982
40-41	22.3636849364215	26.915073430852352	28.689810252945975	22.031431379780173
42-43	22.4518620773992	26.978747914405904	28.346198848963848	22.223191159231046
44-45	22.531975913964487	27.143117647416787	28.02305167849634	22.30185476012238
46-47	22.66934653414856	27.3519491605951	27.666964235853214	22.311740069403125
48-49	22.749539093838923	27.37935053372083	27.340275408661885	22.530834963778357
50-51	22.7471919791431	27.50226358120625	27.161548647607574	22.588995792043082
52-53	22.851204898931364	27.60784592669474	27.009188627338553	22.531760547035347
54-55	22.858696824662143	27.568457985410046	26.928190611794665	22.644654578133146
56-57	22.822121217745842	27.61866422111635	26.94001983423685	22.619194726900954
58-59	22.883080828297313	27.74306859137029	26.851137073075158	22.522713507257233
60-61	22.844060820261785	27.730602066796035	26.802359518438017	22.622977594504164
62-63	22.799676931477162	27.79772077475688	26.82968732674092	22.57291496702504
64-65	22.83004663994511	27.937368546769136	26.76655126517844	22.466033548107315
66-67	22.813358711321932	27.95122764558675	26.66493595027329	22.570477692818027
68-69	22.793089513474172	28.077776515382386	26.593505144846386	22.535628826297057
70-71	22.848770843180056	28.20167578457949	26.52450391303066	22.42504945920979
72-73	22.835526200330207	28.23652703393807	26.396149459689898	22.531797306041824
74-75	22.807151741856707	28.36224005514165	26.37670606851692	22.45390213448472
76-77	22.74090243829988	28.56116784054991	26.228706121882077	22.469223599268133
78-79	22.735425559630453	28.6062766660449	26.145414699230905	22.51288307509374
80-81	22.681826816928773	28.699549322562547	26.101009413841496	22.517614446667185
82-83	22.706296827583035	28.861491399079753	26.013741073955497	22.418470699381718
84-85	22.686310132930554	28.87746285949025	25.914772253236208	22.52145475434299
86-87	22.654547193359186	29.02587555978686	25.828808585881504	22.49076866097245
88-89	22.747020880431208	29.13740077221771	25.690538304271083	22.42504004308
90-91	22.70065962602705	29.15441513783012	25.604985286925587	22.539939949217246
92-93	22.709865480903506	29.27558967831653	25.550906782165278	22.463638058614684
94-95	22.759345806172373	29.400910531829293	25.430552383036986	22.409191278961348
96-97	22.702943068241698	29.483181639119614	25.277808651382394	22.536066641256287
98-99	22.71781789049725	29.580455833733986	25.19542639274658	22.506299883022184
100	22.78791393340071	29.727835047838468	24.84366305336914	22.640587965391674
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	74939.0
1	44406.5
2	10953.5
3	7576.0
4	7251.0
5	7496.0
6	7829.5
7	8352.0
8	9149.5
9	10168.0
10	11489.0
11	13083.5
12	15272.0
13	19693.5
14	29889.0
15	40585.5
16	56524.5
17	55130.5
18	42140.5
19	46158.5
20	54589.0
21	65133.0
22	79080.0
23	98280.0
24	122127.0
25	148977.5
26	179635.5
27	217360.0
28	261168.5
29	306277.5
30	352448.0
31	403279.0
32	458027.0
33	515562.5
34	580117.5
35	651792.0
36	727461.0
37	812761.0
38	888866.5
39	942338.5
40	986807.0
41	1038231.0
42	1117378.5
43	1188229.0
44	1240153.5
45	1270663.0
46	1254284.0
47	1208858.5
48	1168120.0
49	1149955.0
50	1113015.5
51	1051992.0
52	971512.5
53	878417.5
54	812321.5
55	761128.0
56	697924.5
57	631199.0
58	579044.0
59	533835.5
60	482520.5
61	433861.5
62	386241.5
63	334685.0
64	287957.0
65	244903.5
66	203722.0
67	168311.5
68	138328.5
69	112530.5
70	88768.0
71	68013.0
72	52405.5
73	40697.5
74	31220.0
75	22414.5
76	15117.0
77	10484.0
78	7710.5
79	5021.5
80	2711.0
81	1419.0
82	793.5
83	454.5
84	284.5
85	187.5
86	124.0
87	82.0
88	65.0
89	56.5
90	36.5
91	24.0
92	22.5
93	16.0
94	7.0
95	3.0
96	1.0
97	0.5
98	2.0
99	2.0
100	0.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.23856963676567766
2	0.0014482899164275703
3	0.0
4	5.4591859377535916E-5
5	5.138057353179851E-5
6	7.161167435994416E-4
7	0.0038117962988903015
8	0.004887577057212333
9	0.005475242366982278
10-11	0.00397557187702291
12-13	0.0063679798320972775
14-15	0.005627778444654805
16-17	0.0028965798328551406
18-19	0.001189781405845709
20-21	2.3924079550743683E-4
22-23	8.156666048173013E-4
24-25	0.0010035268267929395
26-27	0.0020825188709607083
28-29	0.0015125156333423186
30-31	5.84454023924208E-4
32-33	0.0013342892689038924
34-35	7.642860312855027E-4
36-37	0.0014145714150473276
38-39	7.048772431393608E-4
40-41	0.0012909369099864375
42-43	0.002137110730338244
44-45	1.7822636443842608E-4
46-47	2.472690101217803E-4
48-49	5.08988806549379E-4
50-51	0.001152851618619729
52-53	0.0010324283994045762
54-55	0.0016923476407036133
56-57	2.569028676589925E-4
58-59	9.63385753721222E-6
60-61	2.92227011962104E-4
62-63	9.954986121785962E-5
64-65	0.002479112672909278
66-67	0.0027392268264140075
68-69	2.569028676589925E-4
70-71	0.0011945983346143153
72-73	7.46623959133947E-4
74-75	3.853543014884888E-5
76-77	0.002084124513883577
78-79	0.010269692134668227
80-81	0.02154612238197513
82-83	0.0017373056425439369
84-85	0.0014643463456562576
86-87	0.0027841848282543318
88-89	0.00288534033239506
90-91	0.002771339684871382
92-93	1.9267715074424438E-4
94-95	0.0011271613318538298
96-97	0.0021355050874153755
98-99	0.0016329388525574712
100	0.0012267111930716894
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	3.1140174E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	47.47300704434923
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.71227256656016	36.89235262984698
2	12.17737501842311	11.561932200625654
3	3.9911712049037007	5.684186961767916
4	1.840043441733789	3.4940958108534703
5	0.9772345257726044	2.3196130762992064
6	0.6397481416147333	1.8222460820091337
7	0.4219077719230119	1.4020461439996783
8	0.2958536728032457	1.123605079446806
9	0.2220067795783849	0.9485396469736158
>10	1.443940452648779	13.740562409057306
>50	0.14100600697162255	4.633345269654668
>100	0.13094998074697609	12.921086676311239
>500	0.006120616710057893	1.7732204004621193
>1k	2.3373640020319503E-4	0.17864934661457854
>5k	4.082496291669814E-5	0.15531157161647832
>10k+	9.525824680544423E-5	1.3492066944612393
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	183841	0.5903660011662106	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	6.422571691474813E-6	0.0	0.0	0.0
2	0.0	1.2845143382949626E-5	0.0	0.0	0.0
3	3.2112858457374064E-6	1.926771507442444E-5	0.0	3.2112858457374064E-6	3.2112858457374064E-6
4	3.2112858457374064E-6	2.569028676589925E-5	0.0	3.2112858457374064E-6	3.2112858457374064E-6
5	3.2112858457374064E-6	2.8901572611636658E-5	0.0	6.422571691474813E-6	3.2112858457374064E-6
6	3.2112858457374064E-6	2.8901572611636658E-5	0.0	6.422571691474813E-6	3.2112858457374064E-6
7	3.2112858457374064E-6	3.211285845737407E-5	0.0	6.422571691474813E-6	3.2112858457374064E-6
8	3.2112858457374064E-6	3.853543014884888E-5	0.0	6.422571691474813E-6	3.2112858457374064E-6
9	3.2112858457374064E-6	5.13805735317985E-5	0.0	6.422571691474813E-6	3.2112858457374064E-6
10-11	2.5690286765899255E-5	5.7803145223273316E-5	0.0	6.422571691474813E-6	3.2112858457374064E-6
12-13	9.95498612178596E-5	6.904264568335424E-5	0.0	9.63385753721222E-6	3.2112858457374064E-6
14-15	2.4084643843030548E-4	8.349343198917256E-5	0.0	9.63385753721222E-6	3.2112858457374064E-6
16-17	5.073831636265103E-4	9.79442182949909E-5	0.0	9.63385753721222E-6	3.2112858457374064E-6
18-19	9.938929692557275E-4	1.1400064752367794E-4	0.0	1.2845143382949626E-5	3.2112858457374064E-6
20-21	0.0016939532836264819	1.1560629044654663E-4	0.0	1.445078630581833E-5	3.2112858457374064E-6
22-23	0.0027504663268740886	1.2202886213802145E-4	0.0	1.926771507442444E-5	3.2112858457374064E-6
24-25	0.0042035731720702655	1.2845143382949627E-4	0.0	1.926771507442444E-5	3.2112858457374064E-6
26-27	0.00590555467031109	1.3487400552097107E-4	0.0	2.0873357997293144E-5	3.2112858457374064E-6
28-29	0.007978439683734587	1.3647964844383978E-4	0.0	2.2479000920161848E-5	3.2112858457374064E-6
30-31	0.009516645603842805	1.4290222013531458E-4	0.0	3.050721553450536E-5	3.2112858457374064E-6
32-33	0.010255241348362407	1.6538122105547642E-4	0.0	3.5324144303111475E-5	3.2112858457374064E-6
34-35	0.011050034595182417	1.7822636443842608E-4	0.0	3.5324144303111475E-5	3.2112858457374064E-6
36-37	0.012040716278592406	1.7983200736129478E-4	1.6056429228687032E-6	3.5324144303111475E-5	3.2112858457374064E-6
38-39	0.012949510172936092	1.814376502841635E-4	3.2112858457374064E-6	3.5324144303111475E-5	3.2112858457374064E-6
40-41	0.013906473354965839	1.878602219756383E-4	3.2112858457374064E-6	3.5324144303111475E-5	3.2112858457374064E-6
42-43	0.015014366971745245	1.974940795128505E-4	3.2112858457374064E-6	3.6929787225980175E-5	4.816928768606109E-6
44-45	0.016570234964005018	2.0231100828145662E-4	3.2112858457374064E-6	4.335235891745499E-5	6.422571691474813E-6
46-47	0.018468104898835824	2.0552229412719404E-4	3.2112858457374064E-6	4.4958001840323696E-5	6.422571691474813E-6
48-49	0.02076577992146094	2.1515615166440625E-4	3.2112858457374064E-6	4.4958001840323696E-5	6.422571691474813E-6
50-51	0.023429541530500118	2.2157872335588105E-4	3.2112858457374064E-6	4.4958001840323696E-5	6.422571691474813E-6
52-53	0.026788546525141448	2.2639565212448717E-4	3.2112858457374064E-6	4.4958001840323696E-5	6.422571691474813E-6
54-55	0.030927893980296963	2.2800129504735588E-4	3.2112858457374064E-6	4.4958001840323696E-5	6.422571691474813E-6
56-57	0.03628110748514122	2.312125808930933E-4	3.2112858457374064E-6	4.4958001840323696E-5	6.422571691474813E-6
58-59	0.04300554004611535	2.4245208135317419E-4	3.2112858457374064E-6	4.4958001840323696E-5	8.028214614343517E-6
60-61	0.051250516455046136	2.520859388903864E-4	3.2112858457374064E-6	4.6563644763192396E-5	1.2845143382949626E-5
62-63	0.06185418231767106	2.633254393504674E-4	3.2112858457374064E-6	4.8169287686061096E-5	1.2845143382949626E-5
64-65	0.07478442477553272	2.6653672519620473E-4	3.2112858457374064E-6	4.8169287686061096E-5	1.6056429228687034E-5
66-67	0.09111863022987604	2.6814236811907344E-4	3.2112858457374064E-6	5.13805735317985E-5	1.6056429228687034E-5
68-69	0.11180412800519354	2.7295929688767956E-4	3.2112858457374064E-6	5.29862164546672E-5	1.926771507442444E-5
70-71	0.1370528629673039	2.76170582733417E-4	3.2112858457374064E-6	5.459185937753591E-5	2.2479000920161848E-5
72-73	0.16818627924172808	2.793818685791544E-4	3.2112858457374064E-6	5.9408788146142016E-5	2.2479000920161848E-5
74-75	0.20639415823431173	2.8419879734776045E-4	3.2112858457374064E-6	6.422571691474814E-5	2.2479000920161848E-5
76-77	0.25298349328427006	2.890157261163666E-4	3.2112858457374064E-6	6.743700276048554E-5	2.2479000920161848E-5
78-79	0.30935601066326734	2.906213690392353E-4	3.2112858457374064E-6	6.743700276048554E-5	2.2479000920161848E-5
80-81	0.3764847299825621	2.938326548849727E-4	3.2112858457374064E-6	6.743700276048554E-5	2.4084643843030548E-5
82-83	0.457246963359935	3.0186086949931623E-4	3.2112858457374064E-6	6.743700276048554E-5	2.569028676589925E-5
84-85	0.5527570269838569	3.0507215534505364E-4	3.2112858457374064E-6	6.743700276048554E-5	2.7295929688767955E-5
86-87	0.6645948734904308	3.0667779826792235E-4	3.2112858457374064E-6	7.385957445196035E-5	3.050721553450536E-5
88	0.7599764856805231	3.0828344119079106E-4	3.2112858457374064E-6	7.385957445196035E-5	3.211285845737407E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATCTCG	11590	0.0	39.130943	2
ATCTCGA	21055	0.0	36.537716	1
TCTCGAG	24375	0.0	31.312504	2
ATATCTC	17990	0.0	26.186607	1
CTCGAGG	29980	0.0	25.050718	3
GATCCGG	30490	0.0	24.273323	1
TCGAGGG	33950	0.0	22.02447	4
ATCCGGC	34910	0.0	20.961153	2
TCCGGCG	43310	0.0	16.993334	3
GGATCCG	21405	0.0	16.489204	5
GCGCCGT	44955	0.0	15.911776	9
CGGCGCG	46110	0.0	15.7983465	5
TATGGAT	23755	0.0	15.768112	2
CGAGGGC	47835	0.0	15.729706	5
AGGGCGC	51430	0.0	14.548176	7
ATGGATC	25305	0.0	14.505067	3
CCGGCGC	50985	0.0	14.426034	4
CGTCGAC	42550	0.0	14.04978	12-13
GGGCGCG	55620	0.0	13.435324	8
TCGACTT	45790	0.0	13.188921	14-15
>>END_MODULE