FastQCFastQC Report
Wed 25 May 2016
SRR491076_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR491076_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33715762
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCTCGA236950.043.4609341
TCTCGAG289900.035.1966672
TATCTCG129650.029.4356382
CTCGAGG346900.029.115353
TCGAGGG406550.024.7625894
GATCCGG400900.024.3125231
ATATCTC167600.023.5545921
ATCCGGC461650.021.063842
TATGGAT249850.017.4001222
CGAGGGC586350.017.2414865
TCCGGCG575650.016.9332433
AGGGCGC622950.016.0708987
ATGGATC274150.015.6715571
GCGCCGT611350.015.6609269
CGGCGCG633300.015.3621515
GGATCCG282800.014.9739122
GGGCGCG684000.014.6364998
CCGGCGC682500.014.3442284
GAGGGCG716200.014.1286586
CGTCGAC582550.013.83313512-13
Produced by FastQC (version 0.11.5)