FastQCFastQC Report
Wed 25 May 2016
SRR491075_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR491075_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38820522
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2386170.6146671598078974No Hit
GATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT534430.137666876298057No Hit
ATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTT512400.13199204276542184No Hit
ATATGGATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTT420360.10828293344432617No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCTCGA322650.043.388751
TATCTCG143050.037.207422
TCTCGAG386550.036.101832
ATATCTC173400.031.0622041
CTCGAGG470100.029.5659143
TATGGAT212800.029.209952
GATCCGG564600.025.9033371
ATATGGA254100.024.7145351
TCGAGGG579250.023.9067924
ATCCGGC661050.022.1988492
AACGGTA34250.021.74868264-65
ATTCGTG57600.021.2237382
ACGGTAC35700.020.9974266-67
ACGTAAC29900.019.1762814-15
TCCGGCG795600.018.533543
TAACGGT41100.018.18107264-65
CGAGGGC767100.018.1310025
CACGTTA42100.018.0853174-75
GCTTACG45900.017.81599268-69
TTACGTC47250.017.0089470-71
Produced by FastQC (version 0.11.5)