##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR491075_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 38820522 Sequences flagged as poor quality 0 Sequence length 100 %GC 48 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.512749828557173 33.0 31.0 34.0 30.0 34.0 2 31.784577806552935 34.0 31.0 34.0 30.0 34.0 3 31.840194086004306 34.0 31.0 34.0 30.0 34.0 4 35.25927683301116 37.0 35.0 37.0 33.0 37.0 5 35.02473804963262 37.0 35.0 37.0 32.0 37.0 6 34.98332101253043 37.0 35.0 37.0 32.0 37.0 7 34.90180343273076 37.0 35.0 37.0 32.0 37.0 8 34.89315277625582 37.0 35.0 37.0 32.0 37.0 9 36.51340097899765 39.0 37.0 39.0 32.0 39.0 10-11 36.503097150522606 39.0 37.0 39.0 32.0 39.0 12-13 36.4691494359607 39.0 37.0 39.0 32.0 39.0 14-15 37.754372481132535 40.0 38.0 41.0 32.0 41.0 16-17 37.70330119466194 40.0 38.0 41.0 32.0 41.0 18-19 37.65607105952877 40.0 38.0 41.0 32.0 41.0 20-21 37.60879270247834 40.0 38.0 41.0 32.0 41.0 22-23 37.52438872408774 40.0 38.0 41.0 32.0 41.0 24-25 37.44694583189788 40.0 38.0 41.0 32.0 41.0 26-27 37.22859775043726 40.0 37.5 41.0 31.5 41.0 28-29 37.03952646489401 40.0 37.0 41.0 31.0 41.0 30-31 36.86508630667048 40.0 36.5 41.0 31.0 41.0 32-33 36.700570976351116 40.0 36.0 41.0 30.0 41.0 34-35 36.54888723546788 40.0 36.0 41.0 30.0 41.0 36-37 36.36996013345725 40.0 35.0 41.0 30.0 41.0 38-39 36.16398675679837 40.0 35.0 41.0 29.5 41.0 40-41 35.85953063691416 40.0 35.0 41.0 28.5 41.0 42-43 35.50109954729614 39.5 35.0 41.0 26.5 41.0 44-45 35.121614799512486 39.0 35.0 41.0 24.5 41.0 46-47 34.713198034792015 39.0 35.0 41.0 23.0 41.0 48-49 34.360391483143886 39.0 34.0 41.0 20.5 41.0 50-51 34.07157359965433 38.0 34.0 40.0 19.5 41.0 52-53 33.84331416254526 38.0 34.0 40.0 18.0 41.0 54-55 33.67056027737082 38.0 34.0 40.0 15.5 41.0 56-57 33.38818917736346 37.5 33.0 40.0 13.0 41.0 58-59 33.092265670719215 37.0 33.0 40.0 10.0 41.0 60-61 32.74204302301757 36.5 33.0 40.0 7.5 41.0 62-63 32.37155928763657 36.0 32.5 39.0 6.5 41.0 64-65 32.00568696629067 35.0 32.0 39.0 3.5 41.0 66-67 31.713739552497515 35.0 32.0 39.0 2.0 41.0 68-69 31.57270904291292 35.0 32.0 38.0 2.0 40.0 70-71 31.279991675536976 35.0 32.0 37.0 2.0 39.5 72-73 30.929880656937073 35.0 32.0 37.0 2.0 39.0 74-75 30.615494737551444 35.0 32.0 36.0 2.0 39.0 76-77 29.950529851195718 34.5 30.5 35.0 2.0 37.0 78-79 30.033477731185585 35.0 31.0 35.0 2.0 37.0 80-81 29.85740695346652 35.0 31.0 35.0 2.0 37.0 82-83 29.53930785629312 35.0 31.0 35.0 2.0 36.0 84-85 29.357124177773805 35.0 31.0 35.0 2.0 36.0 86-87 29.139083665593162 35.0 31.0 35.0 2.0 36.0 88-89 28.923806794766953 35.0 30.0 35.0 2.0 35.0 90-91 28.736847871339805 35.0 30.0 35.0 2.0 35.0 92-93 28.52841870596176 34.0 30.0 35.0 2.0 35.0 94-95 28.298225227368142 34.0 30.0 35.0 2.0 35.0 96-97 28.04900664396012 34.0 29.0 35.0 2.0 35.0 98-99 27.734993555728074 34.0 29.0 35.0 2.0 35.0 100 27.097997600341387 34.0 27.0 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.41873488885367394 1101 2 0.5055340651849924 1101 3 0.4916654688802389 1101 4 0.3892645717866401 1101 5 0.5246579478360758 1101 6 0.46484370322266244 1101 7 0.5887720548628721 1101 8 0.4984669559226731 1101 9 0.6716890376144988 1101 10-11 0.6589043857568626 1101 12-13 0.6568096922096132 1101 14-15 0.636546067659296 1101 16-17 0.6784590160836217 1101 18-19 0.7686131803091101 1101 20-21 0.7705753289119528 1101 22-23 0.78773931262063 1101 24-25 0.8086542366303391 1101 26-27 0.7808430962685193 1101 28-29 0.7824406660887959 1101 30-31 0.7729262778593196 1101 32-33 0.6924312572888027 1101 34-35 0.7471554808503171 1101 36-37 0.8007424843820203 1101 38-39 0.7121143739375029 1101 40-41 0.8336346570686928 1101 42-43 0.7720341243023512 1101 44-45 0.7759766201648901 1101 46-47 0.7764705473221056 1101 48-49 0.8058545664434575 1101 50-51 0.8313134326176623 1101 52-53 0.829868238686231 1101 54-55 0.7991696340474377 1101 56-57 0.8367004374304869 1101 58-59 0.7517411849130298 1101 60-61 0.7625090050124257 1101 62-63 0.8136547862149186 1101 64-65 0.8078690710056122 1101 66-67 0.7687028524339858 1101 68-69 0.8312932303027303 1101 70-71 0.8020455385319742 1101 72-73 0.7808845958931521 1101 74-75 0.7875131873295622 1101 76-77 0.8131073977749281 1101 78-79 0.7932441714509153 1101 80-81 0.8376526998116063 1101 82-83 0.9026702801444415 1101 84-85 0.8822118941200614 1101 86-87 0.8373091268015322 1101 88-89 0.8188009776336109 1101 90-91 0.8231982921954781 1101 92-93 0.9021114750767687 1101 94-95 0.8533975188967808 1101 96-97 0.8776045247712467 1101 98-99 0.8720541895144187 1101 100 0.8662076483854655 1102 1 0.2361936931423081 1102 2 0.2206591863619174 1102 3 0.31320249375631093 1102 4 0.3087686578419593 1102 5 0.3635508276866801 1102 6 0.38676525076200363 1102 7 0.39765434602907845 1102 8 0.40454762103153996 1102 9 0.4982848599175185 1102 10-11 0.39129041978937096 1102 12-13 0.393850086075318 1102 14-15 0.4365488508001434 1102 16-17 0.44956176593436936 1102 18-19 0.4650903661297363 1102 20-21 0.5030240201911056 1102 22-23 0.42411789021306134 1102 24-25 0.4175146155871232 1102 26-27 0.4867928862922284 1102 28-29 0.49214860725055587 1102 30-31 0.47267588676191963 1102 32-33 0.5034453429155903 1102 34-35 0.522565406940096 1102 36-37 0.464264370365008 1102 38-39 0.46178388002559956 1102 40-41 0.48695648060520114 1102 42-43 0.4950606705851328 1102 44-45 0.45264570359153566 1102 46-47 0.5459838585168981 1102 48-49 0.5266349959865693 1102 50-51 0.4994170966170728 1102 52-53 0.42588604803488295 1102 54-55 0.4197123387249988 1102 56-57 0.5103734537943865 1102 58-59 0.44878811076538483 1102 60-61 0.5413702468236679 1102 62-63 0.5243435956045772 1102 64-65 0.5212752333604556 1102 66-67 0.5372419908064039 1102 68-69 0.6191796737154682 1102 70-71 0.5447393197680874 1102 72-73 0.5401283822568068 1102 74-75 0.5199246619325812 1102 76-77 0.5452864849692425 1102 78-79 0.5520489243159616 1102 80-81 0.5438049489721877 1102 82-83 0.5901501547640642 1102 84-85 0.548059704500254 1102 86-87 0.5030864751353974 1102 88-89 0.5004515490601733 1102 90-91 0.4925083076756209 1102 92-93 0.5477516381092755 1102 94-95 0.5316406439482222 1102 96-97 0.5456975149161174 1102 98-99 0.6007125729891634 1102 100 0.620607738847788 1103 1 0.014179463236953183 1103 2 0.0470696772479009 1103 3 0.05770452420407324 1103 4 0.09640037841749205 1103 5 0.1313042063592178 1103 6 0.14642333720852463 1103 7 0.21807217251351574 1103 8 0.16977131450253324 1103 9 0.13904716806080586 1103 10-11 0.10583831318301407 1103 12-13 0.14739594102259446 1103 14-15 0.03778549669700482 1103 16-17 0.13484943059338406 1103 18-19 0.21492557577074933 1103 20-21 0.17045123526771278 1103 22-23 0.11103949063704732 1103 24-25 0.10478361500150157 1103 26-27 0.12240716355224635 1103 28-29 0.1443734801163501 1103 30-31 0.21433787074349908 1103 32-33 0.19124013235856552 1103 34-35 0.08948988376589995 1103 36-37 0.14799531245168396 1103 38-39 0.10732967214067202 1103 40-41 0.10847865756228003 1103 42-43 0.10770772982550625 1103 44-45 0.18405821013357127 1103 46-47 0.08452778532848271 1103 48-49 0.027248343836632216 1103 50-51 0.02074665813676546 1103 52-53 -0.023975859997442228 1103 54-55 0.022159741151050127 1103 56-57 0.1046637285636578 1103 58-59 0.09197654562142787 1103 60-61 0.14838675356144648 1103 62-63 0.1703293632067897 1103 64-65 0.2169582907037082 1103 66-67 0.09163787384897759 1103 68-69 0.053881895222321674 1103 70-71 0.16584764987143075 1103 72-73 0.12159886136057096 1103 74-75 0.1663844817577811 1103 76-77 0.13946487500558646 1103 78-79 0.15849617224353452 1103 80-81 0.19305486859111198 1103 82-83 0.23977145390951193 1103 84-85 0.20331205504281513 1103 86-87 0.1842567207369754 1103 88-89 0.24798307475488812 1103 90-91 0.25861993565503383 1103 92-93 0.11474944061940917 1103 94-95 0.1550756291380182 1103 96-97 0.02059945139414765 1103 98-99 0.2065261965678431 1103 100 0.24352675897300813 1104 1 -0.17607374182163227 1104 2 -0.17818533529051095 1104 3 -0.09327481162855378 1104 4 -0.1050412110219554 1104 5 -0.08955548416371784 1104 6 -0.06091400509379241 1104 7 -0.03906934234710491 1104 8 -0.045801280923825516 1104 9 -0.08441549058679243 1104 10-11 -0.09983066408899788 1104 12-13 -0.12963914665733967 1104 14-15 -0.19920023549435228 1104 16-17 -0.210281587056798 1104 18-19 -0.10307306322166454 1104 20-21 -0.19244924922198692 1104 22-23 -0.1440679269410623 1104 24-25 -0.08483028534591597 1104 26-27 -0.12387352648536165 1104 28-29 -0.11987516366011164 1104 30-31 -0.12306582168610447 1104 32-33 -0.15868752541911846 1104 34-35 -0.15939258882438168 1104 36-37 -0.178183865790281 1104 38-39 -0.1430939931861488 1104 40-41 -0.11444416513695899 1104 42-43 -0.12045853005380991 1104 44-45 -0.09558717243321979 1104 46-47 -0.13610544751119846 1104 48-49 -0.06605598445099758 1104 50-51 -0.08623424403797486 1104 52-53 -0.09284621248356473 1104 54-55 -0.1269353010454779 1104 56-57 -0.07192729214728644 1104 58-59 -0.02409964864589398 1104 60-61 0.016794948303235913 1104 62-63 0.04994272770472463 1104 64-65 -0.057837897308736785 1104 66-67 0.02147077214457127 1104 68-69 0.019948327675162858 1104 70-71 -0.03530053646597153 1104 72-73 -0.09419171627108369 1104 74-75 -0.06700707042202936 1104 76-77 -0.07889944213484057 1104 78-79 -0.054569377998504365 1104 80-81 -0.053887559975486 1104 82-83 0.05285109611790517 1104 84-85 0.09685420180712612 1104 86-87 0.10265805277811424 1104 88-89 0.07689959817068726 1104 90-91 0.024928082995000267 1104 92-93 0.04729395064081032 1104 94-95 0.03700038077194634 1104 96-97 -0.018620972501178557 1104 98-99 -0.005193228741806166 1104 100 -0.05586359740223301 1105 1 -0.32829734511967246 1105 2 -0.30826962374875677 1105 3 -0.2539670470075315 1105 4 -0.14842450458776568 1105 5 -0.23813663593583811 1105 6 -0.2315847822969559 1105 7 -0.27374133182520666 1105 8 -0.22099312189914855 1105 9 -0.2980657077238291 1105 10-11 -0.3002279134986381 1105 12-13 -0.3287460158470168 1105 14-15 -0.454906299396292 1105 16-17 -0.41716630167631763 1105 18-19 -0.4013277104092694 1105 20-21 -0.42398263456431096 1105 22-23 -0.3963618340099089 1105 24-25 -0.3874391134555424 1105 26-27 -0.38847457345799086 1105 28-29 -0.4417654478165858 1105 30-31 -0.4342601281033964 1105 32-33 -0.502345093266868 1105 34-35 -0.40296544122037403 1105 36-37 -0.38736577641080316 1105 38-39 -0.3916855020170189 1105 40-41 -0.3388850976349502 1105 42-43 -0.401648108981135 1105 44-45 -0.3602992329842465 1105 46-47 -0.33045020303234196 1105 48-49 -0.30962492324735535 1105 50-51 -0.39073061184598856 1105 52-53 -0.3740731552969123 1105 54-55 -0.37025216063764077 1105 56-57 -0.33408134378853305 1105 58-59 -0.36766014579659867 1105 60-61 -0.37894768098505693 1105 62-63 -0.355284820871681 1105 64-65 -0.337447847621263 1105 66-67 -0.30170330272730794 1105 68-69 -0.28997618735537145 1105 70-71 -0.32218437822211143 1105 72-73 -0.3409825119524008 1105 74-75 -0.36084613470362115 1105 76-77 -0.33497859453567713 1105 78-79 -0.3197135710023531 1105 80-81 -0.3629654077051292 1105 82-83 -0.21567446825540415 1105 84-85 -0.2587063115557555 1105 86-87 -0.3007249230181053 1105 88-89 -0.23687092715053026 1105 90-91 -0.25580815691514047 1105 92-93 -0.25060235291142163 1105 94-95 -0.27201033150678455 1105 96-97 -0.24671269111221505 1105 98-99 -0.24144972330725878 1105 100 -0.276345793078864 1106 1 -0.617633796550848 1106 2 -0.6774633241307377 1106 3 -0.5648499656450987 1106 4 -0.48058693721239365 1106 5 -0.5245693780271878 1106 6 -0.5349458761478942 1106 7 -0.5119652487440263 1106 8 -0.5460882412996 1106 9 -0.620245528257179 1106 10-11 -0.7078866097314602 1106 12-13 -0.7130821055849168 1106 14-15 -0.8244486210452209 1106 16-17 -0.8249980863992548 1106 18-19 -0.806796379803437 1106 20-21 -0.7513669035848878 1106 22-23 -0.8607541737897648 1106 24-25 -0.9611022627506003 1106 26-27 -0.8898375344098142 1106 28-29 -0.8778451018445921 1106 30-31 -0.9023631808087131 1106 32-33 -0.9362804164987395 1106 34-35 -0.9251762794155027 1106 36-37 -0.9132143615439219 1106 38-39 -0.9658475756907876 1106 40-41 -0.9142941686249344 1106 42-43 -0.9537485615300199 1106 44-45 -0.9281442645238087 1106 46-47 -0.9438848444007633 1106 48-49 -0.94402853153575 1106 50-51 -0.9513936810214929 1106 52-53 -0.9417070939654479 1106 54-55 -0.8846664492929008 1106 56-57 -0.8640623136885281 1106 58-59 -0.7897510717937237 1106 60-61 -0.7837019457686054 1106 62-63 -0.814009893396566 1106 64-65 -0.8097192206290771 1106 66-67 -0.7437823257843341 1106 68-69 -0.7654154561650337 1106 70-71 -0.7574638820135284 1106 72-73 -0.7832608028962795 1106 74-75 -0.8184656431072383 1106 76-77 -0.8535274688836054 1106 78-79 -0.7650417034964292 1106 80-81 -0.7453717374299629 1106 82-83 -0.6774065769444206 1106 84-85 -0.6942439182017779 1106 86-87 -0.774052135683835 1106 88-89 -0.7206482234451919 1106 90-91 -0.7579498377117488 1106 92-93 -0.7034051610560148 1106 94-95 -0.7651274098135445 1106 96-97 -0.7422695647974997 1106 98-99 -0.755060665793625 1106 100 -0.835129223154194 1107 1 -0.824376934863448 1107 2 -0.8433965372269157 1107 3 -0.7517256083225874 1107 4 -0.6844140633401068 1107 5 -0.7872048018820337 1107 6 -0.7977620479166632 1107 7 -0.8197373596398236 1107 8 -0.8187327045313282 1107 9 -1.036461882564467 1107 10-11 -1.0304200240387473 1107 12-13 -0.999073590492479 1107 14-15 -1.2309300318046965 1107 16-17 -1.2528807817590106 1107 18-19 -1.3587287951037297 1107 20-21 -1.2911534514319598 1107 22-23 -1.2475121958594357 1107 24-25 -1.2296807474402556 1107 26-27 -1.2350808234547515 1107 28-29 -1.3253125210641556 1107 30-31 -1.3110279611303852 1107 32-33 -1.3289894828384305 1107 34-35 -1.3437955895861506 1107 36-37 -1.390679770148985 1107 38-39 -1.415005645410723 1107 40-41 -1.4361379962048488 1107 42-43 -1.4495173455825494 1107 44-45 -1.433467803796809 1107 46-47 -1.2920424610757877 1107 48-49 -1.2392501026090272 1107 50-51 -1.226844448130997 1107 52-53 -1.3050881579550264 1107 54-55 -1.183344313755704 1107 56-57 -1.191733834582969 1107 58-59 -1.2192090883086095 1107 60-61 -1.24497212773759 1107 62-63 -1.2011948414529066 1107 64-65 -1.1107373429914844 1107 66-67 -1.1706995852525743 1107 68-69 -1.2125249328409744 1107 70-71 -1.2185903248431451 1107 72-73 -1.2116271846125493 1107 74-75 -1.1596071576353353 1107 76-77 -1.2135624510565108 1107 78-79 -1.165349413745659 1107 80-81 -1.168526653837148 1107 82-83 -1.1553859797670825 1107 84-85 -1.1309832262112458 1107 86-87 -1.1514950741893983 1107 88-89 -1.1470415197243007 1107 90-91 -1.1996044875916496 1107 92-93 -1.2186969217584895 1107 94-95 -1.2448639505337304 1107 96-97 -1.2496140544429473 1107 98-99 -1.3096569772499613 1107 100 -1.3480009310839804 1108 1 -1.4442056802716792 1108 2 -1.3929925644604104 1108 3 -1.3337306265106434 1108 4 -1.1962936189541225 1108 5 -1.3901752839951982 1108 6 -1.5051640202335221 1108 7 -1.7391022658181683 1108 8 -1.6150541709432815 1108 9 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0.8509623468073144 2305 78-79 0.8424751505855212 2305 80-81 0.8181365868475581 2305 82-83 0.8880173177576758 2305 84-85 0.8722029276283436 2305 86-87 0.8985159965228853 2305 88-89 0.9320194326080191 2305 90-91 0.9301789690244036 2305 92-93 0.9330516633197341 2305 94-95 0.9751846419638142 2305 96-97 1.0441593293011913 2305 98-99 1.0681702120548913 2305 100 1.1114554702308297 2306 1 0.24175978358389116 2306 2 0.030096513118085255 2306 3 0.11556257896912214 2306 4 0.1411797315626444 2306 5 0.2158002330533364 2306 6 0.2283724024835081 2306 7 0.2889762589580869 2306 8 0.2566863198312248 2306 9 0.3346975033303323 2306 10-11 0.26609485419341183 2306 12-13 0.28108441690145014 2306 14-15 0.35856094739971667 2306 16-17 0.36776093931916876 2306 18-19 0.3647517749676723 2306 20-21 0.33730749291956386 2306 22-23 0.3520864709447906 2306 24-25 0.3580347032775961 2306 26-27 0.3746750848278779 2306 28-29 0.40393259177312757 2306 30-31 0.41231146609003844 2306 32-33 0.3936198290125432 2306 34-35 0.4546831952516399 2306 36-37 0.3590869002288599 2306 38-39 0.36324709066141736 2306 40-41 0.41694491674511625 2306 42-43 0.4257936686437418 2306 44-45 0.45205871498419725 2306 46-47 0.49207193004339445 2306 48-49 0.47760641940769233 2306 50-51 0.4853740663047077 2306 52-53 0.5221010782467062 2306 54-55 0.5505475421451607 2306 56-57 0.5701665200499448 2306 58-59 0.5870767996068409 2306 60-61 0.5717382400222917 2306 62-63 0.6287245408681414 2306 64-65 0.604348039983023 2306 66-67 0.5714739508502298 2306 68-69 0.5191898195572229 2306 70-71 0.5283453287666937 2306 72-73 0.5256538949166334 2306 74-75 0.5219461306353921 2306 76-77 0.5289022509178771 2306 78-79 0.4895921373732861 2306 80-81 0.45280536942797056 2306 82-83 0.5031628288869392 2306 84-85 0.4829232427327881 2306 86-87 0.5015997086234769 2306 88-89 0.5840714301490166 2306 90-91 0.5253815736286818 2306 92-93 0.48370502332280907 2306 94-95 0.5683120127715533 2306 96-97 0.6267096053759218 2306 98-99 0.6634962431222 2306 100 0.6664076029254744 2307 1 -0.30426034155187764 2307 2 -0.18321619224816743 2307 3 -0.14220759510541825 2307 4 -0.11297702105458285 2307 5 -0.09580632405526757 2307 6 -0.08077154969817002 2307 7 -0.015816298507878912 2307 8 -0.06439665821091012 2307 9 -0.07411757883888015 2307 10-11 -0.1326472094867981 2307 12-13 -0.15470625491775536 2307 14-15 -0.4152094503962971 2307 16-17 -0.21602628390050427 2307 18-19 -0.2330458949556231 2307 20-21 -0.3098233972055411 2307 22-23 -0.29164674505817345 2307 24-25 -0.25184066400595384 2307 26-27 -0.27778996328101613 2307 28-29 -0.2657515110376565 2307 30-31 -0.31851045353551655 2307 32-33 -0.3239692295227883 2307 34-35 -0.2851726005220243 2307 36-37 -0.36525765263146326 2307 38-39 -0.3594887312869659 2307 40-41 -0.34581733798948733 2307 42-43 -0.30926920573101313 2307 44-45 -0.30888658040922934 2307 46-47 -0.21661978538592308 2307 48-49 -0.2228430614162562 2307 50-51 -0.26280831850854014 2307 52-53 -0.2533032618720199 2307 54-55 -0.21710119762009583 2307 56-57 -0.21335053765321987 2307 58-59 -0.20859861009552816 2307 60-61 -0.5130232909964363 2307 62-63 -0.39196953331356355 2307 64-65 -0.2770123699376228 2307 66-67 -0.2756657559828035 2307 68-69 -0.28267916177911445 2307 70-71 -0.32384176551714816 2307 72-73 -0.3406337023206163 2307 74-75 -0.257191010163325 2307 76-77 -0.21290263226757133 2307 78-79 -0.239135610182597 2307 80-81 -0.29044561076902653 2307 82-83 -0.257505801084271 2307 84-85 -0.282521440683972 2307 86-87 -0.26673987667140153 2307 88-89 -0.22515721908709097 2307 90-91 -0.2462032129745566 2307 92-93 -0.25927965093860195 2307 94-95 -0.25121125957949886 2307 96-97 -0.22037854014033442 2307 98-99 -0.13479330434538284 2307 100 -0.15077744191724918 2308 1 -0.591782524261518 2308 2 -0.5664781720107932 2308 3 -0.597464414747062 2308 4 -0.5683703197741252 2308 5 -0.6997114347731852 2308 6 -0.7653844888477792 2308 7 -0.7374365574595316 2308 8 -0.737054445142725 2308 9 -0.8192459081717871 2308 10-11 -0.8880207097391661 2308 12-13 -1.0239135332162093 2308 14-15 -1.2701998408003519 2308 16-17 -1.2567351165182856 2308 18-19 -1.2953310072645294 2308 20-21 -1.193104436298512 2308 22-23 -1.244158029671901 2308 24-25 -1.203604988080329 2308 26-27 -1.2918962916576504 2308 28-29 -1.2984918417003897 2308 30-31 -1.2164624534498572 2308 32-33 -1.2050307615472775 2308 34-35 -1.1479305543858445 2308 36-37 -1.1795890396305708 2308 38-39 -1.1326002573579999 2308 40-41 -1.106564702243638 2308 42-43 -1.100139823144218 2308 44-45 -1.2430717789695862 2308 46-47 -1.5249047827609132 2308 48-49 -1.2738470531364783 2308 50-51 -1.3905727550109503 2308 52-53 -1.4325144310759015 2308 54-55 -1.475266392924091 2308 56-57 -1.536787281701642 2308 58-59 -1.5561661278679786 2308 60-61 -1.5876309988896367 2308 62-63 -1.4917502714057527 2308 64-65 -1.6820629151846305 2308 66-67 -1.7939635119242574 2308 68-69 -1.6697527550408964 2308 70-71 -1.6217175087959994 2308 72-73 -1.553361691552528 2308 74-75 -1.5635136611544063 2308 76-77 -1.5438017711015988 2308 78-79 -1.5324261474686125 2308 80-81 -1.5614117101010052 2308 82-83 -2.684560604571608 2308 84-85 -2.001652159758926 2308 86-87 -1.7165269696070204 2308 88-89 -1.6518607295334036 2308 90-91 -1.7410993065054114 2308 92-93 -1.6828857083305913 2308 94-95 -1.742172609072103 2308 96-97 -1.7578359415304554 2308 98-99 -1.630609463023486 2308 100 -1.5319547482741314 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 567829.0 3 177656.0 4 107592.0 5 87329.0 6 84694.0 7 92409.0 8 99905.0 9 107727.0 10 118367.0 11 120797.0 12 127155.0 13 159399.0 14 238689.0 15 359041.0 16 457691.0 17 417944.0 18 325409.0 19 223827.0 20 179721.0 21 174147.0 22 185319.0 23 208711.0 24 244035.0 25 286519.0 26 340248.0 27 403319.0 28 482888.0 29 579038.0 30 701437.0 31 860052.0 32 1072408.0 33 1381101.0 34 1907136.0 35 2973015.0 36 5186869.0 37 8611859.0 38 7825109.0 39 1339458.0 40 4673.0 >>END_MODULE >>Per base sequence content pass #Base G A T C 1 22.58760119626276 27.208965063642953 26.319218862272052 23.88421487782223 2 25.137095267399882 26.599935098472493 25.431630321270287 22.83133931285733 3 24.902016921488592 25.207322415469797 25.143450628213866 24.74721003482775 4 25.544660053110423 24.691477292048585 25.16947366685524 24.594388987985752 5 26.227301443374916 24.746644650793172 24.606576812880316 24.419477092951595 6 26.070870211225667 24.755681186033872 24.091162348198402 25.08228625454206 7 25.959536003414357 25.100603829055817 24.376044511127834 24.56381565640199 8 25.662283630451604 24.835275170201555 24.518580918553727 24.983860280793117 9 25.429159198929792 24.62184369744734 24.674983575895183 25.274013527727686 10-11 25.24964425416029 24.82700007261184 24.678057566722288 25.245298106505583 12-13 24.828577583898433 24.997017558959016 24.90722360744476 25.267181249697796 14-15 24.622505740126023 25.320578891614304 25.385284631001028 24.67163073725865 16-17 24.307117937980635 25.2601661368255 25.74101727957968 24.691698645614185 18-19 23.787233305932638 25.247520085106245 26.60438385877779 24.360862750183326 20-21 23.434192139183978 24.85526065729904 27.668810402244894 24.041736801272087 22-23 23.35224502462852 24.851300577783412 28.202910626046123 23.593543771541945 24-25 23.292172933700193 24.64417929548907 28.689564486915902 23.374083283894826 26-27 23.2608625565644 24.597392409122104 28.92002105852975 23.221723975783746 28-29 23.28709373437928 24.828720547484213 28.82880241749944 23.055383300637065 30-31 23.250719202420612 24.67587105279137 28.83811908978629 23.235290655001734 32-33 23.35208752795028 24.765024917137268 28.70670328035313 23.176184274559315 34-35 23.441691030666618 24.760019602138616 28.66669934720159 23.131590019993173 36-37 23.424884336395678 24.77593688520936 28.60113226740567 23.19804651098929 38-39 23.490376669124956 24.84673297171766 28.44074499660185 23.222145362555533 40-41 23.618087656178044 25.025619174376462 28.10723599962771 23.24905716981778 42-43 23.714341463434742 25.10334131875155 27.686714895169988 23.495602322643723 44-45 23.94040365619162 25.32058549307979 27.194258247783505 23.544752602945085 46-47 24.264910048046488 25.461322072916172 26.617801189811065 23.65596668922628 48-49 24.37358470959446 25.581331818040255 26.17903590585657 23.866047566508716 50-51 24.44675963441837 25.638377650088845 25.890978448876613 24.023884266616168 52-53 24.466005119587383 25.68700625723912 25.780096689578723 24.066891933594768 54-55 24.557866672821646 25.664037198612906 25.676111023755272 24.10198510481018 56-57 24.558242365891633 25.682382519114693 25.644469141291232 24.114905973702445 58-59 24.58378493646197 25.779989466014086 25.55906737078028 24.077158226743663 60-61 24.548394092255275 25.694634683266226 25.537823726725957 24.219147497752544 62-63 24.53282039994693 25.8038243344658 25.490492743945765 24.172862521641502 64-65 24.63207694862353 25.89703660680367 25.41564210458934 24.05524433998346 66-67 24.63866984165411 25.940613986092565 25.33967151033341 24.081044661919915 68-69 24.648324520524724 26.012815476324104 25.251047549393675 24.0878124537575 70-71 24.696725449260843 26.091349364397253 25.17687245346706 24.035052732874846 72-73 24.60772671096666 26.143293611611302 25.07461749221497 24.17436218520707 74-75 24.625171172359362 26.231915492116027 25.06304912968708 24.07986420583753 76-77 24.473636899383948 26.49105030935366 24.867416641621045 24.16789614964135 78-79 24.492359654019037 26.567824776013254 24.886515117527416 24.05330045244029 80-81 24.492976283235297 26.729190721843878 24.74715917931137 24.030673815609454 82-83 24.54115318858747 26.82622614628316 24.694600073396995 23.938020591732375 84-85 24.42473517021397 26.88731669267514 24.58819063744116 24.09975749966973 86-87 24.44064492652862 27.0292785585896 24.542745825895455 23.987330688986326 88-89 24.469423905512024 27.152599248714253 24.365445379003276 24.01253146677045 90-91 24.523792166402067 27.203825067214567 24.25775463031537 24.01462813606799 92-93 24.419294751178064 27.37077864782721 24.125109829561215 24.084816771433513 94-95 24.495824612525933 27.444363178610747 24.059364146270358 24.00044806259296 96-97 24.42591182339563 27.586482708610323 23.86345020210506 24.124155265888987 98-99 24.3844341841412 27.662331620424375 23.737970421846292 24.215263773588134 100 24.393164243462376 27.90210587141383 23.333010649294554 24.371719235829243 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 77459.0 1 45327.0 2 9957.5 3 5983.0 4 5071.0 5 4877.0 6 4938.5 7 5110.5 8 5559.5 9 6189.5 10 7050.5 11 8552.5 12 10315.0 13 15411.0 14 30046.0 15 44846.5 16 67022.0 17 63579.5 18 41295.5 19 41617.0 20 53799.5 21 61653.5 22 64080.5 23 75116.5 24 84300.5 25 92133.0 26 105821.0 27 126449.5 28 153050.0 29 178490.5 30 200854.0 31 230778.0 32 273586.0 33 327228.0 34 386414.0 35 458491.0 36 557359.0 37 677274.5 38 835701.5 39 959143.0 40 1018428.0 41 1080598.0 42 1151675.5 43 1248561.5 44 1375944.0 45 1502374.5 46 1576370.0 47 1602835.5 48 1683900.0 49 1844485.0 50 1808841.0 51 1638454.5 52 1493762.0 53 1337818.0 54 1219854.0 55 1126893.0 56 1054070.0 57 967547.0 58 913545.0 59 879770.5 60 813463.0 61 760318.5 62 702976.5 63 621442.0 64 546701.0 65 472566.0 66 405992.5 67 341783.0 68 276226.5 69 219847.0 70 171564.0 71 136505.5 72 111137.5 73 92025.5 74 76819.5 75 56434.5 76 38140.5 77 30140.5 78 25483.5 79 16274.0 80 7190.0 81 3113.0 82 1593.0 83 1008.0 84 715.5 85 524.5 86 380.5 87 280.0 88 210.0 89 164.0 90 135.0 91 103.0 92 90.0 93 71.5 94 56.0 95 46.5 96 31.5 97 17.0 98 10.5 99 7.0 100 4.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.05069998801149557 2 0.0044126145444412105 3 0.02968790579374487 4 0.008454291263780535 5 1.2106998458186628E-4 6 0.0022024433365424607 7 0.0023853362919746416 8 0.004361095402065949 9 0.010558848229809995 10-11 0.01168840542638762 12-13 0.004247753288840372 14-15 4.211689889177688E-4 16-17 0.0018340814685593356 18-19 8.73249463260695E-4 20-21 0.002123876644420186 22-23 4.623843028179786E-4 24-25 0.0032250983126914158 26-27 0.005274272200667471 28-29 0.007448380008903539 30-31 0.007390420973731369 32-33 0.008143888430969578 34-35 6.90356507828514E-4 36-37 0.002984246322087065 38-39 0.0022024433365424607 40-41 0.002485798619606403 42-43 0.0031516835348066674 44-45 0.001174636446155979 46-47 0.0010535664615741128 48-49 7.959707496978017E-4 50-51 0.0014773114076106447 52-53 5.306471664652011E-4 54-55 0.002601716689950743 56-57 0.001163044639121545 58-59 0.0036269476232184614 60-61 0.0013665452515038308 62-63 3.773777178987959E-4 64-65 1.9190880534785182E-4 66-67 2.575957118763112E-6 68-69 9.65983919536167E-5 70-71 0.0024535991556218642 72-73 5.177673808713855E-4 74-75 5.151914237526224E-6 76-77 0.003500725724399069 78-79 0.002096829094673173 80-81 1.287978559381556E-4 82-83 0.007355645552628066 84-85 4.185930317990057E-4 86-87 2.575957118763112E-4 88-89 1.6743721271960227E-5 90-91 0.002091677180435647 92-93 0.006236392184525494 94-95 1.3652572729444494E-4 96-97 0.0025360297834222836 98-99 0.0020350061238228584 100 7.676352213914073E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 3.8820522E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 26.489382344254853 #Duplication Level Percentage of deduplicated Percentage of total 1 65.72918353949363 17.411254739533494 2 15.903356256837856 8.425401688885513 3 6.755974415582452 5.36884768207102 4 3.3378531927986144 3.536706777321372 5 1.8881678450889456 2.500819998934442 6 1.2281556081621077 1.9519850089708148 7 0.8025594313653079 1.4881512541996382 8 0.5536336463159319 1.1732330668725346 9 0.4228036241908988 1.0079826171956423 >10 2.507719144669569 13.142688307199373 >50 0.5042312553412311 9.565341203357859 >100 0.3254268419283046 14.305346589664945 >500 0.022127681530402236 3.940791126856962 >1k 0.017827478037849245 12.968678019744939 >5k 7.546297685231984E-4 1.163578296493721 >10k+ 2.2540888825027023E-4 2.049193622697657 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 238617 0.6146671598078974 No Hit GATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 53443 0.137666876298057 No Hit ATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTT 51240 0.13199204276542184 No Hit ATATGGATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTT 42036 0.10828293344432617 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 2.575957118763112E-5 5.151914237526224E-6 0.0 0.0 2.575957118763112E-6 2 3.091148542515734E-5 5.151914237526224E-6 0.0 0.0 2.575957118763112E-6 3 3.3487442543920454E-5 5.151914237526224E-6 0.0 0.0 2.575957118763112E-6 4 4.121531390020979E-5 5.151914237526224E-6 2.575957118763112E-6 0.0 2.575957118763112E-6 5 4.894318525649913E-5 1.0303828475052448E-5 2.575957118763112E-6 0.0 2.575957118763112E-6 6 4.894318525649913E-5 1.545574271257867E-5 2.575957118763112E-6 0.0 2.575957118763112E-6 7 5.6671056612788466E-5 1.545574271257867E-5 2.575957118763112E-6 0.0 5.151914237526224E-6 8 5.924701373155158E-5 2.0607656950104896E-5 2.575957118763112E-6 0.0 5.151914237526224E-6 9 7.985467068165646E-5 2.3183614068868008E-5 2.575957118763112E-6 0.0 5.151914237526224E-6 10-11 1.8933284822908873E-4 2.7047549747012677E-5 2.575957118763112E-6 0.0 6.439892796907779E-6 12-13 5.911821587561342E-4 3.2199463984538895E-5 2.575957118763112E-6 0.0 7.727871356289335E-6 14-15 0.0013820009942164097 3.6063399662683567E-5 2.575957118763112E-6 2.575957118763112E-6 7.727871356289335E-6 16-17 0.002807793259451792 3.6063399662683567E-5 2.575957118763112E-6 2.575957118763112E-6 1.0303828475052448E-5 18-19 0.004911062246921873 3.863935678144668E-5 5.151914237526224E-6 2.575957118763112E-6 1.0303828475052448E-5 20-21 0.00794553973282482 3.863935678144668E-5 6.439892796907779E-6 2.575957118763112E-6 1.0303828475052448E-5 22-23 0.011884178167413616 3.863935678144668E-5 7.727871356289335E-6 2.575957118763112E-6 1.0303828475052448E-5 24-25 0.0168853989134922 3.863935678144668E-5 7.727871356289335E-6 2.575957118763112E-6 1.0303828475052448E-5 26-27 0.0224996974538364 4.3791271018972904E-5 7.727871356289335E-6 2.575957118763112E-6 1.0303828475052448E-5 28-29 0.02847591796936682 4.6367228137736016E-5 7.727871356289335E-6 2.575957118763112E-6 1.0303828475052448E-5 30-31 0.03305339376940887 4.6367228137736016E-5 7.727871356289335E-6 2.575957118763112E-6 1.0303828475052448E-5 32-33 0.035968089249289334 4.6367228137736016E-5 7.727871356289335E-6 2.575957118763112E-6 1.1591807034434004E-5 34-35 0.03862776497441225 4.6367228137736016E-5 7.727871356289335E-6 2.575957118763112E-6 1.287978559381556E-5 36-37 0.041179250500547104 5.151914237526224E-5 7.727871356289335E-6 2.575957118763112E-6 1.4167764153197115E-5 38-39 0.04359421029938752 5.6671056612788466E-5 7.727871356289335E-6 2.575957118763112E-6 1.545574271257867E-5 40-41 0.04585718862822092 5.795903517217002E-5 7.727871356289335E-6 2.575957118763112E-6 1.545574271257867E-5 42-43 0.04836617086189619 6.18229708503147E-5 7.727871356289335E-6 3.863935678144668E-6 1.6743721271960227E-5 44-45 0.051271850491860976 6.43989279690778E-5 7.727871356289335E-6 5.151914237526224E-6 1.8031699831341783E-5 46-47 0.05486531067253552 6.43989279690778E-5 7.727871356289335E-6 6.439892796907779E-6 1.8031699831341783E-5 48-49 0.05893918685585938 6.697488508784091E-5 7.727871356289335E-6 1.0303828475052448E-5 1.8031699831341783E-5 50-51 0.06379615400328723 6.955084220660403E-5 7.727871356289335E-6 1.287978559381556E-5 1.931967839072334E-5 52-53 0.06989215652484013 6.955084220660403E-5 7.727871356289335E-6 1.545574271257867E-5 2.3183614068868008E-5 54-55 0.07741652726874719 7.212679932536713E-5 7.727871356289335E-6 1.545574271257867E-5 2.3183614068868008E-5 56-57 0.0867659636312979 7.341477788474869E-5 7.727871356289335E-6 1.6743721271960227E-5 2.3183614068868008E-5 58-59 0.0986166543561676 7.470275644413025E-5 7.727871356289335E-6 1.8031699831341783E-5 2.3183614068868008E-5 60-61 0.1135469018165186 7.470275644413025E-5 7.727871356289335E-6 1.8031699831341783E-5 2.3183614068868008E-5 62-63 0.13217879965653218 7.85666921222749E-5 7.727871356289335E-6 1.8031699831341783E-5 2.3183614068868008E-5 64-65 0.15568827230092372 8.500658491918269E-5 7.727871356289335E-6 1.931967839072334E-5 2.3183614068868008E-5 66-67 0.1847399166863341 9.273445627547203E-5 7.727871356289335E-6 2.0607656950104896E-5 2.4471592628249564E-5 68-69 0.22027782109679 9.273445627547203E-5 7.727871356289335E-6 2.0607656950104896E-5 2.8335528306394233E-5 70-71 0.26324220988063995 9.531041339423514E-5 7.727871356289335E-6 2.0607656950104896E-5 2.8335528306394233E-5 72-73 0.314970004782522 1.0175030619114292E-4 7.727871356289335E-6 2.3183614068868008E-5 2.8335528306394233E-5 74-75 0.377344230456252 1.0432626330990604E-4 7.727871356289335E-6 2.3183614068868008E-5 2.962350686577579E-5 76-77 0.45175847970308075 1.0561424186928759E-4 7.727871356289335E-6 2.3183614068868008E-5 3.091148542515734E-5 78-79 0.5400571378200427 1.1076615610681381E-4 7.727871356289335E-6 2.3183614068868008E-5 3.091148542515734E-5 80-81 0.6430451914067512 1.1334211322557693E-4 7.727871356289335E-6 2.4471592628249564E-5 3.091148542515734E-5 82-83 0.7640056978110701 1.172060489037216E-4 7.727871356289335E-6 2.7047549747012677E-5 3.091148542515734E-5 84-85 0.9044443039689163 1.2106998458186626E-4 7.727871356289335E-6 2.8335528306394233E-5 3.091148542515734E-5 86-87 1.0671147080402474 1.249339202600109E-4 7.727871356289335E-6 2.8335528306394233E-5 3.3487442543920454E-5 88 1.2032192663457746 1.262218988193925E-4 7.727871356289335E-6 2.8335528306394233E-5 3.3487442543920454E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ATCTCGA 32265 0.0 43.38875 1 TATCTCG 14305 0.0 37.20742 2 TCTCGAG 38655 0.0 36.10183 2 ATATCTC 17340 0.0 31.062204 1 CTCGAGG 47010 0.0 29.565914 3 TATGGAT 21280 0.0 29.20995 2 GATCCGG 56460 0.0 25.903337 1 ATATGGA 25410 0.0 24.714535 1 TCGAGGG 57925 0.0 23.906792 4 ATCCGGC 66105 0.0 22.198849 2 AACGGTA 3425 0.0 21.748682 64-65 ATTCGTG 5760 0.0 21.223738 2 ACGGTAC 3570 0.0 20.99742 66-67 ACGTAAC 2990 0.0 19.17628 14-15 TCCGGCG 79560 0.0 18.53354 3 TAACGGT 4110 0.0 18.181072 64-65 CGAGGGC 76710 0.0 18.131002 5 CACGTTA 4210 0.0 18.08531 74-75 GCTTACG 4590 0.0 17.815992 68-69 TTACGTC 4725 0.0 17.00894 70-71 >>END_MODULE