FastQCFastQC Report
Wed 25 May 2016
SRR491074_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR491074_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33945612
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA412950.12165048018577482No Hit
ATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTT362620.1068238215884869No Hit
GATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT342520.10090258499390141No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCTCGA243800.047.2348021
TCTCGAG298350.038.4209982
CTCGAGG360800.031.6793943
TCGAGGG417950.027.295274
TATCTCG124950.025.2491762
GATCCGG326750.023.3517631
ATCCGGC386300.019.7661612
CGAGGGC586700.019.5790625
AGGGCGC609550.018.6831827
ATATCTC179300.018.2080551
GGGCGCG644950.017.7524388
GAGGGCG694650.016.529676
GGATCCG231700.016.3151452
TCCGGCG469400.016.2767873
GCGCCGT514800.014.5896559
CGGCGCG528100.014.4714195
TATGGAT229450.014.2620562
CGTCGAC494950.013.02804112-13
GGCGCGC1138650.012.9364619
CCGGCGC596000.012.9341994
Produced by FastQC (version 0.11.5)