##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR491074_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 33945612 Sequences flagged as poor quality 0 Sequence length 100 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.612979550935773 33.0 31.0 34.0 30.0 34.0 2 31.883185667708688 34.0 31.0 34.0 30.0 34.0 3 31.940082093673844 34.0 31.0 34.0 30.0 34.0 4 35.36099086974776 37.0 35.0 37.0 33.0 37.0 5 35.14408118492605 37.0 35.0 37.0 32.0 37.0 6 35.1134957295806 37.0 35.0 37.0 32.0 37.0 7 35.03450554964218 37.0 35.0 37.0 32.0 37.0 8 35.027722110298086 37.0 35.0 37.0 32.0 37.0 9 36.66463255987254 39.0 37.0 39.0 32.0 39.0 10-11 36.658836214235876 39.0 37.0 39.0 32.0 39.0 12-13 36.631244326954544 39.0 37.0 39.0 32.0 39.0 14-15 37.93658660506695 40.0 38.0 41.0 33.0 41.0 16-17 37.89415659968069 40.0 38.0 41.0 33.0 41.0 18-19 37.8732344993515 40.0 38.0 41.0 33.0 41.0 20-21 37.838011596314715 40.0 38.0 41.0 32.5 41.0 22-23 37.75836451262096 40.0 38.0 41.0 32.0 41.0 24-25 37.698611988495 40.0 38.0 41.0 32.0 41.0 26-27 37.52370617150753 40.0 38.0 41.0 32.0 41.0 28-29 37.36491342386168 40.0 37.5 41.0 32.0 41.0 30-31 37.215901321796764 40.0 37.0 41.0 31.0 41.0 32-33 37.07678519686138 40.0 37.0 41.0 31.0 41.0 34-35 36.94914310279632 40.0 37.0 41.0 31.0 41.0 36-37 36.804631287837736 40.0 36.5 41.0 30.0 41.0 38-39 36.64823279662774 40.0 36.0 41.0 30.0 41.0 40-41 36.42170809882585 40.0 36.0 41.0 30.0 41.0 42-43 36.16105155211224 40.0 36.0 41.0 29.5 41.0 44-45 35.86802862767653 40.0 35.0 41.0 28.0 41.0 46-47 35.53908094218481 39.5 35.0 41.0 26.5 41.0 48-49 35.25537396409291 39.0 35.0 41.0 26.0 41.0 50-51 35.026456291316826 39.0 35.0 41.0 24.5 41.0 52-53 34.853313382595665 39.0 35.0 41.0 24.0 41.0 54-55 34.73117422363751 39.0 35.0 41.0 24.0 41.0 56-57 34.495278520829146 38.5 34.0 41.0 23.0 41.0 58-59 34.24132011524789 38.0 34.0 40.0 22.5 41.0 60-61 33.934086871080716 37.5 34.0 40.0 22.0 41.0 62-63 33.597166652938824 37.0 33.5 40.0 20.5 41.0 64-65 33.26113092319561 36.5 33.0 40.0 20.0 41.0 66-67 32.99361166326888 36.0 33.0 39.0 19.5 41.0 68-69 32.859783040588574 36.0 33.0 39.0 19.5 41.0 70-71 32.569944077013545 35.0 33.0 39.0 18.5 41.0 72-73 32.21645732885888 35.0 33.0 37.5 17.5 40.0 74-75 31.88324320091799 35.0 33.0 37.0 14.0 39.5 76-77 31.18108308078228 35.0 31.5 36.0 10.5 39.0 78-79 31.243037332778094 35.0 32.0 36.0 7.5 39.0 80-81 31.02497377275154 35.0 32.0 36.0 4.5 37.5 82-83 30.669577337418456 35.0 32.0 35.5 2.0 37.0 84-85 30.45420162111085 35.0 32.0 35.0 2.0 37.0 86-87 30.20707454029699 35.0 32.0 35.0 2.0 36.0 88-89 29.973891073167277 35.0 32.0 35.0 2.0 36.0 90-91 29.7725247817008 35.0 31.0 35.0 2.0 36.0 92-93 29.55912266363028 35.0 31.0 35.0 2.0 35.5 94-95 29.330134201145057 35.0 31.0 35.0 2.0 35.0 96-97 29.090342913835226 35.0 31.0 35.0 2.0 35.0 98-99 28.79307098661235 34.5 31.0 35.0 2.0 35.0 100 28.175153684075575 34.0 29.0 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.3880322146970947 1101 2 0.48186360156703856 1101 3 0.45946213705964567 1101 4 0.3632075895570921 1101 5 0.4897343076411076 1101 6 0.42579551719681064 1101 7 0.5494133963410945 1101 8 0.4665975337534718 1101 9 0.6304815429236044 1101 10-11 0.6261830992639759 1101 12-13 0.639391382282902 1101 14-15 0.6200179855929235 1101 16-17 0.6526736607597243 1101 18-19 0.7404975341049678 1101 20-21 0.7445706102523175 1101 22-23 0.7394097918903171 1101 24-25 0.7452194349276269 1101 26-27 0.7509722413258757 1101 28-29 0.7447393717504767 1101 30-31 0.7364256360549604 1101 32-33 0.6507504848670465 1101 34-35 0.7278983854948464 1101 36-37 0.7630172936233208 1101 38-39 0.6352188688179226 1101 40-41 0.771848428345713 1101 42-43 0.731043151434271 1101 44-45 0.7308539028478833 1101 46-47 0.7736233182635317 1101 48-49 0.7933013748412989 1101 50-51 0.7641442812689547 1101 52-53 0.7574984956103563 1101 54-55 0.7404999171339597 1101 56-57 0.7729547853794259 1101 58-59 0.697554671493414 1101 60-61 0.7224074385546331 1101 62-63 0.7452748744509492 1101 64-65 0.7238607507773125 1101 66-67 0.6794632432113232 1101 68-69 0.7651935737139155 1101 70-71 0.7565226091132402 1101 72-73 0.7582935222058325 1101 74-75 0.7482664788394899 1101 76-77 0.7782498079495603 1101 78-79 0.7530302494223413 1101 80-81 0.7960008029137242 1101 82-83 0.8886396744468392 1101 84-85 0.8667032689184317 1101 86-87 0.8497937132341278 1101 88-89 0.8467296712407446 1101 90-91 0.8548589246625866 1101 92-93 0.9319499748024107 1101 94-95 0.8833514396631514 1101 96-97 0.9025237622670375 1101 98-99 0.91195025799626 1101 100 0.8796300018105612 1102 1 0.2135230960725032 1102 2 0.20766876057587424 1102 3 0.2718028470567546 1102 4 0.2763924584531523 1102 5 0.31863019022986805 1102 6 0.33002922488147846 1102 7 0.3647658204346058 1102 8 0.3769172874279789 1102 9 0.46383543954829065 1102 10-11 0.3528146319813956 1102 12-13 0.3729625902094611 1102 14-15 0.4281408249432985 1102 16-17 0.424586379644488 1102 18-19 0.4325447394738404 1102 20-21 0.4748302861772373 1102 22-23 0.37943398509327153 1102 24-25 0.3797340231156525 1102 26-27 0.44739608204765346 1102 28-29 0.458532248006442 1102 30-31 0.40549369207980845 1102 32-33 0.42499998019759744 1102 34-35 0.45467016413452654 1102 36-37 0.4327499684066467 1102 38-39 0.42277037319887967 1102 40-41 0.45326077731633063 1102 42-43 0.46422185927804804 1102 44-45 0.4231036205236691 1102 46-47 0.5112762455973652 1102 48-49 0.4698578178615236 1102 50-51 0.45255158164223985 1102 52-53 0.4008873124219221 1102 54-55 0.3828001450742704 1102 56-57 0.4966871871299219 1102 58-59 0.40352079355998427 1102 60-61 0.50605115159712 1102 62-63 0.4638320376236109 1102 64-65 0.48204900302953035 1102 66-67 0.48362579313069887 1102 68-69 0.5949326378865862 1102 70-71 0.5365590693607629 1102 72-73 0.5011651458435153 1102 74-75 0.4768093296504503 1102 76-77 0.5031057349523103 1102 78-79 0.5234745148138238 1102 80-81 0.5463106093510959 1102 82-83 0.5811224627306864 1102 84-85 0.5279281636829758 1102 86-87 0.5026975688350603 1102 88-89 0.4891395406489458 1102 90-91 0.46645645418507087 1102 92-93 0.5330017252934773 1102 94-95 0.5586446402468184 1102 96-97 0.5671733681062463 1102 98-99 0.6205685612526644 1102 100 0.6189582649274143 1103 1 0.04336909488053209 1103 2 0.07711513635133471 1103 3 0.09153586209203013 1103 4 0.13055858503759765 1103 5 0.16218594474359804 1103 6 0.1717642000454589 1103 7 0.22035458600660718 1103 8 0.18739976443735884 1103 9 0.1666167356815791 1103 10-11 0.13928697436846704 1103 12-13 0.18487714270948885 1103 14-15 0.10058561616977357 1103 16-17 0.1727599860666018 1103 18-19 0.26786604969675665 1103 20-21 0.1994082942415929 1103 22-23 0.13723137649759565 1103 24-25 0.1538145357267382 1103 26-27 0.15792323283805842 1103 28-29 0.1670573984329664 1103 30-31 0.2594493451204727 1103 32-33 0.2339075913291424 1103 34-35 0.11609853782788804 1103 36-37 0.21060486043830906 1103 38-39 0.18523290249658686 1103 40-41 0.21094307667546985 1103 42-43 0.19998035232751477 1103 44-45 0.3051952751322844 1103 46-47 0.18990606154202538 1103 48-49 0.11215581385302187 1103 50-51 0.13345533857069114 1103 52-53 0.10465548822664772 1103 54-55 0.14388153324009778 1103 56-57 0.22715389666701924 1103 58-59 0.20749510938406246 1103 60-61 0.266169516925558 1103 62-63 0.27481987772564764 1103 64-65 0.29618792803302085 1103 66-67 0.17135523355046445 1103 68-69 0.17395548250297566 1103 70-71 0.2797453410920525 1103 72-73 0.22478766384111992 1103 74-75 0.2550207494607619 1103 76-77 0.22340307624694944 1103 78-79 0.25369652825451183 1103 80-81 0.29927389119475123 1103 82-83 0.3541159714601001 1103 84-85 0.3116304764569371 1103 86-87 0.3147311177473284 1103 88-89 0.35316381087330484 1103 90-91 0.3581329163150784 1103 92-93 0.2358527995736246 1103 94-95 0.3194751601969372 1103 96-97 0.18618458695404172 1103 98-99 0.3596984087133137 1103 100 0.42469137956418734 1104 1 -0.15652693521441563 1104 2 -0.14961125653760377 1104 3 -0.07422493198527391 1104 4 -0.07362637497588764 1104 5 -0.08944898403407109 1104 6 -0.015758230299880438 1104 7 -0.02165958825495551 1104 8 -0.014075138153700095 1104 9 -0.0353033986171809 1104 10-11 -0.05285450778063705 1104 12-13 -0.10066294817411148 1104 14-15 -0.16468600760921248 1104 16-17 -0.1896625738408133 1104 18-19 -0.07548877466658865 1104 20-21 -0.17352586649030144 1104 22-23 -0.12539651078707692 1104 24-25 -0.052275919279445304 1104 26-27 -0.10405924121389631 1104 28-29 -0.06739492914974932 1104 30-31 -0.072784046063191 1104 32-33 -0.13261933931497083 1104 34-35 -0.12123968754739423 1104 36-37 -0.1251612975516494 1104 38-39 -0.06904317777047453 1104 40-41 -0.06945571490451385 1104 42-43 -0.07912899098683823 1104 44-45 -0.06282386660735284 1104 46-47 -0.10666319605945773 1104 48-49 -0.03991394664840442 1104 50-51 -0.07956976343619004 1104 52-53 -0.0647210272287495 1104 54-55 -0.11509228977959651 1104 56-57 -0.052534339508625294 1104 58-59 -0.007164689790833734 1104 60-61 0.05891946163266937 1104 62-63 0.07117572895612057 1104 64-65 -0.04763237481629545 1104 66-67 0.028837821612974324 1104 68-69 0.029793500058445943 1104 70-71 -0.06306170535673772 1104 72-73 -0.10264911656648223 1104 74-75 -0.05735725596888841 1104 76-77 -0.060608383712665415 1104 78-79 -0.04392574574414709 1104 80-81 -0.06138277162416017 1104 82-83 0.0653330932432361 1104 84-85 0.09706144726256838 1104 86-87 0.0794347960856463 1104 88-89 0.08970968664819523 1104 90-91 0.02606784406583529 1104 92-93 0.0529005546758512 1104 94-95 0.021834676224365523 1104 96-97 -0.0334699573336259 1104 98-99 -0.0020718914819291 1104 100 -0.057373657845136705 1105 1 -0.33174004668953216 1105 2 -0.3133942603937463 1105 3 -0.26666994663021626 1105 4 -0.16915399574580192 1105 5 -0.26720714304856585 1105 6 -0.23614953965281416 1105 7 -0.2858303072907944 1105 8 -0.23730118264396083 1105 9 -0.2995314838512826 1105 10-11 -0.32752522247619 1105 12-13 -0.31927757624409736 1105 14-15 -0.4732167781205803 1105 16-17 -0.42242493711331264 1105 18-19 -0.4229147373308777 1105 20-21 -0.4647423174795122 1105 22-23 -0.42428795468617864 1105 24-25 -0.4039571945569307 1105 26-27 -0.42199093070879456 1105 28-29 -0.4532675755419433 1105 30-31 -0.4573054638246248 1105 32-33 -0.5238206286232767 1105 34-35 -0.42728727786559517 1105 36-37 -0.4057815992291509 1105 38-39 -0.4072102995642126 1105 40-41 -0.38123481461749265 1105 42-43 -0.39938860326535774 1105 44-45 -0.35864776926915454 1105 46-47 -0.34292947544224717 1105 48-49 -0.3249399522597898 1105 50-51 -0.3870844532295905 1105 52-53 -0.36123858476746307 1105 54-55 -0.3738859597351052 1105 56-57 -0.33241479695146836 1105 58-59 -0.3868541014298401 1105 60-61 -0.3784019722955634 1105 62-63 -0.3675655651503007 1105 64-65 -0.3243371826179029 1105 66-67 -0.26069612435266265 1105 68-69 -0.242924317743217 1105 70-71 -0.28944841583138015 1105 72-73 -0.31396556173702095 1105 74-75 -0.3350548848520738 1105 76-77 -0.287356486282178 1105 78-79 -0.2716264386420626 1105 80-81 -0.30948793242868433 1105 82-83 -0.16947346539254227 1105 84-85 -0.20883672218421978 1105 86-87 -0.22284389450882358 1105 88-89 -0.1842714299923216 1105 90-91 -0.19424571745217634 1105 92-93 -0.2302243038198739 1105 94-95 -0.23597168996924722 1105 96-97 -0.22519635346377243 1105 98-99 -0.22501752993383306 1105 100 -0.280576182731032 1106 1 -0.5395937334621834 1106 2 -0.5844814705833876 1106 3 -0.4897245166200932 1106 4 -0.38544703680224046 1106 5 -0.4264763954966 1106 6 -0.4532560001315389 1106 7 -0.42448681901318963 1106 8 -0.43985199008909603 1106 9 -0.5152013217116291 1106 10-11 -0.5940097982868409 1106 12-13 -0.5929076702766523 1106 14-15 -0.7067255124542271 1106 16-17 -0.7030861103625554 1106 18-19 -0.688298960406577 1106 20-21 -0.6472118048213602 1106 22-23 -0.7505450910993545 1106 24-25 -0.8481428080466173 1106 26-27 -0.7891852081825945 1106 28-29 -0.7604854771942797 1106 30-31 -0.7841492404332371 1106 32-33 -0.8121191007684416 1106 34-35 -0.8172656704490819 1106 36-37 -0.8064310833796569 1106 38-39 -0.8750774410754403 1106 40-41 -0.8203162033776081 1106 42-43 -0.8545492407169988 1106 44-45 -0.8422657309215253 1106 46-47 -0.8767622021984423 1106 48-49 -0.8566925477863521 1106 50-51 -0.8806097552047163 1106 52-53 -0.8906891364449692 1106 54-55 -0.8287831674833797 1106 56-57 -0.8202885849427588 1106 58-59 -0.7483080250305605 1106 60-61 -0.7446967417149253 1106 62-63 -0.7708196702605434 1106 64-65 -0.7491704569114859 1106 66-67 -0.6911061315963067 1106 68-69 -0.7177865807925485 1106 70-71 -0.7107737473086431 1106 72-73 -0.7181622075664791 1106 74-75 -0.7323459062394946 1106 76-77 -0.8023260031145796 1106 78-79 -0.7145142904879584 1106 80-81 -0.672776176160049 1106 82-83 -0.6021080196034418 1106 84-85 -0.6176317826151205 1106 86-87 -0.6833827460321622 1106 88-89 -0.6323773290547621 1106 90-91 -0.690164163156048 1106 92-93 -0.6463256851435837 1106 94-95 -0.6905646534679306 1106 96-97 -0.6633582034703771 1106 98-99 -0.6871614110480913 1106 100 -0.7790358475966066 1107 1 -0.8139888711334713 1107 2 -0.8255957402201517 1107 3 -0.7561929371288691 1107 4 -0.6850600623841032 1107 5 -0.7607439523169717 1107 6 -0.7596924836165186 1107 7 -0.7889027534364246 1107 8 -0.7924468428626952 1107 9 -0.9650502849578757 1107 10-11 -0.9910301703551099 1107 12-13 -0.9774076800853564 1107 14-15 -1.2030712626412026 1107 16-17 -1.2531026987022997 1107 18-19 -1.3247148144441283 1107 20-21 -1.25969262903169 1107 22-23 -1.216464644623919 1107 24-25 -1.209325396409227 1107 26-27 -1.2059273909713184 1107 28-29 -1.2792125850349478 1107 30-31 -1.2812017255764445 1107 32-33 -1.3046832455917752 1107 34-35 -1.3177643086365052 1107 36-37 -1.37465831022444 1107 38-39 -1.3904573405791112 1107 40-41 -1.40490871758562 1107 42-43 -1.4657077889614456 1107 44-45 -1.4609222783320135 1107 46-47 -1.3286011198879066 1107 48-49 -1.2940766447583485 1107 50-51 -1.275918545486789 1107 52-53 -1.3122864927251143 1107 54-55 -1.2513964156751385 1107 56-57 -1.251480496620438 1107 58-59 -1.2577216272122982 1107 60-61 -1.305941444314513 1107 62-63 -1.2597638045404338 1107 64-65 -1.205862206180953 1107 66-67 -1.2462228669198439 1107 68-69 -1.3050551179768703 1107 70-71 -1.317027355354309 1107 72-73 -1.283973425104243 1107 74-75 -1.245958227712915 1107 76-77 -1.2928435538570717 1107 78-79 -1.2517067282796184 1107 80-81 -1.270800991538465 1107 82-83 -1.1886891405202853 1107 84-85 -1.186204635687968 1107 86-87 -1.2121853401061848 1107 88-89 -1.2129484494393772 1107 90-91 -1.2392195472458916 1107 92-93 -1.260804729399478 1107 94-95 -1.294034759077114 1107 96-97 -1.302277242999235 1107 98-99 -1.3339664457921465 1107 100 -1.3582800749253678 1108 1 -1.38561697823409 1108 2 -1.3364798479224227 1108 3 -1.2799003901859614 1108 4 -1.1325455910518087 1108 5 -1.3457131344812723 1108 6 -1.4235606552945512 1108 7 -1.6909927278990722 1108 8 -1.558886345480559 1108 9 -1.98148123843594 1108 10-11 -1.8095339151905634 1108 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0.7718761301120161 2305 88-89 0.7950754645794831 2305 90-91 0.7995325436410994 2305 92-93 0.7886325425877629 2305 94-95 0.8497957074574956 2305 96-97 0.9217704164872664 2305 98-99 0.9409165325663906 2305 100 0.9930866999311547 2306 1 0.21581503467772833 2306 2 -0.007788934801418179 2306 3 0.0849214549076791 2306 4 0.12319640960967604 2306 5 0.18869785707108377 2306 6 0.19965685018813417 2306 7 0.26932243648911935 2306 8 0.22645220694959534 2306 9 0.3252804086625716 2306 10-11 0.24529387427457294 2306 12-13 0.23297054829135533 2306 14-15 0.30241020967985577 2306 16-17 0.34295958150220685 2306 18-19 0.3322272477713142 2306 20-21 0.303276485980966 2306 22-23 0.3205095026944207 2306 24-25 0.33556458061260486 2306 26-27 0.35138519555033554 2306 28-29 0.36855587233934983 2306 30-31 0.38182503451497496 2306 32-33 0.3605616332203141 2306 34-35 0.41166647509708554 2306 36-37 0.31109675445831186 2306 38-39 0.31956329816043194 2306 40-41 0.3462534996749653 2306 42-43 0.36299398031132313 2306 44-45 0.39694049582616486 2306 46-47 0.40473980524502196 2306 48-49 0.37985404712713233 2306 50-51 0.3831945206493401 2306 52-53 0.4435464717864974 2306 54-55 0.46077315642518357 2306 56-57 0.4711247055651171 2306 58-59 0.4846473815358152 2306 60-61 0.45868219811850963 2306 62-63 0.499066328078726 2306 64-65 0.5103793921040207 2306 66-67 0.49138884056741716 2306 68-69 0.4429915588834348 2306 70-71 0.46462336940596316 2306 72-73 0.46081069956628085 2306 74-75 0.4551792586705403 2306 76-77 0.4572068101971283 2306 78-79 0.4287553052986226 2306 80-81 0.4083236106007959 2306 82-83 0.4473022067643626 2306 84-85 0.44212725763212646 2306 86-87 0.4541844045358836 2306 88-89 0.5087797307534743 2306 90-91 0.47550910711094474 2306 92-93 0.41869222446183585 2306 94-95 0.5191728858303293 2306 96-97 0.5887242881061958 2306 98-99 0.5734069951345511 2306 100 0.5732561617613889 2307 1 -0.3030831241251093 2307 2 -0.19488346150701474 2307 3 -0.14896472158809004 2307 4 -0.10378218781321635 2307 5 -0.11391223578515763 2307 6 -0.09309507392821814 2307 7 -0.027978617706182263 2307 8 -0.09659143027932515 2307 9 -0.08412266012617664 2307 10-11 -0.14270853562397434 2307 12-13 -0.16219173106225782 2307 14-15 -0.42862342672895437 2307 16-17 -0.22902938043763754 2307 18-19 -0.23522133697414915 2307 20-21 -0.34309579933856327 2307 22-23 -0.306464003756993 2307 24-25 -0.28492075179114096 2307 26-27 -0.3061798882028981 2307 28-29 -0.29012183463614605 2307 30-31 -0.34321764344536376 2307 32-33 -0.3211857301540135 2307 34-35 -0.29005093225464407 2307 36-37 -0.38779250665582055 2307 38-39 -0.38302990469725984 2307 40-41 -0.36288524602547767 2307 42-43 -0.29670439251297154 2307 44-45 -0.31526250843610626 2307 46-47 -0.2472573970386307 2307 48-49 -0.25245868752998746 2307 50-51 -0.27260803773228304 2307 52-53 -0.2578195924068396 2307 54-55 -0.22608423248244236 2307 56-57 -0.22058316636355357 2307 58-59 -0.20020872437263648 2307 60-61 -0.5329140091897173 2307 62-63 -0.3877562612412504 2307 64-65 -0.2765333308103308 2307 66-67 -0.3055360473848836 2307 68-69 -0.3110965604743967 2307 70-71 -0.34958796258462144 2307 72-73 -0.34540816592624424 2307 74-75 -0.27944490960284796 2307 76-77 -0.2109221764025655 2307 78-79 -0.24402586705717155 2307 80-81 -0.3067069301685308 2307 82-83 -0.2446161040130086 2307 84-85 -0.31598852465007 2307 86-87 -0.2631331920809714 2307 88-89 -0.23316485130676057 2307 90-91 -0.24588995932595736 2307 92-93 -0.26242869754360143 2307 94-95 -0.2804259323451319 2307 96-97 -0.24492248181042342 2307 98-99 -0.17117489519404216 2307 100 -0.16177197279617062 2308 1 -0.5887128890016022 2308 2 -0.5598216121567212 2308 3 -0.5919764392676221 2308 4 -0.5583969711089054 2308 5 -0.6836765810692711 2308 6 -0.7571565014388426 2308 7 -0.730589856007505 2308 8 -0.731700121853649 2308 9 -0.8603536989609921 2308 10-11 -0.9096866392029597 2308 12-13 -1.04719305942956 2308 14-15 -1.284240074363126 2308 16-17 -1.2712820049879028 2308 18-19 -1.3197806670681373 2308 20-21 -1.2195292676873422 2308 22-23 -1.2830674551788732 2308 24-25 -1.2228542149892476 2308 26-27 -1.3350652726491816 2308 28-29 -1.3463701676243076 2308 30-31 -1.2610578393987026 2308 32-33 -1.2750368720564538 2308 34-35 -1.2165617235561044 2308 36-37 -1.2204403492193734 2308 38-39 -1.170427821506685 2308 40-41 -1.1820873018162743 2308 42-43 -1.163257064406686 2308 44-45 -1.32873147602227 2308 46-47 -1.6122096104475645 2308 48-49 -1.3559825897039204 2308 50-51 -1.4888007773118659 2308 52-53 -1.530053747490662 2308 54-55 -1.5821930411998792 2308 56-57 -1.64357899707678 2308 58-59 -1.6885160136994841 2308 60-61 -1.716464789753033 2308 62-63 -1.6156804262180202 2308 64-65 -1.830717599950514 2308 66-67 -1.9352216013989718 2308 68-69 -1.8376567899345417 2308 70-71 -1.7982222994733625 2308 72-73 -1.7224515739996349 2308 74-75 -1.7428832768272784 2308 76-77 -1.7195213930006936 2308 78-79 -1.693198757564442 2308 80-81 -1.7102063505095941 2308 82-83 -2.88783501815254 2308 84-85 -2.171076527627495 2308 86-87 -1.8729558038310152 2308 88-89 -1.819980617053229 2308 90-91 -1.907206711536503 2308 92-93 -1.846741253172457 2308 94-95 -1.904464415154429 2308 96-97 -1.9369463851859763 2308 98-99 -1.7935239079877903 2308 100 -1.688975804820526 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 423093.0 3 132514.0 4 80171.0 5 66259.0 6 63671.0 7 67112.0 8 72958.0 9 79962.0 10 88130.0 11 91154.0 12 94747.0 13 113288.0 14 155554.0 15 228834.0 16 292552.0 17 282363.0 18 224507.0 19 158906.0 20 133095.0 21 130870.0 22 140642.0 23 160701.0 24 190574.0 25 226892.0 26 272135.0 27 324893.0 28 388524.0 29 465457.0 30 562221.0 31 689116.0 32 864735.0 33 1124063.0 34 1561108.0 35 2360439.0 36 4094774.0 37 7238471.0 38 8067100.0 39 2227965.0 40 6062.0 >>END_MODULE >>Per base sequence content pass #Base G A T C 1 20.87881558646785 28.01440942974473 28.244257777582078 22.862517206205343 2 23.62665278506915 27.369390134756372 27.374819630558605 21.62913744961587 3 23.343671440383293 26.244464775102188 26.981425088740846 23.43043869577367 4 24.087644034574307 25.857048362290403 26.98472775979136 23.070579843343932 5 24.62641031177033 26.126292608729194 26.486162550035573 22.761134529464897 6 24.632291206981577 26.04945774589384 25.83383757534908 23.484413471775508 7 24.58653243968743 26.41516037263141 26.036044414648785 22.96226277303238 8 24.110586562551443 26.18544858328878 26.309908900750965 23.394055953408806 9 23.842477785221796 25.923990151584093 26.459278948488073 23.774253114706035 10-11 23.80583836268183 26.190004014738825 26.343621724546495 23.660535898032855 12-13 23.52449939244192 26.267248429020405 26.51019064629526 23.69806153224241 14-15 23.304909290340728 26.4897928618525 26.865999669235073 23.3392981785717 16-17 23.126231207650655 26.412150066003186 27.126939110622878 23.33467961572328 18-19 22.750621478551498 26.38291138760399 27.764720639589513 23.101746494255 20-21 22.435690712332512 26.134669503813612 28.4535587166544 22.976081067199473 22-23 22.457700042026136 26.08923433893028 28.81694597776639 22.636119641277197 24-25 22.36857742011166 25.966575228566775 29.223050025270286 22.44179732605128 26-27 22.411343217855997 26.00893344307691 29.253128547786254 22.326594791280836 28-29 22.43869156528596 26.092092662269334 29.202867537781025 22.266348234663678 30-31 22.437366737425798 26.0299368989117 29.166091213525796 22.366605150136706 32-33 22.474942375834747 26.081946077000033 29.055214689225007 22.38789685794021 34-35 22.521231078504616 26.160969105790688 29.04469868188792 22.273101133816773 36-37 22.518427240482183 26.13011069689357 28.97620412426809 22.375257938356157 38-39 22.5489830439021 26.191058638956022 28.877064202778293 22.382894114363584 40-41 22.611135832266545 26.30500966481796 28.719797167706794 22.364057335208702 42-43 22.699231488562386 26.380275806965585 28.37186525380356 22.54862745066847 44-45 22.760747857045093 26.551442082698763 28.03647461345151 22.651335446804634 46-47 23.008314523726618 26.70302061367925 27.62301659546413 22.66564826713 48-49 23.08951031127366 26.76263531812286 27.264737743052375 22.8831166275511 50-51 23.0732788822539 26.85112926016163 27.0886234990811 22.986968358503372 52-53 23.149620794395876 26.952064052895974 26.932148346077216 22.966166806630934 54-55 23.167213531431916 26.917195745475496 26.879115386771566 23.036475336321022 56-57 23.15566802349945 26.97700356233047 26.843707445543185 23.023620968626894 58-59 23.191239664936838 27.05502927779974 26.804049774277527 22.949681282985892 60-61 23.156092458078916 27.028661969113276 26.7567497502602 23.05849582254761 62-63 23.16718131623016 27.108164461007096 26.739353672460048 22.985300550302696 64-65 23.19294150672216 27.209235046254815 26.673586856311854 22.92423659071117 66-67 23.185053290099862 27.230518843805758 26.610677297869227 22.973750568225153 68-69 23.153119447916502 27.339926924233755 26.549024483003535 22.957929144846208 70-71 23.22637031075362 27.443120121183483 26.483229450273114 22.84728011778978 72-73 23.195575617435903 27.491347718782688 26.375247814310736 22.93782884947067 74-75 23.17313716933833 27.610675500876976 26.350139934143474 22.86604739564122 76-77 23.161685198042022 27.75749024680864 26.226278458389345 22.85454609675999 78-79 23.14362184175423 27.808862512010833 26.168421647394975 22.879093998839963 80-81 23.140153109726477 27.89938840361885 26.095061243596003 22.865397243058673 82-83 23.213650169222174 28.01876708546195 25.993442105035854 22.77414064028003 84-85 23.103101981068807 28.079101054880066 25.933162402805788 22.884634561245335 86-87 23.116999097010634 28.199118397806256 25.866103490785587 22.81777901439753 88-89 23.20343077062735 28.303097636234813 25.69965196824644 22.793819624891402 90-91 23.159979382642017 28.331505410774994 25.620678998538928 22.887836208044064 92-93 23.165711285367667 28.44158694288477 25.536780104328372 22.855921667419192 94-95 23.22802513433258 28.53052678403092 25.446308210784313 22.79513987085219 96-97 23.180964589940896 28.632815310125466 25.263537018321756 22.922683081611883 98-99 23.188144655808824 28.721218958882183 25.174285699766514 22.916350685542483 100 23.218334974500486 28.902011155930207 24.882303481059587 22.997350388509723 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 9105.0 1 5812.5 2 2217.0 3 1947.5 4 2097.0 5 2435.5 6 2837.5 7 3252.5 8 3791.0 9 4473.0 10 5325.5 11 6383.0 12 7777.5 13 11192.5 14 21174.5 15 28904.5 16 39180.0 17 40479.0 18 33596.0 19 38429.0 20 47680.0 21 58989.5 22 73043.0 23 91814.5 24 114546.5 25 140219.5 26 168329.5 27 204105.5 28 246907.5 29 290579.0 30 335027.5 31 384895.0 32 443126.0 33 505112.5 34 571389.0 35 643532.5 36 723200.0 37 820135.5 38 918386.0 39 989623.0 40 1043279.0 41 1113428.0 42 1221444.0 43 1324631.5 44 1402643.0 45 1457888.0 46 1461692.0 47 1418219.0 48 1383969.0 49 1392773.0 50 1352468.0 51 1262378.5 52 1153566.0 53 1024788.5 54 932610.5 55 858835.0 56 772061.5 57 691009.5 58 634992.5 59 585073.5 60 523352.0 61 467057.0 62 417094.0 63 362881.5 64 312856.0 65 267083.0 66 223923.0 67 185961.0 68 152625.5 69 122417.5 70 95550.5 71 74161.5 72 57641.5 73 44810.0 74 34897.5 75 25441.0 76 17399.5 77 12592.0 78 9540.5 79 6228.5 80 3194.0 81 1537.0 82 862.5 83 558.5 84 391.5 85 312.5 86 254.5 87 179.0 88 135.0 89 120.0 90 90.5 91 65.5 92 51.0 93 35.0 94 20.5 95 14.5 96 12.0 97 9.5 98 5.0 99 1.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.04925231573376848 2 0.004100677283414422 3 0.02945594264142299 4 0.008012817680235077 5 4.418833279541403E-5 6 8.012817680235078E-4 7 9.574138772339706E-4 8 0.0019325030875861067 9 0.005535325154838865 10-11 0.005698821986181896 12-13 0.0020886351967965696 14-15 1.590779980634905E-4 16-17 8.307406565537838E-4 18-19 4.492480500867093E-4 20-21 7.158509912857072E-4 22-23 2.0621221971193213E-4 24-25 0.001791100422640782 26-27 0.0030313196297654024 28-29 0.003415758125085504 30-31 0.003971058173881208 32-33 0.0038370202310684517 34-35 2.710217744785394E-4 36-37 0.0014317019825714145 38-39 7.158509912857072E-4 40-41 9.633056549400257E-4 42-43 0.0018824229770846377 44-45 5.715024374873547E-4 46-47 5.656106597812996E-4 48-49 1.414026649453249E-4 50-51 4.6250454992533344E-4 52-53 1.2667322068018688E-4 54-55 8.572536562310322E-4 56-57 4.0358677286478145E-4 58-59 0.0017940463114938096 60-61 8.307406565537838E-4 62-63 6.333661034009344E-5 64-65 3.093183295678982E-5 66-67 2.945888853027602E-6 68-69 6.480955476660724E-5 70-71 0.0011547884303868198 72-73 2.4892760808083235E-4 74-75 2.945888853027602E-6 76-77 0.0016762107573727056 78-79 6.628249919312105E-4 80-81 6.480955476660724E-5 82-83 0.0034849865131316533 84-85 1.3845677609229727E-4 86-87 8.690372116431425E-5 88-89 7.364722132569005E-6 90-91 0.0012623133735223276 92-93 0.002835418021039067 94-95 3.5350666236331226E-5 96-97 8.425242119658941E-4 98-99 0.0012210709295799411 100 7.600393240811213E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 3.3945612E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 41.64831545553487 #Duplication Level Percentage of deduplicated Percentage of total 1 73.52565719529062 30.622197649449806 2 14.650597144236976 12.203453829502799 3 4.899200192410298 6.121303052799636 4 2.2236461058806203 3.7044445791675105 5 1.1752010448011327 2.447257191877587 6 0.7026902005524817 1.7559517884073692 7 0.4557135107076557 1.3285790035911198 8 0.3217964657824409 1.0721824575506667 9 0.22508784412368243 0.8437076583542449 >10 1.5007151830928667 12.486946892500871 >50 0.12514953472972323 3.51314198066057 >100 0.17505493484196766 17.594464662298634 >500 0.018744341285716108 4.868586934235714 >1k 6.036070807123163E-4 0.36561207721899824 >5k 3.567379582233033E-5 0.11515481091368919 >10k+ 1.0702138744415941E-4 0.95701543147074 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 41295 0.12165048018577482 No Hit ATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTT 36262 0.1068238215884869 No Hit GATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 34252 0.10090258499390141 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 1.472944426513801E-5 0.0 0.0 0.0 0.0 2 1.472944426513801E-5 0.0 0.0 0.0 0.0 3 1.472944426513801E-5 0.0 0.0 0.0 0.0 4 1.472944426513801E-5 0.0 2.945888853027602E-6 0.0 0.0 5 2.0621221971193214E-5 0.0 2.945888853027602E-6 0.0 0.0 6 2.0621221971193214E-5 0.0 2.945888853027602E-6 0.0 0.0 7 2.0621221971193214E-5 0.0 5.891777706055204E-6 0.0 0.0 8 2.3567110824220816E-5 0.0 5.891777706055204E-6 0.0 0.0 9 2.945888853027602E-5 0.0 5.891777706055204E-6 0.0 0.0 10-11 6.0390721487065834E-5 0.0 5.891777706055204E-6 0.0 0.0 12-13 1.7528038675514233E-4 2.945888853027602E-6 5.891777706055204E-6 0.0 0.0 14-15 3.7118199548147784E-4 2.945888853027602E-6 5.891777706055204E-6 0.0 0.0 16-17 6.628249919312104E-4 5.891777706055204E-6 5.891777706055204E-6 0.0 0.0 18-19 0.0011312213195625992 8.837666559082806E-6 5.891777706055204E-6 1.472944426513801E-6 0.0 20-21 0.0017513309231249094 8.837666559082806E-6 5.891777706055204E-6 2.945888853027602E-6 0.0 22-23 0.0027057989115058523 8.837666559082806E-6 5.891777706055204E-6 2.945888853027602E-6 2.945888853027602E-6 24-25 0.0037692647874488167 8.837666559082806E-6 5.891777706055204E-6 2.945888853027602E-6 2.945888853027602E-6 26-27 0.0051287924931210545 8.837666559082806E-6 5.891777706055204E-6 2.945888853027602E-6 2.945888853027602E-6 28-29 0.0064662260323955865 8.837666559082806E-6 5.891777706055204E-6 2.945888853027602E-6 2.945888853027602E-6 30-31 0.007501705964234788 8.837666559082806E-6 5.891777706055204E-6 2.945888853027602E-6 2.945888853027602E-6 32-33 0.008148328567474347 8.837666559082806E-6 5.891777706055204E-6 2.945888853027602E-6 2.945888853027602E-6 34-35 0.008737506338079869 8.837666559082806E-6 5.891777706055204E-6 7.364722132569006E-6 2.945888853027602E-6 36-37 0.009460722051498144 1.3256499838624209E-5 5.891777706055204E-6 1.0310610985596607E-5 2.945888853027602E-6 38-39 0.010307665096743579 1.472944426513801E-5 5.891777706055204E-6 1.1783555412110408E-5 2.945888853027602E-6 40-41 0.011161972864121582 1.472944426513801E-5 5.891777706055204E-6 1.1783555412110408E-5 2.945888853027602E-6 42-43 0.01224900585088877 1.472944426513801E-5 5.891777706055204E-6 1.1783555412110408E-5 2.945888853027602E-6 44-45 0.013677761944607156 1.472944426513801E-5 5.891777706055204E-6 1.1783555412110408E-5 2.945888853027602E-6 46-47 0.015848882029288497 1.472944426513801E-5 5.891777706055204E-6 1.1783555412110408E-5 2.945888853027602E-6 48-49 0.018993618379895466 1.472944426513801E-5 5.891777706055204E-6 1.1783555412110408E-5 2.945888853027602E-6 50-51 0.023303453771874844 1.472944426513801E-5 8.837666559082806E-6 1.1783555412110408E-5 2.945888853027602E-6 52-53 0.029093598312500597 1.472944426513801E-5 8.837666559082806E-6 1.1783555412110408E-5 2.945888853027602E-6 54-55 0.03642149683440676 1.472944426513801E-5 8.837666559082806E-6 1.1783555412110408E-5 2.945888853027602E-6 56-57 0.04598090616248132 1.472944426513801E-5 8.837666559082806E-6 1.3256499838624209E-5 2.945888853027602E-6 58-59 0.05850093378784863 1.472944426513801E-5 8.837666559082806E-6 1.472944426513801E-5 2.945888853027602E-6 60-61 0.0746664988688376 1.472944426513801E-5 8.837666559082806E-6 1.472944426513801E-5 4.418833279541403E-6 62-63 0.09509181923130447 1.472944426513801E-5 8.837666559082806E-6 1.7675333118165613E-5 7.364722132569006E-6 64-65 0.1212866629124259 1.472944426513801E-5 8.837666559082806E-6 1.7675333118165613E-5 8.837666559082806E-6 66-67 0.15462086822885973 1.472944426513801E-5 8.837666559082806E-6 1.7675333118165613E-5 8.837666559082806E-6 68-69 0.1963479108875692 1.472944426513801E-5 8.837666559082806E-6 1.7675333118165613E-5 1.1783555412110408E-5 70-71 0.2476181604856616 1.472944426513801E-5 8.837666559082806E-6 2.0621221971193214E-5 1.1783555412110408E-5 72-73 0.31068227610684995 1.620238869165181E-5 8.837666559082806E-6 2.3567110824220816E-5 1.1783555412110408E-5 74-75 0.38663760134888714 1.7675333118165613E-5 8.837666559082806E-6 2.3567110824220816E-5 1.1783555412110408E-5 76-77 0.4789617580027722 1.7675333118165613E-5 8.837666559082806E-6 2.3567110824220816E-5 1.472944426513801E-5 78-79 0.5893766181030997 1.9148277544679412E-5 8.837666559082806E-6 2.3567110824220816E-5 1.472944426513801E-5 80-81 0.7195966889623319 2.0621221971193214E-5 1.0310610985596607E-5 2.5040055250734615E-5 1.472944426513801E-5 82-83 0.8731143218157328 2.0621221971193214E-5 1.1783555412110408E-5 2.6512999677248417E-5 1.472944426513801E-5 84-85 1.052502750576422 2.3567110824220816E-5 1.1783555412110408E-5 2.798594410376222E-5 1.472944426513801E-5 86-87 1.2602468324919287 2.3567110824220816E-5 1.1783555412110408E-5 2.945888853027602E-5 1.472944426513801E-5 88 1.4338465896564185 2.3567110824220816E-5 1.1783555412110408E-5 2.945888853027602E-5 1.472944426513801E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ATCTCGA 24380 0.0 47.234802 1 TCTCGAG 29835 0.0 38.420998 2 CTCGAGG 36080 0.0 31.679394 3 TCGAGGG 41795 0.0 27.29527 4 TATCTCG 12495 0.0 25.249176 2 GATCCGG 32675 0.0 23.351763 1 ATCCGGC 38630 0.0 19.766161 2 CGAGGGC 58670 0.0 19.579062 5 AGGGCGC 60955 0.0 18.683182 7 ATATCTC 17930 0.0 18.208055 1 GGGCGCG 64495 0.0 17.752438 8 GAGGGCG 69465 0.0 16.52967 6 GGATCCG 23170 0.0 16.315145 2 TCCGGCG 46940 0.0 16.276787 3 GCGCCGT 51480 0.0 14.589655 9 CGGCGCG 52810 0.0 14.471419 5 TATGGAT 22945 0.0 14.262056 2 CGTCGAC 49495 0.0 13.028041 12-13 GGCGCGC 113865 0.0 12.936461 9 CCGGCGC 59600 0.0 12.934199 4 >>END_MODULE