FastQCFastQC Report
Wed 25 May 2016
SRR491073_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR491073_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32280516
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTT388480.12034504033330817No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCTCGA244600.051.8142621
TCTCGAG316400.040.139262
CTCGAGG377900.033.4450873
TCGAGGG457550.027.6148154
GATCCGG410600.023.7971921
TATGGAT176050.022.3547952
ATCCGGC483250.020.1409422
AGGGCGC652400.019.3724787
TATCTCG133550.019.2938082
CGAGGGC700250.018.2232175
GGGCGCG697850.018.063638
GGATCCG286350.017.3892172
ATGGATC289100.017.0863511
TCCGGCG589550.016.772533
GAGGGCG803250.015.968396
TGGATCC320750.015.9137011
ATATCTC183200.014.4317491
GCGCCGT697600.013.9938879
CGGCGCG708650.013.8420065
CCGGCGC756100.013.1735594
Produced by FastQC (version 0.11.5)