##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR491073_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 32280516 Sequences flagged as poor quality 0 Sequence length 100 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.47119076411294 33.0 31.0 34.0 30.0 34.0 2 31.747096174051244 34.0 31.0 34.0 30.0 34.0 3 31.806804296436898 34.0 31.0 34.0 30.0 34.0 4 35.23196134782975 37.0 35.0 37.0 33.0 37.0 5 34.9992333455884 37.0 35.0 37.0 32.0 37.0 6 34.95925786935996 37.0 35.0 37.0 32.0 37.0 7 34.8766178644728 37.0 35.0 37.0 32.0 37.0 8 34.868423974387525 37.0 35.0 37.0 32.0 37.0 9 36.486444392648494 39.0 37.0 39.0 32.0 39.0 10-11 36.47903995710601 39.0 37.0 39.0 32.0 39.0 12-13 36.44817195611124 39.0 37.0 39.0 32.0 39.0 14-15 37.721265840360175 40.0 38.0 41.0 32.0 41.0 16-17 37.67740390209376 40.0 38.0 41.0 32.0 41.0 18-19 37.65775488223298 40.0 38.0 41.0 32.0 41.0 20-21 37.623255402732724 40.0 38.0 41.0 32.0 41.0 22-23 37.54338276376995 40.0 38.0 41.0 32.0 41.0 24-25 37.47473101111519 40.0 38.0 41.0 32.0 41.0 26-27 37.271630214956915 40.0 37.5 41.0 31.5 41.0 28-29 37.09081854515585 40.0 37.0 41.0 31.0 41.0 30-31 36.92188349777308 40.0 37.0 41.0 31.0 41.0 32-33 36.76560684469852 40.0 36.0 41.0 30.0 41.0 34-35 36.62288683675317 40.0 36.0 41.0 30.0 41.0 36-37 36.45495521199227 40.0 36.0 41.0 30.0 41.0 38-39 36.263866181693004 40.0 35.0 41.0 30.0 41.0 40-41 35.97817576707882 40.0 35.0 41.0 28.5 41.0 42-43 35.64638305348031 39.5 35.0 41.0 27.5 41.0 44-45 35.275876940752745 39.0 35.0 41.0 25.5 41.0 46-47 34.87492318896018 39.0 35.0 41.0 23.5 41.0 48-49 34.533231516497445 39.0 34.5 41.0 22.0 41.0 50-51 34.25684772201287 38.5 34.0 40.0 20.5 41.0 52-53 34.04890989041191 38.0 34.0 40.0 20.0 41.0 54-55 33.895152047755374 38.0 34.0 40.0 18.5 41.0 56-57 33.6245298247401 38.0 33.5 40.0 17.5 41.0 58-59 33.33309766485765 37.0 33.0 40.0 14.5 41.0 60-61 32.98507593558914 37.0 33.0 40.0 13.0 41.0 62-63 32.612907333947206 36.0 33.0 39.5 9.5 41.0 64-65 32.24513889431012 35.5 32.0 39.0 7.5 41.0 66-67 31.94784654309739 35.0 32.0 39.0 7.0 40.0 68-69 31.803304755103667 35.0 32.0 38.0 6.0 40.0 70-71 31.50286528877048 35.0 32.0 37.0 2.0 39.5 72-73 31.15173217801103 35.0 32.0 37.0 2.0 39.0 74-75 30.835845142624116 35.0 32.0 36.0 2.0 39.0 76-77 30.18221166291146 34.5 31.0 35.0 2.0 37.0 78-79 30.275964981476754 35.0 31.0 35.0 2.0 37.0 80-81 30.108421733407233 35.0 31.5 35.0 2.0 36.5 82-83 29.810302242380512 35.0 31.0 35.0 2.0 36.0 84-85 29.64706865280592 35.0 31.0 35.0 2.0 36.0 86-87 29.446874393209825 35.0 31.0 35.0 2.0 36.0 88-89 29.252669195250782 35.0 31.0 35.0 2.0 35.0 90-91 29.085071580020593 35.0 31.0 35.0 2.0 35.0 92-93 28.897079309388985 35.0 30.0 35.0 2.0 35.0 94-95 28.683859173750506 34.0 30.0 35.0 2.0 35.0 96-97 28.45210689940644 34.0 30.0 35.0 2.0 35.0 98-99 28.152012470928284 34.0 29.5 35.0 2.0 35.0 100 27.50340988973039 34.0 29.0 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.37632751964827094 1101 2 0.47968784452484314 1101 3 0.4851778486899434 1101 4 0.37848013488343923 1101 5 0.4782754596199865 1101 6 0.4256188845280846 1101 7 0.5356460444508784 1101 8 0.4852602293161965 1101 9 0.6259342947460524 1101 10-11 0.6206803507496303 1101 12-13 0.6302085652314418 1101 14-15 0.6125319242024645 1101 16-17 0.6771482225386976 1101 18-19 0.7533732644178386 1101 20-21 0.7537761320211587 1101 22-23 0.767129959936959 1101 24-25 0.7715231658351982 1101 26-27 0.7623506615356419 1101 28-29 0.7784773436368724 1101 30-31 0.7635087133983447 1101 32-33 0.677508482129511 1101 34-35 0.7248180663357999 1101 36-37 0.7601064962898505 1101 38-39 0.6938497840250619 1101 40-41 0.8282152439837986 1101 42-43 0.8132886384598095 1101 44-45 0.7888559670303792 1101 46-47 0.8224771467258307 1101 48-49 0.84124232804146 1101 50-51 0.8198531302628069 1101 52-53 0.8644282502808522 1101 54-55 0.8238066308651142 1101 56-57 0.8678249917899237 1101 58-59 0.7990082778573111 1101 60-61 0.7919404356967235 1101 62-63 0.8500170124166857 1101 64-65 0.8156448148776079 1101 66-67 0.7576882918571641 1101 68-69 0.8196509885906949 1101 70-71 0.8356622494566324 1101 72-73 0.8140725910852318 1101 74-75 0.8185138923484878 1101 76-77 0.8291758397641047 1101 78-79 0.7864368757272686 1101 80-81 0.841414729346802 1101 82-83 0.9202773850630166 1101 84-85 0.8951661796239883 1101 86-87 0.8733964262789335 1101 88-89 0.8527940492022239 1101 90-91 0.8387800364472433 1101 92-93 0.9426421429395546 1101 94-95 0.868422990231636 1101 96-97 0.9104109604626665 1101 98-99 0.915458205067182 1101 100 0.9149273587763531 1102 1 0.2367122754846669 1102 2 0.22886160640483055 1102 3 0.30378242515060094 1102 4 0.3132438032107103 1102 5 0.3465125952242687 1102 6 0.36288111117290356 1102 7 0.38593162907391587 1102 8 0.37681494547824457 1102 9 0.4918942900048009 1102 10-11 0.36214481170087254 1102 12-13 0.3696273654084763 1102 14-15 0.431091199207593 1102 16-17 0.4426892039847985 1102 18-19 0.445434240274011 1102 20-21 0.5076202393713984 1102 22-23 0.40543311325684783 1102 24-25 0.3923125738533173 1102 26-27 0.47227410251459645 1102 28-29 0.4479214941532419 1102 30-31 0.47275432350952684 1102 32-33 0.4959419537875718 1102 34-35 0.503323855878115 1102 36-37 0.46164967617757213 1102 38-39 0.48939858449753615 1102 40-41 0.5158709695431867 1102 42-43 0.49915248518387045 1102 44-45 0.4478657358612068 1102 46-47 0.5676823193763454 1102 48-49 0.5363291727486015 1102 50-51 0.544489198364225 1102 52-53 0.44975149905292966 1102 54-55 0.43744026807964786 1102 56-57 0.5165184693051188 1102 58-59 0.440270908420203 1102 60-61 0.5485381158528639 1102 62-63 0.521400578298433 1102 64-65 0.5086670544714522 1102 66-67 0.518818497581691 1102 68-69 0.645186141037378 1102 70-71 0.5870712821010358 1102 72-73 0.5709206073928534 1102 74-75 0.5292741024323 1102 76-77 0.5454382650834155 1102 78-79 0.5746671514284571 1102 80-81 0.5882691659386907 1102 82-83 0.6156523268855665 1102 84-85 0.560233679272546 1102 86-87 0.5542992731001171 1102 88-89 0.549438201632455 1102 90-91 0.5270637968050025 1102 92-93 0.5839294651609102 1102 94-95 0.5942128272144274 1102 96-97 0.5743975416825151 1102 98-99 0.6439809501203015 1102 100 0.621845116889876 1103 1 -0.03631710654648401 1103 2 0.009777054099661342 1103 3 0.028084769776089757 1103 4 0.07385650834339685 1103 5 0.07920863571420256 1103 6 0.10416452456678371 1103 7 0.15220640155136778 1103 8 0.10980716734865581 1103 9 0.06509618494995806 1103 10-11 0.05036511199184446 1103 12-13 0.09245594507315502 1103 14-15 -0.038942020235943176 1103 16-17 0.05646215921331077 1103 18-19 0.15488429190920527 1103 20-21 0.07104712810330938 1103 22-23 0.009145935179965647 1103 24-25 0.009693978437539386 1103 26-27 0.018393377323086213 1103 28-29 0.05417062307421361 1103 30-31 0.14958115959760931 1103 32-33 0.12757091414864163 1103 34-35 -0.010866585337112156 1103 36-37 0.07322725254373808 1103 38-39 0.03395067844581234 1103 40-41 0.07837192287402672 1103 42-43 0.03861276301791605 1103 44-45 0.10733126623228628 1103 46-47 0.010681442357025617 1103 48-49 -0.05982401686764405 1103 50-51 -0.060733891891779024 1103 52-53 -0.06879616278586553 1103 54-55 -0.028792311870141418 1103 56-57 0.04579589661206995 1103 58-59 0.02728544932267596 1103 60-61 0.10644973582645889 1103 62-63 0.10787069970820795 1103 64-65 0.1810404648409687 1103 66-67 0.03548880113630304 1103 68-69 0.012782632314390696 1103 70-71 0.12419693765300366 1103 72-73 0.0826090545052871 1103 74-75 0.13788110323318747 1103 76-77 0.10509698749357099 1103 78-79 0.11243059083017215 1103 80-81 0.1680782277929609 1103 82-83 0.21831742956069888 1103 84-85 0.1990400280884046 1103 86-87 0.20981239221656622 1103 88-89 0.2571885069002846 1103 90-91 0.23719164320173647 1103 92-93 0.08074324177033176 1103 94-95 0.14116185167584305 1103 96-97 0.04081651569016387 1103 98-99 0.17948658280302965 1103 100 0.23692736019715 1104 1 -0.160734717379448 1104 2 -0.19317466220876867 1104 3 -0.1004237302614186 1104 4 -0.11880070524887287 1104 5 -0.08170670619070108 1104 6 -0.04651309851428209 1104 7 -0.03703811332979967 1104 8 -0.04128469283406844 1104 9 -0.06608578263521281 1104 10-11 -0.07181753534545265 1104 12-13 -0.13204833872440958 1104 14-15 -0.1915929228070965 1104 16-17 -0.19520095700535478 1104 18-19 -0.09713822159734065 1104 20-21 -0.18213071836182593 1104 22-23 -0.1287884683631475 1104 24-25 -0.08584498688411912 1104 26-27 -0.1142881458478584 1104 28-29 -0.10634916815889284 1104 30-31 -0.07639575323797487 1104 32-33 -0.13637346525708693 1104 34-35 -0.13354397519194094 1104 36-37 -0.15751141470921937 1104 38-39 -0.12927547998408784 1104 40-41 -0.10216217036324338 1104 42-43 -0.12651741583647436 1104 44-45 -0.1065005863469537 1104 46-47 -0.1705082259751407 1104 48-49 -0.07775112088092584 1104 50-51 -0.10683340903464256 1104 52-53 -0.09046684341457478 1104 54-55 -0.1236316881426589 1104 56-57 -0.08896110425875037 1104 58-59 -0.014321975015768373 1104 60-61 0.023279717071780226 1104 62-63 0.019215062506006575 1104 64-65 -0.06571448955903492 1104 66-67 0.01871132054394309 1104 68-69 -0.00860220715466653 1104 70-71 -0.07899184525782132 1104 72-73 -0.1365379975712493 1104 74-75 -0.09489773037044458 1104 76-77 -0.08826022708133507 1104 78-79 -0.06314706840835882 1104 80-81 -0.06802407333042382 1104 82-83 0.0648181026460648 1104 84-85 0.09189981145367554 1104 86-87 0.06647598205999827 1104 88-89 0.05892123000438332 1104 90-91 1.564764894368409E-5 1104 92-93 0.060394153298961584 1104 94-95 0.0042379403274495075 1104 96-97 -0.059142135350658265 1104 98-99 -0.020080348586919428 1104 100 -0.05792805343983787 1105 1 -0.3535005299615044 1105 2 -0.33150292813751747 1105 3 -0.2890299122398048 1105 4 -0.18876425214779857 1105 5 -0.2808776247633844 1105 6 -0.2773814243215895 1105 7 -0.3162034151445354 1105 8 -0.27391724601439904 1105 9 -0.3351351929234738 1105 10-11 -0.35841851207273834 1105 12-13 -0.35738298376223554 1105 14-15 -0.5216911249326657 1105 16-17 -0.4862974590510305 1105 18-19 -0.4843826700904046 1105 20-21 -0.4944669138744118 1105 22-23 -0.45054229611717744 1105 24-25 -0.43083915958399643 1105 26-27 -0.46448852758798154 1105 28-29 -0.5089685641963158 1105 30-31 -0.5106235063666915 1105 32-33 -0.5567677365378856 1105 34-35 -0.47122824188664936 1105 36-37 -0.4380001351158995 1105 38-39 -0.4283483879444674 1105 40-41 -0.414038215664732 1105 42-43 -0.46938526644755285 1105 44-45 -0.45246145404840377 1105 46-47 -0.4295362735765451 1105 48-49 -0.4237881895693576 1105 50-51 -0.49745967832468097 1105 52-53 -0.468720330295028 1105 54-55 -0.4701439567649217 1105 56-57 -0.44016538105147873 1105 58-59 -0.45771417098718814 1105 60-61 -0.46896773294562877 1105 62-63 -0.43909489177993066 1105 64-65 -0.43091318722136407 1105 66-67 -0.37627723083549824 1105 68-69 -0.35585842672129075 1105 70-71 -0.36286649826568507 1105 72-73 -0.41720330299888175 1105 74-75 -0.4262626807073424 1105 76-77 -0.3778342079611079 1105 78-79 -0.3634450955761963 1105 80-81 -0.4219691022507419 1105 82-83 -0.2512760630713444 1105 84-85 -0.3044914308388158 1105 86-87 -0.3618042892281288 1105 88-89 -0.28575634118933024 1105 90-91 -0.31590729304488363 1105 92-93 -0.297046222646582 1105 94-95 -0.3059767014538757 1105 96-97 -0.2931683295799594 1105 98-99 -0.27439736930051595 1105 100 -0.33148667187718317 1106 1 -0.5729106865068481 1106 2 -0.6299067902300415 1106 3 -0.5335983596310427 1106 4 -0.4430395828390985 1106 5 -0.48410646285375947 1106 6 -0.4781883340854023 1106 7 -0.47413904677932095 1106 8 -0.516522160697086 1106 9 -0.5789684990549375 1106 10-11 -0.6357740402381182 1106 12-13 -0.6584778818924946 1106 14-15 -0.7664211824696778 1106 16-17 -0.7660983260663272 1106 18-19 -0.7731853329228144 1106 20-21 -0.7064116252522084 1106 22-23 -0.7914093718688875 1106 24-25 -0.8984931523563731 1106 26-27 -0.8315548786304063 1106 28-29 -0.8070716050497424 1106 30-31 -0.843210236182486 1106 32-33 -0.8719253820981194 1106 34-35 -0.8641675279279752 1106 36-37 -0.8549452359069605 1106 38-39 -0.9109180113775182 1106 40-41 -0.846206820932025 1106 42-43 -0.911344563037467 1106 44-45 -0.8807186493395918 1106 46-47 -0.905034041898233 1106 48-49 -0.9032567184868867 1106 50-51 -0.9206968128235289 1106 52-53 -0.9134037401861264 1106 54-55 -0.863139781176713 1106 56-57 -0.8221760202199917 1106 58-59 -0.7710530542789584 1106 60-61 -0.7946571354467267 1106 62-63 -0.7811325784775534 1106 64-65 -0.7600126356878718 1106 66-67 -0.7056191974078416 1106 68-69 -0.7474019126617293 1106 70-71 -0.7297578962040205 1106 72-73 -0.7447645439907653 1106 74-75 -0.7715604890621499 1106 76-77 -0.8207712630606032 1106 78-79 -0.7291397180683852 1106 80-81 -0.6981689529811916 1106 82-83 -0.6189694536368968 1106 84-85 -0.6422418351398598 1106 86-87 -0.7219597225422625 1106 88-89 -0.6677175700346574 1106 90-91 -0.683631183939827 1106 92-93 -0.6537808095741688 1106 94-95 -0.709178755697657 1106 96-97 -0.6716587602680306 1106 98-99 -0.6599102383020607 1106 100 -0.7355850847949625 1107 1 -0.8262681712599687 1107 2 -0.8494702174628479 1107 3 -0.7718103275568211 1107 4 -0.6955438795292537 1107 5 -0.7849299066846029 1107 6 -0.7990784482870623 1107 7 -0.8295254727474841 1107 8 -0.8280107157052328 1107 9 -1.0124655403952545 1107 10-11 -1.0272246574187704 1107 12-13 -1.0173450592401991 1107 14-15 -1.21812612155869 1107 16-17 -1.2854641186904487 1107 18-19 -1.364969305922891 1107 20-21 -1.3041255715384708 1107 22-23 -1.2334581445634925 1107 24-25 -1.2189102752148528 1107 26-27 -1.2320286662125923 1107 28-29 -1.3186827573359992 1107 30-31 -1.3109993804217694 1107 32-33 -1.3253397490338443 1107 34-35 -1.3300151160674432 1107 36-37 -1.3598417163513545 1107 38-39 -1.4052619699492723 1107 40-41 -1.4259980998848931 1107 42-43 -1.451734905512815 1107 44-45 -1.4278580769371132 1107 46-47 -1.2959119567773314 1107 48-49 -1.2692383703276207 1107 50-51 -1.2466981836869593 1107 52-53 -1.3025610246222215 1107 54-55 -1.1945514725802155 1107 56-57 -1.2016397290395986 1107 58-59 -1.229479647369331 1107 60-61 -1.2477660088477975 1107 62-63 -1.1965804757882665 1107 64-65 -1.1486298062561886 1107 66-67 -1.1680673935619623 1107 68-69 -1.2297747478083068 1107 70-71 -1.2322085733881032 1107 72-73 -1.2226890017516077 1107 74-75 -1.1615120517713287 1107 76-77 -1.2243487524557004 1107 78-79 -1.1691194721850238 1107 80-81 -1.192835597188207 1107 82-83 -1.1250166604265246 1107 84-85 -1.1309987956156 1107 86-87 -1.1647760120018056 1107 88-89 -1.177906672514215 1107 90-91 -1.204376249408572 1107 92-93 -1.233873603774601 1107 94-95 -1.2633761425311434 1107 96-97 -1.3032633535223184 1107 98-99 -1.3459539358771409 1107 100 -1.4085316339847616 1108 1 -1.422353011011893 1108 2 -1.3703815447697352 1108 3 -1.3401901647572672 1108 4 -1.1986645736919286 1108 5 -1.374013749684714 1108 6 -1.4767919624177637 1108 7 -1.739714624945286 1108 8 -1.588223802647164 1108 9 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0.798147061966521 2305 88-89 0.8326178320059654 2305 90-91 0.8268827389305464 2305 92-93 0.8143736593521176 2305 94-95 0.9071782704959759 2305 96-97 0.9588245253694829 2305 98-99 0.9778702185955481 2305 100 1.022069652177894 2306 1 0.26494690293090173 2306 2 0.05202903248117963 2306 3 0.14570257314914414 2306 4 0.1831709739615448 2306 5 0.2592281229926101 2306 6 0.2646690103743694 2306 7 0.34308242213135287 2306 8 0.28380381600245386 2306 9 0.3667736730023279 2306 10-11 0.3034693219787954 2306 12-13 0.2972552359055953 2306 14-15 0.36514956916258967 2306 16-17 0.4065022427840006 2306 18-19 0.39824982989065916 2306 20-21 0.3603173227699088 2306 22-23 0.3817037267686132 2306 24-25 0.3968382297973818 2306 26-27 0.4182245952412913 2306 28-29 0.42865114675940674 2306 30-31 0.43656226077978744 2306 32-33 0.43650217643769196 2306 34-35 0.48595715389701866 2306 36-37 0.36092007255503233 2306 38-39 0.38378885275581354 2306 40-41 0.4191991002113298 2306 42-43 0.4224531360163368 2306 44-45 0.4523108914430267 2306 46-47 0.46600643523697727 2306 48-49 0.4353539368993822 2306 50-51 0.46117681247931586 2306 52-53 0.5117242067830077 2306 54-55 0.5161033103676047 2306 56-57 0.5352934259660671 2306 58-59 0.5310504552090265 2306 60-61 0.5221197673416214 2306 62-63 0.5669876854397984 2306 64-65 0.5854380352585551 2306 66-67 0.5514534536460971 2306 68-69 0.4980317357465296 2306 70-71 0.5107879201863312 2306 72-73 0.4987294023304578 2306 74-75 0.494227843903122 2306 76-77 0.5036396119697812 2306 78-79 0.47280857668236465 2306 80-81 0.4504798485228747 2306 82-83 0.49548293594437 2306 84-85 0.47929341357302846 2306 86-87 0.4851424631343981 2306 88-89 0.5718970208925569 2306 90-91 0.5498567657242361 2306 92-93 0.488273732564366 2306 94-95 0.6001590766997182 2306 96-97 0.6531714128152935 2306 98-99 0.6750452100213202 2306 100 0.711996766977439 2307 1 -0.2918906413472584 2307 2 -0.178731029297321 2307 3 -0.1555402829592829 2307 4 -0.10378314102035802 2307 5 -0.07816278408896693 2307 6 -0.08130640688212765 2307 7 -0.0020483925310657014 2307 8 -0.06140148915490329 2307 9 -0.052572235352748464 2307 10-11 -0.1196741973038229 2307 12-13 -0.1351004879123323 2307 14-15 -0.39657993316832574 2307 16-17 -0.19070032789274194 2307 18-19 -0.19381489907710403 2307 20-21 -0.2953451559287785 2307 22-23 -0.266057569963273 2307 24-25 -0.2278745962271529 2307 26-27 -0.2522939099757835 2307 28-29 -0.24520334954038248 2307 30-31 -0.29871223440679984 2307 32-33 -0.2812069291600636 2307 34-35 -0.22970456382508075 2307 36-37 -0.31061510962976513 2307 38-39 -0.3106935594130036 2307 40-41 -0.2867946029911934 2307 42-43 -0.25909652414018325 2307 44-45 -0.25507644921466976 2307 46-47 -0.18402847616695794 2307 48-49 -0.20190998489746192 2307 50-51 -0.23401574082173937 2307 52-53 -0.21944763452792415 2307 54-55 -0.18347414526355266 2307 56-57 -0.16202118180932956 2307 58-59 -0.15497057039581819 2307 60-61 -0.45183160869977 2307 62-63 -0.33239362646491344 2307 64-65 -0.23404191493127513 2307 66-67 -0.2518939863683478 2307 68-69 -0.27866498889789426 2307 70-71 -0.31806092015439447 2307 72-73 -0.3143985884017617 2307 74-75 -0.20614355983383703 2307 76-77 -0.16324974448253826 2307 78-79 -0.2048211097941568 2307 80-81 -0.24458450958777078 2307 82-83 -0.21708037305687355 2307 84-85 -0.2450311507927445 2307 86-87 -0.20996526703627794 2307 88-89 -0.1922525569410567 2307 90-91 -0.23143640214054528 2307 92-93 -0.24044069681842117 2307 94-95 -0.22780160367851465 2307 96-97 -0.19376075750723132 2307 98-99 -0.10255024567314308 2307 100 -0.1023865470010854 2308 1 -0.6205824400723863 2308 2 -0.590820623315981 2308 3 -0.602773650312713 2308 4 -0.585977483571213 2308 5 -0.7184676117202571 2308 6 -0.7908028497856492 2308 7 -0.7790696979809724 2308 8 -0.7791596077257879 2308 9 -0.8899760819760019 2308 10-11 -0.9450142678918425 2308 12-13 -1.0980445657513869 2308 14-15 -1.364028374018467 2308 16-17 -1.3354302957727953 2308 18-19 -1.3513524665941148 2308 20-21 -1.2593461374738837 2308 22-23 -1.3251267359954468 2308 24-25 -1.2681015756754306 2308 26-27 -1.369857027941272 2308 28-29 -1.3676184151640385 2308 30-31 -1.2946186062127012 2308 32-33 -1.2742147056639936 2308 34-35 -1.239004824486436 2308 36-37 -1.2402424632818096 2308 38-39 -1.1864160410055646 2308 40-41 -1.1918653970131672 2308 42-43 -1.173741502149234 2308 44-45 -1.3103613973475419 2308 46-47 -1.5803193635169066 2308 48-49 -1.3343714140362408 2308 50-51 -1.461269968275495 2308 52-53 -1.4674414952765318 2308 54-55 -1.5341909649995316 2308 56-57 -1.5757815660471053 2308 58-59 -1.6019930941691243 2308 60-61 -1.6368475504463937 2308 62-63 -1.5068679520980304 2308 64-65 -1.7039239378387592 2308 66-67 -1.816871103380553 2308 68-69 -1.6860734242575006 2308 70-71 -1.6567581105763978 2308 72-73 -1.6130815071201603 2308 74-75 -1.6146203779190778 2308 76-77 -1.595976429765166 2308 78-79 -1.580963567637756 2308 80-81 -1.634915171897788 2308 82-83 -2.7744931051745 2308 84-85 -2.092655325837253 2308 86-87 -1.8039757952381414 2308 88-89 -1.7399021329021522 2308 90-91 -1.8237717132165479 2308 92-93 -1.7806978648461005 2308 94-95 -1.8287567401501938 2308 96-97 -1.8894830754858951 2308 98-99 -1.7292565177695707 2308 100 -1.6298674887060933 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 467055.0 3 145091.0 4 85398.0 5 68619.0 6 65111.0 7 71431.0 8 79128.0 9 87487.0 10 95787.0 11 97835.0 12 101552.0 13 124879.0 14 181972.0 15 279577.0 16 359413.0 17 340596.0 18 260168.0 19 171635.0 20 136388.0 21 130000.0 22 138015.0 23 155011.0 24 183957.0 25 220207.0 26 265074.0 27 319877.0 28 384287.0 29 463059.0 30 566472.0 31 701621.0 32 885627.0 33 1153290.0 34 1603264.0 35 2451035.0 36 4405726.0 37 7489387.0 38 6633823.0 39 908454.0 40 3208.0 >>END_MODULE >>Per base sequence content pass #Base G A T C 1 22.157672086598993 26.87865932369185 26.642658156966796 24.321010432742362 2 24.81117836472719 26.523315057340437 25.810201263106762 22.85530531482561 3 24.592021920379594 25.289298674406297 25.58998064328644 24.52869876192767 4 25.13885393194655 24.76041430823485 25.701884799845736 24.398846959972868 5 25.700860882516647 24.948629169969873 25.409263598597775 23.9412463489157 6 25.626707560552724 24.94155818246164 24.706276040918006 24.725458216067626 7 25.650573686134976 25.270579093209395 25.00587903946393 24.0729681811917 8 25.302122854997044 25.03091977784911 25.162594675797497 24.50436269135635 9 24.853192992200455 24.71664764282681 25.381324900076436 25.048834464896295 10-11 24.897307271375567 24.975480380454957 25.233867561942525 24.893344786226947 12-13 24.499377251739 25.121513669234492 25.420503777382397 24.958605301644116 14-15 24.281985996368107 25.415349801225428 25.810171286075455 24.492492916331006 16-17 24.016102873608386 25.308020485824738 26.25088998393653 24.42498665663034 18-19 23.51683789521574 25.301880359122475 27.02972849087678 24.151553254785004 20-21 23.1277327241647 24.959471884500832 27.940629602299378 23.97216578903509 22-23 23.162076626668373 24.903223454081814 28.438248765633293 23.496451153616512 24-25 23.058309926724537 24.727997015833573 28.96482684272695 23.24886621471494 26-27 23.071702872629892 24.762511684393836 29.04468073788893 23.12110470508734 28-29 23.0960726423307 24.85564164603903 29.014315942091844 23.033969769538427 30-31 23.04900914106868 24.778998389986484 29.011372267536544 23.160620201408292 32-33 23.102971589830325 24.865034511562865 28.878798521475396 23.153195377131414 34-35 23.207455157568855 24.859313854081694 28.89700686150875 23.0362241268407 36-37 23.19541682343398 24.863006410046903 28.819845355275646 23.121731411243474 38-39 23.21615241311849 24.93924421940284 28.668886138239664 23.175717229239005 40-41 23.308131108241355 25.08841710245312 28.42615884768609 23.177292941619434 42-43 23.445869256654163 25.15480592016172 27.998231395791638 23.401093427392468 44-45 23.555477702627915 25.399516094041314 27.519275802140125 23.525730401190646 46-47 23.87972591307322 25.55649162785813 26.941215175709498 23.622567283359153 48-49 23.991103390978346 25.630498133092512 26.491271527118215 23.887126948810934 50-51 23.96592022143111 25.71847452732477 26.281177085716763 24.03442816552736 52-53 24.08132898760321 25.815265454324997 26.091079696713376 24.01232586135841 54-55 24.094820674020077 25.788398607327178 26.073267445465014 24.04351327318773 56-57 24.098070484423875 25.85025783178121 26.03593326910934 24.015738414685572 58-59 24.19537763915854 25.87496161085239 25.95901616655742 23.970644583431646 60-61 24.12396436015739 25.8499592551649 25.934766752276516 24.0913096324012 62-63 24.147396995293235 25.955288206986054 25.89105274214773 24.00626205557298 64-65 24.19457302487413 26.063584480618275 25.808157726988444 23.93368476751915 66-67 24.194782453210824 26.07536425494816 25.740536888266234 23.989316403574783 68-69 24.165608313355065 26.18361342870445 25.70472978835856 23.946048469581925 70-71 24.2503528468837 26.295466845171767 25.618437942094463 23.83574236585007 72-73 24.174353154363548 26.30645637085845 25.570852160223218 23.948338314554782 74-75 24.156565100596524 26.485657469926633 25.542528140312015 23.815249289164832 76-77 24.09472921500917 26.598312664532838 25.46763903983968 23.839319080618314 78-79 24.07266771892632 26.705039277271382 25.393108447412676 23.829184556389627 80-81 24.06875690606965 26.795331829163267 25.330403224165092 23.805508040601996 82-83 24.091607974784644 26.885798516298966 25.280350162366933 23.742243346549465 84-85 23.97920355403064 26.911767673899035 25.2681923789745 23.84083639309582 86-87 24.009290763394777 27.026685426216595 25.207884741994363 23.756139068394265 88-89 24.08274715011838 27.119726974552677 25.027853109186715 23.76967276614223 90-91 24.037211879487895 27.150282603350767 24.989713045033003 23.822792472128334 92-93 24.03092260536439 27.292690973658 24.867107647363547 23.809278773614064 94-95 24.097786776955818 27.362326314922402 24.798941180519655 23.74094572760212 96-97 24.046611426372273 27.470423658483366 24.605965023663767 23.876999891480587 98-99 24.120534705741537 27.564766694916877 24.459100407303318 23.855598192038276 100 24.12823459450926 27.797828913560878 24.13741668123698 23.93651981069288 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 4370.0 1 2845.0 2 993.0 3 606.5 4 529.0 5 544.0 6 614.0 7 712.0 8 843.5 9 1006.5 10 1238.0 11 1629.0 12 2086.0 13 3173.0 14 6373.5 15 9454.0 16 14406.5 17 16392.0 18 14985.5 19 17659.5 20 29634.0 21 36586.5 22 36793.0 23 42951.5 24 48557.0 25 55066.0 26 64409.5 27 77513.5 28 94150.5 29 109887.0 30 126977.0 31 155365.0 32 198707.0 33 251782.0 34 308728.5 35 371407.0 36 453862.5 37 572405.0 38 719377.0 39 837931.0 40 910111.5 41 997216.5 42 1153250.5 43 1327878.5 44 1472053.5 45 1581445.5 46 1631985.5 47 1632304.0 48 1659995.5 49 1749209.0 50 1657057.5 51 1447425.5 52 1262917.5 53 1053455.5 54 910472.5 55 826115.0 56 749652.5 57 669015.0 58 621107.5 59 584348.5 60 529996.0 61 486701.5 62 445941.0 63 396717.5 64 351231.0 65 301371.5 66 249376.0 67 203947.0 68 166915.5 69 135560.5 70 106057.5 71 81535.0 72 63407.0 73 50618.5 74 39721.0 75 27759.5 76 18273.5 77 13427.0 78 10454.5 79 6487.5 80 3081.5 81 1552.0 82 824.5 83 548.0 84 381.5 85 276.0 86 212.5 87 169.5 88 136.5 89 107.0 90 85.5 91 76.5 92 57.0 93 37.5 94 28.5 95 25.5 96 18.0 97 8.5 98 4.5 99 2.5 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.049085336801927205 2 0.004262633224326401 3 0.0295596266181123 4 0.008401352692131686 5 1.1462022478203261E-4 6 0.0016976184643392937 7 0.0018060430012952705 8 0.003144311571723327 9 0.008038904954307423 10-11 0.008650729127130434 12-13 0.0031598007912884665 14-15 3.237246889114164E-4 16-17 0.0013599534778192517 18-19 6.799767389096259E-4 20-21 0.001412616824340726 22-23 2.9894193760719316E-4 24-25 0.002524742789117745 26-27 0.004178991438674649 28-29 0.0056256845460586815 30-31 0.005901392654318166 32-33 0.00615386693322994 34-35 4.7087227478024207E-4 36-37 0.0022335454612931217 38-39 0.0014250081999928378 40-41 0.0017409882791216845 42-43 0.0025510744623784823 44-45 8.581027639087306E-4 46-47 7.620696026048654E-4 48-49 3.934261769545444E-4 50-51 9.85114364342875E-4 52-53 2.927462497811373E-4 54-55 0.0017735156402084774 56-57 8.162818710828538E-4 58-59 0.0027431407849862126 60-61 0.0010873432134727958 62-63 1.9051740065121635E-4 64-65 8.983747347780933E-5 66-67 1.5489219565139539E-6 68-69 7.125040999964189E-5 70-71 0.001795200547599673 72-73 3.516052841286676E-4 74-75 4.646765869541862E-6 76-77 0.0026796349847691405 78-79 0.0014265571219493518 80-81 1.084245369559768E-4 82-83 0.005501770789537565 84-85 2.9894193760719316E-4 86-87 2.122023080424117E-4 88-89 2.633167326073722E-5 90-91 0.001720852293687003 92-93 0.0045739665375857065 94-95 1.1462022478203261E-4 96-97 0.001787455937817103 98-99 0.0016062320689049702 100 8.42613544343591E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 3.2280516E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 25.295880305120306 #Duplication Level Percentage of deduplicated Percentage of total 1 72.4099035752174 18.31672253744002 2 14.334717032896375 7.252185725438319 3 4.9365789109439735 3.746253277440598 4 2.2560781939284986 2.2827793581042917 5 1.1551793543093447 1.4610639338777673 6 0.793832850717662 1.2048420464415848 7 0.5130638138487419 0.9084880576804409 8 0.35026109173794556 0.7088130121715067 9 0.29796890600528303 0.6783647202861555 >10 2.0868298785196235 11.217548737018477 >50 0.4533601408215774 8.008560019732299 >100 0.3137368789886024 18.68477986010621 >500 0.06622528051588698 11.0083476826684 >1k 0.03201632570590135 13.541563186486627 >5k 7.432975297947893E-5 0.15465572241593503 >10k+ 1.7343609028544932E-4 0.8250321226911775 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source ATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTT 38848 0.12034504033330817 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 9.293531739083724E-6 0.0 0.0 0.0 0.0 2 9.293531739083724E-6 0.0 0.0 0.0 0.0 3 9.293531739083724E-6 0.0 0.0 0.0 3.097843913027908E-6 4 1.548921956513954E-5 0.0 0.0 0.0 3.097843913027908E-6 5 2.4782751304223266E-5 3.097843913027908E-6 0.0 0.0 3.097843913027908E-6 6 2.4782751304223266E-5 3.097843913027908E-6 0.0 0.0 3.097843913027908E-6 7 2.4782751304223266E-5 3.097843913027908E-6 0.0 0.0 3.097843913027908E-6 8 2.7880595217251173E-5 3.097843913027908E-6 0.0 0.0 3.097843913027908E-6 9 4.0271970869362804E-5 3.097843913027908E-6 0.0 0.0 3.097843913027908E-6 10-11 1.0997345891249074E-4 3.097843913027908E-6 0.0 0.0 3.097843913027908E-6 12-13 3.748391134763769E-4 3.097843913027908E-6 0.0 0.0 3.097843913027908E-6 14-15 0.0011384576380377561 3.097843913027908E-6 0.0 0.0 3.097843913027908E-6 16-17 0.0025727593697696775 3.097843913027908E-6 0.0 0.0 3.097843913027908E-6 18-19 0.004563124083890108 3.097843913027908E-6 0.0 0.0 3.097843913027908E-6 20-21 0.007077024419312256 6.195687826055816E-6 0.0 0.0 3.097843913027908E-6 22-23 0.010190357551905303 6.195687826055816E-6 0.0 0.0 3.097843913027908E-6 24-25 0.01427021798536306 6.195687826055816E-6 0.0 0.0 3.097843913027908E-6 26-27 0.019087365270121455 6.195687826055816E-6 0.0 0.0 3.097843913027908E-6 28-29 0.02302937164944947 6.195687826055816E-6 0.0 0.0 3.097843913027908E-6 30-31 0.026376591997476127 6.195687826055816E-6 0.0 0.0 3.097843913027908E-6 32-33 0.029161553675288214 6.195687826055816E-6 0.0 0.0 4.646765869541862E-6 34-35 0.03186906925527461 6.195687826055816E-6 0.0 0.0 6.195687826055816E-6 36-37 0.034646286323304125 6.195687826055816E-6 0.0 0.0 6.195687826055816E-6 38-39 0.03709358301459617 6.195687826055816E-6 0.0 0.0 6.195687826055816E-6 40-41 0.03946808037393207 6.195687826055816E-6 0.0 0.0 6.195687826055816E-6 42-43 0.04223910175413553 6.195687826055816E-6 0.0 0.0 6.195687826055816E-6 44-45 0.0454995824725974 6.195687826055816E-6 0.0 0.0 6.195687826055816E-6 46-47 0.04953452416931625 9.293531739083724E-6 0.0 3.097843913027908E-6 6.195687826055816E-6 48-49 0.05408215903364122 1.2391375652111633E-5 0.0 3.097843913027908E-6 6.195687826055816E-6 50-51 0.0594631139105707 1.2391375652111633E-5 0.0 3.097843913027908E-6 6.195687826055816E-6 52-53 0.06643171379292698 1.2391375652111633E-5 0.0 3.097843913027908E-6 6.195687826055816E-6 54-55 0.07524507972549138 1.2391375652111633E-5 0.0 3.097843913027908E-6 6.195687826055816E-6 56-57 0.08671949357934675 1.2391375652111633E-5 0.0 6.195687826055816E-6 6.195687826055816E-6 58-59 0.10074343297362409 1.3940297608625588E-5 0.0 6.195687826055816E-6 6.195687826055816E-6 60-61 0.11819668557962333 1.858706347816745E-5 0.0 6.195687826055816E-6 9.293531739083724E-6 62-63 0.14004577869820917 1.858706347816745E-5 0.0 6.195687826055816E-6 1.0842453695597678E-5 64-65 0.1679743904961123 1.858706347816745E-5 0.0 9.293531739083724E-6 1.2391375652111633E-5 66-67 0.2029846734791972 1.858706347816745E-5 0.0 1.2391375652111633E-5 1.2391375652111633E-5 68-69 0.2461159542802847 1.858706347816745E-5 0.0 1.2391375652111633E-5 1.548921956513954E-5 70-71 0.2989713671243669 1.858706347816745E-5 0.0 1.2391375652111633E-5 1.548921956513954E-5 72-73 0.36218287216970135 1.858706347816745E-5 0.0 1.2391375652111633E-5 1.548921956513954E-5 74-75 0.43868567652388213 1.858706347816745E-5 0.0 1.2391375652111633E-5 1.548921956513954E-5 76-77 0.5306529176918982 1.858706347816745E-5 0.0 1.548921956513954E-5 1.858706347816745E-5 78-79 0.6376075277111432 1.858706347816745E-5 0.0 1.548921956513954E-5 1.858706347816745E-5 80-81 0.7634806705072497 1.858706347816745E-5 0.0 1.548921956513954E-5 1.858706347816745E-5 82-83 0.9097376262510797 1.858706347816745E-5 0.0 1.548921956513954E-5 1.858706347816745E-5 84-85 1.0783857977982756 1.858706347816745E-5 0.0 1.548921956513954E-5 1.858706347816745E-5 86-87 1.2705466046453533 1.858706347816745E-5 0.0 1.858706347816745E-5 1.858706347816745E-5 88 1.4312069856628067 1.858706347816745E-5 0.0 1.858706347816745E-5 1.858706347816745E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ATCTCGA 24460 0.0 51.814262 1 TCTCGAG 31640 0.0 40.13926 2 CTCGAGG 37790 0.0 33.445087 3 TCGAGGG 45755 0.0 27.614815 4 GATCCGG 41060 0.0 23.797192 1 TATGGAT 17605 0.0 22.354795 2 ATCCGGC 48325 0.0 20.140942 2 AGGGCGC 65240 0.0 19.372478 7 TATCTCG 13355 0.0 19.293808 2 CGAGGGC 70025 0.0 18.223217 5 GGGCGCG 69785 0.0 18.06363 8 GGATCCG 28635 0.0 17.389217 2 ATGGATC 28910 0.0 17.086351 1 TCCGGCG 58955 0.0 16.77253 3 GAGGGCG 80325 0.0 15.96839 6 TGGATCC 32075 0.0 15.913701 1 ATATCTC 18320 0.0 14.431749 1 GCGCCGT 69760 0.0 13.993887 9 CGGCGCG 70865 0.0 13.842006 5 CCGGCGC 75610 0.0 13.173559 4 >>END_MODULE