FastQCFastQC Report
Wed 25 May 2016
SRR491072_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR491072_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24893079
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTT284050.11410802175174875No Hit
GATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT270190.10854020910792113No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCTCGA194600.050.423941
TCTCGAG247050.039.8278922
CTCGAGG296400.032.990443
TCGAGGG344700.028.2783164
GATCCGG260650.025.2238981
TATCTCG100500.022.8274142
ATCCGGC321650.020.4473552
AGGGCGC499600.019.4640717
CGAGGGC512900.019.1422355
GGGCGCG535800.018.1578038
GGATCCG184800.017.5020712
GAGGGCG565350.017.4328676
TCCGGCG386700.017.0928363
ATATCTC143000.016.685741
GCGCCGT444150.014.7512479
CGGCGCG445450.014.7079035
TATGGAT178800.014.6976292
TGGATCC232750.014.3360691
ATGGATC200550.014.29118251
CCGGCGC501300.013.2661474
Produced by FastQC (version 0.11.5)