FastQCFastQC Report
Wed 25 May 2016
SRR491071_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR491071_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27620142
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA414190.149959402815525No Hit
ATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTT277700.10054256781156301No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCTCGA183050.050.2123761
TCTCGAG235000.038.9910622
CTCGAGG284350.032.2238433
TATCTCG82700.029.21992
GATCCGG308750.027.513671
TCGAGGG336400.027.0901134
ATCCGGC374800.022.666252
CGAGGGC485250.018.9060215
TCCGGCG455000.018.7225743
AGGGCGC506550.017.9349147
ATATCTC141800.017.8891961
GGATCCG212300.017.3172072
GGGCGCG542000.016.8138948
CGGCGCG531450.015.989025
TATGGAT188400.015.9456082
GCGCCGT538100.015.8003019
GAGGGCG582550.015.7401886
CCGGCGC573150.014.9816364
ATGGATC223550.014.8841151
TGGATCC264350.014.2069831
Produced by FastQC (version 0.11.5)