FastQCFastQC Report
Wed 25 May 2016
SRR491070_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR491070_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33066571
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTT363290.10986624527835076No Hit
GATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT348860.1055023213625628No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCTCGA242950.048.3308531
TCTCGAG289950.040.5845682
CTCGAGG363050.032.2314873
TCGAGGG408550.028.4719494
GATCCGG328700.023.9677091
ATCCGGC391950.020.0552832
CGAGGGC584300.020.0445085
AGGGCGC603050.019.3202637
TATCTCG122700.019.2345642
GGGCGCG639200.018.2349578
GAGGGCG670000.017.5227226
TCCGGCG481650.016.4373723
GGATCCG236650.016.2872641
TATGGAT204100.015.1723791
ATATCTC166000.014.6572751
CGGCGCG541750.014.5311225
GCGCCGT546150.014.3626399
ATGGATC254350.014.2472081
CCGGCGC575800.013.7698964
TGGATCC290250.013.3768071
Produced by FastQC (version 0.11.5)