FastQCFastQC Report
Wed 25 May 2016
SRR491069_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR491069_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35782052
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCTCGA226050.048.8617361
TCTCGAG290450.038.2002372
CTCGAGG340600.032.354733
TCGAGGG389100.028.2522074
GATCCGG334900.023.0315991
TATCTCG96400.022.9446331
ATCCGGC384150.020.1321662
AGGGCGC555150.019.8783457
GGGCGCG581700.019.0195358
CGAGGGC591950.018.7692035
GAGGGCG645550.017.1816736
GGATCCG241950.017.0217382
TCCGGCG504250.015.5236253
ATGGATC246250.014.5626041
CGGCGCG536700.014.4664465
TATGGAT220550.014.2324311
GCGCCGT544400.014.2103319
TGGATCC302500.013.9393631
GGCGCGC1091300.013.3508849
CCGGCGC610550.012.8474964
Produced by FastQC (version 0.11.5)