FastQCFastQC Report
Wed 25 May 2016
SRR491068_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR491068_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34687119
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCTCGA239900.046.2140351
TCTCGAG303450.036.4033162
CTCGAGG355500.030.888443
TCGAGGG406600.026.9482254
GATCCGG284800.022.653031
TATCTCG107500.022.563242
ATCCGGC325000.019.728372
AGGGCGC559100.019.5023127
CGAGGGC590950.018.706255
GGGCGCG603150.018.155898
GAGGGCG650900.016.9539226
TCCGGCG423300.015.3025463
GGATCCG202500.015.20474153
GCGCCGT448250.014.1337229
CGGCGCG452700.014.0471255
GGCGCGC1014000.013.7891039
ATATCTC182500.013.5886591
CGTCGAC422800.012.85567412-13
CCGGATC633650.012.27539714-15
ATGGATC255000.012.1795881
Produced by FastQC (version 0.11.5)