FastQCFastQC Report
Wed 25 May 2016
SRR491067_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR491067_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34817667
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTT351490.10095162320898755No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCTCGA238750.047.322581
TCTCGAG299400.037.679522
CTCGAGG367600.030.4716763
TCGAGGG413600.026.9231994
TATCTCG115250.022.5135562
GATCCGG308400.022.0329381
ATCCGGC365550.018.6066252
AGGGCGC604450.018.4121917
GGGCGCG636550.017.6314168
CGAGGGC642200.017.5135695
GAGGGCG678400.016.5436296
ATATCTC169600.015.9814531
GGATCCG221700.015.39283853
ATGGATC235850.014.8641481
TCCGGCG476750.014.45413
GCGCCGT487450.013.7973279
CGGCGCG495550.013.6482065
TATGGAT220850.013.4939092
GGCGCGC1089500.013.3425269
CGTCGAC464950.012.43828412-13
Produced by FastQC (version 0.11.5)