##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR491067_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 34817667 Sequences flagged as poor quality 0 Sequence length 100 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.495680396966286 33.0 31.0 34.0 30.0 34.0 2 31.782457538007932 34.0 31.0 34.0 30.0 34.0 3 31.86934187750144 34.0 31.0 34.0 30.0 34.0 4 35.29287269592187 37.0 35.0 37.0 33.0 37.0 5 35.02740758018049 37.0 35.0 37.0 32.0 37.0 6 34.99073576641422 37.0 35.0 37.0 32.0 37.0 7 34.95818832433546 37.0 35.0 37.0 32.0 37.0 8 34.85407040626817 37.0 35.0 37.0 32.0 37.0 9 36.47673935189282 39.0 37.0 39.0 32.0 39.0 10-11 36.51449288661414 39.0 37.0 39.0 32.0 39.0 12-13 36.50381623788866 39.0 37.0 39.0 32.0 39.0 14-15 37.829449902545164 40.0 38.0 41.0 32.0 41.0 16-17 37.744084073754856 40.0 38.0 41.0 32.0 41.0 18-19 37.73830381857578 40.0 38.0 41.0 32.0 41.0 20-21 37.69722023592218 40.0 38.0 41.0 32.0 41.0 22-23 37.600025297501986 40.0 38.0 41.0 32.0 41.0 24-25 37.530468784712085 40.0 38.0 41.0 32.0 41.0 26-27 37.35279458844845 40.0 38.0 41.0 31.5 41.0 28-29 37.16818993070386 40.0 37.0 41.0 31.0 41.0 30-31 37.006714177604145 40.0 37.0 41.0 31.0 41.0 32-33 36.868815765283756 40.0 37.0 41.0 30.0 41.0 34-35 36.74293360896352 40.0 36.5 41.0 30.0 41.0 36-37 36.603397105842845 40.0 36.0 41.0 30.0 41.0 38-39 36.451347529976665 40.0 36.0 41.0 30.0 41.0 40-41 36.20803231876506 40.0 35.5 41.0 29.5 41.0 42-43 35.93591504852982 40.0 35.0 41.0 28.0 41.0 44-45 35.66048947219812 39.5 35.0 41.0 27.0 41.0 46-47 35.340252694127955 39.0 35.0 41.0 26.0 41.0 48-49 35.06691637323087 39.0 35.0 41.0 24.5 41.0 50-51 34.80397582353809 39.0 35.0 41.0 24.0 41.0 52-53 34.64171542567743 39.0 34.0 40.5 23.0 41.0 54-55 34.538467683661864 38.5 34.0 41.0 23.0 41.0 56-57 34.30087204004795 38.0 34.0 40.5 23.0 41.0 58-59 34.01408746025402 38.0 34.0 40.0 22.0 41.0 60-61 33.69302671830367 37.0 33.5 40.0 21.5 41.0 62-63 33.36044296132765 36.5 33.0 40.0 20.0 41.0 64-65 33.006221554132274 36.0 33.0 39.0 19.5 41.0 66-67 32.73301427691867 36.0 33.0 39.0 18.5 41.0 68-69 32.603289516784685 35.0 33.0 39.0 18.5 41.0 70-71 32.30845271740924 35.0 33.0 38.5 18.0 40.0 72-73 31.966134031898232 35.0 33.0 37.0 16.0 39.5 74-75 31.63371776173286 35.0 33.0 37.0 12.0 39.0 76-77 30.923779198072058 35.0 31.5 36.0 9.0 39.0 78-79 30.998422611141635 35.0 32.0 36.0 7.0 37.5 80-81 30.82503893784727 35.0 32.0 35.5 4.5 37.0 82-83 30.569816754235717 35.0 32.0 35.0 2.0 37.0 84-85 30.348766518445935 35.0 32.0 35.0 2.0 36.0 86-87 30.12325835903939 35.0 32.0 35.0 2.0 36.0 88-89 29.93396554973083 35.0 31.5 35.0 2.0 36.0 90-91 29.718265686784815 35.0 31.0 35.0 2.0 35.5 92-93 29.50699703687786 35.0 31.0 35.0 2.0 35.0 94-95 29.263324621951263 35.0 31.0 35.0 2.0 35.0 96-97 29.009275018340546 35.0 31.0 35.0 2.0 35.0 98-99 28.718084772882687 34.0 30.0 35.0 2.0 35.0 100 28.042224081240136 34.0 29.0 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.3946994252090299 1101 2 0.4204501970010348 1101 3 0.45050132434516854 1101 4 0.38309167310680436 1101 5 0.45255599721191686 1101 6 0.4731971972891529 1101 7 0.46086794677317755 1101 8 0.5626633145514788 1101 9 0.6238616221536972 1101 10-11 0.5614639903112462 1101 12-13 0.6109670224263439 1101 14-15 0.6314734525294625 1101 16-17 0.6957971782532582 1101 18-19 0.6699775191031847 1101 20-21 0.7338855591920606 1101 22-23 0.7484554990897436 1101 24-25 0.7059323446297583 1101 26-27 0.7282557983059306 1101 28-29 0.6899570378571838 1101 30-31 0.6766873441322545 1101 32-33 0.7597402008668439 1101 34-35 0.7756437297755667 1101 36-37 0.7394531478286837 1101 38-39 0.7280354197412535 1101 40-41 0.7010055806127369 1101 42-43 0.7338317163333059 1101 44-45 0.6788599243701725 1101 46-47 0.6717639130411897 1101 48-49 0.693523394321808 1101 50-51 0.652987252074368 1101 52-53 0.6169865088276794 1101 54-55 0.6405393900532133 1101 56-57 0.6058572130822739 1101 58-59 0.63791269602131 1101 60-61 0.627011522128889 1101 62-63 0.5594742147758467 1101 64-65 0.5314750947485365 1101 66-67 0.5866402593747111 1101 68-69 0.7080994811600618 1101 70-71 0.7521541679622246 1101 72-73 0.6832380530944846 1101 74-75 0.6601399154552148 1101 76-77 0.6399699985913578 1101 78-79 0.6342256361926779 1101 80-81 0.6012907955550801 1101 82-83 0.5988615094226191 1101 84-85 0.5820530649675817 1101 86-87 0.5470225215479516 1101 88-89 0.5555153076443666 1101 90-91 0.5706881974432001 1101 92-93 0.5303785510579502 1101 94-95 0.48757760278034823 1101 96-97 0.49449268961021886 1101 98-99 0.4383642678336983 1101 100 0.4044324399626724 1102 1 0.23474289415838 1102 2 0.24342588442626223 1102 3 0.2676841963313379 1102 4 0.2519437496752346 1102 5 0.2854603177299424 1102 6 0.3211066030580412 1102 7 0.35458275119912486 1102 8 0.4273825579473609 1102 9 0.430948615966841 1102 10-11 0.41521452291136995 1102 12-13 0.3781264657687444 1102 14-15 0.38656549214059766 1102 16-17 0.4374373457375782 1102 18-19 0.40326281915328366 1102 20-21 0.5090372031012507 1102 22-23 0.4668226117312173 1102 24-25 0.4447670613836223 1102 26-27 0.4889506551048868 1102 28-29 0.4906670058213365 1102 30-31 0.43814621165470413 1102 32-33 0.45413054774104467 1102 34-35 0.4331743187872661 1102 36-37 0.38587397213262165 1102 38-39 0.37239433381152054 1102 40-41 0.42774185837811274 1102 42-43 0.39883934023139744 1102 44-45 0.407927105266765 1102 46-47 0.49805417713019295 1102 48-49 0.4629849006875233 1102 50-51 0.43179974270156407 1102 52-53 0.4162016050896824 1102 54-55 0.3359419773059429 1102 56-57 0.39412043900080107 1102 58-59 0.43441644736437723 1102 60-61 0.38295406480261107 1102 62-63 0.37184159096692326 1102 64-65 0.34456088286200526 1102 66-67 0.3434796320618929 1102 68-69 0.3963981054728123 1102 70-71 0.37374878987114357 1102 72-73 0.3938455206735796 1102 74-75 0.31463217092131757 1102 76-77 0.2511736787052392 1102 78-79 0.33563533001146695 1102 80-81 0.4031869246901074 1102 82-83 0.3590320852255644 1102 84-85 0.32025568599943455 1102 86-87 0.30506618836527366 1102 88-89 0.28764790272785845 1102 90-91 0.3086640623789414 1102 92-93 0.3020105725383111 1102 94-95 0.3543629947134015 1102 96-97 0.36477357516324105 1102 98-99 0.3640875564938284 1102 100 0.2783277635237269 1103 1 0.25010969108720005 1103 2 0.27647711712963385 1103 3 0.3020418158242393 1103 4 0.26306481728146736 1103 5 0.24227102141179557 1103 6 0.3232881084315764 1103 7 0.3377073069810308 1103 8 0.4255785438892161 1103 9 0.42182634526895413 1103 10-11 0.368022314325124 1103 12-13 0.3653303306828235 1103 14-15 0.4074900118019329 1103 16-17 0.4502024672157603 1103 18-19 0.42687349395967544 1103 20-21 0.44706690061887144 1103 22-23 0.3254337020855971 1103 24-25 0.41981311040349567 1103 26-27 0.447045906038511 1103 28-29 0.5091399920361965 1103 30-31 0.5315183472264167 1103 32-33 0.40914142693861777 1103 34-35 0.48091852287370074 1103 36-37 0.40676460412716864 1103 38-39 0.4682567134472393 1103 40-41 0.45589144032967255 1103 42-43 0.399766018175157 1103 44-45 0.4528438935780912 1103 46-47 0.3391620047827786 1103 48-49 0.4437729426828483 1103 50-51 0.4014115658361064 1103 52-53 0.42170800399016883 1103 54-55 0.37243216452977634 1103 56-57 0.3732478768070351 1103 58-59 0.43363801336361973 1103 60-61 0.3616972893761954 1103 62-63 0.40900607259319344 1103 64-65 0.4364075151974163 1103 66-67 0.35754754222650575 1103 68-69 0.45643235351126066 1103 70-71 0.4802804575599353 1103 72-73 0.44223149926372685 1103 74-75 0.45750390762831117 1103 76-77 0.4305981399676071 1103 78-79 0.4512538722415904 1103 80-81 0.42674941525806886 1103 82-83 0.468068631644158 1103 84-85 0.4593575060772821 1103 86-87 0.5070225655536049 1103 88-89 0.553291089047601 1103 90-91 0.5405883370635145 1103 92-93 0.5254419455338848 1103 94-95 0.5387416632969781 1103 96-97 0.6029470414936426 1103 98-99 0.6585162867395198 1103 100 0.745147095078881 1104 1 -0.08002703246913256 1104 2 -0.03822735947684919 1104 3 -0.0649390492467461 1104 4 -0.050965791072016486 1104 5 -0.05635137225430498 1104 6 0.009259630379617079 1104 7 -0.03242527859133304 1104 8 0.025829301211921063 1104 9 -0.14050428090980915 1104 10-11 -0.03234119356149279 1104 12-13 -0.1661990540833287 1104 14-15 -0.16310685618842768 1104 16-17 -0.06929757436971329 1104 18-19 -0.12305598724083922 1104 20-21 -0.21500943584051413 1104 22-23 -0.13858239561671581 1104 24-25 -0.14043835796151427 1104 26-27 -0.19375933095004427 1104 28-29 -0.17976940607228897 1104 30-31 -0.0707179524039887 1104 32-33 -0.0739948743324419 1104 34-35 -0.08625710678894905 1104 36-37 -0.11333150303892836 1104 38-39 -0.14226808623503473 1104 40-41 -0.20385990603650583 1104 42-43 -0.05649099454891626 1104 44-45 -0.15800833579960738 1104 46-47 -0.2240147686821743 1104 48-49 -0.1565790863491543 1104 50-51 -0.107640251050654 1104 52-53 -0.0769489853901888 1104 54-55 -0.10266167556939365 1104 56-57 -0.07331319134447511 1104 58-59 -0.1428936464200703 1104 60-61 -0.1308747356650386 1104 62-63 -0.08769433185435105 1104 64-65 -0.1017296310813478 1104 66-67 0.02564603219985173 1104 68-69 0.02042803133778648 1104 70-71 -0.11784128727022392 1104 72-73 -0.05541558004864555 1104 74-75 -0.1235562135499002 1104 76-77 -0.14967428205612876 1104 78-79 -0.1528931951680299 1104 80-81 -0.1359785006330121 1104 82-83 -0.11099940917794981 1104 84-85 -0.11090326905288705 1104 86-87 -0.10042176811134595 1104 88-89 -0.03853675665937928 1104 90-91 -0.04944484944153871 1104 92-93 -0.11857012302249004 1104 94-95 -0.049451132119905594 1104 96-97 -0.05094868256446006 1104 98-99 -0.004473092829574199 1104 100 0.0035291949084452767 1105 1 -0.46918185347019303 1105 2 -0.4006899479233894 1105 3 -0.4138777532843356 1105 4 -0.344473434962012 1105 5 -0.3978521543649123 1105 6 -0.6505942608022863 1105 7 -0.43362112372356876 1105 8 -0.41106062822337464 1105 9 -0.48262484380128967 1105 10-11 -0.4933425838161938 1105 12-13 -0.5067729655256343 1105 14-15 -0.632238472976077 1105 16-17 -0.5894544584728152 1105 18-19 -0.8159975243624444 1105 20-21 -0.5991039068328661 1105 22-23 -0.5741099442361772 1105 24-25 -0.7155684216832512 1105 26-27 -0.9665578143403337 1105 28-29 -0.8485113964200295 1105 30-31 -0.7784215984986886 1105 32-33 -0.9686874037186044 1105 34-35 -0.8930348811729516 1105 36-37 -0.7313765264533885 1105 38-39 -0.6464662199657383 1105 40-41 -0.7689629883977815 1105 42-43 -0.6471961027503212 1105 44-45 -0.7582785105771137 1105 46-47 -0.6471380174092545 1105 48-49 -0.579818191210471 1105 50-51 -0.5782792718368555 1105 52-53 -0.5609655538529879 1105 54-55 -0.6210233670790828 1105 56-57 -0.6401074881828563 1105 58-59 -0.5918793892696854 1105 60-61 -0.5217950893402943 1105 62-63 -0.5742918091255689 1105 64-65 -0.5722372931043154 1105 66-67 -0.6254498590620798 1105 68-69 -0.6085349208909179 1105 70-71 -0.6538737302071347 1105 72-73 -0.6417450372716473 1105 74-75 -0.6341583270520594 1105 76-77 -0.7355736969568767 1105 78-79 -0.6459810958805754 1105 80-81 -0.5800520426508164 1105 82-83 -0.5020540234183848 1105 84-85 -0.47306039738483463 1105 86-87 -0.4689909354576116 1105 88-89 -0.49089988891086733 1105 90-91 -0.5315297294963237 1105 92-93 -0.5039220569420344 1105 94-95 -0.6796313646868377 1105 96-97 -0.6697081007028203 1105 98-99 -0.47580176480143166 1105 100 -0.5035182768227671 1106 1 -0.6320569511106271 1106 2 -0.6550734558045477 1106 3 -0.5509403503646801 1106 4 -0.46229459774057347 1106 5 -0.5349099382360052 1106 6 -0.5655751549805927 1106 7 -0.6252435346614575 1106 8 -0.5384888129462553 1106 9 -0.5878725743392508 1106 10-11 -0.695188590000761 1106 12-13 -0.7111817739882653 1106 14-15 -0.8234245670628937 1106 16-17 -0.8850830073782632 1106 18-19 -0.8634387670459347 1106 20-21 -0.792836863381666 1106 22-23 -0.9609750514519178 1106 24-25 -0.9412091150985091 1106 26-27 -0.8934880348411411 1106 28-29 -0.7932940142505487 1106 30-31 -0.9146735475466627 1106 32-33 -0.9147719461153159 1106 34-35 -0.9461848910082296 1106 36-37 -0.9547280292482228 1106 38-39 -1.0160972168671378 1106 40-41 -0.9361501564328663 1106 42-43 -1.0592270149551268 1106 44-45 -1.0294697344381234 1106 46-47 -0.9976041814839576 1106 48-49 -1.0669915183568222 1106 50-51 -1.0638944049048433 1106 52-53 -1.0237857195137323 1106 54-55 -0.9513406518471967 1106 56-57 -0.9224170887834262 1106 58-59 -0.9073072268010876 1106 60-61 -0.9770869633292776 1106 62-63 -0.9367460081634178 1106 64-65 -0.9084619794127491 1106 66-67 -0.8917282941050289 1106 68-69 -0.955128396722813 1106 70-71 -0.8596358929062475 1106 72-73 -0.9339193972125628 1106 74-75 -0.8666438362682065 1106 76-77 -0.9321046739663537 1106 78-79 -0.9066782414863148 1106 80-81 -0.9751603509243694 1106 82-83 -0.9230420728356563 1106 84-85 -0.8481478751464593 1106 86-87 -0.8809704476176847 1106 88-89 -0.8326246458475985 1106 90-91 -0.824944180243488 1106 92-93 -0.8510544042004504 1106 94-95 -0.8220739104887436 1106 96-97 -0.8363006358473086 1106 98-99 -0.7857413408024883 1106 100 -0.8162162629550274 1107 1 -0.8156957301990211 1107 2 -0.7354023362847144 1107 3 -0.728854296187027 1107 4 -0.6579468767321544 1107 5 -0.7700770386924063 1107 6 -0.7648552532799542 1107 7 -0.8144992270902307 1107 8 -0.9039670874295496 1107 9 -0.9284113944373686 1107 10-11 -0.9536093525324318 1107 12-13 -0.9538546633320308 1107 14-15 -1.1081760567941714 1107 16-17 -1.1712131878984167 1107 18-19 -1.1237641688213387 1107 20-21 -1.1698019976094969 1107 22-23 -1.247568207114675 1107 24-25 -1.2162604671218702 1107 26-27 -1.2239430843385577 1107 28-29 -1.2130086545760932 1107 30-31 -1.2286904890985895 1107 32-33 -1.279826065253161 1107 34-35 -1.2611811354889326 1107 36-37 -1.290093454900834 1107 38-39 -1.3203356632247178 1107 40-41 -1.3354064365105671 1107 42-43 -1.2777887294612427 1107 44-45 -1.3021917489845976 1107 46-47 -1.2396513116765746 1107 48-49 -1.3370237304539287 1107 50-51 -1.2842020371588774 1107 52-53 -1.281192817566584 1107 54-55 -1.1952152223178487 1107 56-57 -1.1863913839695996 1107 58-59 -1.1932638025545614 1107 60-61 -1.2205964268465053 1107 62-63 -1.207601005667982 1107 64-65 -1.169897933665311 1107 66-67 -1.2022297043178085 1107 68-69 -1.221174186403445 1107 70-71 -1.2462175189807638 1107 72-73 -1.2348320880680674 1107 74-75 -1.2077605748885283 1107 76-77 -1.2804095481446147 1107 78-79 -1.2603671642613712 1107 80-81 -1.2062439634674327 1107 82-83 -1.1917023710952037 1107 84-85 -1.2450014726192578 1107 86-87 -1.2783908414812117 1107 88-89 -1.3046578030581806 1107 90-91 -1.3025385266543843 1107 92-93 -1.2915318612760807 1107 94-95 -1.4636220188752311 1107 96-97 -1.3808683489750209 1107 98-99 -1.3040782001956899 1107 100 -1.2947872003502496 1108 1 -1.6130507093892135 1108 2 -1.5125948422983484 1108 3 -1.4424401344664801 1108 4 -1.3853340923122062 1108 5 -1.487328954585287 1108 6 -1.5221800773109138 1108 7 -1.579250119442726 1108 8 -2.338138942400647 1108 9 -3.167172990580845 1108 10-11 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0.5312793086899745 2305 80-81 0.4990975032781577 2305 82-83 0.48110178028446526 2305 84-85 0.5119990881360224 2305 86-87 0.5197863169039891 2305 88-89 0.523753592977382 2305 90-91 0.527742161209968 2305 92-93 0.5730763451642069 2305 94-95 0.5401667181386856 2305 96-97 0.6817080104849538 2305 98-99 0.6825792342657486 2305 100 0.7355577470855046 2306 1 0.07987321893237365 2306 2 0.013079847319840354 2306 3 -0.009045593399687135 2306 4 0.03505534691486645 2306 5 0.06382594071788361 2306 6 0.03680075335815047 2306 7 0.04307166675049956 2306 8 0.0832981948249909 2306 9 0.10862356955857422 2306 10-11 0.059334301715225024 2306 12-13 0.02929420183048137 2306 14-15 0.02813044463218972 2306 16-17 0.055697100980125924 2306 18-19 0.07862906670374059 2306 20-21 0.0270228824339398 2306 22-23 0.010018244967334056 2306 24-25 0.03181194436985635 2306 26-27 0.06769632743593945 2306 28-29 0.08805473543434772 2306 30-31 0.12958104046716556 2306 32-33 0.1365980636633708 2306 34-35 0.0979732805651281 2306 36-37 0.0600726129097211 2306 38-39 0.0842958492758683 2306 40-41 0.09975731509219798 2306 42-43 0.16561957061937704 2306 44-45 0.08097402096286999 2306 46-47 0.10729770086320656 2306 48-49 0.12366018380269139 2306 50-51 0.10428939063127984 2306 52-53 0.1195095551361618 2306 54-55 0.1165556955328313 2306 56-57 0.11063176218227255 2306 58-59 0.14742511911416756 2306 60-61 0.13694924570106792 2306 62-63 0.11659685712029244 2306 64-65 0.1863465272901763 2306 66-67 0.16729135494158953 2306 68-69 0.1573132128503616 2306 70-71 0.15384510976217314 2306 72-73 0.07517848708795327 2306 74-75 0.10951165456440037 2306 76-77 0.1457244804867024 2306 78-79 0.1313984368496186 2306 80-81 0.0694543102291405 2306 82-83 0.08578922395306421 2306 84-85 0.07318055002129142 2306 86-87 0.0717571194519877 2306 88-89 0.10842400767311844 2306 90-91 0.08592302398232121 2306 92-93 0.15509724912969958 2306 94-95 0.20743568048473193 2306 96-97 0.2659052486629747 2306 98-99 0.3117996369277236 2306 100 0.2583621151643207 2307 1 -0.6467038806236971 2307 2 -0.8012657977240281 2307 3 -0.8368243269315272 2307 4 -0.5627906577593862 2307 5 -0.6448333206720349 2307 6 -0.5116304306849102 2307 7 -0.4542861336037376 2307 8 -0.3576748390199995 2307 9 -0.3264587653694164 2307 10-11 -0.3905661829171194 2307 12-13 -0.36175371710880455 2307 14-15 -0.4497942754342077 2307 16-17 -0.4345705480873647 2307 18-19 -0.4562462634712361 2307 20-21 -0.5014572736784402 2307 22-23 -0.47291829651963013 2307 24-25 -0.4591300649249206 2307 26-27 -0.5355777665401007 2307 28-29 -0.5616750827662926 2307 30-31 -0.4656303971638849 2307 32-33 -0.4541397326987209 2307 34-35 -0.5190609706819913 2307 36-37 -0.5666853965175065 2307 38-39 -0.5213557337094628 2307 40-41 -0.565262675749949 2307 42-43 -0.5390088545544316 2307 44-45 -0.5464434294401812 2307 46-47 -0.5130365079067118 2307 48-49 -0.4808404349153008 2307 50-51 -0.41742419239926676 2307 52-53 -0.41823825094370903 2307 54-55 -0.46424120299537464 2307 56-57 -0.4789970546855713 2307 58-59 -0.5104185492939521 2307 60-61 -0.4500462378679444 2307 62-63 -0.41047530036319557 2307 64-65 -0.462809663353859 2307 66-67 -0.4575318645594635 2307 68-69 -0.5007821098689504 2307 70-71 -0.581778839977865 2307 72-73 -0.5538901551621365 2307 74-75 -0.5316453705797954 2307 76-77 -0.49335606637125196 2307 78-79 -0.43328407585779516 2307 80-81 -0.4499181718656651 2307 82-83 -0.4928386307791186 2307 84-85 -0.5081339382076138 2307 86-87 -0.5376227285955935 2307 88-89 -0.49513334463670944 2307 90-91 -0.541016824766789 2307 92-93 -0.5311659247480875 2307 94-95 -0.46062696099452793 2307 96-97 -0.4938870858382174 2307 98-99 -0.5433276586427347 2307 100 -0.6166683427797324 2308 1 -1.1508778370709045 2308 2 -1.0806020383557602 2308 3 -1.0449838000310017 2308 4 -0.8920589699132719 2308 5 -1.310251570194147 2308 6 -1.3411084188610047 2308 7 -1.372978469607041 2308 8 -1.5092220864033123 2308 9 -1.5842874995019258 2308 10-11 -1.450656501067094 2308 12-13 -1.222830229615461 2308 14-15 -1.4101081677998266 2308 16-17 -1.3533360947852202 2308 18-19 -1.4252075087561735 2308 20-21 -1.3827911570065154 2308 22-23 -1.346501413031639 2308 24-25 -1.3398072892477373 2308 26-27 -1.3956759570513242 2308 28-29 -1.3827341122397954 2308 30-31 -1.337441537910827 2308 32-33 -1.3932651740280164 2308 34-35 -1.4008038161318979 2308 36-37 -1.440804512027583 2308 38-39 -1.4277674988780902 2308 40-41 -1.36984876515276 2308 42-43 -1.4075734946226603 2308 44-45 -1.4852044855084472 2308 46-47 -1.5029893199501672 2308 48-49 -1.419361117650297 2308 50-51 -1.3501724425967865 2308 52-53 -1.4160622541713437 2308 54-55 -1.3720121891313468 2308 56-57 -1.4663539227747364 2308 58-59 -1.4660539040542133 2308 60-61 -1.4956328185182386 2308 62-63 -1.5203203448293579 2308 64-65 -1.4906815015951018 2308 66-67 -1.4854071847676735 2308 68-69 -1.501425307158172 2308 70-71 -1.507084066024003 2308 72-73 -1.424236678856623 2308 74-75 -1.3825498175422304 2308 76-77 -1.392972127448676 2308 78-79 -1.4551018776902573 2308 80-81 -1.507563409533308 2308 82-83 -1.4802357629855045 2308 84-85 -1.4544905345548713 2308 86-87 -1.5693760436575879 2308 88-89 -1.53067567112274 2308 90-91 -1.508666202467925 2308 92-93 -1.5334883200468425 2308 94-95 -1.6116222601067385 2308 96-97 -2.1008960005735204 2308 98-99 -1.9346744263186224 2308 100 -1.8310727709897918 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 478728.0 3 155501.0 4 89220.0 5 75004.0 6 72459.0 7 77244.0 8 83844.0 9 90227.0 10 97927.0 11 99909.0 12 101960.0 13 119587.0 14 159863.0 15 225505.0 16 285705.0 17 277067.0 18 224699.0 19 160832.0 20 135711.0 21 133290.0 22 143061.0 23 164925.0 24 196573.0 25 235281.0 26 283841.0 27 339423.0 28 408327.0 29 487023.0 30 590542.0 31 726066.0 32 912103.0 33 1191354.0 34 1665846.0 35 2520104.0 36 4398045.0 37 7665976.0 38 7894440.0 39 1846012.0 40 4443.0 >>END_MODULE >>Per base sequence content pass #Base G A T C 1 21.589984617478347 27.295730725561203 27.487148907680513 23.627135749279933 2 24.496925655587436 26.79582465993485 26.379165496642837 22.32808418783487 3 24.247654179847714 25.665721554570098 26.117414251886018 23.96921001369617 4 24.829436346193912 25.24714354658821 26.18539121813232 23.738028889085562 5 25.191960041475063 25.583210066607116 25.87248428169726 23.35234561022056 6 25.174598860974807 25.45974720247626 25.222468237173963 24.143185699374975 7 25.14499927866914 25.85806994608788 25.47567124625839 23.52125952898459 8 24.734183914935176 25.676840914862627 25.717881072178344 23.87109409802385 9 24.35719296782333 25.381177827726248 25.915692163077725 24.345937041372697 10-11 24.39821562163426 25.588763377114937 25.801946190909792 24.21107481034101 12-13 24.115653888973885 25.666080027244863 25.95708471758853 24.261181366192723 14-15 23.892746610181515 25.892231566523726 26.294821249233053 23.92020057406171 16-17 23.74813421939252 25.791876731910484 26.565438702738902 23.894550345958095 18-19 23.389336067356886 25.753649684199253 27.14269933624141 23.714314912202447 20-21 23.08844854203065 25.511117082785752 27.793580838790476 23.60685353639312 22-23 23.135306872503282 25.47352191473962 28.130170593623266 23.26100061913383 24-25 23.05029717162606 25.345035218308936 28.49745603578181 23.1072115742832 26-27 23.10312154596833 25.410986819167782 28.51428960497198 22.971602029891905 28-29 23.14222843043985 25.447621639266167 28.472922797349792 22.937227132944198 30-31 23.131543182371942 25.388553614124287 28.437854069553577 23.042049133950197 32-33 23.167990492614564 25.45094579108884 28.32689340821219 23.054170308084405 34-35 23.230996552997617 25.492976345778224 28.344164262951644 22.93186283827252 36-37 23.231013765189793 25.462483977913188 28.256082763331612 23.050419493565407 38-39 23.26111702170973 25.498563234093442 28.17188547687434 23.068434267322488 40-41 23.29955383173996 25.61887941129239 28.035870449467627 23.045696307500023 42-43 23.411020563803948 25.635203446514083 27.706248105034213 23.247527884647752 44-45 23.447127599748356 25.7940954798893 27.397624789622764 23.361152130739583 46-47 23.683509780834008 25.937432188740306 27.00893877164737 23.37011925877832 48-49 23.762535003572843 25.924179483107622 26.69228131026275 23.621004203056785 50-51 23.75421933570495 26.027911052081375 26.523604864225938 23.694264747987738 52-53 23.84307316445923 26.093070500547444 26.386617782425414 23.67723855256791 54-55 23.869234511820487 26.049471645309602 26.31077112901781 23.770522713852106 56-57 23.850723926582297 26.103245362237292 26.294238312323003 23.751792398857408 58-59 23.93237934864308 26.155444378928166 26.256474120606693 23.65570215182206 60-61 23.86900688876986 26.13079852497588 26.216305900161107 23.783888686093153 62-63 23.886125922169732 26.168979195590364 26.220328304867778 23.72456657737213 64-65 23.931041308501285 26.243577867003708 26.20033953058409 23.625041293910915 66-67 23.919586902566124 26.247064855223506 26.124202688023857 23.709145554186513 68-69 23.87940205633732 26.34568221105411 26.099067694621354 23.675848037987215 70-71 23.92622427640979 26.439702058042315 26.062848627012976 23.571225038534926 72-73 23.917658841301716 26.45469180745565 25.94650453655442 23.681144814688217 74-75 23.89177647579899 26.586642492709416 25.924855855340795 23.596725176150795 76-77 23.90052215421644 26.675873775393267 25.814672590924626 23.608931479465667 78-79 23.885369097230022 26.710782171718794 25.756042593570292 23.647806137480888 80-81 23.890337140225824 26.770831141808532 25.69814063676131 23.64069108120433 82-83 23.88806212823415 26.898478393399966 25.633284715709255 23.580174762656632 84-85 23.845758092641507 26.90855224444852 25.574415178906413 23.67127448400356 86-87 23.837951181558097 27.03047615105822 25.519019915067805 23.612552752315878 88-89 23.97124205863429 27.1164376153124 25.360110443711235 23.552209882342073 90-91 23.90863126552425 27.11167786654406 25.301724889399253 23.677965978532438 92-93 23.921920083140762 27.226866835377844 25.263222336762052 23.587990744719338 94-95 24.002287460274722 27.296018289112585 25.17298735226215 23.52870689835055 96-97 23.94202424522745 27.346789711442987 25.022013517210034 23.689172526119528 98-99 23.929314422233055 27.425192969968936 24.974307745183555 23.671184862614453 100 24.03170135661246 27.560371787708952 24.651328390149615 23.756598465528974 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 4339.0 1 2804.0 2 1083.5 3 888.5 4 943.5 5 1054.0 6 1223.0 7 1470.0 8 1755.5 9 2090.5 10 2568.0 11 3344.5 12 4204.5 13 6500.5 14 13622.5 15 19031.5 16 26645.5 17 27973.0 18 23071.0 19 26509.0 20 33849.5 21 41909.0 22 50072.5 23 61620.0 24 75790.0 25 91932.0 26 109039.5 27 131246.0 28 161461.5 29 194982.5 30 230517.0 31 273980.5 32 326805.5 33 384462.5 34 450449.5 35 527337.0 36 620713.5 37 742429.0 38 856780.0 39 928926.5 40 980824.0 41 1066540.0 42 1225778.0 43 1366010.0 44 1474046.5 45 1566517.5 46 1576657.0 47 1522330.0 48 1466023.5 49 1460419.5 50 1437488.0 51 1375078.0 52 1278699.5 53 1146891.0 54 1061788.5 55 1004859.5 56 918978.0 57 828773.5 58 764243.0 59 704976.5 60 631856.5 61 563242.0 62 502845.0 63 441870.5 64 385641.5 65 328984.0 66 272670.0 67 226325.0 68 188021.0 69 152440.5 70 118046.5 71 88135.0 72 65932.5 73 49823.5 74 36988.5 75 25963.0 76 17246.5 77 11560.0 78 7798.0 79 4773.0 80 2568.5 81 1433.5 82 876.0 83 591.5 84 452.0 85 343.5 86 243.0 87 190.0 88 147.0 89 105.0 90 84.5 91 73.0 92 59.0 93 49.0 94 36.0 95 28.0 96 23.0 97 14.5 98 6.0 99 2.0 100 1.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.11455104099881248 2 0.0 3 0.0018180425471930673 4 0.014372014069753726 5 0.004500588738470042 6 0.0 7 2.297684103877494E-4 8 0.0015767857162859303 9 0.001301063623820631 10-11 0.0024010798885519815 12-13 0.004484792160255884 14-15 0.0034493982609460883 16-17 0.002313480682091652 18-19 0.0031392109069226268 20-21 0.0041688605959727286 22-23 0.00600987998420457 24-25 0.008917886428174525 26-27 0.005518750007000756 28-29 0.007242013084908877 30-31 0.0055833723724223105 32-33 0.005129579761906505 34-35 0.0075665609645815726 36-37 0.005903612094400237 38-39 0.006170717871475995 40-41 0.0053938134338524174 42-43 0.004983102400284316 44-45 0.0038500569265597262 46-47 0.003911807186851434 48-49 0.0024814988321876937 50-51 0.0037782542983135544 52-53 0.0012867030981713967 54-55 4.2219945408748956E-4 56-57 4.351239271718004E-4 58-59 5.65804710579833E-4 60-61 5.816012887939907E-4 62-63 3.4608866814654757E-4 64-65 5.313394490216705E-4 66-67 0.0017304433407327377 68-69 0.001072731265997805 70-71 0.0015796578214157773 72-73 0.005059213186225257 74-75 0.004846677406616589 76-77 0.0010540625826538006 78-79 0.00166869308044103 80-81 0.002224445423066399 82-83 0.0011172488955104314 84-85 0.0026696217181926634 86-87 0.004427350057658946 88-89 8.415268030451322E-4 90-91 0.0022057767397223945 92-93 0.001638535976577638 94-95 0.002548993302739095 96-97 0.001214900469925225 98-99 7.68288122234037E-4 100 0.0035585382558802688 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 3.4817667E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 35.323991407615225 #Duplication Level Percentage of deduplicated Percentage of total 1 70.80012877529497 25.009431405165707 2 15.315179636828411 10.819865477948207 3 5.476943475942828 5.80402512852596 4 2.635825967344043 3.7243157528972026 5 1.3968598380702906 2.4671332458818864 6 0.8550514551590637 1.8122298153064593 7 0.5625479025278243 1.3910006092685379 8 0.4118485175752789 1.1638506796854573 9 0.2866225356906445 0.9112186789168691 >10 1.8254837729891424 12.874748243415398 >50 0.1948167346224178 4.757383640851845 >100 0.1870469757423892 14.460647441198308 >500 0.04436146299892218 10.782120070767888 >1k 0.007127443858755364 3.1107150697710915 >5k 3.273796951647313E-5 0.08977988975401896 >10k+ 1.2276738568425238E-4 0.8215348506449628 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source ATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTT 35149 0.10095162320898755 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 2.8721051298468676E-6 0.0 0.0 0.0 2 0.0 1.148842051938747E-5 0.0 0.0 2.8721051298468676E-6 3 0.0 1.4360525649234338E-5 0.0 0.0 5.744210259693735E-6 4 5.744210259693735E-6 2.297684103877494E-5 0.0 0.0 5.744210259693735E-6 5 5.744210259693735E-6 3.1593156428315546E-5 0.0 0.0 5.744210259693735E-6 6 5.744210259693735E-6 3.1593156428315546E-5 0.0 0.0 5.744210259693735E-6 7 5.744210259693735E-6 3.733736668800928E-5 0.0 0.0 5.744210259693735E-6 8 5.744210259693735E-6 4.595368207754988E-5 0.0 0.0 5.744210259693735E-6 9 1.148842051938747E-5 4.595368207754988E-5 0.0 0.0 5.744210259693735E-6 10-11 3.590131412308585E-5 4.882578720739675E-5 0.0 0.0 7.18026282461717E-6 12-13 1.4791341418711368E-4 5.4569997467090484E-5 0.0 1.4360525649234338E-6 8.616315389540603E-6 14-15 3.489607732763944E-4 6.605841798647796E-5 0.0 2.8721051298468676E-6 8.616315389540603E-6 16-17 7.395670709355684E-4 8.329104876555916E-5 0.0 2.8721051298468676E-6 1.5796578214157773E-5 18-19 0.0012823949404766263 9.477946928494663E-5 0.0 4.308157694770302E-6 2.1540788473851507E-5 20-21 0.001945851225471253 1.019597321095638E-4 0.0 7.18026282461717E-6 2.297684103877494E-5 22-23 0.002885029602931179 1.0913999493418097E-4 0.0 8.616315389540603E-6 2.297684103877494E-5 24-25 0.0040568484959087004 1.2062841545356845E-4 0.0 8.616315389540603E-6 2.8721051298468676E-5 26-27 0.005738466049434041 1.278086782781856E-4 0.0 8.616315389540603E-6 2.8721051298468676E-5 28-29 0.007520607282504023 1.3642499366772623E-4 0.0 8.616315389540603E-6 2.8721051298468676E-5 30-31 0.008709658806260626 1.4934946675203712E-4 0.0 1.148842051938747E-5 2.8721051298468676E-5 32-33 0.009396091932294027 1.5652972957665428E-4 0.0 1.148842051938747E-5 2.8721051298468676E-5 34-35 0.010139967160924367 1.6658209753111833E-4 0.0 1.2924473084310904E-5 2.8721051298468676E-5 36-37 0.01106191290760521 1.780705180505058E-4 0.0 1.4360525649234338E-5 2.8721051298468676E-5 38-39 0.01198960286454575 1.7950657061542921E-4 0.0 1.5796578214157773E-5 2.8721051298468676E-5 40-41 0.012958938345869067 1.8381472831019953E-4 0.0 2.0104735908928074E-5 2.8721051298468676E-5 42-43 0.014168094605534597 1.8525078087512295E-4 0.0 2.0104735908928074E-5 2.8721051298468676E-5 44-45 0.015822427160326395 1.9817525395943386E-4 0.0 2.0104735908928074E-5 2.8721051298468676E-5 46-47 0.018226379154008224 2.0535551678405105E-4 0.0 2.1540788473851507E-5 2.8721051298468676E-5 48-49 0.021068327179991696 2.1109972704374478E-4 0.0 2.297684103877494E-5 3.0157103863392113E-5 50-51 0.024520597546067634 2.1684393730343852E-4 0.0 2.4412893603698375E-5 3.446526155816241E-5 52-53 0.029348606269340216 2.254602526929791E-4 0.0 2.7284998733545242E-5 3.446526155816241E-5 54-55 0.0353656665163694 2.3694867321236656E-4 0.0 3.1593156428315546E-5 3.733736668800928E-5 56-57 0.043266827728578136 2.4700104116683063E-4 0.0 3.1593156428315546E-5 3.733736668800928E-5 58-59 0.053413975152327126 2.498731462966775E-4 0.0 3.733736668800928E-5 3.8773419252932714E-5 60-61 0.06662996690731748 2.5274525142652437E-4 2.8721051298468676E-6 4.595368207754988E-5 4.020947181785615E-5 62-63 0.08351076480799245 2.627976193809884E-4 2.8721051298468676E-6 4.595368207754988E-5 4.595368207754988E-5 64-65 0.10491943644587101 2.7284998733545247E-4 2.8721051298468676E-6 4.595368207754988E-5 4.7389734642473316E-5 66-67 0.13246005253597262 2.8146630272499304E-4 5.744210259693735E-6 4.7389734642473316E-5 4.882578720739675E-5 68-69 0.16648013779900878 2.900826181145336E-4 5.744210259693735E-6 4.882578720739675E-5 5.026183977232018E-5 70-71 0.20872593215392632 2.943907758093039E-4 8.616315389540603E-6 4.882578720739675E-5 5.169789233724362E-5 72-73 0.25973595531257165 3.001349860689977E-4 8.616315389540603E-6 4.882578720739675E-5 5.169789233724362E-5 74-75 0.3223248703021946 3.073152488936149E-4 8.616315389540603E-6 4.882578720739675E-5 5.169789233724362E-5 76-77 0.3987616401753742 3.1880366941300234E-4 8.616315389540603E-6 5.4569997467090484E-5 5.169789233724362E-5 78-79 0.49068910906638286 3.3316419506223665E-4 8.616315389540603E-6 5.600605003201392E-5 5.169789233724362E-5 80-81 0.5983111964394399 3.446526155816241E-4 8.616315389540603E-6 6.0314207726784225E-5 5.600605003201392E-5 82-83 0.7242300295421862 3.6332129892562877E-4 8.616315389540603E-6 6.749447055140139E-5 5.744210259693735E-5 84-85 0.8725125092384851 3.8055392970471E-4 8.616315389540603E-6 7.18026282461717E-5 6.175026029170766E-5 86-87 1.042855628437138 3.8773419252932716E-4 8.616315389540603E-6 7.467473337601856E-5 6.318631285663109E-5 88 1.1862540933601324 3.992226130487146E-4 8.616315389540603E-6 7.467473337601856E-5 6.318631285663109E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ATCTCGA 23875 0.0 47.32258 1 TCTCGAG 29940 0.0 37.67952 2 CTCGAGG 36760 0.0 30.471676 3 TCGAGGG 41360 0.0 26.923199 4 TATCTCG 11525 0.0 22.513556 2 GATCCGG 30840 0.0 22.032938 1 ATCCGGC 36555 0.0 18.606625 2 AGGGCGC 60445 0.0 18.412191 7 GGGCGCG 63655 0.0 17.631416 8 CGAGGGC 64220 0.0 17.513569 5 GAGGGCG 67840 0.0 16.543629 6 ATATCTC 16960 0.0 15.981453 1 GGATCCG 22170 0.0 15.3928385 3 ATGGATC 23585 0.0 14.864148 1 TCCGGCG 47675 0.0 14.4541 3 GCGCCGT 48745 0.0 13.797327 9 CGGCGCG 49555 0.0 13.648206 5 TATGGAT 22085 0.0 13.493909 2 GGCGCGC 108950 0.0 13.342526 9 CGTCGAC 46495 0.0 12.438284 12-13 >>END_MODULE