FastQCFastQC Report
Wed 25 May 2016
SRR491066_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR491066_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42110391
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCTCGA247100.048.1201321
TCTCGAG329400.036.0899282
CTCGAGG375550.031.4297033
TCGAGGG440300.026.7216434
GATCCGG376000.024.377851
ATCCGGC438300.020.8919162
TATCTCG117050.020.4813982
AGGGCGC650200.018.1285047
CGAGGGC692400.017.1733955
GGGCGCG696400.016.9461448
GGATCCG259400.016.635412
TCCGGCG561850.016.3731333
GAGGGCG778550.015.2907236
GCGCCGT615950.014.8102719
CGGCGCG616350.014.739995
TATGGAT246400.013.8692992
TGGATCC321400.013.7032721
ATGGATC268200.013.5441831
CCGGCGC691950.013.2943514
CGTCGAC592900.012.96039512-13
Produced by FastQC (version 0.11.5)