FastQCFastQC Report
Wed 25 May 2016
SRR491065_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR491065_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42010797
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1803650.4293301076863645No Hit
ATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTT499050.11879089082742228No Hit
GATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT481900.11470860693264162No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCTCGA321300.043.7006261
TCTCGAG401500.035.0636142
CTCGAGG480200.029.2095343
TATCTCG205250.028.9244792
GATCCGG544200.027.5564371
ATATCTC232550.026.1828841
TCGAGGG558700.024.9872514
ATCCGGC635100.023.5802862
TATGGAT277250.022.7523542
TCCGGCG787150.019.1389663
CGAGGGC807100.017.4174185
ATGGATC361600.017.3410473
GGATCCG366900.016.6401085
AGGGCGC849100.016.3342387
CGGCGCG925200.016.1847085
GCGCCGT943150.015.8516329
CCGGCGC961600.015.6910244
TGGATCC414400.015.1426624
GGGCGCG921750.015.1233878
GAGGGCG974700.014.4125856
Produced by FastQC (version 0.11.5)