FastQCFastQC Report
Wed 25 May 2016
SRR491045_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR491045_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20336393
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA254270.12503200542987147No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCTCGA131900.047.5499081
TCTCGAG158200.039.6002542
CTCGAGG203200.030.717453
TCGAGGG238400.026.023874
GATCCGG204300.023.5015091
TATCTCG66100.022.6640662
ATCCGGC238050.020.102712
CGAGGGC323950.019.3108585
AGGGCGC339700.018.2138967
GGGCGCG372350.016.654618
GGATCCG145650.016.1873993
GAGGGCG393500.015.9385956
TCCGGCG308050.015.8560863
ATATCTC98150.015.7956271
GCGCCGT314250.015.2819229
ATGGATC158550.015.1710961
CGGCGCG327700.014.64703755
CCGGCGC355550.013.5525814
CGTCGAC298750.013.29510912-13
TGGATCC184600.013.2338091
Produced by FastQC (version 0.11.5)