FastQCFastQC Report
Wed 25 May 2016
SRR491043_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR491043_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29723853
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA510760.17183505785740497No Hit
GATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT323170.10872412805971016No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCTCGA216500.049.9989431
TCTCGAG263500.040.905442
CTCGAGG338500.031.7902323
TCGAGGG393100.027.3018534
TATCTCG103300.026.369792
GATCCGG306650.023.9623831
CGAGGGC524750.020.4254745
ATCCGGC363550.020.3726022
ATATCTC149100.018.748891
AGGGCGC570550.018.6709127
GGGCGCG626050.017.1132058
GAGGGCG635800.016.9469956
TCCGGCG454350.016.3960743
GCGCCGT472800.015.6294599
GGATCCG220400.015.1738463
CGGCGCG497300.014.8377035
ATGGATC236450.014.7037681
CCGGCGC539150.013.7905084
CGTCGAC454100.013.586412-13
TATGGAT250250.013.5313212
Produced by FastQC (version 0.11.5)