FastQCFastQC Report
Wed 25 May 2016
SRR491042_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR491042_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19547616
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA281500.14400733061259235No Hit
GATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT210540.10770622872886393No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCTCGA166250.052.7743871
TCTCGAG199900.043.9389952
CTCGAGG256750.034.0914653
TCGAGGG298250.029.2534164
TATCTCG76300.025.3626142
GATCCGG216550.023.663261
CGAGGGC399050.021.8405275
ATATCTC96850.020.7079091
ATCCGGC250050.020.5312142
AGGGCGC423300.020.4568357
GGGCGCG454650.019.0876628
GAGGGCG466800.018.6609786
TCCGGCG316300.016.211893
GGATCCG152750.015.8627583
ATGGATC162900.015.396721
GCGCCGT335300.015.1387929
CGGCGCG351750.014.4579525
GGCGCGC784300.014.1714779
CCGGCGC367750.013.9054974
TGGATCC183700.013.5515212
Produced by FastQC (version 0.11.5)