FastQCFastQC Report
Wed 25 May 2016
SRR491041_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR491041_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24407335
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCTCGA140400.046.33171
TCTCGAG179000.036.446622
CTCGAGG231850.028.0287363
GATCCGG233200.027.1894721
TCGAGGG268100.024.0813774
ATCCGGC280900.022.790412
GGATCCG161700.019.1127822
TATCTCG78450.017.9612432
TCCGGCG355800.017.8816623
CGAGGGC374600.017.4356945
GCGCCGT368000.017.122879
CGGCGCG377400.016.8211125
AGGGCGC394350.016.4084577
GGGCGCG427800.015.180388
CCGGCGC425350.015.1454814
TGGATCC213300.014.9071071
GAGGGCG464300.013.9969186
CGTCGAC351500.013.85526112-13
ATATCTC111300.013.2082341
TCGACTT371400.013.19498114-15
Produced by FastQC (version 0.11.5)