##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR491041_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	24407335
Sequences flagged as poor quality	0
Sequence length	100
%GC	45
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.61771467470742	33.0	31.0	34.0	30.0	34.0
2	31.895073018008727	34.0	31.0	34.0	30.0	34.0
3	31.96442827535247	34.0	31.0	34.0	30.0	34.0
4	35.34753802494209	37.0	35.0	37.0	33.0	37.0
5	35.1260320309448	37.0	35.0	37.0	32.0	37.0
6	35.05236048917262	37.0	35.0	37.0	32.0	37.0
7	35.00098912888277	37.0	35.0	37.0	32.0	37.0
8	34.966770890799836	37.0	35.0	37.0	32.0	37.0
9	36.58628510650589	39.0	37.0	39.0	32.0	39.0
10-11	36.52302975724306	39.0	37.0	39.0	32.0	39.0
12-13	36.44248067230609	39.0	37.0	39.0	32.0	39.0
14-15	37.68239291590008	40.0	38.0	41.0	32.0	41.0
16-17	37.58279771224511	40.0	38.0	41.0	32.0	41.0
18-19	37.486721676086304	40.0	38.0	41.0	32.0	41.0
20-21	37.39620077325115	40.0	38.0	41.0	32.0	41.0
22-23	37.2271070971083	40.0	38.0	41.0	31.0	41.0
24-25	37.05509585540576	40.0	37.0	41.0	31.0	41.0
26-27	36.78702687122539	40.0	37.0	41.0	30.0	41.0
28-29	36.6443560101912	40.0	36.5	41.0	30.0	41.0
30-31	36.481224250824596	40.0	36.0	41.0	30.0	41.0
32-33	36.47852114948232	40.0	36.0	41.0	30.0	41.0
34-35	36.28857198051323	40.0	36.0	41.0	29.5	41.0
36-37	36.089150331242635	40.0	36.0	41.0	28.5	41.0
38-39	35.86124124161856	40.0	35.0	41.0	27.5	41.0
40-41	35.577658662856884	40.0	35.0	41.0	26.0	41.0
42-43	35.218338093855806	39.0	35.0	41.0	24.5	41.0
44-45	34.85000816352953	39.0	35.0	41.0	23.0	41.0
46-47	34.44614831975716	39.0	34.0	41.0	20.0	41.0
48-49	34.06151695791449	39.0	33.5	40.5	16.5	41.0
50-51	33.71207423915802	38.0	33.0	40.0	12.5	41.0
52-53	33.41445329856783	38.0	33.0	40.0	8.5	41.0
54-55	32.935043092578525	38.0	32.5	40.0	4.5	41.0
56-57	32.59612413645324	38.0	32.0	40.0	2.0	41.0
58-59	32.18790941329728	37.0	31.0	40.0	2.0	41.0
60-61	31.747630968313416	36.5	31.0	40.0	2.0	41.0
62-63	31.229375288207418	36.0	30.0	39.5	2.0	41.0
64-65	30.701100980504428	35.5	29.5	39.0	2.0	41.0
66-67	30.17017111864118	35.0	29.0	39.0	2.0	40.0
68-69	29.597172448364397	35.0	28.0	38.0	2.0	40.0
70-71	28.99683625844444	34.5	26.5	37.5	2.0	40.0
72-73	28.422349531401114	34.0	26.0	37.0	2.0	39.0
74-75	27.84159196405507	34.0	25.5	36.0	2.0	39.0
76-77	27.10993285420141	33.0	25.0	35.0	2.0	38.0
78-79	27.14581718979151	34.0	25.0	35.0	2.0	37.5
80-81	26.730679711652257	33.5	24.0	35.0	2.0	37.0
82-83	26.194445419788764	33.0	21.5	35.0	2.0	37.0
84-85	25.61600348829563	33.0	19.0	35.0	2.0	36.0
86-87	25.00178661455665	32.0	14.0	35.0	2.0	36.0
88-89	24.453333393424558	32.0	4.5	35.0	2.0	35.0
90-91	23.828384274645305	31.5	2.0	35.0	2.0	35.0
92-93	23.195156947696255	31.0	2.0	34.0	2.0	35.0
94-95	22.483339557555137	31.0	2.0	34.0	2.0	35.0
96-97	21.758864251258892	30.0	2.0	34.0	2.0	35.0
98-99	21.04549935500947	30.0	2.0	34.0	2.0	35.0
100	19.900293088122893	27.0	2.0	34.0	2.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	-0.046233551482572466
1101	2	-0.011397506249814171
1101	3	0.005339296895584766
1101	4	0.06553619715265313
1101	5	0.08441472261392136
1101	6	0.081167524732912
1101	7	0.0758388330622708
1101	8	0.07154589375652876
1101	9	0.04879115721739424
1101	10-11	0.05463953828252954
1101	12-13	0.027538282174084827
1101	14-15	-0.025375774455035582
1101	16-17	-0.03126714031272826
1101	18-19	-0.021224513209602947
1101	20-21	-0.06529544005316978
1101	22-23	-0.05555133027474568
1101	24-25	-0.09075163245349671
1101	26-27	-0.20498661187426137
1101	28-29	-0.22539703822135948
1101	30-31	-0.23136941437875436
1101	32-33	-0.27530609211029144
1101	34-35	-0.3009757737289007
1101	36-37	-0.30484996998385583
1101	38-39	-0.3695429089371274
1101	40-41	-0.4295367948267028
1101	42-43	-0.4676472991783953
1101	44-45	-0.5796494705351094
1101	46-47	-0.6036990052459288
1101	48-49	-0.7144224187392823
1101	50-51	-0.7710538978116901
1101	52-53	-0.8080539044267852
1101	54-55	-0.7801513619796339
1101	56-57	-0.8542133173173347
1101	58-59	-0.9581862436440005
1101	60-61	-1.048281919393741
1101	62-63	-1.1196978810821996
1101	64-65	-1.156628820413335
1101	66-67	-1.2288213041430076
1101	68-69	-1.261791088328824
1101	70-71	-1.3213441993262407
1101	72-73	-1.3383680367912945
1101	74-75	-1.4788167278380975
1101	76-77	-1.3652349723153634
1101	78-79	-1.3312248605265893
1101	80-81	-1.4289300385868344
1101	82-83	-1.449447983482088
1101	84-85	-1.5135067686179902
1101	86-87	-1.513759985526729
1101	88-89	-1.560423544574494
1101	90-91	-1.5914460929906902
1101	92-93	-1.636271594489635
1101	94-95	-1.6892381276315085
1101	96-97	-1.7130483804895498
1101	98-99	-1.6220478837468235
1101	100	-1.4513489408728546
1102	1	0.07525586653807181
1102	2	0.10851153592043872
1102	3	0.05554122738323386
1102	4	0.09370759871126921
1102	5	0.07592063942156102
1102	6	0.11899321902283333
1102	7	0.13655723640964368
1102	8	0.1081820798690245
1102	9	0.12086147733510444
1102	10-11	0.0847299474815344
1102	12-13	0.056801210625060605
1102	14-15	0.04964204237410286
1102	16-17	0.04040959312526127
1102	18-19	0.04744037414532443
1102	20-21	0.015159610732943918
1102	22-23	0.04761532017526804
1102	24-25	0.011986161158624498
1102	26-27	-0.026431678676935633
1102	28-29	-0.011016160988660317
1102	30-31	-0.061259925652194624
1102	32-33	-0.09707102571567106
1102	34-35	-0.2153928388413604
1102	36-37	-0.12946075549265146
1102	38-39	-0.06205935596264567
1102	40-41	-0.12829500772217273
1102	42-43	-0.21889326271044496
1102	44-45	-0.29875834731630135
1102	46-47	-0.3631535343316017
1102	48-49	-0.43893732669336316
1102	50-51	-0.379225820249637
1102	52-53	-0.4193883660101889
1102	54-55	-0.4458318119030338
1102	56-57	-0.47233946547454764
1102	58-59	-0.5406907849630755
1102	60-61	-0.5319136306381758
1102	62-63	-0.6417947293218766
1102	64-65	-0.6862077506730309
1102	66-67	-0.8009223747860688
1102	68-69	-0.8349932240917113
1102	70-71	-0.9694293016608917
1102	72-73	-0.8930553688827843
1102	74-75	-1.0471455577551616
1102	76-77	-0.9256043741937461
1102	78-79	-0.9307604680387485
1102	80-81	-0.9522367978950683
1102	82-83	-1.0287802672065496
1102	84-85	-1.0996919899747084
1102	86-87	-1.152408608387418
1102	88-89	-1.2233558943411822
1102	90-91	-1.2085606150775767
1102	92-93	-1.2868594310538484
1102	94-95	-1.3527566914103666
1102	96-97	-1.4428738552989948
1102	98-99	-1.3326019843281003
1102	100	-1.199243781892715
1103	1	-0.027642336418821856
1103	2	0.02403577277314284
1103	3	0.029791909020143947
1103	4	0.0693090719245717
1103	5	0.09678661925240561
1103	6	0.08210763095537743
1103	7	0.09734846346683668
1103	8	0.08608320758438737
1103	9	0.08710062161057408
1103	10-11	0.0074015686038819695
1103	12-13	0.1025848221336858
1103	14-15	0.15941431569662967
1103	16-17	0.1510502695481435
1103	18-19	0.15476188934340485
1103	20-21	0.09736568984512672
1103	22-23	0.1757561515783408
1103	24-25	0.13151962831896924
1103	26-27	0.21800874529043313
1103	28-29	0.12368717475734314
1103	30-31	0.10124857522754382
1103	32-33	0.1496809426914396
1103	34-35	0.07626793626528894
1103	36-37	-8.382077582567149E-4
1103	38-39	0.07938658287077516
1103	40-41	0.11653691042103276
1103	42-43	0.061070553849333464
1103	44-45	-0.04813936543779107
1103	46-47	0.04672110728311196
1103	48-49	-0.04424904060626034
1103	50-51	0.06308722063904071
1103	52-53	-0.009341775303475686
1103	54-55	-0.017629939102640435
1103	56-57	-0.12555491313769807
1103	58-59	-0.14146009461868658
1103	60-61	-0.21943492383844543
1103	62-63	-0.15502668792982277
1103	64-65	-0.20585733744673362
1103	66-67	-0.3216652212592912
1103	68-69	-0.26272548345317404
1103	70-71	-0.3969750860910395
1103	72-73	-0.3196218759413654
1103	74-75	-0.4108954014299613
1103	76-77	-0.3561600338502906
1103	78-79	-0.3130498488498006
1103	80-81	-0.3265745502230857
1103	82-83	-0.360709292742051
1103	84-85	-0.4454115158544312
1103	86-87	-0.5122949606127563
1103	88-89	-0.5398927763849741
1103	90-91	-0.5661940597301758
1103	92-93	-0.5684487415873605
1103	94-95	-0.6268712732995638
1103	96-97	-0.6294566463412359
1103	98-99	-0.6185630468455869
1103	100	-0.5690931608424599
1104	1	0.0723887262222398
1104	2	0.0801907802590911
1104	3	0.08273468770524417
1104	4	0.09217510028182829
1104	5	0.086599305830795
1104	6	0.10885974373204022
1104	7	0.11117447578735806
1104	8	0.10287061423853316
1104	9	0.13397901088970343
1104	10-11	0.11319015563838519
1104	12-13	0.09889282788304143
1104	14-15	0.1370203946895856
1104	16-17	0.1144949909451185
1104	18-19	0.14852758262709642
1104	20-21	0.15427786188011083
1104	22-23	0.1833952432405752
1104	24-25	0.17121510932756934
1104	26-27	0.17461048096632936
1104	28-29	0.15416086548933094
1104	30-31	0.11739708726473452
1104	32-33	0.14340229313794595
1104	34-35	0.07878965109016178
1104	36-37	0.12170297682255438
1104	38-39	0.1284637855581252
1104	40-41	0.12998175772023046
1104	42-43	0.19428652646250555
1104	44-45	0.13650964288458312
1104	46-47	0.14216391847605792
1104	48-49	0.16682956378759428
1104	50-51	0.1679877951362343
1104	52-53	0.2014360907049948
1104	54-55	0.25468631995597235
1104	56-57	0.2601383687885388
1104	58-59	0.28339718544001613
1104	60-61	0.28212522740186685
1104	62-63	0.21490407145056878
1104	64-65	0.2842837937485889
1104	66-67	0.25265945994659944
1104	68-69	0.24674557866525504
1104	70-71	0.16712350454544733
1104	72-73	0.168888068983172
1104	74-75	0.14236882222453673
1104	76-77	0.16940596642026762
1104	78-79	0.1883960875054811
1104	80-81	0.16348659711396607
1104	82-83	0.13095672398279845
1104	84-85	0.18619808447074604
1104	86-87	0.24390223849668047
1104	88-89	0.1334278205711854
1104	90-91	0.06192576246019854
1104	92-93	0.04942167010285203
1104	94-95	0.04952445230848568
1104	96-97	0.060783633501028334
1104	98-99	0.08439867118394773
1104	100	0.11312757182966493
1105	1	0.05161709065220066
1105	2	0.0522615079710107
1105	3	0.008850485203630853
1105	4	0.03726302507271839
1105	5	0.04867333310605915
1105	6	0.049464666643629585
1105	7	0.051440537615739856
1105	8	0.025121390023585377
1105	9	0.020835395125672562
1105	10-11	0.07134478468643835
1105	12-13	0.059622382376119276
1105	14-15	0.08431247993691926
1105	16-17	0.1051398441405027
1105	18-19	0.10304631440530443
1105	20-21	-0.024808136637439304
1105	22-23	0.052561123154326594
1105	24-25	0.1064203937001551
1105	26-27	0.048425983379608795
1105	28-29	0.05537190863775976
1105	30-31	0.055207551180679104
1105	32-33	0.11034423579020114
1105	34-35	0.12127875684803513
1105	36-37	0.12284857554018203
1105	38-39	0.13611163517062153
1105	40-41	0.1228101110406854
1105	42-43	0.100727805926482
1105	44-45	0.09632229232950351
1105	46-47	0.1564508783256926
1105	48-49	0.16101397928380123
1105	50-51	0.2286748002969361
1105	52-53	0.203532300678539
1105	54-55	0.25109769878286414
1105	56-57	0.2616348845984575
1105	58-59	0.2124986369613282
1105	60-61	0.13984918800513313
1105	62-63	0.1534561483539001
1105	64-65	0.18401062054774187
1105	66-67	0.20726446005600963
1105	68-69	0.25018708146392044
1105	70-71	0.23197536780802608
1105	72-73	0.13339473432109372
1105	74-75	0.061372382326709385
1105	76-77	0.10345333012913827
1105	78-79	0.1477881938930352
1105	80-81	0.1483197552824862
1105	82-83	0.1937458675853918
1105	84-85	0.18806000367110798
1105	86-87	0.16904102106406071
1105	88-89	0.1092519303769599
1105	90-91	0.15529604302918543
1105	92-93	0.15450143661190197
1105	94-95	0.1758800455441083
1105	96-97	0.16659060497574174
1105	98-99	0.18174374628560486
1105	100	0.17381830797017983
1106	1	-0.06334436562621093
1106	2	-0.0383885983935599
1106	3	-0.059980821631327785
1106	4	-0.05374804564307567
1106	5	-0.03437706469694746
1106	6	-0.07366690131845388
1106	7	-0.03592225863030052
1106	8	-0.04324588758235137
1106	9	-0.052702776180865385
1106	10-11	-0.04676604225434744
1106	12-13	-0.08299551547825246
1106	14-15	-0.08617834157351467
1106	16-17	-0.08343274525874023
1106	18-19	-0.034802717343012546
1106	20-21	-0.19828805668402083
1106	22-23	-0.0603186161410747
1106	24-25	-0.044842484838859775
1106	26-27	-0.07061516870491147
1106	28-29	-0.011799114200428562
1106	30-31	0.017072196993666466
1106	32-33	0.037756068608977955
1106	34-35	-0.02767940299849414
1106	36-37	-0.05150697996328546
1106	38-39	-0.040059994878703264
1106	40-41	-0.049530376920237984
1106	42-43	-0.011049986165545533
1106	44-45	0.01662987289406459
1106	46-47	0.014771803564300967
1106	48-49	0.07474561748277608
1106	50-51	0.00619157261488823
1106	52-53	-0.013897835253054325
1106	54-55	0.08892678127639897
1106	56-57	0.10935975979831625
1106	58-59	0.10658648692868411
1106	60-61	0.14902693917062493
1106	62-63	0.13050011017358187
1106	64-65	0.16597213558801727
1106	66-67	0.1537053935951178
1106	68-69	0.2016572723648622
1106	70-71	0.21637308935242316
1106	72-73	0.2070056508335334
1106	74-75	0.15624381682780708
1106	76-77	0.17995218261824775
1106	78-79	0.24908447981086113
1106	80-81	0.26545997750899986
1106	82-83	0.30897211786572143
1106	84-85	0.2889390323658958
1106	86-87	0.34136116622829604
1106	88-89	0.29569021741433943
1106	90-91	0.321758427346726
1106	92-93	0.2659298987221774
1106	94-95	0.2775717748218547
1106	96-97	0.34985404778054274
1106	98-99	0.28242614785654396
1106	100	0.12263989142146414
1107	1	-0.25289626558557643
1107	2	-0.20977802377019472
1107	3	-0.16723957306573212
1107	4	-0.18818907320013523
1107	5	-0.2155587781599806
1107	6	-0.23142957670329167
1107	7	-0.3494747979145032
1107	8	-0.32004289145639575
1107	9	-0.38039621182643657
1107	10-11	-0.31093977216230684
1107	12-13	-0.2727066408348904
1107	14-15	-0.25206658778410684
1107	16-17	-0.30397741440798853
1107	18-19	-0.30223951615862177
1107	20-21	-0.48207296843077785
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2207	92-93	0.7340194577768813
2207	94-95	0.6347894437212211
2207	96-97	0.7276101414255045
2207	98-99	0.9017484278627705
2207	100	0.8565605942204293
2208	1	-0.35447349438195985
2208	2	-0.3189155587716215
2208	3	-0.29426104556728205
2208	4	-0.31564393304912386
2208	5	-0.37211599123502737
2208	6	-0.5346854624944726
2208	7	-0.7173730956079609
2208	8	-0.4665010914979604
2208	9	-0.4744686141872947
2208	10-11	-0.5425662047237267
2208	12-13	-0.4661627442017462
2208	14-15	-0.46126697609321354
2208	16-17	-0.4971828041832467
2208	18-19	-0.5500648702310684
2208	20-21	-0.4230038993601042
2208	22-23	-0.5137272989801431
2208	24-25	-0.6498804464878205
2208	26-27	-0.49336701557135143
2208	28-29	-0.6040143910479543
2208	30-31	-0.3489412198274948
2208	32-33	-0.5091103300271058
2208	34-35	-0.44102304001161485
2208	36-37	-0.3057971931594139
2208	38-39	-0.6795907929848752
2208	40-41	-0.39510064196022654
2208	42-43	-0.6167091328429422
2208	44-45	-0.4429867367733351
2208	46-47	-0.6180986853378982
2208	48-49	-0.2762174283193559
2208	50-51	-0.10795566672948809
2208	52-53	-0.07544607030737893
2208	54-55	-1.0047476154518336
2208	56-57	-0.5046814642764232
2208	58-59	-0.40905671428367896
2208	60-61	-0.08025325401238703
2208	62-63	0.09593481774934531
2208	64-65	-0.1091052901294951
2208	66-67	0.34853841460277835
2208	68-69	0.4138726382529683
2208	70-71	0.04068570103346758
2208	72-73	0.3444223730193876
2208	74-75	0.6125623589129141
2208	76-77	0.5878466195122947
2208	78-79	0.49800565574577504
2208	80-81	0.6747361364935962
2208	82-83	0.6212036376630579
2208	84-85	0.5783305414059541
2208	86-87	0.66943743191446
2208	88-89	1.1924869631468482
2208	90-91	1.2631410758069244
2208	92-93	1.53580813370127
2208	94-95	1.6480322071093063
2208	96-97	1.8629441348351463
2208	98-99	2.0423081963652443
2208	100	1.8041769559739969
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	330670.0
3	114020.0
4	74657.0
5	61550.0
6	62936.0
7	65980.0
8	73803.0
9	84470.0
10	95406.0
11	106593.0
12	124990.0
13	165532.0
14	214579.0
15	247972.0
16	302763.0
17	302454.0
18	271746.0
19	241431.0
20	242860.0
21	254932.0
22	271529.0
23	293159.0
24	318731.0
25	351663.0
26	392030.0
27	442653.0
28	504766.0
29	575531.0
30	665545.0
31	774480.0
32	916636.0
33	1118839.0
34	1423089.0
35	1902645.0
36	2715134.0
37	3942565.0
38	3681970.0
39	676832.0
40	194.0
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	22.390887821222595	26.799628062629534	27.86199312624668	22.947490989901194
2	24.78467179971977	27.74692038127691	25.094998699849526	22.373409119153788
3	24.18221961388058	26.670796328487818	25.218071218761835	23.928912838869767
4	24.359858472679207	26.219896712343527	25.596171854369693	23.824072960607573
5	24.345275713223096	26.28020633961061	25.792103070654786	23.582414876511507
6	24.159701483608373	26.392215057284673	25.30345678119995	24.144626677907006
7	24.261185182423297	26.704707414504092	25.64771738099428	23.38639002207833
8	23.75508786790696	26.547160128456937	26.014135638997526	23.683616364638578
9	23.526598234338067	26.279737734809444	26.322535600620352	23.871128430232144
10-11	23.472965496703033	26.445178815975943	26.365289926968682	23.716565760352346
12-13	23.20669082833266	26.532047670461644	26.661272974240983	23.599988526964705
14-15	22.98083071170562	26.723851897519772	27.105210606853074	23.19010678392153
16-17	22.866172157427464	26.620507926986697	27.353606956843436	23.159712958742404
18-19	22.477040692908908	26.574035677750647	27.980094280393423	22.968829348947022
20-21	22.19116636868865	26.347091955445944	28.63867259205058	22.823069083814826
22-23	22.283695385980216	26.2546463450337	28.96984244226779	22.491815826718295
24-25	22.170717083540044	26.174256552273494	29.323510945647254	22.331515418539208
26-27	22.22293087470214	26.245928011256687	29.313182644131462	22.21795846990971
28-29	22.24638807852605	26.30320223391578	29.309548868710895	22.140860818847273
30-31	22.216596302371176	26.25363877385874	29.29705309646442	22.232711827305664
32-33	22.220501151590895	26.334370134966157	29.214585934275718	22.230542779167234
34-35	22.282241488592298	26.373147946255592	29.203721710830326	22.14088885432178
36-37	22.252161069412672	26.346617007344715	29.15345810987639	22.247763813366227
38-39	22.30997515546573	26.42350922975158	28.993594322852108	22.27292129193059
40-41	22.38593734797945	26.53711667998588	28.81030170828848	22.266644263746194
42-43	22.484004980530166	26.633444078720043	28.436152635233782	22.44639830551601
44-45	22.57811796749191	26.839639077584398	28.048732007910477	22.533510947013212
46-47	22.77839732585611	26.998120031837793	27.62869667162636	22.594785970679737
48-49	22.84014621193636	27.026788838679806	27.355235644581118	22.777829304802715
50-51	22.845020775891587	27.13245857929614	27.18759487114878	22.834925773663496
52-53	22.969176203238902	27.139745390013676	27.060333937440305	22.830744469307117
54-55	22.994710410991306	27.132249848675816	26.97652482697237	22.896514913360512
56-57	22.978293717345462	27.188707644844207	26.966118416993684	22.866880220816647
58-59	23.026730237978484	27.20072565886042	26.953818858646727	22.818725244514372
60-61	22.987484232409543	27.189241039994883	26.91729863716961	22.905976090425973
62-63	23.053634863867437	27.262930312424267	26.868181048728022	22.815253774980278
64-65	23.11906170485429	27.315980439085063	26.80118425680485	22.763773599255803
66-67	23.128126571353754	27.33093719174829	26.706799067115323	22.834137169782633
68-69	23.117611667319775	27.46380534820926	26.608287481177907	22.810295503293062
70-71	23.207021968051798	27.534354526556264	26.547452674803786	22.71117083058815
72-73	23.171844494939144	27.60666135568132	26.4155413603296	22.80595278904994
74-75	23.249066336580558	27.715118547318436	26.34948738404476	22.68632773205625
76-77	23.07019028182476	27.868194906756123	26.236912184784806	22.82470262663431
78-79	23.089167403022586	27.917901306428362	26.150970869936735	22.84196042061232
80-81	23.1365104254764	28.003231081451414	26.028650801611747	22.831607691460444
82-83	23.182356737210608	28.098778388726274	25.92210336666646	22.79676150739666
84-85	23.158306112521856	28.143024095886155	25.822066730861387	22.876603060730602
86-87	23.162010933964016	28.273616062295755	25.691709419667262	22.872663584072967
88-89	23.284187727983657	28.304732185558862	25.54274131171698	22.868338774740494
90-91	23.252379641387382	28.3373879738945	25.443934429212145	22.96629795550597
92-93	23.307756114606676	28.462325311038217	25.325079207217883	22.90483936713722
94-95	23.324062869300267	28.509379204934444	25.232024182479513	22.934533743285783
96-97	23.293257064375826	28.585035199599613	25.065964357991817	23.05574337803275
98-99	23.331753163111323	28.669292826398006	24.942356976002863	23.056597034487805
100	23.986131092944106	28.483671270172305	24.168406658573453	23.361790978310136
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	992.0
1	672.0
2	338.5
3	338.0
4	382.5
5	452.0
6	519.0
7	593.5
8	706.5
9	862.0
10	1116.5
11	1517.0
12	1980.5
13	2789.0
14	4913.5
15	7029.0
16	9846.0
17	11989.5
18	13830.0
19	18596.5
20	25096.0
21	32973.5
22	43144.5
23	56068.5
24	71362.0
25	89855.0
26	112261.0
27	138777.5
28	170335.0
29	204134.0
30	238876.5
31	279083.0
32	326110.0
33	377675.0
34	432590.5
35	494135.5
36	564456.0
37	646500.5
38	722666.0
39	770680.0
40	811767.0
41	866785.5
42	943916.5
43	1014564.5
44	1069740.0
45	1104560.0
46	1088853.5
47	1033684.5
48	979633.0
49	958335.5
50	929774.5
51	873248.5
52	796407.0
53	707103.5
54	640191.0
55	591000.0
56	532473.0
57	475054.0
58	432351.0
59	393067.5
60	347892.0
61	304208.0
62	266198.5
63	230346.0
64	198335.5
65	170105.5
66	144172.5
67	121157.0
68	100993.0
69	83144.0
70	67736.0
71	55452.0
72	45135.0
73	36595.0
74	29726.0
75	23158.5
76	17522.0
77	13633.0
78	10902.0
79	8321.0
80	5617.5
81	3431.5
82	2100.5
83	1271.5
84	773.5
85	454.0
86	259.5
87	163.0
88	110.0
89	60.5
90	37.5
91	29.0
92	16.5
93	9.5
94	5.0
95	3.0
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.04162273349384519
3	7.00609058711244E-4
4	0.0030318754587504125
5	0.0
6	0.009738875628986121
7	0.004301985448226937
8	0.013123104181591313
9	0.017408701113825004
10-11	0.008968615377303585
12-13	0.009361939761141476
14-15	0.010203899770294463
16-17	0.016730626264604472
18-19	0.02707997411433899
20-21	0.018551800104353876
22-23	0.02195651430195062
24-25	0.010416950478206654
26-27	0.010834857636034414
28-29	0.02481631034277196
30-31	0.022122448026382233
32-33	0.01606279423787972
34-35	0.2794487804588252
36-37	0.349685862876877
38-39	0.3799329177069106
40-41	0.3649599597825817
42-43	0.3681004091597874
44-45	0.38930305172604873
46-47	0.40865788911407164
48-49	0.20970745064956908
50-51	0.01708707648745756
52-53	0.008870284281344112
54-55	0.03506527853204785
56-57	0.055401378315166325
58-59	0.030482639747436584
60-61	0.01490535529585676
62-63	0.028309112813832398
64-65	0.01713419347093814
66-67	0.02296440803553522
68-69	0.008913304135826382
70-71	0.013260358003034744
72-73	0.01054191291265515
74-75	0.013102618536599756
76-77	0.025025263921685838
78-79	0.030058586896111354
80-81	0.010255113882773354
82-83	0.020209088784170822
84-85	0.01630042771978178
86-87	0.01271134271726102
88-89	0.004578541655612954
90-91	0.015632595693057024
92-93	0.01549739043611275
94-95	0.009128403408237729
96-97	0.0047629124605369656
98-99	0.007114664505567691
100	0.010357542107731139
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	2.4407335E7
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	50.35100155706282
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.7661139752614	41.17005732083363
2	10.577180218017602	10.651432352534767
3	3.0871052105673997	4.6631651779228775
4	1.3854700056274867	2.7903920964245366
5	0.7351015008133774	1.8506548406026797
6	0.43786059366015173	1.3228031659895252
7	0.3287776886849159	1.1588000140431203
8	0.2223191224407138	0.8955192384141778
9	0.16737347154058269	0.7584679733535801
>10	1.0418570136530525	9.456872120268132
>50	0.08000840919257239	2.9141181712319475
>100	0.15336650187605105	16.07087286299539
>500	0.016632652337713924	5.288879911521997
>1k	7.196948907960912E-4	0.48528121201310376
>5k	8.952541414076753E-5	0.3537064754038908
>10k+	2.441602203835661E-5	0.16897706644652039
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	4.097128998311368E-6	0.0	0.0	0.0
5	0.0	1.2291386994934104E-5	0.0	0.0	0.0
6	0.0	1.2291386994934104E-5	0.0	0.0	0.0
7	0.0	1.2291386994934104E-5	0.0	0.0	0.0
8	0.0	1.2291386994934104E-5	0.0	0.0	0.0
9	0.0	1.2291386994934104E-5	0.0	4.097128998311368E-6	0.0
10-11	6.145693497467052E-6	1.2291386994934104E-5	0.0	4.097128998311368E-6	4.097128998311368E-6
12-13	7.989401546707168E-5	1.6388515993245472E-5	0.0	4.097128998311368E-6	4.097128998311368E-6
14-15	2.9909041687672987E-4	2.0485644991556842E-5	0.0	1.024282249577842E-5	4.097128998311368E-6
16-17	6.514435107315075E-4	3.687416098480231E-5	0.0	1.433995149408979E-5	4.097128998311368E-6
18-19	0.0011328561680330933	4.506841898142505E-5	0.0	2.0485644991556842E-5	4.097128998311368E-6
20-21	0.0018191252752502475	4.7116983480580734E-5	0.0	2.0485644991556842E-5	4.097128998311368E-6
22-23	0.002716396525880437	5.326267697804779E-5	0.0	2.458277398986821E-5	4.097128998311368E-6
24-25	0.003867689774405932	5.5311241477203474E-5	0.0	2.867990298817958E-5	4.097128998311368E-6
26-27	0.005031274409926361	5.940837047551484E-5	0.0	2.867990298817958E-5	4.097128998311368E-6
28-29	0.005930594225055706	6.760262847213758E-5	0.0	3.072846748733526E-5	4.097128998311368E-6
30-31	0.006850399685176608	6.965119297129326E-5	4.097128998311368E-6	4.301985448226937E-5	4.097128998311368E-6
32-33	0.007774302274295821	7.579688646876031E-5	4.097128998311368E-6	4.916554797973642E-5	4.097128998311368E-6
34-35	0.008999343844790921	8.603970896453874E-5	4.097128998311368E-6	4.916554797973642E-5	4.097128998311368E-6
36-37	0.010230531108783487	8.603970896453874E-5	4.097128998311368E-6	5.5311241477203474E-5	4.097128998311368E-6
38-39	0.01136338727681658	9.833109595947283E-5	4.097128998311368E-6	5.735980597635916E-5	4.097128998311368E-6
40-41	0.012744119749247511	1.0652535395609558E-4	4.097128998311368E-6	5.940837047551484E-5	4.097128998311368E-6
42-43	0.014323562978096543	1.1471961195271831E-4	4.097128998311368E-6	6.145693497467052E-5	4.097128998311368E-6
44-45	0.016591323878661884	1.1881674095102968E-4	4.097128998311368E-6	6.145693497467052E-5	4.097128998311368E-6
46-47	0.01935074025902459	1.1881674095102968E-4	4.097128998311368E-6	6.145693497467052E-5	4.097128998311368E-6
48-49	0.022917291052054638	1.2086530545018536E-4	4.097128998311368E-6	6.145693497467052E-5	4.097128998311368E-6
50-51	0.02747944419167435	1.2496243444849674E-4	4.097128998311368E-6	6.145693497467052E-5	4.097128998311368E-6
52-53	0.03345510683571148	1.3110812794596378E-4	4.097128998311368E-6	6.145693497467052E-5	4.097128998311368E-6
54-55	0.040774627791194736	1.3520525694427516E-4	4.097128998311368E-6	6.555406397298189E-5	4.097128998311368E-6
56-57	0.05015295606832946	1.3520525694427516E-4	4.097128998311368E-6	7.169975747044893E-5	4.097128998311368E-6
58-59	0.061813385197523614	1.3520525694427516E-4	4.097128998311368E-6	7.374832196960462E-5	4.097128998311368E-6
60-61	0.07677200317035843	1.3725382144343085E-4	4.097128998311368E-6	7.579688646876031E-5	4.097128998311368E-6
62-63	0.09515991811477983	1.433995149408979E-4	4.097128998311368E-6	8.399114446538305E-5	4.097128998311368E-6
64-65	0.11796248955488176	1.4749664393920925E-4	4.097128998311368E-6	8.603970896453874E-5	4.097128998311368E-6
66-67	0.1454747107785426	1.4749664393920925E-4	4.097128998311368E-6	9.423396696116147E-5	4.097128998311368E-6
68-69	0.1792616850631173	1.5364233743667632E-4	4.097128998311368E-6	1.0037966045862851E-4	4.097128998311368E-6
70-71	0.22062834799456804	1.55690901935832E-4	4.097128998311368E-6	1.0447678945693989E-4	4.097128998311368E-6
72-73	0.2700581607946955	1.55690901935832E-4	4.097128998311368E-6	1.0652535395609558E-4	4.097128998311368E-6
74-75	0.32929240328778214	1.55690901935832E-4	4.097128998311368E-6	1.0652535395609558E-4	4.097128998311368E-6
76-77	0.40025058040953676	1.5978803093414339E-4	4.097128998311368E-6	1.1471961195271831E-4	4.097128998311368E-6
78-79	0.4832256368833385	1.659337244316104E-4	4.097128998311368E-6	1.1881674095102968E-4	4.097128998311368E-6
80-81	0.5799424640174766	1.7207941792907747E-4	4.097128998311368E-6	1.270109989476524E-4	6.145693497467052E-6
82-83	0.6914212469325307	1.7617654692738885E-4	4.097128998311368E-6	1.3315669244511947E-4	8.194257996622736E-6
84-85	0.8202390797684385	1.802736759257002E-4	4.097128998311368E-6	1.413509504417422E-4	1.024282249577842E-5
86-87	0.965580633854536	1.8641936942316725E-4	4.097128998311368E-6	1.4544807944005356E-4	1.2291386994934104E-5
88	1.0862021601293217	1.9256506292063432E-4	4.097128998311368E-6	1.4749664393920925E-4	1.2291386994934104E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATCTCGA	14040	0.0	46.3317	1
TCTCGAG	17900	0.0	36.44662	2
CTCGAGG	23185	0.0	28.028736	3
GATCCGG	23320	0.0	27.189472	1
TCGAGGG	26810	0.0	24.081377	4
ATCCGGC	28090	0.0	22.79041	2
GGATCCG	16170	0.0	19.112782	2
TATCTCG	7845	0.0	17.961243	2
TCCGGCG	35580	0.0	17.881662	3
CGAGGGC	37460	0.0	17.435694	5
GCGCCGT	36800	0.0	17.12287	9
CGGCGCG	37740	0.0	16.821112	5
AGGGCGC	39435	0.0	16.408457	7
GGGCGCG	42780	0.0	15.18038	8
CCGGCGC	42535	0.0	15.145481	4
TGGATCC	21330	0.0	14.907107	1
GAGGGCG	46430	0.0	13.996918	6
CGTCGAC	35150	0.0	13.855261	12-13
ATATCTC	11130	0.0	13.208234	1
TCGACTT	37140	0.0	13.194981	14-15
>>END_MODULE