FastQCFastQC Report
Wed 25 May 2016
SRR491040_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR491040_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26676696
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA315370.11821928772588629No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCTCGA180150.051.0708581
TCTCGAG222800.041.33792
CTCGAGG281450.032.598453
TCGAGGG320750.028.3993684
GATCCGG233000.023.341331
CGAGGGC450450.020.3474925
ATCCGGC272000.019.857062
AGGGCGC461800.019.67517
TATCTCG94200.019.244621
GGGCGCG495850.018.380838
GAGGGCG525450.017.380976
GGATCCG165850.016.169391
TCCGGCG351600.015.5845993
GCGCCGT354550.015.2561069
CGGCGCG368800.014.7433215
GGCGCGC838500.013.6856979
ATGGATC182300.013.3449221
CCGGCGC421650.013.00661854
CGTCGAC340500.012.55787812-13
ATATCTC137650.012.4534481
Produced by FastQC (version 0.11.5)