##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR491040_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	26676696
Sequences flagged as poor quality	0
Sequence length	100
%GC	45
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.614187304154907	33.0	31.0	34.0	30.0	34.0
2	31.895097466342907	34.0	31.0	34.0	30.0	34.0
3	31.966311232845328	34.0	31.0	34.0	30.0	34.0
4	35.35071929447335	37.0	35.0	37.0	33.0	37.0
5	35.13320457675868	37.0	35.0	37.0	32.0	37.0
6	35.059442893527745	37.0	35.0	37.0	32.0	37.0
7	35.006435954437535	37.0	35.0	37.0	32.0	37.0
8	34.97147116719402	37.0	35.0	37.0	32.0	37.0
9	36.5891372754707	39.0	37.0	39.0	32.0	39.0
10-11	36.52680511859489	39.0	37.0	39.0	32.0	39.0
12-13	36.446026168308094	39.0	37.0	39.0	32.0	39.0
14-15	37.68212808287803	40.0	38.0	41.0	32.0	41.0
16-17	37.582261067862376	40.0	38.0	41.0	32.0	41.0
18-19	37.4766562358397	40.0	38.0	41.0	32.0	41.0
20-21	37.382995086797855	40.0	38.0	41.0	32.0	41.0
22-23	37.21232942415358	40.0	37.5	41.0	31.0	41.0
24-25	37.04060553450847	40.0	37.0	41.0	31.0	41.0
26-27	36.774401035270635	40.0	37.0	41.0	30.0	41.0
28-29	36.63286988388667	40.0	36.5	41.0	30.0	41.0
30-31	36.467658626090724	40.0	36.0	41.0	30.0	41.0
32-33	36.46176717311619	40.0	36.0	41.0	30.0	41.0
34-35	36.27229608944076	40.0	36.0	41.0	29.5	41.0
36-37	36.06823187549163	40.0	35.5	41.0	28.5	41.0
38-39	35.84306596663995	40.0	35.0	41.0	27.0	41.0
40-41	35.575218985139685	40.0	35.0	41.0	26.0	41.0
42-43	35.23608945050766	39.0	35.0	41.0	24.5	41.0
44-45	34.88109749423242	39.0	35.0	41.0	23.5	41.0
46-47	34.479103915267466	39.0	34.0	41.0	20.5	41.0
48-49	34.093599428504945	39.0	34.0	40.5	16.5	41.0
50-51	33.746707500808945	38.0	33.0	40.0	13.5	41.0
52-53	33.44912385326879	38.0	33.0	40.0	8.5	41.0
54-55	32.97382425094922	38.0	32.5	40.0	6.5	41.0
56-57	32.63895350833552	38.0	32.0	40.0	2.0	41.0
58-59	32.233426414575476	37.0	31.5	40.0	2.0	41.0
60-61	31.796515505518375	36.5	31.0	40.0	2.0	41.0
62-63	31.28076019983884	36.0	30.5	39.5	2.0	41.0
64-65	30.754251444781616	35.5	29.5	39.0	2.0	41.0
66-67	30.22750596625609	35.0	29.0	39.0	2.0	40.5
68-69	29.65581335484724	35.0	28.0	38.5	2.0	40.0
70-71	29.055639705156892	34.5	27.5	37.5	2.0	40.0
72-73	28.481564677274875	34.0	26.0	37.0	2.0	39.5
74-75	27.902301975477023	34.0	25.5	36.0	2.0	39.0
76-77	27.17179713709674	33.0	25.0	35.5	2.0	38.0
78-79	27.208593635433715	34.0	25.0	35.5	2.0	37.5
80-81	26.790767173715967	33.5	24.0	35.0	2.0	37.0
82-83	26.252311024573658	33.0	22.0	35.0	2.0	37.0
84-85	25.673091094189473	33.0	19.0	35.0	2.0	36.0
86-87	25.05318630912914	32.0	14.5	35.0	2.0	36.0
88-89	24.49853861212798	32.0	6.0	35.0	2.0	35.5
90-91	23.871908593927824	31.5	2.0	35.0	2.0	35.0
92-93	23.23510463964503	31.0	2.0	34.0	2.0	35.0
94-95	22.52088541999354	31.0	2.0	34.0	2.0	35.0
96-97	21.794429190181575	30.0	2.0	34.0	2.0	35.0
98-99	21.076114485841874	30.0	2.0	34.0	2.0	35.0
100	19.91878120888734	27.0	2.0	34.0	2.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	-0.05398214734938378
1101	2	-0.0168083785158899
1101	3	0.028093278697667756
1101	4	0.04816963469026092
1101	5	0.0782258049517921
1101	6	0.08966640663969372
1101	7	0.07500040342283398
1101	8	0.06361588595122214
1101	9	0.05393157125843828
1101	10-11	0.04134123892542618
1101	12-13	0.02629836156376797
1101	14-15	-0.006714771327011704
1101	16-17	0.002829427581048094
1101	18-19	0.018377489199075114
1101	20-21	-0.05965761774447742
1101	22-23	-0.02087192307392627
1101	24-25	-0.05731722635135128
1101	26-27	-0.1501188600615464
1101	28-29	-0.16996785564393946
1101	30-31	-0.19544002977762176
1101	32-33	-0.22142056153840883
1101	34-35	-0.2572111289261869
1101	36-37	-0.27428328002679336
1101	38-39	-0.31074050798202535
1101	40-41	-0.3724187093307165
1101	42-43	-0.3683589383356818
1101	44-45	-0.5067251753276736
1101	46-47	-0.5369081417482278
1101	48-49	-0.6362919405360827
1101	50-51	-0.6793701573014417
1101	52-53	-0.7440391342655417
1101	54-55	-0.7173246446195165
1101	56-57	-0.755674813088369
1101	58-59	-0.8444847606452512
1101	60-61	-0.9591583096209142
1101	62-63	-1.057615540525184
1101	64-65	-1.054786556222865
1101	66-67	-1.1462871214367638
1101	68-69	-1.139749612758969
1101	70-71	-1.1972059750464865
1101	72-73	-1.2059239303049942
1101	74-75	-1.316653448863498
1101	76-77	-1.2127828950973765
1101	78-79	-1.1750566553918063
1101	80-81	-1.2883165727510502
1101	82-83	-1.3145194425537596
1101	84-85	-1.3771762226006956
1101	86-87	-1.3892640309579036
1101	88-89	-1.4474258880203799
1101	90-91	-1.4882250749022212
1101	92-93	-1.5612595018911044
1101	94-95	-1.5535038576411395
1101	96-97	-1.6299655902475365
1101	98-99	-1.572458636911179
1101	100	-1.4095745356345617
1102	1	0.07099388876533652
1102	2	0.10187501012644873
1102	3	0.07611203908307118
1102	4	0.11965589229777862
1102	5	0.10148969072540126
1102	6	0.12477221390143711
1102	7	0.14199323219490623
1102	8	0.12265291722803795
1102	9	0.11890157625298059
1102	10-11	0.07524801954764371
1102	12-13	0.05094674947122968
1102	14-15	0.03511291365936131
1102	16-17	0.04819756697784072
1102	18-19	0.058716566121944425
1102	20-21	0.045804097071595606
1102	22-23	0.06975844930369135
1102	24-25	0.046178951621676845
1102	26-27	-0.010921291581446724
1102	28-29	0.013891426803091633
1102	30-31	-0.008799263924892387
1102	32-33	-0.058378384331206234
1102	34-35	-0.16011717813692883
1102	36-37	-0.08778435638235038
1102	38-39	-0.023721086841177907
1102	40-41	-0.11856801160057984
1102	42-43	-0.17055034842971395
1102	44-45	-0.2665770970082235
1102	46-47	-0.2899080323914518
1102	48-49	-0.38141078291880604
1102	50-51	-0.34441507340863353
1102	52-53	-0.4053832161857045
1102	54-55	-0.4312513713164421
1102	56-57	-0.4306525882577077
1102	58-59	-0.5210190672838806
1102	60-61	-0.5398999186900433
1102	62-63	-0.6131469125125335
1102	64-65	-0.6533683187668267
1102	66-67	-0.7368124014324309
1102	68-69	-0.7487491697399946
1102	70-71	-0.8916412365262119
1102	72-73	-0.8370662131170761
1102	74-75	-0.9974865017656782
1102	76-77	-0.880260962734603
1102	78-79	-0.900027897426142
1102	80-81	-0.9001014985673557
1102	82-83	-0.9805013372413072
1102	84-85	-1.0969555376280589
1102	86-87	-1.1604305286020136
1102	88-89	-1.1985120061578236
1102	90-91	-1.1973190727985283
1102	92-93	-1.2458663790560394
1102	94-95	-1.3443779238852613
1102	96-97	-1.4549344682656056
1102	98-99	-1.3368023301645842
1102	100	-1.156758548531716
1103	1	-0.02997275944528255
1103	2	0.033127520014652134
1103	3	0.03873987901922504
1103	4	0.0766961681382412
1103	5	0.10363912143430554
1103	6	0.0810877407699877
1103	7	0.07710763300251955
1103	8	0.06674354191293475
1103	9	0.09117479193583478
1103	10-11	0.0061118002435449625
1103	12-13	0.09742437674369597
1103	14-15	0.13957107321559192
1103	16-17	0.13922398598337793
1103	18-19	0.12909807665371176
1103	20-21	0.08024286814129766
1103	22-23	0.15274074067649224
1103	24-25	0.10360459523150212
1103	26-27	0.18002224011073764
1103	28-29	0.07869967903547348
1103	30-31	0.11386987511816926
1103	32-33	0.09664181482050083
1103	34-35	0.022137272150864362
1103	36-37	-0.03796815260800912
1103	38-39	1.0263938336407818E-4
1103	40-41	0.054869411852777716
1103	42-43	0.013576436944092052
1103	44-45	-0.11188733591078659
1103	46-47	-0.02999961305690846
1103	48-49	-0.1475001400883187
1103	50-51	-0.011372578934292221
1103	52-53	-0.09316240367763129
1103	54-55	-0.07394855362571917
1103	56-57	-0.19228786441976808
1103	58-59	-0.19853557036771008
1103	60-61	-0.28063444101380597
1103	62-63	-0.24689955945098774
1103	64-65	-0.2964190911711384
1103	66-67	-0.41880602961901303
1103	68-69	-0.3233012251384899
1103	70-71	-0.42200950847836083
1103	72-73	-0.3803152569759618
1103	74-75	-0.43720113745674993
1103	76-77	-0.4338102387221916
1103	78-79	-0.3685791925039297
1103	80-81	-0.4206410574814399
1103	82-83	-0.4378432440999731
1103	84-85	-0.5285370627694626
1103	86-87	-0.578058909844124
1103	88-89	-0.6340231948349135
1103	90-91	-0.6405803106079659
1103	92-93	-0.6757941111022845
1103	94-95	-0.7318225765248059
1103	96-97	-0.7238205223508487
1103	98-99	-0.6766154306257377
1103	100	-0.6309994990504073
1104	1	0.07348480191046391
1104	2	0.07453227907253179
1104	3	0.09195150117037798
1104	4	0.08881908314596387
1104	5	0.09415720854013898
1104	6	0.1108693876436746
1104	7	0.10116578588633018
1104	8	0.10615711754386581
1104	9	0.13265703814668228
1104	10-11	0.10653594612010409
1104	12-13	0.10440515314416388
1104	14-15	0.13577758051803102
1104	16-17	0.10738734706447417
1104	18-19	0.15445697160803462
1104	20-21	0.15654586038051832
1104	22-23	0.17964013856425254
1104	24-25	0.14765516481377716
1104	26-27	0.1628030619020322
1104	28-29	0.12994144561923093
1104	30-31	0.09922450106600422
1104	32-33	0.1159848682372484
1104	34-35	0.04026183474375955
1104	36-37	0.08128561310649474
1104	38-39	0.06121831559955382
1104	40-41	0.07622515243973282
1104	42-43	0.14185128089211219
1104	44-45	0.08488567877458308
1104	46-47	0.08496355055748239
1104	48-49	0.13119608178396902
1104	50-51	0.1121834505230268
1104	52-53	0.12488096860273146
1104	54-55	0.24327962248796808
1104	56-57	0.20582825985527364
1104	58-59	0.22042013794970927
1104	60-61	0.23069167286088188
1104	62-63	0.20192292067701345
1104	64-65	0.2349006096517705
1104	66-67	0.17122272648283143
1104	68-69	0.20524540638461986
1104	70-71	0.10654962302023563
1104	72-73	0.1361931803131391
1104	74-75	0.11129393961111589
1104	76-77	0.13129278310277925
1104	78-79	0.11753429194062903
1104	80-81	0.09440268736166502
1104	82-83	0.10068909029948614
1104	84-85	0.15364369889333318
1104	86-87	0.18007836011005196
1104	88-89	0.0713130047433772
1104	90-91	0.004921812123765079
1104	92-93	-0.061039777518661964
1104	94-95	-0.07332143164961735
1104	96-97	-0.01330919604793479
1104	98-99	0.053048507680017565
1104	100	0.05883694074507062
1105	1	0.06172949274699846
1105	2	0.058488417848550966
1105	3	0.03577706888982135
1105	4	0.04207248782272188
1105	5	0.0626110508435147
1105	6	0.052474013548952314
1105	7	0.06736000314869273
1105	8	0.0316693696688759
1105	9	0.047150334324520315
1105	10-11	0.08523868910032206
1105	12-13	0.07016379959576824
1105	14-15	0.09390421912963376
1105	16-17	0.11726584113111471
1105	18-19	0.13062411133329732
1105	20-21	-0.004849496961242039
1105	22-23	0.07388339264495158
1105	24-25	0.14785480504379933
1105	26-27	0.07862335554987254
1105	28-29	0.09031341604685394
1105	30-31	0.07874000196679276
1105	32-33	0.11911042285899498
1105	34-35	0.1401715851780594
1105	36-37	0.1401540769934968
1105	38-39	0.17728364237982674
1105	40-41	0.15027218319298186
1105	42-43	0.13823696476682557
1105	44-45	0.14173153398123617
1105	46-47	0.18895703587512713
1105	48-49	0.1851999546744878
1105	50-51	0.24023467170754031
1105	52-53	0.23736812265388352
1105	54-55	0.2903029288876908
1105	56-57	0.2810758054015068
1105	58-59	0.22182525652795704
1105	60-61	0.19871369880584666
1105	62-63	0.20451195143600387
1105	64-65	0.18777842461563665
1105	66-67	0.25296051740164316
1105	68-69	0.30233759132013205
1105	70-71	0.2715371529729822
1105	72-73	0.16716914053580112
1105	74-75	0.12526783301851907
1105	76-77	0.154168866436585
1105	78-79	0.20381019796075606
1105	80-81	0.18038072120039317
1105	82-83	0.24539230972100512
1105	84-85	0.20798968641924986
1105	86-87	0.16508499003960253
1105	88-89	0.0992933478334983
1105	90-91	0.12068044477848261
1105	92-93	0.14497414527325247
1105	94-95	0.18578908559520357
1105	96-97	0.17539526350604717
1105	98-99	0.22008315051077432
1105	100	0.22378935407334666
1106	1	-0.06575141432942289
1106	2	-0.03786993738140154
1106	3	-0.05642560724946577
1106	4	-0.040058426933335056
1106	5	-0.054081232878985475
1106	6	-0.049538710511143336
1106	7	-0.012488752811272263
1106	8	-0.03871096710641808
1106	9	-0.043508952017923264
1106	10-11	-0.03703858372846014
1106	12-13	-0.07830353906388154
1106	14-15	-0.09612277755142173
1106	16-17	-0.10624831982271132
1106	18-19	-0.05472732175210382
1106	20-21	-0.22485071034940063
1106	22-23	-0.08014282697012476
1106	24-25	-0.062263311915437214
1106	26-27	-0.08342236079354137
1106	28-29	-0.010235050795046163
1106	30-31	0.0033018450566970614
1106	32-33	0.02478200439207967
1106	34-35	-0.0397969722748428
1106	36-37	-0.05994071818292923
1106	38-39	-0.04543052028906658
1106	40-41	-0.03760819244034508
1106	42-43	0.0037115890999501744
1106	44-45	0.012376783649706624
1106	46-47	0.009995031777741303
1106	48-49	0.03643746697906636
1106	50-51	0.005685198388313495
1106	52-53	-0.004963207602038722
1106	54-55	0.07485184667789468
1106	56-57	0.09270524880417241
1106	58-59	0.10484984920724827
1106	60-61	0.16791976411488108
1106	62-63	0.15067589918079705
1106	64-65	0.17089140965683924
1106	66-67	0.15682465786809274
1106	68-69	0.19499252091834407
1106	70-71	0.16078776991721
1106	72-73	0.17509186901098417
1106	74-75	0.11763650507589318
1106	76-77	0.12318969865879126
1106	78-79	0.22451931976250705
1106	80-81	0.2056706137931812
1106	82-83	0.2605075700277517
1106	84-85	0.2685953457114145
1106	86-87	0.32538700818639654
1106	88-89	0.26487091712239774
1106	90-91	0.3126040998802857
1106	92-93	0.23865765041974996
1106	94-95	0.2722731646052807
1106	96-97	0.3335800650353704
1106	98-99	0.2891372363363054
1106	100	0.15330140094409828
1107	1	-0.21549585665430726
1107	2	-0.18008676729947126
1107	3	-0.15286916971706077
1107	4	-0.14699535572056988
1107	5	-0.1891795452993179
1107	6	-0.18462093597000262
1107	7	-0.3004554266179369
1107	8	-0.28414244790776877
1107	9	-0.3508461857821885
1107	10-11	-0.25520759083556754
1107	12-13	-0.21140799976123503
1107	14-15	-0.1875535062133551
1107	16-17	-0.22842777530654246
1107	18-19	-0.23173464188332815
1107	20-21	-0.4315233529221345
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2207	86-87	0.5586906188423697
2207	88-89	0.5834627318907906
2207	90-91	0.5597143499628174
2207	92-93	0.7258819351735539
2207	94-95	0.5991293146880317
2207	96-97	0.734015540820053
2207	98-99	0.8790277639614175
2207	100	0.8297130539190718
2208	1	-0.28891810663917283
2208	2	-0.2527791871330578
2208	3	-0.234715931319883
2208	4	-0.250422153372881
2208	5	-0.27133581115094074
2208	6	-0.45081778558927965
2208	7	-0.6462459186772236
2208	8	-0.40758696342703615
2208	9	-0.41091842087530495
2208	10-11	-0.4721300541590807
2208	12-13	-0.38795632113032497
2208	14-15	-0.3818583819012815
2208	16-17	-0.4121297358500442
2208	18-19	-0.4607484342420278
2208	20-21	-0.33845894241362373
2208	22-23	-0.45819774869413266
2208	24-25	-0.56386134836373
2208	26-27	-0.4128455226611649
2208	28-29	-0.527195362572165
2208	30-31	-0.27614443516934273
2208	32-33	-0.4277349452754464
2208	34-35	-0.37789904756871806
2208	36-37	-0.25309257230995996
2208	38-39	-0.6105751956426815
2208	40-41	-0.32721332217886356
2208	42-43	-0.539436988671973
2208	44-45	-0.35954565821288753
2208	46-47	-0.5476305740428487
2208	48-49	-0.22203574135979665
2208	50-51	-0.04746741007296151
2208	52-53	0.009864092371934419
2208	54-55	-0.9199359834917118
2208	56-57	-0.424018050241763
2208	58-59	-0.31224709227072367
2208	60-61	0.004668179634148117
2208	62-63	0.17477467258330392
2208	64-65	0.02587687276548678
2208	66-67	0.4401382868413677
2208	68-69	0.4868958759594726
2208	70-71	0.11105629348765689
2208	72-73	0.39408814946084547
2208	74-75	0.6813246148147982
2208	76-77	0.6639595476198537
2208	78-79	0.5804259272138808
2208	80-81	0.7822044617004877
2208	82-83	0.687920950021514
2208	84-85	0.6476717889153498
2208	86-87	0.7785775985268941
2208	88-89	1.29685663969342
2208	90-91	1.367149450929979
2208	92-93	1.6553049471319206
2208	94-95	1.7732571387712213
2208	96-97	1.995870146767757
2208	98-99	2.166267018424552
2208	100	1.9187017086545985
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	357455.0
3	121271.0
4	81063.0
5	68623.0
6	71219.0
7	73774.0
8	80670.0
9	92254.0
10	105191.0
11	117893.0
12	137176.0
13	182118.0
14	234259.0
15	261403.0
16	315988.0
17	323265.0
18	296191.0
19	263656.0
20	264516.0
21	278436.0
22	297366.0
23	321204.0
24	351726.0
25	386065.0
26	429518.0
27	484985.0
28	552671.0
29	632548.0
30	729600.0
31	850700.0
32	1006146.0
33	1225942.0
34	1557483.0
35	2077350.0
36	2951881.0
37	4264146.0
38	4026761.0
39	802959.0
40	1224.0
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	21.175838267227697	28.19322902656311	28.61761066662828	22.013322039580913
2	23.13717498740706	27.769186644613896	27.631703096438066	21.461935271540977
3	23.066904463969994	26.634257618718436	27.34628878052635	22.95254913678522
4	23.801390044318516	26.308420337933924	27.227944056346715	22.662245561400844
5	23.98049968406882	26.6664694908245	26.810224174687903	22.54280665041878
6	24.068231866280478	26.556477123707655	26.270974277151748	23.10431673286012
7	24.004148048426288	26.95416127583191	26.485772038700272	22.55591863704153
8	23.653298353227463	26.731085787300007	26.68652086848768	22.929094990984854
9	23.4126053071086	26.48747802301915	26.86547796948031	23.234438700391934
10-11	23.27556583959478	26.701595735587723	26.841156805955563	23.18168161886193
12-13	23.01899655511239	26.738039334475754	27.02586121252996	23.217102897881894
14-15	22.795550119489462	26.92654650757882	27.399338298435044	22.878565074496677
16-17	22.687351394837947	26.833690009779588	27.579980196382408	22.89897839900006
18-19	22.331434761460212	26.835089342803414	28.192778990092	22.640696905644376
20-21	22.0502282580297	26.56569985072491	28.79244985129199	22.5916220399534
22-23	22.0988325508971	26.520953306160216	29.110129852795	22.270084290147675
24-25	22.013569111812554	26.426527755679658	29.479823471307075	22.080079661200713
26-27	22.080322817859354	26.471400001462104	29.461499111583567	21.986778069094974
28-29	22.092490042460806	26.525627212109498	29.445004327669444	21.93687841776026
30-31	22.091414966307006	26.480200056626945	29.40674979463218	22.021635182433865
32-33	22.088637868243755	26.535094905194534	29.32190608360979	22.054361142951922
34-35	22.151411978393604	26.599178970130037	29.3172433450936	21.932165706382754
36-37	22.172658043169072	26.57930886069183	29.199177877773387	22.048855218365706
38-39	22.193341248091546	26.639133457392756	29.09057817669979	22.076947117815912
40-41	22.270661176107605	26.756738967881216	28.91494040817951	22.057659447831664
42-43	22.371688426383475	26.819060870083426	28.550475205337534	22.25877549819556
44-45	22.42337925922567	26.990157070860494	28.21882654583246	22.36763712408138
46-47	22.647715767679482	27.147878674485664	27.806190413469384	22.398215144365466
48-49	22.727036160666483	27.138037465050868	27.51032295651345	22.624603417769194
50-51	22.711599101611245	27.256048855371674	27.345044155533504	22.687307887483577
52-53	22.808975200557537	27.308740705973033	27.225940312037128	22.6563437814323
54-55	22.859881249254823	27.24800059488432	27.146359523761802	22.745758632099054
56-57	22.84710843836902	27.295488224957015	27.12731123342752	22.730092103246445
58-59	22.920719209450734	27.3724156430056	27.0741914908221	22.63267365672157
60-61	22.904135416042003	27.335829677899344	27.022828387649135	22.737206518409522
62-63	22.926751541127615	27.391281149004786	27.003062813377987	22.67890449648961
64-65	22.979375265210138	27.446912419748976	26.948932234653206	22.62478008038768
66-67	22.99445326500353	27.445704818912333	26.872645937134255	22.68719597894989
68-69	22.99467494003368	27.546073994874664	26.77171203531783	22.68753902977383
70-71	23.07888384760043	27.61135898072617	26.71015428584636	22.599602885827043
72-73	23.081655011523054	27.635991744294625	26.590297930371452	22.692055313810865
74-75	23.126845085563534	27.71498237876858	26.543539679740967	22.614632855926924
76-77	22.977139776986018	27.902259355890696	26.407348695865533	22.71325217125775
78-79	23.026345996744624	27.91553782790007	26.31895204790599	22.73916412744931
80-81	23.0494991139885	28.00573501577358	26.211184487925017	22.733581382312902
82-83	23.07735915398696	28.095923635245924	26.124509501989436	22.702207708777678
84-85	23.06571300033145	28.116898528729934	26.022228232172985	22.795160238765632
86-87	23.09622145305058	28.225142265178665	25.899524425581895	22.779111856188862
88-89	23.226780390982693	28.29432307237093	25.727747289338836	22.751149247307538
90-91	23.202406085465704	28.286405317559705	25.627994758878476	22.88319383809612
92-93	23.225196973200436	28.372908244947954	25.544204281320688	22.857690500530925
94-95	23.294280491706505	28.447286274129326	25.39774260499419	22.86069062916998
96-97	23.262045271388505	28.513450987672844	25.228682582107542	22.99582115883111
98-99	23.285835041496384	28.55834937278382	25.11999878237384	23.035816803345952
100	23.927102101595484	28.40343150376284	24.324361307369493	23.345105087272184
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	4739.0
1	3237.5
2	1579.5
3	1462.0
4	1640.5
5	1918.5
6	2160.5
7	2445.5
8	2753.5
9	3132.5
10	3764.0
11	4656.5
12	5634.0
13	7479.0
14	12771.0
15	17385.5
16	23596.5
17	25604.0
18	23913.0
19	28597.5
20	41293.0
21	51837.5
22	59677.0
23	74802.0
24	92880.0
25	114907.0
26	141147.5
27	171923.0
28	207392.5
29	245456.0
30	285197.5
31	327410.0
32	376833.5
33	432314.0
34	491877.0
35	557265.5
36	630744.5
37	716476.0
38	793229.0
39	839413.0
40	870106.5
41	920274.0
42	1008074.5
43	1088804.0
44	1151958.0
45	1191206.0
46	1170372.0
47	1110365.0
48	1051551.0
49	1022548.0
50	991954.0
51	935558.0
52	855051.5
53	759864.5
54	689302.5
55	638713.0
56	577296.0
57	512144.5
58	461438.0
59	417864.5
60	369676.5
61	324320.0
62	284323.0
63	245803.0
64	211837.5
65	181381.0
66	153032.5
67	127828.0
68	106271.5
69	87389.5
70	70862.0
71	57637.0
72	46497.0
73	37585.5
74	30505.5
75	23696.5
76	17912.0
77	13895.5
78	10953.0
79	8099.0
80	5422.5
81	3525.5
82	2231.5
83	1347.5
84	798.0
85	488.5
86	297.0
87	180.0
88	116.0
89	80.0
90	39.0
91	16.0
92	10.0
93	8.0
94	5.0
95	3.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.04108454810145904
3	7.309750802723096E-4
4	0.0026689961905327405
5	0.0
6	0.008831678405751598
7	0.0038198133681922233
8	0.01217916941438325
9	0.01675619799393448
10-11	0.008816684045130627
12-13	0.008944136110408875
14-15	0.009695728436535019
16-17	0.01610581760199989
18-19	0.02657188131543726
20-21	0.017983861269776434
22-23	0.021464427228919204
24-25	0.01024114830412282
26-27	0.010612258729491838
28-29	0.02418590368162534
30-31	0.021170162901732657
32-33	0.015286750653079377
34-35	0.27469293798602346
36-37	0.34518705015043843
38-39	0.37554875611282595
40-41	0.3604663036232073
42-43	0.3633246036165798
44-45	0.38439355458412094
46-47	0.40418985919395717
48-49	0.20739262463387523
50-51	0.016872404288747003
52-53	0.008644248897989466
54-55	0.034961226082870236
56-57	0.05475378210255123
58-59	0.0299793497665528
60-61	0.014853788490148855
62-63	0.027833281902676407
64-65	0.016681226190829627
66-67	0.022682719029373053
68-69	0.008803563979587276
70-71	0.013159425739979193
72-73	0.010417332041419224
74-75	0.01302635078946808
76-77	0.02414092059976243
78-79	0.029544513308544657
80-81	0.009982495583411079
82-83	0.0199949798880641
84-85	0.015985862717032125
86-87	0.012250392627332858
88-89	0.004472068055204437
90-91	0.015596009340886893
92-93	0.01487628003108031
94-95	0.009034102274134698
96-97	0.004642628907267977
98-99	0.006904903065956894
100	0.010031227255429232
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	2.6676696E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	47.535147897622075
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.59717360881639	38.311985676254096
2	10.935512138163087	10.396423736476477
3	3.4631531313484474	4.938644888722844
4	1.5050561072401354	2.861722586075168
5	0.7906731918624226	1.8792383556932586
6	0.48724120941162535	1.3896649770718714
7	0.3410028923057126	1.134673604348826
8	0.22683066458750273	0.8625943351106278
9	0.18228873981871532	0.7798609986618404
>10	1.2102003748265004	11.129798441941238
>50	0.08431978508488748	2.881367596510504
>100	0.15613209807354142	15.977518541206665
>500	0.019154988002666296	5.975822647592586
>1k	0.0011271122516442124	0.6864524862344559
>5k	4.727936709214198E-5	0.14764128028326542
>10k+	8.667883965907617E-5	0.6465898478163691
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	31537	0.11821928772588629	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	7.497180310485226E-6	0.0
2	0.0	3.748590155242613E-6	0.0	1.8742950776213068E-5	0.0
3	0.0	7.497180310485226E-6	0.0	1.8742950776213068E-5	0.0
4	0.0	7.497180310485226E-6	0.0	2.249154093145568E-5	0.0
5	0.0	7.497180310485226E-6	0.0	2.6240131086698292E-5	0.0
6	0.0	7.497180310485226E-6	0.0	2.6240131086698292E-5	0.0
7	0.0	7.497180310485226E-6	0.0	2.6240131086698292E-5	0.0
8	0.0	7.497180310485226E-6	0.0	2.9988721241940905E-5	0.0
9	3.748590155242613E-6	7.497180310485226E-6	0.0	3.373731139718352E-5	0.0
10-11	2.249154093145568E-5	9.371475388106534E-6	0.0	3.373731139718352E-5	0.0
12-13	1.1245770465727841E-4	1.686865569859176E-5	0.0	3.373731139718352E-5	0.0
14-15	4.0484773676620226E-4	1.8742950776213068E-5	0.0	3.373731139718352E-5	3.748590155242613E-6
16-17	9.015359323358485E-4	1.8742950776213068E-5	0.0	4.123449170766875E-5	3.748590155242613E-6
18-19	0.0018049461597493184	2.4365836009076986E-5	0.0	4.685737694053266E-5	3.748590155242613E-6
20-21	0.0030288608454360315	2.9988721241940905E-5	0.0	4.873167201815397E-5	3.748590155242613E-6
22-23	0.00457702857955123	3.5611606474804826E-5	0.0	4.873167201815397E-5	3.748590155242613E-6
24-25	0.006488809558724964	4.310878678529006E-5	0.0	5.2480262173396585E-5	3.748590155242613E-6
26-27	0.008936638930098391	4.498308186291136E-5	0.0	5.62288523286392E-5	5.62288523286392E-6
28-29	0.011305747908211723	4.873167201815397E-5	0.0	5.997744248388181E-5	7.497180310485226E-6
30-31	0.013067585281175749	5.62288523286392E-5	0.0	5.997744248388181E-5	7.497180310485226E-6
32-33	0.014756325146112546	5.997744248388181E-5	0.0	5.997744248388181E-5	7.497180310485226E-6
34-35	0.01648067661752415	6.560032771674573E-5	0.0	6.747462279436703E-5	7.497180310485226E-6
36-37	0.018499292416122297	7.122321294960965E-5	0.0	7.497180310485227E-5	7.497180310485226E-6
38-39	0.02048229660824564	7.309750802723096E-5	0.0	8.059468833771619E-5	7.497180310485226E-6
40-41	0.0223790832267984	7.497180310485227E-5	0.0	8.62175735705801E-5	1.124577046572784E-5
42-43	0.024615117254400617	7.497180310485227E-5	0.0	8.62175735705801E-5	1.124577046572784E-5
44-45	0.027411565510211608	7.497180310485227E-5	0.0	8.62175735705801E-5	1.124577046572784E-5
46-47	0.03112829264913466	7.497180310485227E-5	0.0	9.558904895868664E-5	1.124577046572784E-5
48-49	0.0355834920486405	7.497180310485227E-5	0.0	1.0870911450203577E-4	1.124577046572784E-5
50-51	0.0407996552496606	7.497180310485227E-5	0.0	1.124577046572784E-4	1.124577046572784E-5
52-53	0.04732220211978275	7.872039326009488E-5	0.0	1.1995488496776362E-4	1.124577046572784E-5
54-55	0.055898976394977845	7.872039326009488E-5	0.0	1.2182918004538493E-4	1.124577046572784E-5
56-57	0.06640252600996766	8.43432784929588E-5	0.0	1.2557777020062754E-4	1.124577046572784E-5
58-59	0.0795507059794811	9.184045880344402E-5	1.8742950776213065E-6	1.3120065543349146E-4	1.124577046572784E-5
60-61	0.09586831892525222	1.0683481942441449E-4	3.748590155242613E-6	1.386978357439767E-4	1.124577046572784E-5
62-63	0.11570210943664089	1.0870911450203579E-4	3.748590155242613E-6	1.461950160544619E-4	1.4994360620970452E-5
64-65	0.14038282701875823	1.124577046572784E-4	3.748590155242613E-6	1.5181790128732586E-4	1.4994360620970452E-5
66-67	0.17111939199667003	1.1620629481252101E-4	3.748590155242613E-6	1.5744078652018975E-4	1.4994360620970452E-5
68-69	0.20820419440248522	1.1620629481252101E-4	3.748590155242613E-6	1.6681226190829628E-4	1.4994360620970452E-5
70-71	0.2532341336423371	1.2370347512300625E-4	5.62288523286392E-6	1.7243514714116023E-4	1.4994360620970452E-5
72-73	0.3066290518136129	1.2370347512300625E-4	7.497180310485226E-6	1.8368091760688805E-4	1.4994360620970452E-5
74-75	0.37022950668253674	1.2557777020062754E-4	7.497180310485226E-6	1.8930380283975197E-4	1.4994360620970452E-5
76-77	0.44567550644202714	1.3307495051111275E-4	7.497180310485226E-6	2.0242386838310113E-4	1.4994360620970452E-5
78-79	0.5339473074176802	1.3682354066635538E-4	7.497180310485226E-6	2.0992104869358634E-4	1.4994360620970452E-5
80-81	0.6343795348569403	1.4057213082159802E-4	7.497180310485226E-6	2.1554393392645026E-4	1.4994360620970452E-5
82-83	0.7500197925560197	1.461950160544619E-4	7.497180310485226E-6	2.2116681915931418E-4	1.686865569859176E-5
84-85	0.8818014794635738	1.461950160544619E-4	7.497180310485226E-6	2.249154093145568E-4	1.8742950776213068E-5
86-87	1.0315107987885757	1.461950160544619E-4	7.497180310485226E-6	2.249154093145568E-4	1.8742950776213068E-5
88	1.155337977386705	1.4994360620970454E-4	7.497180310485226E-6	2.2866399946979942E-4	2.249154093145568E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATCTCGA	18015	0.0	51.070858	1
TCTCGAG	22280	0.0	41.3379	2
CTCGAGG	28145	0.0	32.59845	3
TCGAGGG	32075	0.0	28.399368	4
GATCCGG	23300	0.0	23.34133	1
CGAGGGC	45045	0.0	20.347492	5
ATCCGGC	27200	0.0	19.85706	2
AGGGCGC	46180	0.0	19.6751	7
TATCTCG	9420	0.0	19.24462	1
GGGCGCG	49585	0.0	18.38083	8
GAGGGCG	52545	0.0	17.38097	6
GGATCCG	16585	0.0	16.16939	1
TCCGGCG	35160	0.0	15.584599	3
GCGCCGT	35455	0.0	15.256106	9
CGGCGCG	36880	0.0	14.743321	5
GGCGCGC	83850	0.0	13.685697	9
ATGGATC	18230	0.0	13.344922	1
CCGGCGC	42165	0.0	13.0066185	4
CGTCGAC	34050	0.0	12.557878	12-13
ATATCTC	13765	0.0	12.453448	1
>>END_MODULE