FastQCFastQC Report
Wed 25 May 2016
SRR491039_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR491039_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27315084
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCTCGA182400.047.789181
TCTCGAG219700.039.4633522
CTCGAGG274350.031.4060213
TCGAGGG317600.026.9962144
GATCCGG238950.021.7775571
CGAGGGC437650.019.6769315
ATCCGGC275050.018.8175162
TATCTCG104350.018.408031
AGGGCGC468650.018.2356517
GGGCGCG512500.016.7762078
GAGGGCG525950.016.4183776
TCCGGCG349750.015.0497653
GCGCCGT353500.014.5707129
CGGCGCG370150.013.9667235
GGATCCG179200.013.4186371
CGTCGAC335350.012.99570612-13
GGCGCGC845100.012.9174989
CCGGCGC415000.012.5930144
ATGGATC202500.012.4544831
TCGACTT357200.012.25985114-15
Produced by FastQC (version 0.11.5)