FastQCFastQC Report
Wed 25 May 2016
SRR491038_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR491038_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47512972
Sequences flagged as poor quality0
Sequence length100
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCTCGA349950.045.382581
TCTCGAG421750.037.309452
TGATACG53150.035.459183
ATACGGC60050.031.3065365
TATCTCG163900.029.7937452
CTCGAGG525650.029.5592733
TCGAGGG595300.025.9113624
ATATCTC209650.023.9551431
GATCCGG501600.023.2369211
ATCCGGC577550.020.1488172
GATACGG95700.019.791594
ACGACGC47500.019.09558540-41
TACGGCG98650.018.6280826
AGGGCGC884800.017.4605777
CGAGGGC899000.017.2155175
TATGGAT319900.016.4254042
GGGCGCG945650.016.3966798
TCCGGCG721600.016.1656363
ATGATAC124350.015.7986132
GGATCCG352450.015.3485122
Produced by FastQC (version 0.11.5)