FastQCFastQC Report
Wed 25 May 2016
SRR491037_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR491037_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39391742
Sequences flagged as poor quality0
Sequence length100
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA463280.11760840635075241No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCTCGA292250.044.0077551
TCTCGAG341750.037.406262
CTCGAGG438050.029.0217553
TCGAGGG491250.025.6683654
TATCTCG152500.023.637932
GATCCGG420900.022.8222961
TGATACG42400.020.9496153
ATATCTC179300.020.7046071
ATCCGGC476200.020.2817942
TCCGGCG540450.017.8096143
TATGGAT211550.017.4841962
CGAGGGC724850.017.4350345
AGGGCGC720300.017.4019537
ATACGGC53800.016.5104775
GGGCGCG782200.016.0248368
GCGCCGT598750.015.8482129
CGGCGCG622450.015.3426125
GAGGGCG827600.015.3101536
ATATGGA257450.014.968611
GGATCCG295550.014.3595482
Produced by FastQC (version 0.11.5)