##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR491037_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	39391742
Sequences flagged as poor quality	0
Sequence length	100
%GC	47
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.21413241892171	31.0	30.0	34.0	26.0	34.0
2	30.525528345509574	31.0	31.0	34.0	27.0	34.0
3	30.63266250068352	31.0	31.0	34.0	27.0	34.0
4	34.0963891873581	37.0	35.0	37.0	30.0	37.0
5	33.69045864993734	37.0	35.0	37.0	28.0	37.0
6	33.5900855565108	37.0	35.0	37.0	28.0	37.0
7	33.50638583081703	37.0	35.0	37.0	28.0	37.0
8	33.45789746998241	37.0	35.0	37.0	28.0	37.0
9	34.865553622888775	39.0	35.0	39.0	27.0	39.0
10-11	34.785396670195496	39.0	35.0	39.0	27.0	39.0
12-13	34.721369126046774	39.0	35.0	39.0	27.0	39.0
14-15	35.78789405911523	40.0	36.0	41.0	27.0	41.0
16-17	35.71435358202742	39.0	36.0	41.0	27.0	41.0
18-19	35.66645713459435	39.5	35.5	41.0	27.0	41.0
20-21	35.602653685637975	39.0	35.0	41.0	27.0	41.0
22-23	35.47154347731055	39.0	35.0	41.0	26.0	41.0
24-25	35.337892558800775	39.0	35.0	41.0	25.0	41.0
26-27	35.161616018403045	39.0	35.0	41.0	25.0	41.0
28-29	34.928237014752995	39.0	34.0	41.0	24.0	41.0
30-31	34.73558834996432	39.0	34.0	41.0	24.0	41.0
32-33	34.49572590620643	38.0	34.0	41.0	23.0	41.0
34-35	34.32693938744826	38.0	34.0	40.0	21.5	41.0
36-37	34.12850513186241	38.0	33.0	40.0	20.5	41.0
38-39	33.9155972716312	38.0	33.0	40.0	18.5	41.0
40-41	33.64515513175325	38.0	33.0	40.0	16.0	41.0
42-43	33.297325337884274	38.0	33.0	40.0	13.5	41.0
44-45	32.95189605983914	38.0	32.0	40.0	8.5	41.0
46-47	32.79541017759509	38.0	32.0	40.0	7.5	41.0
48-49	32.60655228194783	38.0	32.0	40.0	2.0	41.0
50-51	32.342735883576815	37.5	31.0	40.0	2.0	41.0
52-53	32.08872181890305	37.0	31.0	40.0	2.0	41.0
54-55	31.815205684480773	37.0	31.0	40.0	2.0	41.0
56-57	31.50690807225535	36.0	30.0	40.0	2.0	41.0
58-59	31.189725323647785	36.0	30.0	40.0	2.0	41.0
60-61	30.86976493702665	35.0	29.5	39.0	2.0	41.0
62-63	30.60694693319224	35.0	29.0	39.0	2.0	41.0
64-65	30.482818010434777	35.0	29.0	39.0	2.0	41.0
66-67	30.258638054138352	35.0	29.0	38.5	2.0	40.5
68-69	29.937681760811696	35.0	29.0	38.0	2.0	40.0
70-71	29.577805939630696	35.0	29.0	37.0	2.0	40.0
72-73	29.204398399035007	35.0	29.0	36.5	2.0	39.0
74-75	28.85391906506699	34.0	28.0	36.0	2.0	39.0
76-77	28.056193059448855	33.5	26.5	35.0	2.0	38.0
78-79	28.212372240354334	34.0	27.0	35.0	2.0	37.0
80-81	27.952482502550914	34.0	27.0	35.0	2.0	37.0
82-83	27.72335991132355	34.0	27.0	35.0	2.0	36.5
84-85	27.457184769843387	34.0	26.0	35.0	2.0	36.0
86-87	27.16696010803483	34.0	26.0	35.0	2.0	36.0
88-89	26.930133820941457	34.0	25.5	35.0	2.0	35.5
90-91	26.621272346879202	34.0	25.0	35.0	2.0	35.0
92-93	26.406286246492982	34.0	24.5	35.0	2.0	35.0
94-95	26.03247523300696	33.0	23.5	35.0	2.0	35.0
96-97	25.78786457323974	33.0	21.5	35.0	2.0	35.0
98-99	25.43368566944818	33.0	19.0	35.0	2.0	35.0
100	24.655445727685766	32.0	2.0	35.0	2.0	35.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	0.711521138849271
1101	2	0.6900184217929457
1101	3	0.6993561742161418
1101	4	0.777069598847099
1101	5	0.9570484146602212
1101	6	0.9970330784111354
1101	7	0.9208321214283828
1101	8	1.0136659035272189
1101	9	1.032554978582823
1101	10-11	1.1140185074680389
1101	12-13	1.1205944103552525
1101	14-15	1.2127424117539007
1101	16-17	1.2157083008847707
1101	18-19	1.1594154438919944
1101	20-21	1.2398076438512433
1101	22-23	1.264091548461387
1101	24-25	1.320914787974175
1101	26-27	1.3692970075259154
1101	28-29	1.389186928998953
1101	30-31	1.4544378775295357
1101	32-33	1.3788019399464204
1101	34-35	1.4081632964995947
1101	36-37	1.3298116549952539
1101	38-39	1.3734192359885569
1101	40-41	1.3665586004296983
1101	42-43	1.3025857495326818
1101	44-45	1.3807597559195628
1101	46-47	1.168227387402375
1101	48-49	1.248162522476278
1101	50-51	1.172284347719014
1101	52-53	1.2753771168086558
1101	54-55	1.166393273156011
1101	56-57	1.1999454027536238
1101	58-59	1.0992256254495878
1101	60-61	1.139086249508626
1101	62-63	1.1920149140178289
1101	64-65	1.342125826622187
1101	66-67	1.2201420350046526
1101	68-69	1.1805436168976229
1101	70-71	1.1489488746541596
1101	72-73	1.18631486231617
1101	74-75	1.1713725010666352
1101	76-77	1.0653022176322828
1101	78-79	1.176745684304347
1101	80-81	1.1767361004037689
1101	82-83	1.1788327389111295
1101	84-85	1.1135012634671
1101	86-87	1.0367119211792222
1101	88-89	1.06354899283005
1101	90-91	1.1438629668101434
1101	92-93	1.1464223528959785
1101	94-95	1.2349794976202446
1101	96-97	1.1373287519118414
1101	98-99	1.0804138438746023
1101	100	1.081740885547525
1102	1	0.4188641681888363
1102	2	0.458400021265593
1102	3	0.45599473697126314
1102	4	0.4971624598526887
1102	5	0.497697197344813
1102	6	0.6299271792428769
1102	7	0.6355913899314487
1102	8	0.6861229851937267
1102	9	0.6024242150892718
1102	10-11	0.7480251921790284
1102	12-13	0.795959141999063
1102	14-15	0.827502346902925
1102	16-17	0.6389100929623908
1102	18-19	0.7497452380012462
1102	20-21	0.7475678999467803
1102	22-23	0.7575884468111767
1102	24-25	0.7880235019552515
1102	26-27	0.7532331582023915
1102	28-29	0.7648910193829437
1102	30-31	0.7442517731858445
1102	32-33	0.7291169002176545
1102	34-35	0.7473931827637372
1102	36-37	0.7770823344354767
1102	38-39	0.775490234008835
1102	40-41	0.7780301316755924
1102	42-43	0.7525268609991471
1102	44-45	0.7267972384128782
1102	46-47	0.7653342643564827
1102	48-49	0.7433499124458081
1102	50-51	0.6592027841851476
1102	52-53	0.6377186315192915
1102	54-55	0.5830563104552979
1102	56-57	0.7214939548845258
1102	58-59	0.7345151371605958
1102	60-61	0.622451514055701
1102	62-63	0.6117471037110072
1102	64-65	0.7343229530285882
1102	66-67	0.7891010936649501
1102	68-69	0.8050252557183981
1102	70-71	0.7849692811517563
1102	72-73	0.7186656993823775
1102	74-75	0.7846579511719938
1102	76-77	0.7319657313360999
1102	78-79	0.6236720534515889
1102	80-81	0.7930703865619684
1102	82-83	0.751936097269752
1102	84-85	0.7213767465145402
1102	86-87	0.6927527137666587
1102	88-89	0.5632050661382877
1102	90-91	0.5566624404901255
1102	92-93	0.6415868617786309
1102	94-95	0.8076423304464058
1102	96-97	0.6728336260649854
1102	98-99	0.5670451367756506
1102	100	0.5360831286254708
1103	1	0.14136602767568718
1103	2	0.10239420612622752
1103	3	0.12634936401574137
1103	4	0.19766187283488534
1103	5	0.28285604996621316
1103	6	0.21092869120135305
1103	7	0.20265080428126936
1103	8	0.29789754518363765
1103	9	0.3791014811861828
1103	10-11	0.3473128833180965
1103	12-13	0.2809564971604672
1103	14-15	0.2775581666412066
1103	16-17	0.2512014902266202
1103	18-19	0.3342326198981098
1103	20-21	0.33791618642060683
1103	22-23	0.2790355528807211
1103	24-25	0.2909216958300007
1103	26-27	0.22082978140762322
1103	28-29	0.3446484947177524
1103	30-31	0.33245718483463094
1103	32-33	0.3060751807051787
1103	34-35	0.32246383160465797
1103	36-37	0.35972965479462005
1103	38-39	0.3811883134172831
1103	40-41	0.34032721310742886
1103	42-43	0.3205302166463184
1103	44-45	0.4114185933955099
1103	46-47	0.296602322968198
1103	48-49	0.3460706489471548
1103	50-51	0.340866696445417
1103	52-53	0.3128510383028029
1103	54-55	0.19068373487652224
1103	56-57	0.2264334533039687
1103	58-59	0.33373229836953655
1103	60-61	0.2503942840353979
1103	62-63	0.4169473708000915
1103	64-65	0.44427832611629725
1103	66-67	0.3227486923988998
1103	68-69	0.3287399887071416
1103	70-71	0.33165324015044817
1103	72-73	0.31182955217180464
1103	74-75	0.42740025824587136
1103	76-77	0.417336087267298
1103	78-79	0.3682226593684881
1103	80-81	0.4339091113416025
1103	82-83	0.4372790805537967
1103	84-85	0.34785084463948124
1103	86-87	0.4559460401407769
1103	88-89	0.5047550114362345
1103	90-91	0.34406593897541526
1103	92-93	0.407795916468082
1103	94-95	0.5673944710877912
1103	96-97	0.4660140696127506
1103	98-99	0.39859813035953096
1103	100	0.4512553259721699
1104	1	-0.1378269825124221
1104	2	-0.10480570612742923
1104	3	-0.0621129898279662
1104	4	-0.041997355678439874
1104	5	-0.013539392628636904
1104	6	-0.06948995072043118
1104	7	-0.02666438594882692
1104	8	-0.08209157499567965
1104	9	-0.16072541450346733
1104	10-11	-0.21776772863489668
1104	12-13	-0.13686705506933805
1104	14-15	-0.16985332649323226
1104	16-17	-0.191652955903848
1104	18-19	-0.2751482890242656
1104	20-21	-0.22522622495721834
1104	22-23	-0.22007393503661632
1104	24-25	-0.20388864617787306
1104	26-27	-0.15200617409908546
1104	28-29	-0.35791909539548783
1104	30-31	-0.21428879058306904
1104	32-33	-0.348805758534553
1104	34-35	-0.21475153787169887
1104	36-37	-0.3784440280626171
1104	38-39	-0.3962882681860833
1104	40-41	-0.3032239759472901
1104	42-43	-0.24685197378573775
1104	44-45	-0.38936362021693327
1104	46-47	-0.30242811124884383
1104	48-49	-0.299407209723789
1104	50-51	-0.27450019608738074
1104	52-53	-0.2723591992119978
1104	54-55	-0.2677971641917942
1104	56-57	-0.29305493618643297
1104	58-59	-0.440749264719134
1104	60-61	-0.4198796247323955
1104	62-63	-0.20125926561289376
1104	64-65	-0.1960643365957111
1104	66-67	-0.3015581128065925
1104	68-69	-0.3160403950179962
1104	70-71	-0.3370484973263004
1104	72-73	-0.26886428820833785
1104	74-75	-0.3255718926929383
1104	76-77	-0.30664940767477233
1104	78-79	-0.3139332917982891
1104	80-81	-0.28793493367881595
1104	82-83	-0.2696311001613161
1104	84-85	-0.2664932305502212
1104	86-87	-0.23054096604174035
1104	88-89	-0.17626489966099612
1104	90-91	-0.18001966153323323
1104	92-93	-0.15983360192799623
1104	94-95	-0.011382958157650336
1104	96-97	-0.10125079840200257
1104	98-99	-0.11869620267943759
1104	100	-0.07809266733742959
1105	1	-0.4947941974548442
1105	2	-0.46497521907802764
1105	3	-0.40657765785940825
1105	4	-0.36690304847198263
1105	5	-0.5457765271364678
1105	6	-0.603904298418108
1105	7	-0.614067027390945
1105	8	-0.6224343142756581
1105	9	-0.6154331284831684
1105	10-11	-0.570729542001331
1105	12-13	-0.6056157125009634
1105	14-15	-0.734398738203673
1105	16-17	-0.6045979188107964
1105	18-19	-0.6534225802095293
1105	20-21	-0.6925784352223516
1105	22-23	-0.7119930980993914
1105	24-25	-0.570903743643548
1105	26-27	-0.55573467940944
1105	28-29	-0.6007218110860819
1105	30-31	-0.6335645324177719
1105	32-33	-0.6864650731460458
1105	34-35	-0.6362631326060466
1105	36-37	-0.6273341128576035
1105	38-39	-0.5803418513208669
1105	40-41	-0.5539006208550035
1105	42-43	-0.645100329283629
1105	44-45	-0.5224546635385181
1105	46-47	-0.674098601381516
1105	48-49	-0.6500299305923818
1105	50-51	-0.5254632816416347
1105	52-53	-0.5587285926381398
1105	54-55	-0.5400337960974113
1105	56-57	-0.4901564593406569
1105	58-59	-0.5722515617542356
1105	60-61	-0.5248619697703667
1105	62-63	-0.5543292591336133
1105	64-65	-0.6814289096004096
1105	66-67	-0.6718909315597443
1105	68-69	-0.7282697066165937
1105	70-71	-0.551341928619788
1105	72-73	-0.5876466851380222
1105	74-75	-0.5784976711543663
1105	76-77	-0.5497263242288071
1105	78-79	-0.5099465169185358
1105	80-81	-0.44090992003122764
1105	82-83	-0.424646473515093
1105	84-85	-0.533044486789322
1105	86-87	-0.41017314776371805
1105	88-89	-0.41836810589879647
1105	90-91	-0.44471144775212323
1105	92-93	-0.38722605410282185
1105	94-95	-0.2883333371554784
1105	96-97	-0.43193453481611854
1105	98-99	-0.4112321239242007
1105	100	-0.3026829707382319
1106	1	-0.847949333622573
1106	2	-0.7695197092997752
1106	3	-0.7403365194984346
1106	4	-0.6987761692351029
1106	5	-0.7463181340429585
1106	6	-0.7809248571322982
1106	7	-0.7738077280816142
1106	8	-0.8010727073068438
1106	9	-0.9737408698265781
1106	10-11	-1.0459543073067152
1106	12-13	-1.0298329183587498
1106	14-15	-1.2526587805648646
1106	16-17	-1.285318311395642
1106	18-19	-1.2082181882136425
1106	20-21	-1.2610449004710418
1106	22-23	-1.2403041017178893
1106	24-25	-1.1981759798108982
1106	26-27	-1.2395663916722626
1106	28-29	-1.3221309222406532
1106	30-31	-1.314256246185181
1106	32-33	-1.2575473864536946
1106	34-35	-1.2229404621361155
1106	36-37	-1.2662241101989835
1106	38-39	-1.277206815027803
1106	40-41	-1.3447558611473625
1106	42-43	-1.4273879223043267
1106	44-45	-1.3183284466268574
1106	46-47	-1.2775419434881101
1106	48-49	-1.2541168550377364
1106	50-51	-1.2711054383392195
1106	52-53	-1.2788707390926959
1106	54-55	-1.2177645955136995
1106	56-57	-1.160828680132468
1106	58-59	-1.2227448896746758
1106	60-61	-1.205347998060173
1106	62-63	-1.2437941073841507
1106	64-65	-1.1938610771568179
1106	66-67	-1.2332911372573996
1106	68-69	-1.3248181056484754
1106	70-71	-1.2368138526024701
1106	72-73	-1.1837537041824362
1106	74-75	-1.1784801501400821
1106	76-77	-1.1835026193864095
1106	78-79	-1.151977958535607
1106	80-81	-1.03973050350886
1106	82-83	-1.129094992212213
1106	84-85	-1.1047515730149193
1106	86-87	-1.057886891056679
1106	88-89	-1.029319177787304
1106	90-91	-1.0025023997198836
1106	92-93	-1.0221896279504428
1106	94-95	-0.8991728083841295
1106	96-97	-1.0073800545786717
1106	98-99	-1.0818003220782764
1106	100	-1.044742394762995
1107	1	-1.2294286900485396
1107	2	-1.1696748869660922
1107	3	-1.1723285536737293
1107	4	-1.2036473147773066
1107	5	-1.3385161186927164
1107	6	-1.3921492643717883
1107	7	-1.4386267067826992
1107	8	-1.5076303589004212
1107	9	-1.6554406236636083
1107	10-11	-1.704120371485189
1107	12-13	-1.773159256995143
1107	14-15	-2.0177650890281527
1107	16-17	-2.0114604852853404
1107	18-19	-2.020448774026569
1107	20-21	-2.0799507309168135
1107	22-23	-2.2771788291384567
1107	24-25	-2.1637890697324877
1107	26-27	-2.1362740798581115
1107	28-29	-2.148597349081257
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2108	16-17	-2.8568531905358796
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2108	20-21	-2.9342476240691084
2108	22-23	-3.0533417226826813
2108	24-25	-3.2263464587083845
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2108	28-29	-3.1540034206417964
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2108	34-35	-3.1166708742665747
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2208	12-13	-2.223368040247202
2208	14-15	-2.454414376968735
2208	16-17	-2.458512962943651
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2208	20-21	-2.5891657161130723
2208	22-23	-2.7981687327466105
2208	24-25	-2.8621455009433134
2208	26-27	-2.595233297491916
2208	28-29	-2.5064857742120807
2208	30-31	-2.670097983400275
2208	32-33	-2.9113942815512814
2208	34-35	-2.711060566481983
2208	36-37	-2.5709401330119306
2208	38-39	-2.647427814923354
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2308	78-79	-2.457853460091865
2308	80-81	-2.6468364262089565
2308	82-83	-2.4561371032202466
2308	84-85	-2.33467117964139
2308	86-87	-2.5488203831873975
2308	88-89	-2.6847350874334204
2308	90-91	-2.7253361563296927
2308	92-93	-2.510889263464815
2308	94-95	-2.1863052073610376
2308	96-97	-2.380247353164634
2308	98-99	-2.606123804596489
2308	100	-2.95851087626162
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	1147910.0
3	322125.0
4	174755.0
5	141970.0
6	141391.0
7	154700.0
8	173691.0
9	187750.0
10	201490.0
11	202346.0
12	210914.0
13	242902.0
14	306950.0
15	388965.0
16	441560.0
17	403558.0
18	323541.0
19	261522.0
20	244684.0
21	249735.0
22	272733.0
23	311444.0
24	364969.0
25	438047.0
26	523785.0
27	622332.0
28	736272.0
29	868373.0
30	1026817.0
31	1223717.0
32	1484029.0
33	1841304.0
34	2374467.0
35	3232910.0
36	4689514.0
37	6486802.0
38	5674287.0
39	1294764.0
40	2717.0
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	21.880186423314996	27.275150681254694	27.3572504693434	23.487412426086905
2	24.42555532871399	26.786127268001707	26.7345192605836	22.0537981427007
3	24.0883025685942	25.558280717821518	26.333466034581566	24.019950679002722
4	24.868564380244486	25.317503914972583	26.18277206942911	23.631159635353814
5	25.302537556051437	25.340139365832737	25.769346305026886	23.587976773088943
6	25.32413451562407	25.181524848584484	25.176254667924162	24.31808596786728
7	25.230168625901666	25.633593004768667	25.345735929042355	23.790502440287305
8	24.818261451666167	25.325251223979393	25.615373843297327	24.24111348105711
9	24.633422329662665	25.090762673535544	25.73163017893327	24.544184817868523
10-11	24.550458951767197	25.29734159041969	25.720184503531723	24.432014954281392
12-13	24.279813894786752	25.391598364352074	25.8432001517589	24.485387589102274
14-15	24.042673210463324	25.604063371543674	26.2441341097838	24.109129308209198
16-17	23.824879195810087	25.537865231467453	26.482657689385565	24.154597883336894
18-19	23.485713257202395	25.494478216678463	27.102724471061013	23.917084055058126
20-21	23.181750273947966	25.221427255883583	27.880102879788726	23.716719590379725
22-23	23.148562309018146	25.211280890529736	28.24896248453388	23.39119431591824
24-25	23.10561577798824	25.073266844342186	28.578131105808357	23.242986271861213
26-27	23.129554112780944	25.124880784248738	28.632014740103568	23.11355036286675
28-29	23.17379847153333	25.21854491016239	28.57622045091441	23.03143616738987
30-31	23.16200683625421	25.120128210547186	28.533402739248903	23.1844622139497
32-33	23.206252495327565	25.16821366926826	28.477017701527203	23.148516133876974
34-35	23.275330434806783	25.246776304067538	28.428080362275075	23.049812898850607
36-37	23.29788269621401	25.189352270487635	28.331542042993025	23.18122299030533
38-39	23.316249521516564	25.264193240021815	28.226076948374335	23.19348029008729
40-41	23.423533419833348	25.386397902268858	27.99009700914901	23.199971668748777
42-43	23.523876429720584	25.4387698441888	27.614198829383497	23.423154896707114
44-45	23.63992188903573	25.61315845262955	27.224310693435484	23.522608964899238
46-47	23.819509492802457	25.784217278216992	26.824031975926665	23.572241253053885
48-49	23.92033646144924	25.836361662472	26.442449324056525	23.800852552022235
50-51	23.96239707961532	25.89991117062574	26.250384952251704	23.887306797507232
52-53	24.012233114142678	26.011368557273006	26.087230528544925	23.88916780003939
54-55	24.064234894588576	25.955331716960707	26.010942019820625	23.969491368630088
56-57	24.024800915413195	25.9800573525675	26.030123850864133	23.965017881155177
58-59	24.07346704218879	26.086279045641852	25.985648790279757	23.854605121889602
60-61	24.058333245010786	26.046786240798404	25.935473176040503	23.959407338150307
62-63	24.005313253224465	26.14452991609105	25.946847336603557	23.903309494080926
64-65	24.044992180453505	26.298251809947633	25.898176528240676	23.75857948135819
66-67	24.049375223514353	26.269054428072735	25.811067535905156	23.870502812507752
68-69	24.043446074607154	26.37181338769953	25.745538323274047	23.83920221441927
70-71	24.095308244876943	26.483194444174714	25.69649288198853	23.72500442895981
72-73	24.062842337246515	26.50231030538045	25.579149021916248	23.855698335456786
74-75	24.04759984187035	26.60287620033927	25.58480203743286	23.764721920357516
76-77	23.94047880272169	26.81105629323432	25.458737765889655	23.78972713815434
78-79	23.96654448028507	26.85037166861554	25.378915177986922	23.80416867311246
80-81	23.909927493671603	26.938713917562225	25.353335457928832	23.798023130837343
82-83	23.928309644458906	27.069114253357814	25.26854892007904	23.734027182104242
84-85	23.88937257711537	27.103092324587337	25.190511183191806	23.817023915105487
86-87	23.87353187367856	27.228634821370456	25.118718425910046	23.779114879040943
88-89	23.93695541940249	27.334428026723305	25.012464892035	23.71615166183921
90-91	23.939823625005456	27.384204857929	24.901178069443823	23.774793447621718
92-93	23.921603834478	27.477075976972376	24.838064649545235	23.763255539004387
94-95	23.990841479411575	27.63345958922677	24.69834167555262	23.677357255809035
96-97	23.944783523463354	27.694263320180617	24.579782498225285	23.781170658130744
98-99	23.96191292715097	27.790870975246495	24.463899314535656	23.78331678306688
100	24.044589483946705	27.930915946052092	24.099149808887816	23.925344761113383
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	13580.0
1	8536.5
2	3013.5
3	2471.5
4	2461.0
5	2622.0
6	2863.0
7	3162.5
8	3563.0
9	4061.5
10	4822.5
11	5805.0
12	7079.0
13	10064.0
14	17812.0
15	24957.5
16	34659.5
17	36673.5
18	32236.5
19	37321.0
20	46774.5
21	57612.5
22	70895.0
23	88846.5
24	108918.0
25	131024.5
26	157948.5
27	195643.5
28	241590.5
29	286073.0
30	328852.0
31	377562.5
32	435772.0
33	498719.5
34	558550.5
35	627103.5
36	709464.5
37	805740.0
38	913745.5
39	1003659.5
40	1072019.0
41	1149163.0
42	1252538.0
43	1354573.5
44	1425351.5
45	1482381.0
46	1524224.0
47	1539133.5
48	1552091.5
49	1579144.0
50	1558231.5
51	1494684.5
52	1403678.5
53	1294978.5
54	1219981.0
55	1158729.0
56	1090268.0
57	1000472.5
58	919206.5
59	863442.0
60	797560.5
61	723350.0
62	656017.5
63	588613.0
64	518772.0
65	445223.0
66	375323.0
67	313253.0
68	257874.0
69	209421.0
70	165100.5
71	128404.0
72	101180.5
73	80448.0
74	63463.0
75	46855.5
76	31557.0
77	21517.0
78	15856.0
79	10338.0
80	5432.5
81	2603.0
82	1348.0
83	802.5
84	481.5
85	303.0
86	221.5
87	165.0
88	120.5
89	101.5
90	83.5
91	57.0
92	37.5
93	26.5
94	23.5
95	19.5
96	12.5
97	6.0
98	3.5
99	2.5
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.23665873928601583
2	0.001441926584511038
3	0.0
4	5.838787226013006E-5
5	5.331066597664049E-5
6	7.691967519486699E-4
7	0.0043359341661000925
8	0.005559540880421079
9	0.006288119982101833
10-11	0.004616449813262892
12-13	0.007482532760292753
14-15	0.006549596105701546
16-17	0.0034144212256467356
18-19	0.0014546196002197618
20-21	3.477886304190355E-4
22-23	0.0010192491614105313
24-25	0.0011652188420608564
26-27	0.002472599460059421
28-29	0.0017681370882252426
30-31	7.285791016807533E-4
32-33	0.0015764725510235113
34-35	8.847031948980576E-4
36-37	0.0017199036285320919
38-39	9.075506231737606E-4
40-41	0.0015536251227478085
42-43	0.0024357897145041215
44-45	2.259356796152859E-4
46-47	2.741691393084368E-4
48-49	6.232270712983447E-4
50-51	0.001251531348880179
52-53	0.0011779118577695802
54-55	0.001675478073551558
56-57	3.338263131394392E-4
58-59	2.6655332988320245E-5
60-61	3.541351382733975E-4
62-63	1.4343107750858035E-4
64-65	0.0026160305375680006
66-67	0.0027797704402105394
68-69	2.6655332988320245E-4
70-71	0.0011131774776550884
72-73	7.730046566612871E-4
74-75	3.680974555529938E-5
76-77	0.0020321518149667003
78-79	0.010410811484295363
80-81	0.021727904290193615
82-83	0.0016285139154292795
84-85	0.0013975010295305041
86-87	0.0026858421239659824
88-89	0.0028127722810532218
90-91	0.0027099588538125577
92-93	2.4370590160749937E-4
94-95	0.0011309476996473017
96-97	0.0022618953992946033
98-99	0.0016094743918661936
100	0.0012210681111792417
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	3.9391742E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	36.941568316233734
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.77940541150544	26.516438087077653
2	13.724187868500671	10.139860474580876
3	5.378772216714401	5.9610084390364495
4	2.610561267047874	3.85752909561451
5	1.4764718555016998	2.7271592958506212
6	0.9314402123374116	2.0645317341929847
7	0.6160636529318914	1.5930850265352379
8	0.43203560343969183	1.2768058207610113
9	0.32800692524502173	1.0905381213423067
>10	2.1767724754492805	16.408660500788393
>50	0.3214942089538392	8.194483992519059
>100	0.2094079662819031	14.777558557079479
>500	0.012796601318767723	3.3571130477298436
>1k	0.0024383357739305653	1.1486771659518982
>5k	3.46188090650424E-5	0.1040977125945099
>10k+	1.1078018893862762E-4	0.7824529283450066
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	46328	0.11760840635075241	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.538603141744785E-6	0.0	0.0	0.0	0.0
2	1.7770221992213495E-5	2.538603141744785E-6	0.0	0.0	5.07720628348957E-6
3	2.538603141744785E-5	2.538603141744785E-6	0.0	0.0	7.615809425234355E-6
4	3.046323770093742E-5	5.07720628348957E-6	0.0	0.0	7.615809425234355E-6
5	4.315625340966134E-5	7.615809425234355E-6	0.0	0.0	1.015441256697914E-5
6	5.331066597664048E-5	7.615809425234355E-6	0.0	0.0	1.2693015708723925E-5
7	6.092647540187484E-5	1.015441256697914E-5	0.0	0.0	1.2693015708723925E-5
8	7.361949111059876E-5	1.2693015708723925E-5	0.0	0.0	1.523161885046871E-5
9	9.138971310281226E-5	1.2693015708723925E-5	0.0	0.0	1.523161885046871E-5
10-11	1.1677574452026012E-4	1.7770221992213495E-5	0.0	0.0	1.523161885046871E-5
12-13	1.675478073551558E-4	2.030882513395828E-5	0.0	0.0	1.523161885046871E-5
14-15	2.7797704402105394E-4	2.030882513395828E-5	0.0	0.0	1.523161885046871E-5
16-17	5.013741204945951E-4	2.030882513395828E-5	0.0	1.2693015708723925E-6	1.523161885046871E-5
18-19	8.682022744767165E-4	2.411672984657546E-5	0.0	2.538603141744785E-6	1.523161885046871E-5
20-21	0.0013276894431325224	3.173253927180981E-5	0.0	2.538603141744785E-6	1.65009204213411E-5
22-23	0.0020156508945453596	3.5540443984426984E-5	0.0	2.538603141744785E-6	1.7770221992213495E-5
24-25	0.0028736987564550965	3.807904712617177E-5	0.0	2.538603141744785E-6	2.157812670483067E-5
26-27	0.004023685979665484	3.934834869704417E-5	0.0	2.538603141744785E-6	2.538603141744785E-5
28-29	0.005304411264675728	4.061765026791656E-5	0.0	2.538603141744785E-6	2.538603141744785E-5
30-31	0.006182767951719424	4.188695183878895E-5	0.0	2.538603141744785E-6	2.7924634559192636E-5
32-33	0.006712066706773212	4.315625340966134E-5	0.0	2.538603141744785E-6	2.919393613006503E-5
34-35	0.007223595239834786	4.696415812227852E-5	0.0	2.538603141744785E-6	3.30018408426822E-5
36-37	0.007802396756152597	4.823345969315091E-5	0.0	2.538603141744785E-6	3.30018408426822E-5
38-39	0.008292347162509341	5.07720628348957E-5	0.0	2.538603141744785E-6	3.5540443984426984E-5
40-41	0.008748026426452529	5.07720628348957E-5	0.0	5.07720628348957E-6	4.315625340966134E-5
42-43	0.009202436388824845	5.204136440576809E-5	0.0	7.615809425234355E-6	4.315625340966134E-5
44-45	0.009710157017173803	5.331066597664048E-5	0.0	7.615809425234355E-6	4.315625340966134E-5
46-47	0.010535203038240858	5.331066597664048E-5	0.0	7.615809425234355E-6	4.315625340966134E-5
48-49	0.011567145215360113	5.4579967547512874E-5	1.2693015708723925E-6	7.615809425234355E-6	4.569485655140613E-5
50-51	0.012984955070024576	5.711857068925766E-5	2.538603141744785E-6	7.615809425234355E-6	4.569485655140613E-5
52-53	0.014792440506946863	6.473438011449201E-5	2.538603141744785E-6	7.615809425234355E-6	4.696415812227852E-5
54-55	0.017510015170184655	6.85422848271092E-5	2.538603141744785E-6	7.615809425234355E-6	5.07720628348957E-5
56-57	0.02182690981272166	7.361949111059876E-5	2.538603141744785E-6	7.615809425234355E-6	5.331066597664048E-5
58-59	0.027845937861798543	7.361949111059876E-5	2.538603141744785E-6	7.615809425234355E-6	5.584926911838527E-5
60-61	0.03595550559810226	7.361949111059876E-5	2.538603141744785E-6	7.615809425234355E-6	5.584926911838527E-5
62-63	0.04640693473266554	7.488879268147115E-5	2.538603141744785E-6	7.615809425234355E-6	5.965717383100245E-5
64-65	0.06034132737770266	7.615809425234354E-5	2.538603141744785E-6	7.615809425234355E-6	6.092647540187484E-5
66-67	0.07867765787052525	7.615809425234354E-5	2.538603141744785E-6	1.1423714137851533E-5	6.473438011449201E-5
68-69	0.10230697591388571	7.869669739408834E-5	2.538603141744785E-6	1.2693015708723925E-5	7.235018953972637E-5
70-71	0.1318982034356338	7.869669739408834E-5	3.8079047126171776E-6	1.523161885046871E-5	7.361949111059876E-5
72-73	0.16918139847686858	7.869669739408834E-5	5.07720628348957E-6	1.523161885046871E-5	7.361949111059876E-5
74-75	0.21487498572670383	8.37739036775779E-5	5.07720628348957E-6	1.523161885046871E-5	7.361949111059876E-5
76-77	0.27049578056233203	8.50432052484503E-5	5.07720628348957E-6	1.7770221992213495E-5	7.361949111059876E-5
78-79	0.3387753199642707	8.631250681932268E-5	5.07720628348957E-6	1.903952356308589E-5	7.615809425234354E-5
80-81	0.4207975874740447	8.758180839019508E-5	5.07720628348957E-6	2.030882513395828E-5	7.869669739408834E-5
82-83	0.5181809425945164	9.392831624455704E-5	5.07720628348957E-6	2.030882513395828E-5	7.996599896496073E-5
84-85	0.6330920831071649	9.646691938630183E-5	5.07720628348957E-6	2.030882513395828E-5	8.50432052484503E-5
86-87	0.7665032940152786	1.015441256697914E-4	5.07720628348957E-6	2.2847428275703065E-5	8.885110996106747E-5
88	0.8813776247823719	1.0408272881153618E-4	5.07720628348957E-6	2.2847428275703065E-5	9.138971310281226E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATCTCGA	29225	0.0	44.007755	1
TCTCGAG	34175	0.0	37.40626	2
CTCGAGG	43805	0.0	29.021755	3
TCGAGGG	49125	0.0	25.668365	4
TATCTCG	15250	0.0	23.63793	2
GATCCGG	42090	0.0	22.822296	1
TGATACG	4240	0.0	20.949615	3
ATATCTC	17930	0.0	20.704607	1
ATCCGGC	47620	0.0	20.281794	2
TCCGGCG	54045	0.0	17.809614	3
TATGGAT	21155	0.0	17.484196	2
CGAGGGC	72485	0.0	17.435034	5
AGGGCGC	72030	0.0	17.401953	7
ATACGGC	5380	0.0	16.510477	5
GGGCGCG	78220	0.0	16.024836	8
GCGCCGT	59875	0.0	15.848212	9
CGGCGCG	62245	0.0	15.342612	5
GAGGGCG	82760	0.0	15.310153	6
ATATGGA	25745	0.0	14.96861	1
GGATCCG	29555	0.0	14.359548	2
>>END_MODULE