FastQCFastQC Report
Wed 25 May 2016
SRR491036_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR491036_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37618053
Sequences flagged as poor quality0
Sequence length100
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCTCGA314350.047.8638881
TCTCGAG376950.039.4233132
TGATACG41250.032.242683
CTCGAGG463650.031.6049023
ATACGGC44650.030.8401955
TCGAGGG539950.026.9386444
ATATCTC163400.025.8472561
TATCTCG165750.024.9235742
GATCCGG424650.023.0660321
ATCCGGC515650.019.0487382
AGGGCGC782400.018.5975767
CGAGGGC806650.018.1835865
GATACGG79300.017.5423244
GGGCGCG841250.017.4083568
TATGGAT241000.017.1609212
TCCGGCG608800.016.1493723
GGATCCG298200.015.6500812
GAGGGCG957100.015.2908926
GCGCCGT649450.014.9799379
ACGACGC43950.014.81029940-41
Produced by FastQC (version 0.11.5)