FastQCFastQC Report
Wed 25 May 2016
SRR491035_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR491035_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33001818
Sequences flagged as poor quality0
Sequence length100
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCTCGA243350.046.2385251
TCTCGAG293350.037.937642
CTCGAGG375400.029.3821983
TATCTCG115000.029.261052
TCGAGGG427050.025.4653764
GATCCGG355050.022.437791
ATATCTC163050.021.741021
ATCCGGC400950.019.8328382
TGATACG30600.018.2764153
AGGGCGC627550.017.2399627
CGAGGGC643450.017.0764065
GGGCGCG664650.016.3979058
TATGGAT221300.015.9277542
TCCGGCG510700.015.6440273
ATACGGC36800.015.45275
GGATCCG258400.015.1868842
GCGCCGT527050.014.722339
ATGGATC257150.014.5734871
CGGCGCG549850.014.3421595
GAGGGCG773550.014.2105186
Produced by FastQC (version 0.11.5)