FastQCFastQC Report
Wed 25 May 2016
SRR491034_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR491034_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36973487
Sequences flagged as poor quality0
Sequence length100
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCTCGA275550.046.872961
TCTCGAG334800.038.0837822
CTCGAGG415100.030.2971293
TGATACG43900.028.3693583
TCGAGGG478250.026.1491764
ATACGGC50400.024.3376855
GATCCGG404850.022.7860431
TATCTCG175750.019.9756532
ATCCGGC470050.019.6269132
AGGGCGC702000.017.7480057
ACGACGC34050.017.73604440-41
CGAGGGC717600.017.5976435
ATATCTC206900.017.451811
GGGCGCG747650.016.7775218
TCCGGCG564750.016.3105243
TATGGAT238150.015.7086282
GCGCCGT589100.015.1261159
CGGCGCG603200.015.099475
GAGGGCG854350.014.83595856
GGATCCG288750.014.5672242
Produced by FastQC (version 0.11.5)