##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR491034_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 36973487 Sequences flagged as poor quality 0 Sequence length 100 %GC 47 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.64688296778716 31.0 30.0 34.0 26.0 34.0 2 29.98705648185144 31.0 30.0 34.0 26.0 34.0 3 30.104831902925465 31.0 30.0 34.0 26.0 34.0 4 33.5669860946575 35.0 35.0 37.0 28.0 37.0 5 33.077834773874585 35.0 33.0 37.0 26.0 37.0 6 32.95083428295524 35.0 33.0 37.0 26.0 37.0 7 32.874049423577496 35.0 33.0 37.0 26.0 37.0 8 32.8102550078655 35.0 33.0 37.0 25.0 37.0 9 34.11330657019177 38.0 34.0 39.0 25.0 39.0 10-11 34.04653879413645 38.0 34.0 39.0 25.0 39.0 12-13 33.97980301398134 38.0 34.0 39.0 25.0 39.0 14-15 34.93200450636425 39.0 34.0 41.0 25.0 41.0 16-17 34.867555540541794 39.0 34.0 41.0 25.0 41.0 18-19 34.820412732507485 39.0 34.0 41.0 24.0 41.0 20-21 34.75669466880416 39.0 34.0 41.0 23.5 41.0 22-23 34.60264411901426 39.0 34.0 41.0 22.5 41.0 24-25 34.49665553589793 39.0 34.0 41.0 22.0 41.0 26-27 34.29605323674232 38.5 33.5 40.5 20.0 41.0 28-29 34.0700631914972 38.0 33.0 40.0 19.0 41.0 30-31 33.879769711739655 38.0 33.0 40.0 18.0 41.0 32-33 33.635561179285034 38.0 33.0 40.0 16.0 41.0 34-35 33.43128094734479 38.0 33.0 40.0 15.5 41.0 36-37 33.14496579670725 38.0 32.0 40.0 12.0 41.0 38-39 32.97247944182273 38.0 32.0 40.0 10.5 41.0 40-41 32.76105755186142 38.0 31.5 40.0 8.5 41.0 42-43 32.42329863288253 38.0 31.0 40.0 7.0 41.0 44-45 32.06218899504934 37.0 31.0 40.0 2.0 41.0 46-47 31.93253967363154 37.0 30.5 40.0 2.0 41.0 48-49 31.716577097529374 37.0 30.0 40.0 2.0 41.0 50-51 31.47273185242171 37.0 30.0 40.0 2.0 41.0 52-53 31.20670357653851 36.5 30.0 40.0 2.0 41.0 54-55 30.92734950858165 36.0 29.0 40.0 2.0 41.0 56-57 30.626513006468663 36.0 29.0 40.0 2.0 41.0 58-59 30.317000070888636 35.0 28.0 39.0 2.0 41.0 60-61 30.003182185656442 35.0 28.0 39.0 2.0 41.0 62-63 29.751327025227564 35.0 28.0 39.0 2.0 40.5 64-65 29.688117812636932 35.0 28.0 39.0 2.0 40.0 66-67 29.407520029149538 35.0 28.0 38.0 2.0 40.0 68-69 29.04244258865819 34.5 27.5 37.5 2.0 40.0 70-71 28.691615548730905 34.0 26.5 37.0 2.0 39.5 72-73 28.330045540470664 34.0 26.0 36.0 2.0 39.0 74-75 27.974163810949182 34.0 26.0 36.0 2.0 39.0 76-77 27.189912720431266 33.0 25.5 35.0 2.0 37.0 78-79 27.31861714855296 34.0 26.0 35.0 2.0 37.0 80-81 27.1529399296312 34.0 25.5 35.0 2.0 37.0 82-83 26.86946492225632 34.0 25.0 35.0 2.0 36.0 84-85 26.56584671064431 33.0 24.5 35.0 2.0 36.0 86-87 26.32784627535942 33.0 24.0 35.0 2.0 36.0 88-89 26.097643670990514 33.0 23.0 35.0 2.0 35.0 90-91 25.84917201615309 33.0 21.5 35.0 2.0 35.0 92-93 25.585420317537267 33.0 20.0 35.0 2.0 35.0 94-95 25.322191128469978 33.0 18.5 35.0 2.0 35.0 96-97 24.99348768483752 33.0 12.0 35.0 2.0 35.0 98-99 24.65169442903776 33.0 2.0 35.0 2.0 35.0 100 23.898363657179534 32.0 2.0 34.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.5408726159401738 1101 2 0.5131093048396274 1101 3 0.50803429976947 1101 4 0.5426498456606552 1101 5 0.6856150830247358 1101 6 0.732666151307555 1101 7 0.764799258613813 1101 8 0.7722241352917507 1101 9 0.8540614358713583 1101 10-11 0.9005821153476603 1101 12-13 0.8634687281433244 1101 14-15 0.9234411494983377 1101 16-17 0.8219821108160659 1101 18-19 0.7999418257040745 1101 20-21 0.8423304732438268 1101 22-23 0.8278983934795647 1101 24-25 0.905848551569953 1101 26-27 0.9091439745360645 1101 28-29 0.8582218807190287 1101 30-31 0.8211369074162249 1101 32-33 0.9015987333388438 1101 34-35 0.9163438157618486 1101 36-37 0.9387970631590719 1101 38-39 0.7994056672050576 1101 40-41 0.7816886128750653 1101 42-43 0.7876231297099281 1101 44-45 0.7048559389647977 1101 46-47 0.6358383438630852 1101 48-49 0.6395884164713586 1101 50-51 0.5369114745528627 1101 52-53 0.6715982960115419 1101 54-55 0.4923894737564929 1101 56-57 0.4775884848263239 1101 58-59 0.4934684323827838 1101 60-61 0.5266647339476478 1101 62-63 0.5486187772517752 1101 64-65 0.5299347261791354 1101 66-67 0.6005535046020931 1101 68-69 0.6366879087716697 1101 70-71 0.6565473564054223 1101 72-73 0.5962976025361435 1101 74-75 0.5376959479463714 1101 76-77 0.5691875077640312 1101 78-79 0.6244475252927799 1101 80-81 0.6007013958874197 1101 82-83 0.500567621815506 1101 84-85 0.470450646481666 1101 86-87 0.3816169126734934 1101 88-89 0.43467002078310557 1101 90-91 0.37083904508059007 1101 92-93 0.31235737045575007 1101 94-95 0.2245272136167351 1101 96-97 0.19814688587283413 1101 98-99 0.16411093424824585 1101 100 0.15657646025668726 1102 1 0.23342785080300743 1102 2 0.19233078450493224 1102 3 0.22572279437225973 1102 4 0.2921739712922147 1102 5 0.4138865147469346 1102 6 0.41339140454822143 1102 7 0.3793469454051319 1102 8 0.28568333845075955 1102 9 0.2582497108133168 1102 10-11 0.34909020071773966 1102 12-13 0.38673650325309694 1102 14-15 0.35704859749986895 1102 16-17 0.2424961998948163 1102 18-19 0.36887439823697576 1102 20-21 0.4467389476482069 1102 22-23 0.37435132494622536 1102 24-25 0.3965835598638492 1102 26-27 0.4072386729495747 1102 28-29 0.42485469859995817 1102 30-31 0.3815880407758243 1102 32-33 0.35080359517638726 1102 34-35 0.3399384979184461 1102 36-37 0.48491377568588234 1102 38-39 0.4631825953386226 1102 40-41 0.31446517759558645 1102 42-43 0.3032659010101213 1102 44-45 0.3706793199203915 1102 46-47 0.24967331235426116 1102 48-49 0.026929488062950924 1102 50-51 0.040502435838615014 1102 52-53 0.20185488991645428 1102 54-55 0.3033498501613572 1102 56-57 0.1589995778816018 1102 58-59 -0.038113232154074694 1102 60-61 0.06946219529758935 1102 62-63 0.13295247507628005 1102 64-65 0.20619625018415277 1102 66-67 0.09308997173933875 1102 68-69 0.18551150098689462 1102 70-71 0.1120474241710987 1102 72-73 0.18863868779987314 1102 74-75 0.05484318510758612 1102 76-77 0.07798952822261995 1102 78-79 0.08546053873445913 1102 80-81 0.14999975635926077 1102 82-83 0.12388576757061998 1102 84-85 0.09205001702493476 1102 86-87 -0.029564598102240325 1102 88-89 0.08089534591289294 1102 90-91 0.14343398996959422 1102 92-93 0.044166559178108855 1102 94-95 0.07639758728022628 1102 96-97 -0.042970966445533776 1102 98-99 0.005238834668030989 1102 100 -0.018631907492395072 1103 1 -0.02184130950206864 1103 2 -0.06715415214780407 1103 3 6.240663477292685E-4 1103 4 -0.021953400706721027 1103 5 0.045715913991827506 1103 6 0.03809521757249712 1103 7 0.017316352583641503 1103 8 0.009933685696630334 1103 9 -0.1148037127675039 1103 10-11 -0.14452942383104528 1103 12-13 -0.047803492952333215 1103 14-15 -0.12920891303610915 1103 16-17 -0.03407776718846378 1103 18-19 -0.10364721869556348 1103 20-21 -0.04548342549578166 1103 22-23 -0.09150905684165167 1103 24-25 0.021457927269644017 1103 26-27 -0.026764857051794877 1103 28-29 -0.051124061588481595 1103 30-31 -0.07071498939554743 1103 32-33 -0.023507501775043238 1103 34-35 -0.02322902365002477 1103 36-37 0.05915778785050918 1103 38-39 -2.177483041307937E-4 1103 40-41 -0.08329584737911233 1103 42-43 -0.06805147429740543 1103 44-45 -0.10322712340547469 1103 46-47 -0.0561515611572716 1103 48-49 -0.17310486353274968 1103 50-51 -0.18439589201074114 1103 52-53 -0.1281466343887523 1103 54-55 -0.09378208203522931 1103 56-57 -0.1952892366656087 1103 58-59 -0.0680986169843294 1103 60-61 -0.07202486157381571 1103 62-63 -0.042440666500851876 1103 64-65 -0.013280884803027249 1103 66-67 0.008884192027558413 1103 68-69 0.022562538417250977 1103 70-71 0.05694639209169239 1103 72-73 -0.03995224172378542 1103 74-75 0.003939296760879785 1103 76-77 0.04496076827386375 1103 78-79 0.08185827409387514 1103 80-81 0.06946694006212795 1103 82-83 0.039508141738117786 1103 84-85 -3.8550748087118336E-4 1103 86-87 -0.00687979912374459 1103 88-89 0.006913052170727241 1103 90-91 -0.07115520513980655 1103 92-93 -0.004776808387930487 1103 94-95 -0.03315583064949479 1103 96-97 -0.12050202466708271 1103 98-99 -0.2495409341353252 1103 100 -0.1626552703212134 1104 1 -0.31859486645625523 1104 2 -0.3126621464385799 1104 3 -0.34202166884920615 1104 4 -0.22110175378787744 1104 5 -0.32072211657374794 1104 6 -0.3858007539276258 1104 7 -0.3274250464379307 1104 8 -0.21719329121560094 1104 9 -0.3331525743629413 1104 10-11 -0.4512877947569365 1104 12-13 -0.4193849359388153 1104 14-15 -0.45006936947945775 1104 16-17 -0.3992776570688932 1104 18-19 -0.5017316978403201 1104 20-21 -0.4223290484989164 1104 22-23 -0.3781698255367658 1104 24-25 -0.45288004484132927 1104 26-27 -0.47978089802736434 1104 28-29 -0.46170020955896973 1104 30-31 -0.35153477669307165 1104 32-33 -0.3654549842151198 1104 34-35 -0.39154067271022086 1104 36-37 -0.4169526185802468 1104 38-39 -0.4776606968067654 1104 40-41 -0.5439671944527262 1104 42-43 -0.5367487341544077 1104 44-45 -0.5032727461745097 1104 46-47 -0.5316954339486948 1104 48-49 -0.5238262607964117 1104 50-51 -0.4616417298786146 1104 52-53 -0.5408736111473864 1104 54-55 -0.6764985203885843 1104 56-57 -0.45487817258279506 1104 58-59 -0.3848841473874778 1104 60-61 -0.4214550986317036 1104 62-63 -0.5531094088578818 1104 64-65 -0.4909004679226463 1104 66-67 -0.4498441613201898 1104 68-69 -0.49245429541106844 1104 70-71 -0.5168603271931964 1104 72-73 -0.4417323596444369 1104 74-75 -0.5115094379455449 1104 76-77 -0.5039885509740465 1104 78-79 -0.5245145380267573 1104 80-81 -0.5389481984956674 1104 82-83 -0.6047482623448097 1104 84-85 -0.5857608543309212 1104 86-87 -0.5910437214859705 1104 88-89 -0.5885418826421223 1104 90-91 -0.5772695028941648 1104 92-93 -0.6071164603443933 1104 94-95 -0.5914644983629671 1104 96-97 -0.8354303312482116 1104 98-99 -0.7649605107791828 1104 100 -0.7450675643006228 1105 1 -0.7102075145541917 1105 2 -0.6988785534766109 1105 3 -0.7152862752880935 1105 4 -0.5871164774201887 1105 5 -0.6757083738760912 1105 6 -0.8709630758342684 1105 7 -0.7558254837214236 1105 8 -0.774908542568923 1105 9 -0.933058968096141 1105 10-11 -0.8859094643722258 1105 12-13 -1.0270218734798675 1105 14-15 -1.1488838446169822 1105 16-17 -1.0694639951803282 1105 18-19 -1.029683974851551 1105 20-21 -1.0085470646098926 1105 22-23 -0.9811289330230366 1105 24-25 -0.9706137171057136 1105 26-27 -1.060684582108955 1105 28-29 -1.088302563758262 1105 30-31 -1.045266836199552 1105 32-33 -1.0457327488776968 1105 34-35 -1.0090946045785145 1105 36-37 -0.9042198613295582 1105 38-39 -1.0174176837901427 1105 40-41 -1.0831674625786647 1105 42-43 -0.9960630142912592 1105 44-45 -0.9524288274126853 1105 46-47 -0.9352546207151136 1105 48-49 -0.938441467252062 1105 50-51 -0.8996018782347797 1105 52-53 -0.9785913817330218 1105 54-55 -0.9467763480791795 1105 56-57 -0.9728245472585257 1105 58-59 -0.9082159751036478 1105 60-61 -0.8655877898086857 1105 62-63 -0.883348651174316 1105 64-65 -0.9183142028022147 1105 66-67 -0.9053270851235986 1105 68-69 -0.8420756936052136 1105 70-71 -0.8591613436400003 1105 72-73 -0.8275247622048703 1105 74-75 -0.8890455326446762 1105 76-77 -0.8462550596304474 1105 78-79 -0.7683349155447452 1105 80-81 -0.7106283881667323 1105 82-83 -0.7251599682387422 1105 84-85 -0.6699941837718768 1105 86-87 -0.7062720496819352 1105 88-89 -0.7917756071768949 1105 90-91 -0.9173270045508453 1105 92-93 -0.7792047917422025 1105 94-95 -0.7612120755835221 1105 96-97 -0.7271715592191796 1105 98-99 -0.7206922749399034 1105 100 -0.7463159756987068 1106 1 -1.1790397085953401 1106 2 -1.2103200688302778 1106 3 -1.1911368152320705 1106 4 -1.1983289802869166 1106 5 -1.2946256107500531 1106 6 -1.3243227636574275 1106 7 -1.4319604085337723 1106 8 -1.3902052850008175 1106 9 -1.6934333010804323 1106 10-11 -1.625341814670847 1106 12-13 -1.6698837610734572 1106 14-15 -1.8815857148936033 1106 16-17 -1.9012043452293739 1106 18-19 -1.9810708181246994 1106 20-21 -1.8656852332389562 1106 22-23 -1.9771264863748783 1106 24-25 -1.9280715084501523 1106 26-27 -1.9921296337637742 1106 28-29 -2.017191192660164 1106 30-31 -1.9796239077480067 1106 32-33 -1.9853519272756088 1106 34-35 -1.980415997960037 1106 36-37 -1.8338772199615114 1106 38-39 -1.9862651052638327 1106 40-41 -1.999851801698135 1106 42-43 -2.053779019222304 1106 44-45 -2.023549652828059 1106 46-47 -2.0088324189773843 1106 48-49 -1.9340475862764421 1106 50-51 -1.982335473394258 1106 52-53 -1.9844814905248391 1106 54-55 -1.9768681464000437 1106 56-57 -2.058625103066081 1106 58-59 -2.020652167801728 1106 60-61 -2.019052478558237 1106 62-63 -1.9416221006004406 1106 64-65 -1.8664297773328435 1106 66-67 -2.052123889800434 1106 68-69 -1.9344161220049365 1106 70-71 -1.842481197625304 1106 72-73 -1.9125381096129495 1106 74-75 -1.8698503952189576 1106 76-77 -1.8926486251250303 1106 78-79 -1.7877814237364866 1106 80-81 -1.7560342626128147 1106 82-83 -1.7376550367276167 1106 84-85 -1.856371585901666 1106 86-87 -1.848466305267145 1106 88-89 -1.7901193231118349 1106 90-91 -1.7687468126470236 1106 92-93 -1.7823310858888632 1106 94-95 -1.7717783774770908 1106 96-97 -1.7836673005219161 1106 98-99 -1.8363187333458413 1106 100 -1.854868086894605 1107 1 -1.731387209106618 1107 2 -1.6814514237058305 1107 3 -1.702577123821925 1107 4 -1.7652565062769483 1107 5 -2.0938140348615057 1107 6 -2.115727039372743 1107 7 -2.1600949509735905 1107 8 -2.1286571051258605 1107 9 -2.3364080139378878 1107 10-11 -2.409321891722218 1107 12-13 -2.4496891854454645 1107 14-15 -2.7222294007800656 1107 16-17 -2.7726533091404093 1107 18-19 -2.8230446649700482 1107 20-21 -2.8175312354419546 1107 22-23 -2.8851936193874153 1107 24-25 -2.801111688896782 1107 26-27 -2.7780146338923473 1107 28-29 -2.779852197437762 1107 30-31 -2.894791218161828 1107 32-33 -3.0741707827820832 1107 34-35 -2.9708740332092205 1107 36-37 -2.835227255690299 1107 38-39 -3.0093802569302106 1107 40-41 -3.1345177264204267 1107 42-43 -3.0093795414318834 1107 44-45 -3.067917782228598 1107 46-47 -3.0196596026555014 1107 48-49 -3.013040497636272 1107 50-51 -2.978290071487965 1107 52-53 -2.944732199701594 1107 54-55 -2.8927673061224404 1107 56-57 -3.0079887101931213 1107 58-59 -3.0347825604418723 1107 60-61 -3.0063805798992647 1107 62-63 -2.9968331941353554 1107 64-65 -3.134387009552963 1107 66-67 -3.001388288469794 1107 68-69 -2.969954020045197 1107 70-71 -2.981865994317374 1107 72-73 -2.862344787978376 1107 74-75 -3.0701609549219917 1107 76-77 -3.1400572131657682 1107 78-79 -3.085392655203968 1107 80-81 -3.003395835731105 1107 82-83 -3.115583667171471 1107 84-85 -3.065099810965446 1107 86-87 -3.125140389611836 1107 88-89 -2.994823662812717 1107 90-91 -2.9894905279704425 1107 92-93 -2.9885735964755327 1107 94-95 -3.04750654699237 1107 96-97 -3.082282107824131 1107 98-99 -3.167476457810249 1107 100 -3.105213044383742 1108 1 -2.456845925705405 1108 2 -2.5527329371785505 1108 3 -2.4404376147394338 1108 4 -2.600079867114136 1108 5 -3.0953296349527903 1108 6 -3.1154787538986426 1108 7 -3.1759635897701024 1108 8 -3.2886843511261077 1108 9 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2306 8 -0.1520495146099634 2306 9 -0.06922797294067351 2306 10-11 0.06520639543312257 2306 12-13 0.009404162168515029 2306 14-15 -0.02444767627383726 2306 16-17 0.0054047809957182835 2306 18-19 0.005091132475058657 2306 20-21 0.07960258587721825 2306 22-23 0.11973777627896709 2306 24-25 0.05975315288078065 2306 26-27 0.10650786095799702 2306 28-29 0.06372534282127162 2306 30-31 0.05979456613610523 2306 32-33 0.16745705905515962 2306 34-35 0.22458771408688705 2306 36-37 0.3167004610515036 2306 38-39 0.28425064594362937 2306 40-41 0.17458359049484073 2306 42-43 0.22480170593518523 2306 44-45 0.2016092664127953 2306 46-47 0.09392417814777332 2306 48-49 0.17382290818656898 2306 50-51 0.17197389132823204 2306 52-53 0.20664061660538025 2306 54-55 0.32888351252745096 2306 56-57 0.3428187816534347 2306 58-59 0.31336945360547475 2306 60-61 0.290755706302793 2306 62-63 0.314378002171086 2306 64-65 0.25801219005926157 2306 66-67 0.25581290459441774 2306 68-69 0.2988872498073185 2306 70-71 0.3328228804275639 2306 72-73 0.34067818507532266 2306 74-75 0.34530135852071453 2306 76-77 0.3563700029321417 2306 78-79 0.3615394525427078 2306 80-81 0.2648607458650005 2306 82-83 0.3463286965486425 2306 84-85 0.39524044330370955 2306 86-87 0.3447575238741436 2306 88-89 0.35414151838432417 2306 90-91 0.40638143746240374 2306 92-93 0.4051073354449244 2306 94-95 0.47611911322600875 2306 96-97 0.5593617710604555 2306 98-99 0.5639696692546572 2306 100 0.5640602794151945 2307 1 -0.56073474084711 2307 2 -0.5281922352535418 2307 3 -0.530781180001263 2307 4 -0.5004955860471085 2307 5 -0.5905098697059543 2307 6 -0.5563402223288847 2307 7 -0.6169331910589477 2307 8 -0.6423807252177909 2307 9 -1.0360223904863233 2307 10-11 -0.7354259485140915 2307 12-13 -0.7236200062737197 2307 14-15 -0.7753901547192683 2307 16-17 -0.7855901719036922 2307 18-19 -0.8892049306788223 2307 20-21 -0.8986267573376878 2307 22-23 -0.7294554118490382 2307 24-25 -0.8167482378774835 2307 26-27 -0.7963530835136012 2307 28-29 -0.7893657588127567 2307 30-31 -0.7787228034003704 2307 32-33 -0.7877103968849326 2307 34-35 -1.0108877009665989 2307 36-37 -0.8829292551327299 2307 38-39 -0.8475420743608737 2307 40-41 -0.7636239455820117 2307 42-43 -0.8337564921429603 2307 44-45 -0.7887616246618627 2307 46-47 -0.7776032050504469 2307 48-49 -0.7318021457432415 2307 50-51 -0.776083977131055 2307 52-53 -0.7674377278246105 2307 54-55 -0.8288918897106221 2307 56-57 -0.8991957019458141 2307 58-59 -0.7971471170738482 2307 60-61 -0.7193266390599504 2307 62-63 -0.697272005778661 2307 64-65 -1.0033798675096754 2307 66-67 -0.8272225554693371 2307 68-69 -0.7875111051283703 2307 70-71 -0.8136276590609341 2307 72-73 -0.8415839208378557 2307 74-75 -0.8981609246642854 2307 76-77 -0.9206281054871006 2307 78-79 -0.8721652161854578 2307 80-81 -0.9167066657158571 2307 82-83 -0.9138517352299758 2307 84-85 -1.0124090261124223 2307 86-87 -0.9453930461335567 2307 88-89 -0.9375748655415244 2307 90-91 -0.9832333751424365 2307 92-93 -0.9574999153601098 2307 94-95 -0.9314730508151818 2307 96-97 -0.8313198624217186 2307 98-99 -0.881587252870812 2307 100 -0.895383583511336 2308 1 -1.690409183515463 2308 2 -1.6458130112733613 2308 3 -1.6088972823269891 2308 4 -1.6032171147763634 2308 5 -1.909425953035786 2308 6 -1.853849347669886 2308 7 -1.9627704033179931 2308 8 -2.0398837455173577 2308 9 -2.2013150641986527 2308 10-11 -2.31047973350082 2308 12-13 -2.3639006375932183 2308 14-15 -2.638598560799167 2308 16-17 -2.5809878429741246 2308 18-19 -2.6476687341416394 2308 20-21 -2.774703393904389 2308 22-23 -2.832113955707431 2308 24-25 -2.8280809299855605 2308 26-27 -2.6654952707717285 2308 28-29 -2.5990635107650775 2308 30-31 -2.5959032903576436 2308 32-33 -2.5760018424219666 2308 34-35 -2.583936023958106 2308 36-37 -2.832819136804524 2308 38-39 -2.714399308613686 2308 40-41 -2.79663083137617 2308 42-43 -2.975986984189042 2308 44-45 -2.9701584059719295 2308 46-47 -2.6169380866998075 2308 48-49 -2.7220844320093356 2308 50-51 -2.565520223665324 2308 52-53 -2.753422676909512 2308 54-55 -2.6909783071524913 2308 56-57 -2.648038494910004 2308 58-59 -2.600549928464577 2308 60-61 -2.587194310148554 2308 62-63 -2.852191922524696 2308 64-65 -2.915351025381799 2308 66-67 -3.18543270326904 2308 68-69 -2.964417932596909 2308 70-71 -2.8454758985170265 2308 72-73 -2.700805697627029 2308 74-75 -2.811187089316519 2308 76-77 -2.775643804388441 2308 78-79 -2.6812575311219895 2308 80-81 -2.6291330079902053 2308 82-83 -2.4878536594537977 2308 84-85 -2.537112707454977 2308 86-87 -2.7321960119863107 2308 88-89 -2.8565184166111557 2308 90-91 -2.6062892230461365 2308 92-93 -2.5031654959245166 2308 94-95 -2.365324052708491 2308 96-97 -2.8439513280543167 2308 98-99 -2.6951329542495976 2308 100 -2.5925975627272884 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1361807.0 3 372212.0 4 193503.0 5 152583.0 6 148668.0 7 164720.0 8 187962.0 9 203476.0 10 213958.0 11 214221.0 12 221467.0 13 255289.0 14 316161.0 15 395101.0 16 440201.0 17 398980.0 18 323284.0 19 266574.0 20 250569.0 21 254902.0 22 277718.0 23 317939.0 24 377218.0 25 452404.0 26 543037.0 27 645392.0 28 758872.0 29 892765.0 30 1051855.0 31 1246992.0 32 1503376.0 33 1839678.0 34 2327773.0 35 3068767.0 36 4260536.0 37 5592996.0 38 4587692.0 39 891353.0 40 1486.0 >>END_MODULE >>Per base sequence content pass #Base G A T C 1 21.823055344614627 27.37580091654387 27.460135691419147 23.34100804742236 2 24.34971081850065 27.159359621393424 26.726661139695018 21.764268420410904 3 24.116738029063907 25.867949647270216 26.287112167700062 23.728200155965816 4 24.91563334137785 25.44826984283553 26.17082108658939 23.46527572919723 5 25.223248733803665 25.521114758003698 25.911193185878233 23.3444433223144 6 25.254862327524318 25.360049143799873 25.297418495363853 24.08767003331195 7 25.254323369479444 25.76981459988856 25.497176366653985 23.478685663978016 8 24.789411436116822 25.490546539487024 25.778467533362587 23.94157449103357 9 24.584601713090652 25.20233645417213 25.909068643315415 24.303993189421803 10-11 24.519289163156337 25.419196083980207 25.847590202782772 24.213924550080687 12-13 24.252316429263793 25.54944703086628 25.955398039683498 24.24283850018643 14-15 23.954494913895747 25.759796913414768 26.393079233646326 23.892628939043163 16-17 23.769669703449285 25.66979520182116 26.65737248463446 23.903162610095098 18-19 23.394883091514917 25.64312240901233 27.320825279515375 23.641169219957376 20-21 23.053338436959802 25.382127900913083 28.12156923720128 23.442964424925833 22-23 23.06621808114287 25.346203247304672 28.52236543539886 23.065213236153596 24-25 22.950416960300362 25.2072724359361 28.925440510051942 22.91687009371159 26-27 22.99011297444592 25.273838736554072 28.959716150463777 22.776332138536233 28-29 23.033161888905067 25.350319858217368 28.911811259071463 22.704706993806102 30-31 23.001563891985043 25.245020473574343 28.90223346214926 22.851182172291352 32-33 23.048019790297126 25.297535740689387 28.81361745336181 22.840827015651676 34-35 23.155540352419106 25.356458077461692 28.786816083870676 22.70118548624852 36-37 23.26887117253314 25.270139863700564 28.651462162851338 22.809526800914956 38-39 23.160508012397234 25.39395926201366 28.601959717504016 22.843573008085087 40-41 23.231813468290436 25.5123453928351 28.398253840216963 22.857587298657503 42-43 23.3599906998053 25.55399031610194 27.985935511166794 23.100083472925963 44-45 23.431699783577148 25.778150158304552 27.59379599364283 23.19635406447548 46-47 23.67756764170796 25.931953142061285 27.144909264418686 23.245569951812065 48-49 23.749756723989353 25.983235725180382 26.75879241189547 23.508215138934794 50-51 23.770681146890436 26.05831574355272 26.573653186114132 23.597349923442717 52-53 23.851715224263966 26.170934969742515 26.377158099219685 23.600191706773835 54-55 23.915099335074068 26.138472508624595 26.264665585506304 23.681762570795037 56-57 23.87785922333662 26.15577796241434 26.289632932122746 23.676729882126292 58-59 23.950847876590153 26.248597209048153 26.238005361433142 23.56254955292855 60-61 23.909937999454158 26.211502653164203 26.198721419536735 23.6798379278449 62-63 23.88037074503233 26.327769348522285 26.21184948588318 23.5800104205622 64-65 23.908306818623945 26.444061798921375 26.183625160461226 23.464006221993454 66-67 23.888145629107537 26.44443470431303 26.115852678309466 23.55156698826997 68-69 23.927089543835912 26.54926307573294 26.01479617767844 23.5088512027527 70-71 23.96446839329446 26.68482584234091 25.954711811250313 23.39599395311432 72-73 23.91432033494018 26.709609650063364 25.82504340849665 23.551026606499807 74-75 23.903599415672073 26.846400787182002 25.79405740337587 23.455942393770055 76-77 23.828164868482013 27.014489028139653 25.66544110900128 23.49190499437705 78-79 23.8439554664636 27.04747392254923 25.603562163614647 23.505008447372525 80-81 23.76835892514123 27.15301743571344 25.585523738658072 23.49309990048726 82-83 23.787752276847517 27.271906039198413 25.50358257624615 23.43675910770792 84-85 23.7432907164762 27.310562305438285 25.411808490488102 23.53433848759742 86-87 23.72602110392254 27.47938792381545 25.315531757509874 23.479059214752134 88-89 23.824579728413816 27.552653442062873 25.21092069748007 23.41184613204324 90-91 23.782536495593742 27.580184660860102 25.16401470910293 23.473264134443227 92-93 23.813414109008242 27.71060664075919 25.051879219537465 23.424100030695104 94-95 23.853324618636094 27.816038873669513 24.9552187562153 23.375417751479098 96-97 23.804491644285132 27.910792766308383 24.7806935127511 23.50402207665538 98-99 23.781033525205718 28.01713246897628 24.682748183950608 23.519085821867396 100 23.926078829221712 28.11601252901383 24.321776742027406 23.63613189973705 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 1648.0 1 1108.0 2 564.0 3 574.5 4 660.0 5 760.5 6 860.5 7 1044.5 8 1255.5 9 1510.0 10 1883.5 11 2376.5 12 3122.0 13 4682.0 14 8811.0 15 12979.5 16 18736.5 17 21662.0 18 21630.0 19 26099.5 20 33976.0 21 42896.0 22 54107.5 23 68102.5 24 82458.0 25 98703.0 26 119221.0 27 147143.0 28 180324.0 29 213751.5 30 249614.5 31 297495.5 32 360415.5 33 426672.5 34 489048.5 35 572480.0 36 671240.0 37 789231.5 38 913275.5 39 998632.0 40 1068077.0 41 1160596.5 42 1306200.5 43 1443903.5 44 1543198.0 45 1629726.5 46 1664069.5 47 1660281.5 48 1657438.0 49 1664661.0 50 1622229.0 51 1523318.5 52 1386169.5 53 1232192.5 54 1122273.5 55 1036099.0 56 938629.5 57 834145.5 58 758001.5 59 701141.0 60 629074.5 61 558384.5 62 495462.0 63 431762.0 64 374624.0 65 320994.0 66 270159.5 67 223706.0 68 182407.5 69 145434.0 70 112804.0 71 85980.5 72 66791.5 73 52724.0 74 40690.0 75 29419.0 76 20187.5 77 14370.0 78 10740.5 79 6971.0 80 3738.0 81 1905.0 82 992.5 83 595.5 84 380.0 85 246.5 86 173.0 87 135.0 88 98.5 89 66.0 90 51.5 91 49.5 92 35.0 93 18.0 94 14.5 95 8.5 96 4.5 97 3.5 98 1.0 99 1.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.29987974896714503 2 0.002323286413315574 3 0.0 4 1.4605060106989638E-4 5 4.1381003636470646E-4 6 0.003172543612129416 7 0.005814977635190319 8 0.006980677802988936 9 0.007475627062170253 10-11 0.006151705409879246 12-13 0.009156561294854338 14-15 0.008779263908757105 16-17 0.012120847568421123 18-19 0.007214629228776826 20-21 0.006465443738103469 22-23 0.007060464705425268 24-25 0.0076297915855218085 26-27 0.007881323176253298 28-29 0.007598688216775442 30-31 0.007851572127887207 32-33 0.007693350643394819 34-35 0.008236983436266101 36-37 0.0062598910403013925 38-39 0.006334268661216618 40-41 0.006840036483440147 42-43 0.005998893206907966 44-45 0.006342382583498277 46-47 0.005390349035783398 48-49 0.004307140411181666 50-51 0.004052904179689625 52-53 0.003779735462873707 54-55 0.0031738959325096927 56-57 0.004747996855151909 58-59 0.004478885099476822 60-61 0.0035498409982266483 62-63 0.0037689168998314925 64-65 0.002083925706006577 66-67 0.004307140411181666 68-69 0.004073188985393777 70-71 0.004673619234236684 72-73 0.004734473651349141 74-75 0.002966990914327339 76-77 0.0036201616580010423 78-79 0.0012224976237702437 80-81 0.013126973931347076 82-83 0.015730190663379953 84-85 0.017377316886557116 86-87 0.01250896351756057 88-89 0.006554696883201739 90-91 0.0019906155997674764 92-93 0.004856182485574055 94-95 0.0018864869304861616 96-97 0.0023192294521747437 98-99 0.0017553118535993104 100 0.009785390271683058 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 3.6973487E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 35.35274001214356 #Duplication Level Percentage of deduplicated Percentage of total 1 74.3100331997406 26.270632840041863 2 12.981445990053468 9.178593701360878 3 4.870026484685559 5.165063404960261 4 2.317641959792695 3.2773997458314414 5 1.2654624538578734 2.236878256318331 6 0.79205475899977 1.680078358217994 7 0.4874597211024425 1.2063125750568664 8 0.37457145521723334 1.0593701817812102 9 0.24859052369141113 0.7909520538190564 >10 1.755608298356389 13.300703700393528 >50 0.33200796390686077 8.087379560971915 >100 0.22532222733193086 17.438254829483 >500 0.03757807546113509 8.611172765717823 >1k 0.0020427521797385024 0.9038384464118934 >5k 6.165424943431988E-5 0.17321379441313836 >10k+ 9.248137348785159E-5 0.620155785220945 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 2.434176684498273E-5 0.0 0.0 2.7046407605536368E-6 0.0 2 3.786497064775091E-5 0.0 0.0 2.7046407605536368E-6 0.0 3 5.13881744505191E-5 0.0 0.0 5.4092815211072736E-6 2.7046407605536368E-6 4 7.302530053494819E-5 2.7046407605536368E-6 0.0 8.11392228166091E-6 2.7046407605536368E-6 5 1.027763489010382E-4 2.7046407605536368E-6 0.0 1.0818563042214547E-5 2.7046407605536368E-6 6 1.1900419346436001E-4 2.7046407605536368E-6 0.0 1.0818563042214547E-5 2.7046407605536368E-6 7 1.4605060106989638E-4 5.4092815211072736E-6 0.0 1.8932485323875456E-5 2.7046407605536368E-6 8 1.622784456332182E-4 5.4092815211072736E-6 0.0 2.7046407605536368E-5 2.7046407605536368E-6 9 1.8121093095709365E-4 5.4092815211072736E-6 0.0 2.9751048366090003E-5 2.7046407605536368E-6 10-11 2.217805423653982E-4 5.4092815211072736E-6 0.0 3.3808009506920464E-5 2.7046407605536368E-6 12-13 2.934535225200696E-4 5.4092815211072736E-6 0.0 3.921729102802773E-5 2.7046407605536368E-6 14-15 4.570842885335646E-4 5.4092815211072736E-6 2.7046407605536368E-6 4.597889292941183E-5 5.4092815211072736E-6 16-17 7.329576461100355E-4 5.4092815211072736E-6 2.7046407605536368E-6 4.868353368996546E-5 5.4092815211072736E-6 18-19 0.0011224259156297593 5.4092815211072736E-6 2.7046407605536368E-6 4.868353368996546E-5 6.761601901384092E-6 20-21 0.0016849911938249156 5.4092815211072736E-6 2.7046407605536368E-6 5.274049483079591E-5 8.11392228166091E-6 22-23 0.0024815078978079616 5.4092815211072736E-6 2.7046407605536368E-6 5.544513559134956E-5 1.0818563042214547E-5 24-25 0.003616104696860212 5.4092815211072736E-6 2.7046407605536368E-6 6.355905787301047E-5 1.0818563042214547E-5 26-27 0.005054973581474748 5.4092815211072736E-6 2.7046407605536368E-6 7.437762091522501E-5 1.0818563042214547E-5 28-29 0.006307222253611081 5.4092815211072736E-6 2.7046407605536368E-6 7.708226167577864E-5 1.0818563042214547E-5 30-31 0.007305234694255373 8.11392228166091E-6 2.7046407605536368E-6 8.249154319688592E-5 1.0818563042214547E-5 32-33 0.008039544660745686 8.11392228166091E-6 2.7046407605536368E-6 9.736706737993093E-5 1.0818563042214547E-5 34-35 0.008765740704954336 8.11392228166091E-6 2.7046407605536368E-6 1.0412866928131501E-4 1.0818563042214547E-5 36-37 0.009573075971979598 8.11392228166091E-6 2.7046407605536368E-6 1.0548098966159183E-4 1.0818563042214547E-5 38-39 0.010287101132765757 8.11392228166091E-6 2.7046407605536368E-6 1.0953795080242229E-4 1.2170883422491366E-5 40-41 0.010968670604425274 8.11392228166091E-6 2.7046407605536368E-6 1.176518730840832E-4 1.3523203802768184E-5 42-43 0.011778710512211087 8.11392228166091E-6 2.7046407605536368E-6 1.2306115460519049E-4 1.4875524183045001E-5 44-45 0.012939001398488598 8.11392228166091E-6 2.7046407605536368E-6 1.2982275650657457E-4 1.622784456332182E-5 46-47 0.014717302698552615 8.11392228166091E-6 2.7046407605536368E-6 1.3523203802768183E-4 1.622784456332182E-5 48-49 0.01734215655666992 8.11392228166091E-6 2.7046407605536368E-6 1.3523203802768183E-4 1.622784456332182E-5 50-51 0.02107320848585366 8.11392228166091E-6 2.7046407605536368E-6 1.392889991685123E-4 2.0284805704152275E-5 52-53 0.025986188427399342 8.11392228166091E-6 2.7046407605536368E-6 1.5416452335155728E-4 2.1637126084429094E-5 54-55 0.03315754340400731 8.11392228166091E-6 2.7046407605536368E-6 1.5957380487266457E-4 2.1637126084429094E-5 56-57 0.04303489146154919 8.11392228166091E-6 2.7046407605536368E-6 1.690400475346023E-4 2.1637126084429094E-5 58-59 0.056261937101036746 9.466242661937728E-6 2.7046407605536368E-6 1.7039236791487912E-4 2.1637126084429094E-5 60-61 0.07316999881563782 1.4875524183045001E-5 4.056961140830455E-6 1.8391557171764728E-4 2.1637126084429094E-5 62-63 0.0946380848525323 1.622784456332182E-5 5.4092815211072736E-6 1.906771736190314E-4 2.1637126084429094E-5 64-65 0.12168990173958977 1.622784456332182E-5 5.4092815211072736E-6 2.0284805704152276E-4 2.1637126084429094E-5 66-67 0.1555587656636227 1.622784456332182E-5 5.4092815211072736E-6 2.0960965894290684E-4 2.1637126084429094E-5 68-69 0.19673015964115043 1.758016494359864E-5 5.4092815211072736E-6 2.1772358122456776E-4 2.1637126084429094E-5 70-71 0.24795470332565603 1.8932485323875456E-5 5.4092815211072736E-6 2.217805423653982E-4 2.434176684498273E-5 72-73 0.3093432869883222 1.8932485323875456E-5 5.4092815211072736E-6 2.2583750350622868E-4 2.434176684498273E-5 74-75 0.382601186628678 1.8932485323875456E-5 5.4092815211072736E-6 2.3259910540761276E-4 2.434176684498273E-5 76-77 0.4704546801333615 2.0284805704152275E-5 5.4092815211072736E-6 2.366560665484432E-4 2.434176684498273E-5 78-79 0.5740816385535938 2.298944646470591E-5 5.4092815211072736E-6 2.4071302768927366E-4 2.434176684498273E-5 80-81 0.6953360931307344 2.434176684498273E-5 5.4092815211072736E-6 2.4206534806955047E-4 2.7046407605536368E-5 82-83 0.8355081575075676 2.434176684498273E-5 5.4092815211072736E-6 2.5288391111176505E-4 2.9751048366090003E-5 84-85 0.9975648225984203 2.434176684498273E-5 5.4092815211072736E-6 2.6235015377370276E-4 3.110336874636682E-5 86-87 1.1821105756132766 2.434176684498273E-5 5.4092815211072736E-6 2.6235015377370276E-4 3.245568912664364E-5 88 1.334726692129417 2.434176684498273E-5 5.4092815211072736E-6 2.650547945342564E-4 3.245568912664364E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ATCTCGA 27555 0.0 46.87296 1 TCTCGAG 33480 0.0 38.083782 2 CTCGAGG 41510 0.0 30.297129 3 TGATACG 4390 0.0 28.369358 3 TCGAGGG 47825 0.0 26.149176 4 ATACGGC 5040 0.0 24.337685 5 GATCCGG 40485 0.0 22.786043 1 TATCTCG 17575 0.0 19.975653 2 ATCCGGC 47005 0.0 19.626913 2 AGGGCGC 70200 0.0 17.748005 7 ACGACGC 3405 0.0 17.736044 40-41 CGAGGGC 71760 0.0 17.597643 5 ATATCTC 20690 0.0 17.45181 1 GGGCGCG 74765 0.0 16.777521 8 TCCGGCG 56475 0.0 16.310524 3 TATGGAT 23815 0.0 15.708628 2 GCGCCGT 58910 0.0 15.126115 9 CGGCGCG 60320 0.0 15.09947 5 GAGGGCG 85435 0.0 14.8359585 6 GGATCCG 28875 0.0 14.567224 2 >>END_MODULE