FastQCFastQC Report
Wed 25 May 2016
SRR491033_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR491033_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29963595
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCTCGA232200.042.4084931
TCTCGAG260100.037.6017042
CTCGAGG347900.027.8818533
TATCTCG109800.027.5650832
TCGAGGG391600.024.4583914
GATCCGG291150.021.6628671
TGATACG33650.021.2287733
ATATCTC148950.021.0454271
TACGGCG34550.020.1318176
ATACGGC39200.019.1823795
ATCCGGC334950.018.7737882
CGAGGGC517200.018.5279455
TATGGAT175800.017.5639552
AGGGCGC558600.017.0881757
TCCGGCG369600.017.0006623
GAGGGCG597800.016.0613386
GGGCGCG609100.015.8643638
ATATGGA206000.015.4001321
GGATCCG207000.014.53066352
GATACGG49050.014.3720464
Produced by FastQC (version 0.11.5)