##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR491033_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 29963595 Sequences flagged as poor quality 0 Sequence length 100 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.88775699311114 31.0 30.0 34.0 26.0 34.0 2 30.229499864752544 31.0 30.0 34.0 26.0 34.0 3 30.345703644706184 31.0 30.0 34.0 26.0 34.0 4 33.81698414359158 37.0 35.0 37.0 28.0 37.0 5 33.35324529650064 37.0 35.0 37.0 27.0 37.0 6 33.23626177032496 36.0 35.0 37.0 27.0 37.0 7 33.16502325572082 36.0 35.0 37.0 26.0 37.0 8 33.107265800382095 36.0 35.0 37.0 26.0 37.0 9 34.438890793978494 38.0 34.0 39.0 27.0 39.0 10-11 34.37554727662018 38.0 34.0 39.0 27.0 39.0 12-13 34.31432278069437 38.0 34.0 39.0 26.5 39.0 14-15 35.31044756144915 39.0 34.5 41.0 26.0 41.0 16-17 35.247610275068794 39.0 34.5 41.0 26.0 41.0 18-19 35.202371527849046 39.0 34.0 41.0 25.0 41.0 20-21 35.13997434219759 39.0 34.0 41.0 25.0 41.0 22-23 34.99005888645872 39.0 34.0 41.0 25.0 41.0 24-25 34.89116576298672 39.0 34.0 41.0 24.0 41.0 26-27 34.70417147875614 39.0 34.0 41.0 23.5 41.0 28-29 34.48810022629127 38.5 34.0 41.0 23.0 41.0 30-31 34.30745499663842 38.0 33.5 40.0 21.5 41.0 32-33 34.074795213992175 38.0 33.0 40.0 19.0 41.0 34-35 33.87870981435972 38.0 33.0 40.0 18.0 41.0 36-37 33.60135634592578 38.0 33.0 40.0 16.5 41.0 38-39 33.44161808688176 38.0 33.0 40.0 15.5 41.0 40-41 33.24391393956566 38.0 32.5 40.0 14.0 41.0 42-43 32.92651527628777 38.0 32.0 40.0 9.0 41.0 44-45 32.592413126662535 38.0 31.5 40.0 7.5 41.0 46-47 32.476454110396304 38.0 31.0 40.0 2.0 41.0 48-49 32.276446033928835 38.0 31.0 40.0 2.0 41.0 50-51 32.05493808403164 37.0 31.0 40.0 2.0 41.0 52-53 31.807928087400725 37.0 30.5 40.0 2.0 41.0 54-55 31.549642774840603 37.0 30.0 40.0 2.0 41.0 56-57 31.274871823624636 36.0 30.0 40.0 2.0 41.0 58-59 30.991274995540422 36.0 29.0 40.0 2.0 41.0 60-61 30.7030392881762 35.5 29.0 39.0 2.0 41.0 62-63 30.47550666066605 35.0 29.0 39.0 2.0 41.0 64-65 30.430211261365667 35.0 29.0 39.0 2.0 41.0 66-67 30.17931710130243 35.0 29.0 39.0 2.0 41.0 68-69 29.835813459633265 35.0 28.5 38.5 2.0 40.5 70-71 29.504068570543687 35.0 28.0 38.0 2.0 40.0 72-73 29.152624910328683 34.5 28.0 37.0 2.0 40.0 74-75 28.798167843344565 34.0 27.5 36.5 2.0 39.0 76-77 27.980563363641778 33.5 26.0 36.0 2.0 39.0 78-79 28.099626146328568 34.0 26.5 36.0 2.0 38.5 80-81 27.909783789294977 34.0 26.0 35.0 2.0 37.0 82-83 27.59420877234524 34.0 26.0 35.0 2.0 37.0 84-85 27.254446020245567 34.0 26.0 35.0 2.0 36.5 86-87 26.984499256514447 34.0 25.0 35.0 2.0 36.0 88-89 26.72476765555001 34.0 25.0 35.0 2.0 36.0 90-91 26.45094110035862 33.5 24.5 35.0 2.0 35.5 92-93 26.16863749159605 33.0 24.0 35.0 2.0 35.0 94-95 25.893054488288204 33.0 23.0 35.0 2.0 35.0 96-97 25.556444829133486 33.0 19.5 35.0 2.0 35.0 98-99 25.214985902058814 33.0 16.5 35.0 2.0 35.0 100 24.487512696657394 32.0 2.0 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.525110353525271 1101 2 0.4954877173199712 1101 3 0.48985259115987745 1101 4 0.5475235290404541 1101 5 0.6459974702555229 1101 6 0.7373844338853672 1101 7 0.7447441798184968 1101 8 0.7114984084739859 1101 9 0.7952319436651223 1101 10-11 0.8270418334265415 1101 12-13 0.7982923890418476 1101 14-15 0.8671409305236182 1101 16-17 0.78637517589857 1101 18-19 0.7431513740519762 1101 20-21 0.8100982005247346 1101 22-23 0.7866764706954896 1101 24-25 0.8658153826880195 1101 26-27 0.8574208186109544 1101 28-29 0.8021505419004242 1101 30-31 0.8018455446137907 1101 32-33 0.8241979941405333 1101 34-35 0.843078068814016 1101 36-37 0.8685717086990934 1101 38-39 0.7496750684449367 1101 40-41 0.7474426221474388 1101 42-43 0.7755884323626603 1101 44-45 0.6507646569176728 1101 46-47 0.5821732496327527 1101 48-49 0.6045534803715853 1101 50-51 0.5459221201490223 1101 52-53 0.6731074584667347 1101 54-55 0.4467336961472803 1101 56-57 0.4719464403571507 1101 58-59 0.4447358600027691 1101 60-61 0.5169544340015442 1101 62-63 0.5562894376523673 1101 64-65 0.5278741658641799 1101 66-67 0.6007959803288507 1101 68-69 0.6094300417510574 1101 70-71 0.6666493653232344 1101 72-73 0.6168058568330466 1101 74-75 0.5685853679264241 1101 76-77 0.5867300054387385 1101 78-79 0.6269865682553721 1101 80-81 0.5922567587862702 1101 82-83 0.49180270348321997 1101 84-85 0.4355109118801259 1101 86-87 0.37429819138865383 1101 88-89 0.4277031231955597 1101 90-91 0.32373728235795696 1101 92-93 0.26215876039760744 1101 94-95 0.18644436853118407 1101 96-97 0.18384543144546583 1101 98-99 0.15713445474947818 1101 100 0.14517149319899403 1102 1 0.2281099322188247 1102 2 0.17936580878727781 1102 3 0.2097389634504303 1102 4 0.3064675792621898 1102 5 0.4034190405250655 1102 6 0.38904265567477125 1102 7 0.3691378703949795 1102 8 0.28591823190696886 1102 9 0.2600160526204718 1102 10-11 0.32711699565292207 1102 12-13 0.37855322009829706 1102 14-15 0.3101607723066522 1102 16-17 0.19591760986495643 1102 18-19 0.32394114808824526 1102 20-21 0.40304812475449125 1102 22-23 0.3670271325774408 1102 24-25 0.37274387707680745 1102 26-27 0.38018377562675454 1102 28-29 0.3845935154465323 1102 30-31 0.32232968906676973 1102 32-33 0.31829352775041997 1102 34-35 0.2850394621886423 1102 36-37 0.4418370292732945 1102 38-39 0.4176638701676296 1102 40-41 0.28283774120801297 1102 42-43 0.23785601075488927 1102 44-45 0.2979133166061487 1102 46-47 0.20698921381126212 1102 48-49 -0.020107225644096616 1102 50-51 0.018517127054728633 1102 52-53 0.16160280626552392 1102 54-55 0.25028706625752406 1102 56-57 0.12591296884373193 1102 58-59 -0.06457907248954342 1102 60-61 0.0026337191480436672 1102 62-63 0.08254175051279589 1102 64-65 0.15361830044806268 1102 66-67 0.039163846529348234 1102 68-69 0.12328459680637494 1102 70-71 0.012049806571400978 1102 72-73 0.11332367528100207 1102 74-75 -0.025566781000232908 1102 76-77 0.015342230801799417 1102 78-79 0.028848651215451326 1102 80-81 0.07060959506281606 1102 82-83 0.04677306805785264 1102 84-85 0.0462881077195334 1102 86-87 -0.11048833629033084 1102 88-89 0.013611480308842516 1102 90-91 0.08023891369211 1102 92-93 -0.010689905243772557 1102 94-95 0.03901565929989559 1102 96-97 -0.055458783133104816 1102 98-99 -0.008191509425408583 1102 100 -0.021130126764642654 1103 1 -0.031089013156780254 1103 2 -0.049433258826436344 1103 3 -0.014297248599927315 1103 4 0.00508488880189617 1103 5 0.044435034975229826 1103 6 0.03692342122221248 1103 7 0.045865564242603796 1103 8 0.019092844791238406 1103 9 -0.07749816176257696 1103 10-11 -0.08709000123456434 1103 12-13 0.0325459622286175 1103 14-15 -0.07248614824455046 1103 16-17 0.0017803765565744811 1103 18-19 -0.07139207320408048 1103 20-21 -0.0013996812266015013 1103 22-23 -0.02669819184129807 1103 24-25 0.05677361547461146 1103 26-27 0.030549385061597434 1103 28-29 0.03461564207450607 1103 30-31 -0.02218794937195412 1103 32-33 -0.0011768655400032912 1103 34-35 0.03575123224054266 1103 36-37 0.12439120539476534 1103 38-39 0.052209443930330224 1103 40-41 -0.04442724330660042 1103 42-43 -0.05741591793834999 1103 44-45 -0.04965996985269783 1103 46-47 -0.01217390720598388 1103 48-49 -0.13275289389790856 1103 50-51 -0.15202830608546947 1103 52-53 -0.08535561718120377 1103 54-55 -0.07085748322299779 1103 56-57 -0.15992652907423377 1103 58-59 -0.05053947755326149 1103 60-61 -0.04432398643933766 1103 62-63 -0.0034805614517665617 1103 64-65 0.0406842002069574 1103 66-67 0.07450021804024587 1103 68-69 0.09336611207262635 1103 70-71 0.1178492989825024 1103 72-73 -0.0033491461178414283 1103 74-75 0.04763073582329369 1103 76-77 0.07360415722511959 1103 78-79 0.06943415530910713 1103 80-81 0.10123980241579744 1103 82-83 0.0871591586192082 1103 84-85 0.06483204163371781 1103 86-87 0.0518860206217866 1103 88-89 0.08940259611069123 1103 90-91 -0.016708455095404418 1103 92-93 0.0349124961422973 1103 94-95 -0.02829626395397966 1103 96-97 -0.08152339802419206 1103 98-99 -0.21296604901129612 1103 100 -0.15606369615850113 1104 1 -0.292836658819823 1104 2 -0.24581575671797395 1104 3 -0.2954012811912996 1104 4 -0.17356683589792965 1104 5 -0.2562319580991641 1104 6 -0.29437213034623966 1104 7 -0.29756692462513 1104 8 -0.17346928069140688 1104 9 -0.32718766500472185 1104 10-11 -0.3759822261822663 1104 12-13 -0.34580882382088163 1104 14-15 -0.3764716514777149 1104 16-17 -0.3165507975532833 1104 18-19 -0.39338718837606024 1104 20-21 -0.35180989886522696 1104 22-23 -0.3007111403744105 1104 24-25 -0.3146853505759779 1104 26-27 -0.34400049635194563 1104 28-29 -0.3559277267411929 1104 30-31 -0.23154433764975835 1104 32-33 -0.2733714447106834 1104 34-35 -0.2962584391142684 1104 36-37 -0.2837075290111599 1104 38-39 -0.3577121805603767 1104 40-41 -0.41506547456423704 1104 42-43 -0.43192409215874505 1104 44-45 -0.3972041641060997 1104 46-47 -0.4264856211109489 1104 48-49 -0.39146786834838565 1104 50-51 -0.31041183165915953 1104 52-53 -0.384936200969225 1104 54-55 -0.5315239799254314 1104 56-57 -0.3170938934673124 1104 58-59 -0.2818090886075737 1104 60-61 -0.29462183573208733 1104 62-63 -0.43484563114192554 1104 64-65 -0.37551405980404695 1104 66-67 -0.3371885206391134 1104 68-69 -0.3540790546279844 1104 70-71 -0.38557045241300614 1104 72-73 -0.3084161732535051 1104 74-75 -0.3851147731290432 1104 76-77 -0.3660189612329283 1104 78-79 -0.3871877995408646 1104 80-81 -0.39511783223716535 1104 82-83 -0.45810669107939717 1104 84-85 -0.47871645950738184 1104 86-87 -0.47698122944993315 1104 88-89 -0.47215273199145 1104 90-91 -0.42837824038067396 1104 92-93 -0.4720873806904997 1104 94-95 -0.46042231853383697 1104 96-97 -0.7270743099066763 1104 98-99 -0.6988716823034267 1104 100 -0.6620053042974732 1105 1 -0.6700386918868091 1105 2 -0.6595034672424767 1105 3 -0.6512805892497937 1105 4 -0.5560503861345936 1105 5 -0.6137659538434264 1105 6 -0.8227282612741647 1105 7 -0.7351382484139108 1105 8 -0.7497403822687971 1105 9 -0.8737955013241532 1105 10-11 -0.8348886688904145 1105 12-13 -0.9561239620781166 1105 14-15 -1.0700149415539855 1105 16-17 -1.0049453833568691 1105 18-19 -0.9695580060942035 1105 20-21 -0.9623107346927426 1105 22-23 -0.9525320301314295 1105 24-25 -0.9266277675323593 1105 26-27 -1.0232544698839874 1105 28-29 -1.050902717427192 1105 30-31 -0.9770675829239934 1105 32-33 -1.0151021756517409 1105 34-35 -0.9607705969036147 1105 36-37 -0.8436454758798035 1105 38-39 -0.9533031701078514 1105 40-41 -1.0220698182036756 1105 42-43 -0.9435112689262084 1105 44-45 -0.8710677172775405 1105 46-47 -0.8422037533803106 1105 48-49 -0.8843409391391965 1105 50-51 -0.8519923989659972 1105 52-53 -0.9147860547390287 1105 54-55 -0.8682639889846904 1105 56-57 -0.8851862619746349 1105 58-59 -0.8573705823242008 1105 60-61 -0.8075820065234396 1105 62-63 -0.8658306660421253 1105 64-65 -0.8972944566126628 1105 66-67 -0.8841106660548128 1105 68-69 -0.8151674688533639 1105 70-71 -0.7798997788351407 1105 72-73 -0.7528602274639056 1105 74-75 -0.8081965895429697 1105 76-77 -0.801019795795284 1105 78-79 -0.7074598691404006 1105 80-81 -0.6339104501076278 1105 82-83 -0.6548411342299296 1105 84-85 -0.6152711156668218 1105 86-87 -0.6579695426512124 1105 88-89 -0.7368069147489962 1105 90-91 -0.8308981705687124 1105 92-93 -0.6952964125165657 1105 94-95 -0.6891400507597503 1105 96-97 -0.662568077044206 1105 98-99 -0.6496520366382263 1105 100 -0.7091873112347891 1106 1 -1.0632585590294141 1106 2 -1.0725106059565839 1106 3 -1.0820154856071795 1106 4 -1.0811933877816813 1106 5 -1.1685283016121417 1106 6 -1.162097474147238 1106 7 -1.2528190632935932 1106 8 -1.2278118344027433 1106 9 -1.4781765204617514 1106 10-11 -1.4646861345633866 1106 12-13 -1.5301972726486426 1106 14-15 -1.7422487059077696 1106 16-17 -1.7264440196675466 1106 18-19 -1.7993842818971046 1106 20-21 -1.7104812251070598 1106 22-23 -1.8292051567149699 1106 24-25 -1.7648542496617878 1106 26-27 -1.8293602723522895 1106 28-29 -1.8167079774968045 1106 30-31 -1.761823759440368 1106 32-33 -1.7464941560625746 1106 34-35 -1.7714465628632254 1106 36-37 -1.6266053790440225 1106 38-39 -1.8012006753519287 1106 40-41 -1.804747735630123 1106 42-43 -1.8557269949318709 1106 44-45 -1.8404413356921765 1106 46-47 -1.8561651302394893 1106 48-49 -1.73720343624462 1106 50-51 -1.7626366755274034 1106 52-53 -1.8079400125282135 1106 54-55 -1.7691369864251563 1106 56-57 -1.8509938237960952 1106 58-59 -1.8154570199552111 1106 60-61 -1.8308394047856282 1106 62-63 -1.7480842404032728 1106 64-65 -1.6980919862878459 1106 66-67 -1.8820941753429459 1106 68-69 -1.7725334575689224 1106 70-71 -1.68549139169588 1106 72-73 -1.7096168590800787 1106 74-75 -1.6669849059859239 1106 76-77 -1.6722532281848572 1106 78-79 -1.5671654198209062 1106 80-81 -1.579530291858248 1106 82-83 -1.545083438454288 1106 84-85 -1.6352842077484588 1106 86-87 -1.6086359465924076 1106 88-89 -1.564041649815131 1106 90-91 -1.5467691611864787 1106 92-93 -1.5788898364322428 1106 94-95 -1.6235277203993128 1106 96-97 -1.6110002519487026 1106 98-99 -1.6278273089399704 1106 100 -1.6130607311498224 1107 1 -1.6696223427374406 1107 2 -1.6129814597828478 1107 3 -1.6287084625563004 1107 4 -1.6505239600463497 1107 5 -1.9949241801958166 1107 6 -1.9969387912828722 1107 7 -2.0835151141965547 1107 8 -2.032616898443475 1107 9 -2.2546460399827453 1107 10-11 -2.318411189615766 1107 12-13 -2.3317792164748354 1107 14-15 -2.6231539641314896 1107 16-17 -2.6808226634243013 1107 18-19 -2.736414095651199 1107 20-21 -2.701617583685035 1107 22-23 -2.7617018560436293 1107 24-25 -2.729047978877851 1107 26-27 -2.6523870847881668 1107 28-29 -2.6625117952944457 1107 30-31 -2.7392101472963617 1107 32-33 -2.934150021965337 1107 34-35 -2.828597106310653 1107 36-37 -2.671283543849185 1107 38-39 -2.892592337715598 1107 40-41 -3.015326924582464 1107 42-43 -2.8565624183427474 1107 44-45 -2.8863967538087216 1107 46-47 -2.822172759760033 1107 48-49 -2.8314761749471664 1107 50-51 -2.7823107630724593 1107 52-53 -2.737322877804754 1107 54-55 -2.7063647941688167 1107 56-57 -2.814713247421242 1107 58-59 -2.7941309023502505 1107 60-61 -2.7839387936279607 1107 62-63 -2.820920108551782 1107 64-65 -2.970021014568001 1107 66-67 -2.8298018996093326 1107 68-69 -2.782014175262841 1107 70-71 -2.8503717544287532 1107 72-73 -2.7025706096624944 1107 74-75 -2.902507097686449 1107 76-77 -2.9746070362789467 1107 78-79 -2.9142309996175655 1107 80-81 -2.8107128837059605 1107 82-83 -2.9393521907737252 1107 84-85 -2.843518108064327 1107 86-87 -2.9337587634220164 1107 88-89 -2.782482946047189 1107 90-91 -2.7481756316961814 1107 92-93 -2.745815504308542 1107 94-95 -2.8176414036802804 1107 96-97 -2.866905085255091 1107 98-99 -2.974397845047264 1107 100 -2.9434760177533086 1108 1 -2.367659923350786 1108 2 -2.479736985216128 1108 3 -2.3446961821416252 1108 4 -2.462379420613896 1108 5 -2.959527797428265 1108 6 -2.9766839883729155 1108 7 -3.065042850357454 1108 8 -3.16850156439439 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-0.014571573351730649 2306 6 0.03310950555772507 2306 7 -0.34062283679994465 2306 8 -0.14756584219949076 2306 9 -0.012750484265843909 2306 10-11 0.06872307083799711 2306 12-13 0.05413901055904091 2306 14-15 0.018802801949107106 2306 16-17 0.00994814614216466 2306 18-19 0.04099998429451546 2306 20-21 0.11145734391016049 2306 22-23 0.13618502216389317 2306 24-25 0.06910281158114628 2306 26-27 0.12803141342671864 2306 28-29 0.09473812941002535 2306 30-31 0.05629059963406746 2306 32-33 0.17153706534983115 2306 34-35 0.22719845718090426 2306 36-37 0.3461130056427848 2306 38-39 0.30955576332298307 2306 40-41 0.22210317388766043 2306 42-43 0.22816749128919156 2306 44-45 0.20015217515539518 2306 46-47 0.11516560862413172 2306 48-49 0.19532726577058668 2306 50-51 0.17084801193470156 2306 52-53 0.20382952656747833 2306 54-55 0.32792345723536087 2306 56-57 0.3402539009739165 2306 58-59 0.33784080437948205 2306 60-61 0.2983597039899166 2306 62-63 0.34097676752953276 2306 64-65 0.31777057077870907 2306 66-67 0.28916270450735837 2306 68-69 0.3477170099352769 2306 70-71 0.37671690453277407 2306 72-73 0.40773405273368013 2306 74-75 0.39573314325052067 2306 76-77 0.3831835553788565 2306 78-79 0.36676751759638293 2306 80-81 0.3198975663020214 2306 82-83 0.413154568937415 2306 84-85 0.39723969602050957 2306 86-87 0.36512218835433785 2306 88-89 0.33520066776118895 2306 90-91 0.40874683462661565 2306 92-93 0.4069763923879641 2306 94-95 0.49383046427975685 2306 96-97 0.5780153507590455 2306 98-99 0.5456015166059132 2306 100 0.5262469793283806 2307 1 -0.5378845651030488 2307 2 -0.507384724601657 2307 3 -0.5258071642629254 2307 4 -0.5101007665101847 2307 5 -0.5547087534996464 2307 6 -0.5350272867605881 2307 7 -0.56521412275724 2307 8 -0.6307366452928562 2307 9 -0.9620791201284575 2307 10-11 -0.6997118283731325 2307 12-13 -0.7043780049941759 2307 14-15 -0.7644853877608426 2307 16-17 -0.7939006237282271 2307 18-19 -0.8722860995374191 2307 20-21 -0.8743677849331704 2307 22-23 -0.7408250676126968 2307 24-25 -0.810192029834937 2307 26-27 -0.7801561983905714 2307 28-29 -0.7728960228678545 2307 30-31 -0.7567506382301019 2307 32-33 -0.773479072134954 2307 34-35 -0.9881538292680645 2307 36-37 -0.8394042692243531 2307 38-39 -0.7990703651315059 2307 40-41 -0.7221564272876577 2307 42-43 -0.7565134089404637 2307 44-45 -0.703262045960102 2307 46-47 -0.6636048680811655 2307 48-49 -0.634813868558517 2307 50-51 -0.6798919068654712 2307 52-53 -0.6720623380684891 2307 54-55 -0.7075053015635255 2307 56-57 -0.8150665461945188 2307 58-59 -0.7405886236620169 2307 60-61 -0.6281253963747062 2307 62-63 -0.6247295191205708 2307 64-65 -0.916903508223097 2307 66-67 -0.7357231147601873 2307 68-69 -0.708077724163207 2307 70-71 -0.7089443529462152 2307 72-73 -0.7562194981471144 2307 74-75 -0.8257450141210114 2307 76-77 -0.8582453097508065 2307 78-79 -0.8032506654701557 2307 80-81 -0.8490435564003143 2307 82-83 -0.8274645887728234 2307 84-85 -0.9143611615317546 2307 86-87 -0.8364332455268695 2307 88-89 -0.8303052755870048 2307 90-91 -0.9079701175777899 2307 92-93 -0.8857704276384197 2307 94-95 -0.8274763054336987 2307 96-97 -0.7290036989888655 2307 98-99 -0.7645973244097668 2307 100 -0.7955877866005743 2308 1 -1.6055359994909608 2308 2 -1.5688802522983814 2308 3 -1.508477878021118 2308 4 -1.4754508600027165 2308 5 -1.8007373864257161 2308 6 -1.7349125185444052 2308 7 -1.851631354975737 2308 8 -1.8855949839097228 2308 9 -2.0222477752028993 2308 10-11 -2.166099919539576 2308 12-13 -2.1861724520747785 2308 14-15 -2.474260162481535 2308 16-17 -2.437750601534482 2308 18-19 -2.483870154905425 2308 20-21 -2.6186325965413033 2308 22-23 -2.6704913543123183 2308 24-25 -2.673707544003925 2308 26-27 -2.5220171118612953 2308 28-29 -2.457599772286599 2308 30-31 -2.3870138581362284 2308 32-33 -2.4242092092406295 2308 34-35 -2.418611439461664 2308 36-37 -2.6817980415825957 2308 38-39 -2.5322040390363583 2308 40-41 -2.591844681762801 2308 42-43 -2.7646800110406673 2308 44-45 -2.8151965418453813 2308 46-47 -2.45629090539191 2308 48-49 -2.546352907282767 2308 50-51 -2.376777955312992 2308 52-53 -2.624017511963192 2308 54-55 -2.536184716484083 2308 56-57 -2.5134345683855237 2308 58-59 -2.425384535649652 2308 60-61 -2.4083956839808565 2308 62-63 -2.687125490661245 2308 64-65 -2.753620541570232 2308 66-67 -3.0344158873158484 2308 68-69 -2.835703188184599 2308 70-71 -2.7062235778948285 2308 72-73 -2.556139675960214 2308 74-75 -2.6678488500779878 2308 76-77 -2.617077636080591 2308 78-79 -2.544011209919539 2308 80-81 -2.5000232381906144 2308 82-83 -2.3505861243760187 2308 84-85 -2.3918076839917894 2308 86-87 -2.613567149133811 2308 88-89 -2.6981586315499335 2308 90-91 -2.4665728399459574 2308 92-93 -2.3556425134308654 2308 94-95 -2.216859256420687 2308 96-97 -2.68624235247367 2308 98-99 -2.4992813595134784 2308 100 -2.4435391974720027 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 970618.0 3 271025.0 4 142612.0 5 114748.0 6 113150.0 7 123412.0 8 138498.0 9 151289.0 10 160300.0 11 160762.0 12 166971.0 13 192393.0 14 239818.0 15 297095.0 16 331466.0 17 304393.0 18 249543.0 19 205985.0 20 195409.0 21 201439.0 22 218625.0 23 251897.0 24 295428.0 25 352518.0 26 421161.0 27 500433.0 28 589868.0 29 691696.0 30 816474.0 31 966591.0 32 1164455.0 33 1428843.0 34 1806356.0 35 2375567.0 36 3268174.0 37 4431638.0 38 4379742.0 39 1270209.0 40 2994.0 >>END_MODULE >>Per base sequence content pass #Base G A T C 1 20.95762742872768 28.1759376002518 28.33590528065415 22.53052969036638 2 23.61883135163296 27.471098012692696 27.405864017524333 21.50420661815001 3 23.404374541839857 26.390574962717256 26.937642162097035 23.26740833334585 4 24.08947106945186 26.107675340392273 26.874063986745483 22.928789603410387 5 24.550986295111084 26.085141553552262 26.490392694152316 22.873479457184338 6 24.563439343756084 25.962131579198562 25.908013523940138 23.566415553105216 7 24.419247964831257 26.45365238292573 26.083386246296534 23.043713405946473 8 24.10066179489745 26.10861943479048 26.302630631212775 23.48808813909929 9 23.929040153853563 25.874048499359336 26.424441005316446 23.77247034147065 10-11 23.80193805998847 26.076693086453677 26.409614878411443 23.711753975146408 12-13 23.54684812145644 26.146050300048774 26.56363107623279 23.743470502261996 14-15 23.306279845719153 26.319106186841427 26.974880897498082 23.399733069941338 16-17 23.100543234218083 26.247496093797007 27.230404399721674 23.42155627226324 18-19 22.760056827938946 26.196952232122072 27.852702623093684 23.190288316845297 20-21 22.46471919703872 25.94522172388646 28.582443148116166 23.007615930958654 22-23 22.40969597493725 25.931139618402995 28.94336180452229 22.715802602137465 24-25 22.37329741885042 25.807288577087988 29.286122216639114 22.533291787422474 26-27 22.442655768726738 25.841278333960965 29.31265526222486 22.40341063508743 28-29 22.428754195932576 25.938175052223496 29.277576559482398 22.35549419236153 30-31 22.432715093862075 25.860825542465278 29.247334406149555 22.459124957523095 32-33 22.46992526124519 25.897647758571658 29.193143874105655 22.439283106077497 34-35 22.524082359660703 25.97464114276773 29.135341118976086 22.36593537859548 36-37 22.686354420148735 25.876306714175495 29.000086378878585 22.43725248679718 38-39 22.578115685792053 25.969483675883687 28.962214413242197 22.490186225082063 40-41 22.663908520713765 26.100466390878772 28.74381364711499 22.491811441292473 42-43 22.777460431253317 26.181213986549135 28.3543707961865 22.68695478601105 44-45 22.89047035826879 26.35227731090053 27.978908166430998 22.778344164399684 46-47 23.025891866522294 26.569473873998717 27.567660606264504 22.836973653214482 48-49 23.137280660232246 26.611971058300682 27.19799352646145 23.052754755005626 50-51 23.189173097632967 26.67684300266316 27.0172041778726 23.11677972183128 52-53 23.255819930042158 26.7666301404537 26.849069442658852 23.12848048684529 54-55 23.272724341766274 26.755066463052664 26.761631390915134 23.210577804265924 56-57 23.256476932658142 26.740800420724415 26.78564774232852 23.217074904288918 58-59 23.28144262016695 26.830691771152992 26.75960629355711 23.128259315122957 60-61 23.259594726526647 26.81753248461027 26.698534165795902 23.22433862306718 62-63 23.232591652441183 26.891281602406934 26.710070867249957 23.166055877901922 64-65 23.251005004773752 27.05033539430052 26.63402907732934 23.06463052359638 66-67 23.254296403971463 27.048765565581228 26.555396646115987 23.141541384331326 68-69 23.291356613148647 27.12150933894872 26.4755053027314 23.11162874517123 70-71 23.297822992031357 27.278521176748022 26.39872084871969 23.024934982500934 72-73 23.2983624756591 27.31660759200554 26.278247286119296 23.106782646216065 74-75 23.30107466070805 27.400404576617582 26.255190520489595 23.043330242184766 76-77 23.18063481016752 27.61313655887357 26.137159564696184 23.069069066262728 78-79 23.187997639504932 27.65412185865558 26.054591754967742 23.103288746871748 80-81 23.14531793525684 27.742168840906395 26.0486398872189 23.063873336617867 82-83 23.141468005673214 27.879635945891273 25.960175446323507 23.018720602112005 84-85 23.14042739777533 27.954647384351304 25.84185887095859 23.063066346914777 86-87 23.142344198402913 28.026982621580743 25.744170472154654 23.086502707861687 88-89 23.1763045109588 28.148458619610174 25.68555455889099 22.98968231054004 90-91 23.183164570624456 28.217770943862792 25.58278085709638 23.016283628416375 92-93 23.207084154599062 28.29117103485498 25.478118874403993 23.02362593614197 94-95 23.21229563762609 28.44750577312943 25.346846665842538 22.99335192340194 96-97 23.183099593258344 28.532898034673003 25.229668786973775 23.054333585094874 98-99 23.201243251291896 28.619551891739192 25.114219432170948 23.064985424797964 100 23.238134794743598 28.785230920732058 24.771065540002155 23.20556874452219 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 3376.0 1 2315.0 2 1206.5 3 1168.0 4 1235.0 5 1406.0 6 1660.5 7 1928.0 8 2185.0 9 2583.5 10 3270.0 11 4133.5 12 5189.5 13 7343.5 14 12944.5 15 18048.5 16 25526.0 17 29337.0 18 29116.0 19 35368.5 20 45541.0 21 58718.0 22 75346.5 23 96418.5 24 120308.0 25 148238.0 26 182947.5 27 228074.5 28 279827.0 29 328627.0 30 375817.5 31 426520.5 32 480834.5 33 538016.0 34 593815.0 35 653422.5 36 715497.0 37 778928.5 38 840361.5 39 889040.0 40 928464.5 41 964310.5 42 994814.5 43 1016566.5 44 1024049.5 45 1021589.5 46 1022096.0 47 1018632.5 48 1010130.5 49 996087.5 50 979468.0 51 965112.5 52 931242.5 53 886109.5 54 846749.0 55 807632.0 56 760521.5 57 698337.5 58 642985.0 59 602077.5 60 552273.5 61 501194.0 62 453324.5 63 400144.5 64 348531.0 65 297741.5 66 250392.5 67 208915.0 68 172597.5 69 140159.0 70 110131.5 71 86272.5 72 69335.5 73 56378.0 74 45197.0 75 33545.0 76 23457.0 77 17696.5 78 14198.5 79 9599.5 80 5233.5 81 2672.5 82 1413.0 83 784.5 84 448.5 85 302.0 86 212.5 87 144.0 88 99.5 89 78.0 90 56.0 91 41.5 92 28.5 93 17.5 94 15.0 95 12.5 96 11.5 97 8.0 98 3.5 99 1.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.30682566627936336 2 0.002302794441054219 3 0.0 4 2.5697851008865924E-4 5 6.674766495809332E-4 6 0.004408683270482064 7 0.008153227274631098 8 0.009855292731062478 9 0.010539456296882934 10-11 0.008859083831562935 12-13 0.012957390459989863 14-15 0.012304932035024502 16-17 0.0172025419513246 18-19 0.009945402078755904 20-21 0.008839059532075507 22-23 0.009866973572430144 24-25 0.01056448667124222 26-27 0.010654596018935645 28-29 0.010617884803208693 30-31 0.010672951626799121 32-33 0.010796434806971594 34-35 0.011650804918435188 36-37 0.008567062797371277 38-39 0.00883405345720365 40-41 0.009641700203196579 42-43 0.008278379146427524 44-45 0.009044308601821644 46-47 0.0073989786606046435 48-49 0.005922186573406829 50-51 0.005338144505023512 52-53 0.0051762814175001365 54-55 0.004493786543303633 56-57 0.006472854809311098 58-59 0.0059372047980224005 60-61 0.004695698229801864 62-63 0.005066147770319282 64-65 0.0026765813648195417 66-67 0.0057936973183625 68-69 0.005441603385708558 70-71 0.006185839849991297 72-73 0.006452830509823672 74-75 0.0038763706424412695 76-77 0.004809169260230624 78-79 0.001298242083434915 80-81 0.013234393269565952 82-83 0.016408244738323287 84-85 0.01791173589150434 86-87 0.012658694659302397 88-89 0.006653073504697952 90-91 0.0020591654639571786 92-93 0.0048709108503168595 94-95 0.0021459374284026997 96-97 0.002397909863619502 98-99 0.0018205425617319952 100 0.009831931048327145 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 2.9963595E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 47.60378795687174 #Duplication Level Percentage of deduplicated Percentage of total 1 73.51944975874017 34.99804297020952 2 13.47016609427823 12.824618609917282 3 5.020223950078005 7.169450292465672 4 2.4099644654342263 4.588937495845066 5 1.3385100453636525 3.1859074188817047 6 0.8234281525681829 2.3518979503544646 7 0.5414866421780946 1.8043770707007147 8 0.3956899154919387 1.50690710670006 9 0.3027485913445245 1.2970781771945723 >10 1.9209858987022317 18.230031905615547 >50 0.19883223019239196 6.418312879394604 >100 0.056948600971574274 4.262595933175049 >500 0.0012053285494248722 0.3725106599677037 >1k 2.2687543741578328E-4 0.1984098908526354 >5k 4.916603645761095E-5 0.16480390138203085 >10k+ 8.42846335397536E-5 0.6261177373433267 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 1.6686916239523328E-5 3.337383247904666E-6 0.0 3.337383247904666E-6 0.0 2 2.6699065983237327E-5 6.674766495809332E-6 0.0 3.337383247904666E-6 0.0 3 2.6699065983237327E-5 1.0012149743713997E-5 0.0 3.337383247904666E-6 0.0 4 4.338598222276065E-5 1.0012149743713997E-5 0.0 3.337383247904666E-6 0.0 5 7.342243145390265E-5 1.3349532991618663E-5 0.0 3.337383247904666E-6 0.0 6 7.675981470180731E-5 2.0024299487427994E-5 0.0 6.674766495809332E-6 0.0 7 9.344673094133064E-5 2.0024299487427994E-5 0.0 6.674766495809332E-6 0.0 8 1.067962639329493E-4 2.0024299487427994E-5 0.0 6.674766495809332E-6 0.0 9 1.368327131640913E-4 2.0024299487427994E-5 0.0 6.674766495809332E-6 0.0 10-11 1.785500037628996E-4 2.0024299487427994E-5 0.0 6.674766495809332E-6 0.0 12-13 3.1705140855094324E-4 2.1692991111380326E-5 0.0 6.674766495809332E-6 0.0 14-15 5.122883285533662E-4 2.5030374359284995E-5 0.0 6.674766495809332E-6 0.0 16-17 8.577074947114991E-4 3.3373832479046656E-5 0.0 6.674766495809332E-6 0.0 18-19 0.0014000322724960074 3.6711215726951325E-5 0.0 6.674766495809332E-6 0.0 20-21 0.002185986027377556 3.6711215726951325E-5 0.0 6.674766495809332E-6 0.0 22-23 0.00324727390021124 4.004859897485599E-5 0.0 8.343458119761664E-6 1.668691623952333E-6 24-25 0.004625613181595867 4.004859897485599E-5 0.0 1.0012149743713997E-5 3.337383247904666E-6 26-27 0.006220882374094297 4.004859897485599E-5 0.0 1.0012149743713997E-5 3.337383247904666E-6 28-29 0.007776102967617871 4.1717290598808316E-5 0.0 2.0024299487427994E-5 3.337383247904666E-6 30-31 0.009064332901309072 4.338598222276065E-5 0.0 2.1692991111380326E-5 5.0060748718569985E-6 32-33 0.00998545067773076 4.5054673846712986E-5 0.0 2.336168273533266E-5 6.674766495809332E-6 34-35 0.010838152097570402 5.0060748718569984E-5 0.0 3.0036449231141993E-5 6.674766495809332E-6 36-37 0.011737576882880709 5.339813196647465E-5 0.0 3.3373832479046656E-5 6.674766495809332E-6 38-39 0.01262532082682335 5.6735515214379316E-5 3.337383247904666E-6 3.3373832479046656E-5 6.674766495809332E-6 40-41 0.013396256357089327 6.007289846228398E-5 3.337383247904666E-6 3.3373832479046656E-5 1.0012149743713997E-5 42-43 0.014228933477441542 6.007289846228398E-5 3.337383247904666E-6 3.3373832479046656E-5 1.3349532991618663E-5 44-45 0.015445409671302793 6.674766495809331E-5 3.337383247904666E-6 3.504252410299899E-5 1.5018224615570996E-5 46-47 0.01723090970893179 7.008504820599798E-5 5.0060748718569985E-6 3.6711215726951325E-5 1.6686916239523328E-5 48-49 0.01943692003579677 7.175373982995032E-5 6.674766495809332E-6 3.6711215726951325E-5 1.6686916239523328E-5 50-51 0.022337106078225928 7.509112307785499E-5 6.674766495809332E-6 3.837990735090366E-5 1.6686916239523328E-5 52-53 0.02606496316613544 7.675981470180731E-5 6.674766495809332E-6 4.338598222276065E-5 1.6686916239523328E-5 54-55 0.03092252448346068 7.842850632575964E-5 6.674766495809332E-6 4.8392057094617656E-5 1.6686916239523328E-5 56-57 0.0374087288257634 8.176588957366431E-5 6.674766495809332E-6 5.172944034252232E-5 1.6686916239523328E-5 58-59 0.04569545143031068 8.343458119761665E-5 8.343458119761664E-6 5.339813196647465E-5 1.6686916239523328E-5 60-61 0.05618985305334691 8.343458119761665E-5 1.0012149743713997E-5 5.339813196647465E-5 1.6686916239523328E-5 62-63 0.06957943464394042 8.343458119761665E-5 1.0012149743713997E-5 5.339813196647465E-5 1.6686916239523328E-5 64-65 0.0866251195826135 8.510327282156897E-5 1.0012149743713997E-5 5.339813196647465E-5 1.6686916239523328E-5 66-67 0.1085500588297232 9.17780393173783E-5 1.0012149743713997E-5 5.339813196647465E-5 1.8355607863475663E-5 68-69 0.13531920986116652 9.67841141892353E-5 1.0012149743713997E-5 5.506682359042698E-5 2.5030374359284995E-5 70-71 0.1687764769214108 1.0012149743713997E-4 1.0012149743713997E-5 5.6735515214379316E-5 2.6699065983237327E-5 72-73 0.21026849415098553 1.0345888068504464E-4 1.0012149743713997E-5 5.6735515214379316E-5 3.003644923114199E-5 74-75 0.2610684732589664 1.1013364718085396E-4 1.0012149743713997E-5 5.840420683833165E-5 3.003644923114199E-5 76-77 0.3237378558881202 1.2014579692456796E-4 1.0012149743713997E-5 6.507897333414098E-5 3.003644923114199E-5 78-79 0.4000654794593239 1.2348318017247264E-4 1.3349532991618663E-5 6.674766495809331E-5 3.003644923114199E-5 80-81 0.49096578698250326 1.2348318017247264E-4 1.3349532991618663E-5 6.674766495809331E-5 3.003644923114199E-5 82-83 0.5988266761715342 1.318266382922343E-4 1.3349532991618663E-5 6.674766495809331E-5 3.3373832479046656E-5 84-85 0.7283738817054496 1.3349532991618662E-4 1.3349532991618663E-5 6.841635658204565E-5 3.3373832479046656E-5 86-87 0.8802114699521202 1.3349532991618662E-4 1.3349532991618663E-5 7.509112307785499E-5 3.3373832479046656E-5 88 1.0091212352856858 1.368327131640913E-4 1.6686916239523328E-5 8.343458119761665E-5 3.3373832479046656E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ATCTCGA 23220 0.0 42.408493 1 TCTCGAG 26010 0.0 37.601704 2 CTCGAGG 34790 0.0 27.881853 3 TATCTCG 10980 0.0 27.565083 2 TCGAGGG 39160 0.0 24.458391 4 GATCCGG 29115 0.0 21.662867 1 TGATACG 3365 0.0 21.228773 3 ATATCTC 14895 0.0 21.045427 1 TACGGCG 3455 0.0 20.131817 6 ATACGGC 3920 0.0 19.182379 5 ATCCGGC 33495 0.0 18.773788 2 CGAGGGC 51720 0.0 18.527945 5 TATGGAT 17580 0.0 17.563955 2 AGGGCGC 55860 0.0 17.088175 7 TCCGGCG 36960 0.0 17.000662 3 GAGGGCG 59780 0.0 16.061338 6 GGGCGCG 60910 0.0 15.864363 8 ATATGGA 20600 0.0 15.400132 1 GGATCCG 20700 0.0 14.5306635 2 GATACGG 4905 0.0 14.372046 4 >>END_MODULE