FastQCFastQC Report
Wed 25 May 2016
SRR491032_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR491032_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40081215
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCTCGA301850.045.6467861
TCTCGAG358450.038.1797942
CTCGAGG460200.029.227283
TATCTCG155250.028.7582722
ATATCTC169900.026.6626071
TCGAGGG517700.025.7087214
ATACGGC39300.023.1994575
GATCCGG462950.023.1643371
TGATACG46250.020.3228033
ATCCGGC527650.020.3076522
TATGGAT280950.018.7018072
CGAGGGC762100.017.6430855
AGGGCGC772350.017.275577
TCCGGCG640000.016.8599873
GGGCGCG825800.016.129018
ACGACGC28300.016.10838740-41
GGATCCG327550.016.0985242
GCGCCGT683450.015.369289
CGGCGCG704950.015.0600555
ATGGATC351250.014.9452173
Produced by FastQC (version 0.11.5)