##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR491032_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	40081215
Sequences flagged as poor quality	0
Sequence length	100
%GC	46
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.70693954761601	31.0	30.0	34.0	26.0	34.0
2	30.04796571161827	31.0	30.0	34.0	26.0	34.0
3	30.164378001016185	31.0	30.0	34.0	26.0	34.0
4	33.63072077530584	35.0	35.0	37.0	28.0	37.0
5	33.14875866412732	35.0	33.0	37.0	26.0	37.0
6	33.02020046049003	35.0	33.0	37.0	26.0	37.0
7	32.94535979011614	35.0	33.0	37.0	26.0	37.0
8	32.88446058833296	35.0	33.0	37.0	26.0	37.0
9	34.19567800028018	38.0	34.0	39.0	26.0	39.0
10-11	34.12934675258721	38.0	34.0	39.0	25.0	39.0
12-13	34.06407959439353	38.0	34.0	39.0	25.0	39.0
14-15	35.025900437399414	39.0	34.0	41.0	25.0	41.0
16-17	34.962733078326096	39.0	34.0	41.0	25.0	41.0
18-19	34.915962552532406	39.0	34.0	41.0	25.0	41.0
20-21	34.85196591470593	39.0	34.0	41.0	24.0	41.0
22-23	34.70051677575144	39.0	34.0	41.0	23.0	41.0
24-25	34.59434555564246	39.0	34.0	41.0	22.5	41.0
26-27	34.39249279244653	38.5	34.0	40.5	21.5	41.0
28-29	34.16517749025323	38.0	33.0	40.0	19.5	41.0
30-31	33.973464639233114	38.0	33.0	40.0	18.5	41.0
32-33	33.726953748782314	38.0	33.0	40.0	16.5	41.0
34-35	33.521947850133785	38.0	33.0	40.0	16.0	41.0
36-37	33.233887919814805	38.0	32.5	40.0	14.0	41.0
38-39	33.05683779795598	38.0	32.0	40.0	11.5	41.0
40-41	32.83957993788361	38.0	32.0	40.0	9.0	41.0
42-43	32.496927875065666	38.0	31.0	40.0	7.5	41.0
44-45	32.133569441445324	37.0	31.0	40.0	2.0	41.0
46-47	31.996276210688723	37.0	31.0	40.0	2.0	41.0
48-49	31.774137148786533	37.0	30.0	40.0	2.0	41.0
50-51	31.528201091209436	37.0	30.0	40.0	2.0	41.0
52-53	31.260392380819795	37.0	30.0	40.0	2.0	41.0
54-55	30.980717500704507	36.0	29.0	40.0	2.0	41.0
56-57	30.6790604900575	36.0	29.0	40.0	2.0	41.0
58-59	30.368804887775983	35.0	28.0	39.0	2.0	41.0
60-61	30.05313711173676	35.0	28.0	39.0	2.0	41.0
62-63	29.794807580059636	35.0	28.0	39.0	2.0	40.5
64-65	29.721688339537614	35.0	28.0	39.0	2.0	40.5
66-67	29.434325082211206	35.0	28.0	38.0	2.0	40.0
68-69	29.063304942227923	35.0	27.5	37.5	2.0	40.0
70-71	28.706114822118042	34.0	27.0	37.0	2.0	39.5
72-73	28.33649093471842	34.0	26.0	36.0	2.0	39.0
74-75	27.975009989093394	34.0	26.0	36.0	2.0	39.0
76-77	27.18507131083726	33.0	25.5	35.0	2.0	37.0
78-79	27.306618087799983	34.0	26.0	35.0	2.0	37.0
80-81	27.135101094116035	34.0	25.5	35.0	2.0	37.0
82-83	26.844139343081288	34.0	25.0	35.0	2.0	36.0
84-85	26.53713815811222	33.0	24.5	35.0	2.0	36.0
86-87	26.291723180048308	33.0	24.0	35.0	2.0	35.5
88-89	26.052926676499204	33.0	23.0	35.0	2.0	35.0
90-91	25.79981644768004	33.0	21.5	35.0	2.0	35.0
92-93	25.528835602912736	33.0	19.5	35.0	2.0	35.0
94-95	25.25900221587594	33.0	18.5	35.0	2.0	35.0
96-97	24.924899369941755	33.0	8.5	35.0	2.0	35.0
98-99	24.578208931540622	33.0	2.0	35.0	2.0	35.0
100	23.841084557940672	32.0	2.0	34.0	2.0	35.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	0.5243346231433925
1101	2	0.5069952869620806
1101	3	0.4923042484269686
1101	4	0.539141626749938
1101	5	0.6557827752582313
1101	6	0.7835150638897801
1101	7	0.7834639943679491
1101	8	0.7507884530490614
1101	9	0.8547379686880774
1101	10-11	0.8819265019673779
1101	12-13	0.8685827359869904
1101	14-15	0.9290018699745417
1101	16-17	0.8249248875868318
1101	18-19	0.7687979419520516
1101	20-21	0.8405103371164486
1101	22-23	0.8064426558879276
1101	24-25	0.9044062333369212
1101	26-27	0.8873493905213223
1101	28-29	0.8258005628271121
1101	30-31	0.7963085226614339
1101	32-33	0.8767625070608105
1101	34-35	0.9128974033016632
1101	36-37	0.9520563038514211
1101	38-39	0.8247730491292486
1101	40-41	0.820186047071978
1101	42-43	0.8616041559042102
1101	44-45	0.7761503418012339
1101	46-47	0.7071779179419408
1101	48-49	0.7151684289107827
1101	50-51	0.6273719404505655
1101	52-53	0.7725630322185602
1101	54-55	0.5586663182633558
1101	56-57	0.5469650995859645
1101	58-59	0.552317836456151
1101	60-61	0.6091125098143877
1101	62-63	0.6673437117682006
1101	64-65	0.6594513256274332
1101	66-67	0.7205406148356985
1101	68-69	0.726046406034051
1101	70-71	0.7481326818012803
1101	72-73	0.6871336011064599
1101	74-75	0.6381118257816105
1101	76-77	0.6634736449046947
1101	78-79	0.7168303696453506
1101	80-81	0.6955271810748549
1101	82-83	0.611743781004602
1101	84-85	0.5702381948028759
1101	86-87	0.5128629589352833
1101	88-89	0.5689585952477714
1101	90-91	0.4830028307385028
1101	92-93	0.4228661759217154
1101	94-95	0.33108988798565164
1101	96-97	0.3396520870077566
1101	98-99	0.3083865674610742
1101	100	0.29247013510540754
1102	1	0.26374987324565424
1102	2	0.1996683368816612
1102	3	0.23688147835538587
1102	4	0.3198418395325717
1102	5	0.4288497523710575
1102	6	0.4535104686243727
1102	7	0.4037927709295417
1102	8	0.32396890006132395
1102	9	0.2813386442857251
1102	10-11	0.39066292972954386
1102	12-13	0.39845508947441033
1102	14-15	0.3370862771158727
1102	16-17	0.20549825675048083
1102	18-19	0.35162844586572106
1102	20-21	0.4161518800888757
1102	22-23	0.3550060263800816
1102	24-25	0.4120849607320167
1102	26-27	0.38518201882360614
1102	28-29	0.41003859712284196
1102	30-31	0.3390674844580275
1102	32-33	0.34712644448179475
1102	34-35	0.3132371860644412
1102	36-37	0.4842507993304963
1102	38-39	0.4187163472904132
1102	40-41	0.3156797151363975
1102	42-43	0.30639708919208175
1102	44-45	0.34872534280653866
1102	46-47	0.2555987714273371
1102	48-49	0.03342914922530227
1102	50-51	0.05026556130032134
1102	52-53	0.19605538552775315
1102	54-55	0.30291811845258465
1102	56-57	0.17454416820858043
1102	58-59	-9.235675953256361E-4
1102	60-61	0.07918313324023529
1102	62-63	0.1295611243184993
1102	64-65	0.20700601659097373
1102	66-67	0.11220525165164119
1102	68-69	0.16887010774265931
1102	70-71	0.09522067762822317
1102	72-73	0.1863290953851724
1102	74-75	0.054576747676797766
1102	76-77	0.08076711454809882
1102	78-79	0.09114911397222869
1102	80-81	0.11907714550553905
1102	82-83	0.10906439116574163
1102	84-85	0.0986124112522262
1102	86-87	-0.03259550846432191
1102	88-89	0.07997469577493277
1102	90-91	0.13091035431293108
1102	92-93	0.036245252930608984
1102	94-95	0.0823625978311675
1102	96-97	-0.005435562511955538
1102	98-99	0.032448253301513574
1102	100	-0.037190422739637796
1103	1	-0.01741723147214813
1103	2	-0.04985176494501786
1103	3	0.0016899634150142617
1103	4	0.015096038509717857
1103	5	0.07876942892458771
1103	6	0.057227746677611435
1103	7	0.04911652225893448
1103	8	0.05022881873927787
1103	9	-0.059554147448785955
1103	10-11	-0.11581079113948078
1103	12-13	-0.008228018968495121
1103	14-15	-0.08065821820479613
1103	16-17	6.151434224932473E-5
1103	18-19	-0.06483151254219877
1103	20-21	0.00452309297951814
1103	22-23	-0.02075043776858365
1103	24-25	0.08735455532201541
1103	26-27	0.02911781512062106
1103	28-29	0.011844241433003333
1103	30-31	-0.025022786409763853
1103	32-33	0.010972387172635933
1103	34-35	0.03225520478463295
1103	36-37	0.13326742873903186
1103	38-39	0.0587412841808046
1103	40-41	-0.014210941939971633
1103	42-43	-0.06422551444384084
1103	44-45	-0.06571284380034825
1103	46-47	-0.0062428808818566495
1103	48-49	-0.11923239532847418
1103	50-51	-0.13446750277739028
1103	52-53	-0.06030471781830826
1103	54-55	-0.07538661997120855
1103	56-57	-0.13232992385911402
1103	58-59	-0.029689226797042778
1103	60-61	-0.013234542478809175
1103	62-63	0.04122908274407777
1103	64-65	0.06395087736915528
1103	66-67	0.09669154982035977
1103	68-69	0.11136285218372777
1103	70-71	0.14236692590243294
1103	72-73	0.042172562125362134
1103	74-75	0.0495184481638411
1103	76-77	0.08670817265822706
1103	78-79	0.14287499290119143
1103	80-81	0.13823624665132073
1103	82-83	0.12238593833798106
1103	84-85	0.10840631263547351
1103	86-87	0.06021027506122678
1103	88-89	0.11147630486031446
1103	90-91	0.04147551066407473
1103	92-93	0.10797324826105026
1103	94-95	0.04594440545871592
1103	96-97	0.022024635840622864
1103	98-99	-0.14297404377635203
1103	100	-0.12721527587008552
1104	1	-0.2994730528315337
1104	2	-0.25805867008526207
1104	3	-0.31881969370172314
1104	4	-0.2033157740572875
1104	5	-0.30989041608859225
1104	6	-0.36606173384326013
1104	7	-0.2876149774253278
1104	8	-0.16215871180876462
1104	9	-0.3126468542668235
1104	10-11	-0.41533221398626097
1104	12-13	-0.40188164263982173
1104	14-15	-0.4225541388512397
1104	16-17	-0.35351020403064837
1104	18-19	-0.43574568165028893
1104	20-21	-0.373794799237011
1104	22-23	-0.31808733718223436
1104	24-25	-0.359656369577003
1104	26-27	-0.4180811321702649
1104	28-29	-0.40104351247752135
1104	30-31	-0.3031612515822246
1104	32-33	-0.3010093309019837
1104	34-35	-0.35586591652617017
1104	36-37	-0.3315886509526109
1104	38-39	-0.4115614247636472
1104	40-41	-0.44050551834802576
1104	42-43	-0.4751223123195203
1104	44-45	-0.4189468263878524
1104	46-47	-0.4307968457934592
1104	48-49	-0.40701836978181305
1104	50-51	-0.36317859192028834
1104	52-53	-0.44430446044844984
1104	54-55	-0.5873569806530234
1104	56-57	-0.35997965612534344
1104	58-59	-0.3033762859317868
1104	60-61	-0.35874171351678896
1104	62-63	-0.473218097869438
1104	64-65	-0.41675817402211024
1104	66-67	-0.35772026828762904
1104	68-69	-0.3958049808884354
1104	70-71	-0.42208964206562527
1104	72-73	-0.3717547597354205
1104	74-75	-0.4274064921115901
1104	76-77	-0.45359749798364035
1104	78-79	-0.4534956296324033
1104	80-81	-0.4415643072380391
1104	82-83	-0.5063978943827045
1104	84-85	-0.49248700744968943
1104	86-87	-0.5034881810620213
1104	88-89	-0.494847504365989
1104	90-91	-0.5012312101709959
1104	92-93	-0.5244411700480605
1104	94-95	-0.5050925759925029
1104	96-97	-0.7430887546575988
1104	98-99	-0.6778253225896371
1104	100	-0.6512927767397052
1105	1	-0.649444898448813
1105	2	-0.6258765746001771
1105	3	-0.6479641401341247
1105	4	-0.5668849765533963
1105	5	-0.6102694213502531
1105	6	-0.8043318212912638
1105	7	-0.7062813717584362
1105	8	-0.7111965822312882
1105	9	-0.8687627546674719
1105	10-11	-0.8355919977043342
1105	12-13	-0.9572149398116778
1105	14-15	-1.0885003263072974
1105	16-17	-1.0041198858939495
1105	18-19	-0.9743104192858638
1105	20-21	-0.9535879442057151
1105	22-23	-0.9005194097176314
1105	24-25	-0.9006022579973134
1105	26-27	-0.9688383437281303
1105	28-29	-1.0473871945661912
1105	30-31	-0.9989314153821738
1105	32-33	-0.9924646658690577
1105	34-35	-0.9328081841786826
1105	36-37	-0.8234818932547938
1105	38-39	-0.9750842153466692
1105	40-41	-1.0431956503398894
1105	42-43	-0.9450969350091292
1105	44-45	-0.9124583196636209
1105	46-47	-0.8629013329005488
1105	48-49	-0.8802314846462842
1105	50-51	-0.8515416262381237
1105	52-53	-0.9505363212196869
1105	54-55	-0.9014910957824505
1105	56-57	-0.9309136974195944
1105	58-59	-0.8923402184732616
1105	60-61	-0.8285594835263765
1105	62-63	-0.8831104129356895
1105	64-65	-0.8870471749911424
1105	66-67	-0.8763301022611572
1105	68-69	-0.829159463526711
1105	70-71	-0.7951861879723161
1105	72-73	-0.7755080635268818
1105	74-75	-0.8270237064205261
1105	76-77	-0.8077830932288492
1105	78-79	-0.7288761987450094
1105	80-81	-0.662851093517741
1105	82-83	-0.6691607657078258
1105	84-85	-0.6387187004023218
1105	86-87	-0.6376381481909128
1105	88-89	-0.7323878240394066
1105	90-91	-0.8594175281825471
1105	92-93	-0.726442913583984
1105	94-95	-0.7150182366821092
1105	96-97	-0.7191721845886576
1105	98-99	-0.7446462266577747
1105	100	-0.7767414769956282
1106	1	-1.1064827433844684
1106	2	-1.1277895264285576
1106	3	-1.117137372128191
1106	4	-1.1269637971905269
1106	5	-1.2338829257924289
1106	6	-1.235614160418823
1106	7	-1.340326226029653
1106	8	-1.3387626956467287
1106	9	-1.5941269122415918
1106	10-11	-1.5498018430920553
1106	12-13	-1.5908259744654174
1106	14-15	-1.829735182154721
1106	16-17	-1.8084346580851118
1106	18-19	-1.9115989563761602
1106	20-21	-1.8242749161817855
1106	22-23	-1.9039794904504106
1106	24-25	-1.8387930905906202
1106	26-27	-1.9076314264071712
1106	28-29	-1.9387846729454878
1106	30-31	-1.8700878087649144
1106	32-33	-1.8820002390703863
1106	34-35	-1.88525281984343
1106	36-37	-1.7257473481332113
1106	38-39	-1.8994091527166006
1106	40-41	-1.894054495013897
1106	42-43	-1.9770946102216698
1106	44-45	-1.9534573332378926
1106	46-47	-1.914987492579499
1106	48-49	-1.8625979919452824
1106	50-51	-1.894102337785938
1106	52-53	-1.9064693144576985
1106	54-55	-1.8904972923704513
1106	56-57	-1.9888350362599319
1106	58-59	-1.956109426515109
1106	60-61	-1.954233540595169
1106	62-63	-1.84552766372288
1106	64-65	-1.808478908046002
1106	66-67	-1.9789253771101514
1106	68-69	-1.89544728916308
1106	70-71	-1.789302188268021
1106	72-73	-1.8321259595004058
1106	74-75	-1.7741275789421742
1106	76-77	-1.780922097845643
1106	78-79	-1.6587147669247102
1106	80-81	-1.6543339213909114
1106	82-83	-1.610349312408406
1106	84-85	-1.7071680744512463
1106	86-87	-1.6952944671588845
1106	88-89	-1.6343408888413506
1106	90-91	-1.6189158731155047
1106	92-93	-1.6456862608865634
1106	94-95	-1.6456727954780668
1106	96-97	-1.6527781725282296
1106	98-99	-1.6730134235779168
1106	100	-1.6865635771403866
1107	1	-1.7018646096959387
1107	2	-1.677098627389153
1107	3	-1.6979106309771055
1107	4	-1.7351783607709237
1107	5	-2.0559290819822955
1107	6	-2.0936490733180193
1107	7	-2.143041813294783
1107	8	-2.0880584264476347
1107	9	-2.3267621098683477
1107	10-11	-2.379964562277948
1107	12-13	-2.397975097539245
1107	14-15	-2.6680832102293692
1107	16-17	-2.7425067505406773
1107	18-19	-2.8022145728550356
1107	20-21	-2.7791619690054006
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1308	4	-1.7014021490591915
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2108	14-15	-2.6562021236855315
2108	16-17	-2.67479653625859
2108	18-19	-2.6720619230818485
2108	20-21	-2.9167605520818753
2108	22-23	-2.800753607413256
2108	24-25	-2.834525542300401
2108	26-27	-2.7314438196879607
2108	28-29	-2.767641656337357
2108	30-31	-2.7177795437890993
2108	32-33	-2.8804165704961378
2108	34-35	-3.2670454521065793
2108	36-37	-2.9574288343392148
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2203	3	1.1666444964547829
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2203	5	1.2344971842325378
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2203	7	1.4452130412557622
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2203	9	1.6264880731563949
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2203	30-31	1.9143668199060926
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2308	88-89	-2.9037736458773225
2308	90-91	-2.680307598351657
2308	92-93	-2.5557488327984608
2308	94-95	-2.4424219968249155
2308	96-97	-2.879373602331679
2308	98-99	-2.739881134412162
2308	100	-2.640204114781497
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	1432733.0
3	392441.0
4	205483.0
5	162511.0
6	159163.0
7	175098.0
8	200020.0
9	217385.0
10	230114.0
11	230576.0
12	239024.0
13	276197.0
14	347895.0
15	436463.0
16	484803.0
17	439822.0
18	358262.0
19	293228.0
20	274962.0
21	282259.0
22	306042.0
23	351266.0
24	413347.0
25	495759.0
26	593127.0
27	700776.0
28	827042.0
29	970484.0
30	1141349.0
31	1347502.0
32	1617151.0
33	1982384.0
34	2503250.0
35	3299949.0
36	4593901.0
37	6097081.0
38	5027586.0
39	973165.0
40	1615.0
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	21.956551937573856	27.747868516231943	27.610632340205377	22.684947205988824
2	24.110088197023853	27.281651637823543	27.014719763322915	21.59354040182969
3	23.899804434571156	26.129372575157717	26.446803072212255	23.524019918058872
4	24.81831912377922	25.773626497715345	26.239172242918997	23.168882135586443
5	25.109645456985056	25.749073052162068	25.87454146607705	23.266740024775824
6	25.119139046656585	25.590253596015295	25.38861361478682	23.901993742541293
7	24.99189097073314	26.042856094921053	25.52321951915702	23.442033415188785
8	24.609014202445163	25.676985880891685	25.837002608143905	23.876997308519247
9	24.408518824014525	25.47909315293629	25.945744353139972	24.16664366990922
10-11	24.314892657205426	25.659692289727616	25.971237525453105	24.054177527613856
12-13	24.011996422377038	25.750091101732686	26.091468871827004	24.14644360406327
14-15	23.73053382396159	25.97470546759868	26.529253477641397	23.765507230798335
16-17	23.52754906747488	25.89036366212813	26.801372322534924	23.78071494786207
18-19	23.128954391547317	25.889376735251446	27.491010067778436	23.490658805422797
20-21	22.756555719884165	25.630281040844032	28.326042175731846	23.287121063539956
22-23	22.76534825362779	25.585263445883648	28.73431063283065	22.91507766765791
24-25	22.66161975208196	25.45479594283998	29.165281454073504	22.718302851004555
26-27	22.67846531858793	25.48778039836045	29.26028041551449	22.573473867537135
28-29	22.70590910950913	25.623487329245982	29.190835298661717	22.479768262583175
30-31	22.678605511593663	25.506094896283894	29.18717803395854	22.628121558163905
32-33	22.726757879885565	25.59014782958936	29.073855784855922	22.609238505669154
34-35	22.829866961266436	25.632571555624068	29.059312448111957	22.47824903499754
36-37	22.950072903460224	25.573780329009026	28.908134778615192	22.568011988915554
38-39	22.828082164676573	25.724644208183346	28.836524218864344	22.61074940827574
40-41	22.905800517760124	25.842118717049743	28.62674051025733	22.625340254932798
42-43	23.045764422860163	25.91304549819701	28.195682635135878	22.845507443806948
44-45	23.102235666260224	26.157242598020037	27.779593516903933	22.960928218815805
46-47	23.33569442030462	26.332137844364272	27.31326890956705	23.018898825764058
48-49	23.424709248330338	26.380536881077997	26.913331324572454	23.281422546019208
50-51	23.43886609302281	26.49034524832743	26.699700334436443	23.371088324213314
52-53	23.519529372583854	26.629845859501156	26.48703880624766	23.363585961667336
54-55	23.578484134908788	26.591555301396063	26.416065491312086	23.41389507238306
56-57	23.53211164085553	26.65350530308373	26.413060017532047	23.401323038528695
58-59	23.598253288183685	26.756629964227802	26.33214283954382	23.312973908044693
60-61	23.537901983905414	26.735624381061097	26.313860815715532	23.412612819317957
62-63	23.478470040313784	26.876440009668762	26.327265257590447	23.31782469242701
64-65	23.51220594290783	27.010116587914784	26.266127868465695	23.21154960071169
66-67	23.50479708067311	27.04177704762669	26.170685641413176	23.28274023028702
68-69	23.51037465806524	27.167481811852007	26.075508666651253	23.2466348634315
70-71	23.570390957182806	27.306148342076035	26.00597883160721	23.117481869133954
72-73	23.50284135012123	27.35731334463729	25.867981700027542	23.271863605213937
74-75	23.475018338974103	27.53453903537433	25.83725734526406	23.153185280387515
76-77	23.38212116017921	27.721493215887254	25.689662614487595	23.206723009445938
78-79	23.39061261230445	27.79526103626196	25.605106015486324	23.20902033594727
80-81	23.34258167613428	27.907741196167663	25.575881731303447	23.17379539639461
82-83	23.340389413688555	28.04250202316142	25.4836267219954	23.133481841154623
84-85	23.2939615271469	28.12074919455928	25.37608589983591	23.20920337845791
86-87	23.27723016923648	28.297098346024736	25.270950978231433	23.15472050650735
88-89	23.371717449001487	28.407842704378616	25.146094021401165	23.07434582521873
90-91	23.33745396968427	28.448318814550134	25.073584009771967	23.140643205993637
92-93	23.359103918722575	28.58402451754209	24.95776271502679	23.099108848708553
94-95	23.41806353693675	28.690697311311546	24.8505492729944	23.040689878757302
96-97	23.375733085204576	28.804786375419766	24.659742777156218	23.159737762219443
98-99	23.35316105232256	28.938809167464218	24.52676027252201	23.181269507691212
100	23.466151860756877	29.100247422227675	24.15415582510173	23.279444891913712
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	4213.0
1	2817.5
2	1353.5
3	1292.5
4	1402.0
5	1674.0
6	1951.5
7	2249.5
8	2656.5
9	3200.5
10	3914.5
11	4829.5
12	6254.5
13	8850.5
14	15339.5
15	21512.0
16	29849.0
17	33231.0
18	31864.5
19	37662.0
20	46395.0
21	56623.5
22	70464.0
23	86745.0
24	104459.5
25	124956.0
26	150301.5
27	183843.5
28	223213.5
29	261282.5
30	299794.0
31	350240.5
32	415835.0
33	488323.0
34	561180.0
35	651078.0
36	762050.0
37	898009.0
38	1043681.5
39	1148076.0
40	1221072.0
41	1318556.0
42	1471764.0
43	1623106.5
44	1735084.0
45	1827296.0
46	1854773.0
47	1831130.5
48	1813837.0
49	1823021.5
50	1756713.5
51	1610510.5
52	1443939.5
53	1254410.5
54	1108248.5
55	1008437.0
56	913840.0
57	814180.5
58	740314.0
59	680578.5
60	609345.5
61	544115.5
62	486720.5
63	428288.5
64	373370.0
65	320229.5
66	271231.0
67	227248.5
68	185529.0
69	148015.5
70	117228.5
71	92208.5
72	73153.5
73	58202.0
74	45561.5
75	34047.0
76	24051.5
77	17344.5
78	13361.0
79	9080.0
80	4980.5
81	2577.0
82	1444.5
83	861.0
84	512.0
85	331.5
86	218.5
87	149.0
88	112.5
89	84.0
90	61.0
91	54.5
92	39.0
93	25.5
94	21.0
95	11.5
96	10.5
97	8.5
98	3.0
99	1.5
100	1.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.3016525322398535
2	0.0023726825646378236
3	0.0
4	2.095744852046027E-4
5	5.264311473591806E-4
6	0.0034080803189224674
7	0.006075165136585805
8	0.007247784279992509
9	0.007736791412136583
10-11	0.006499303975690357
12-13	0.009397170220513526
14-15	0.00903415727292698
16-17	0.01228006685925065
18-19	0.007259011484557043
20-21	0.006600348816771148
22-23	0.007278970959338433
24-25	0.007811639442566799
26-27	0.007958840569079556
28-29	0.007721821806050541
30-31	0.007817876778435984
32-33	0.007840331187565048
34-35	0.008328090852535283
36-37	0.006305946563745635
38-39	0.006439425551346185
40-41	0.007010765516963495
42-43	0.006142528363972998
44-45	0.006606586152640333
46-47	0.0055312694487929075
48-49	0.004442230606033276
50-51	0.004245130792567042
52-53	0.004021834168450233
54-55	0.0034804334150050093
56-57	0.005075943930342431
58-59	0.004759087268187853
60-61	0.003822239420636325
62-63	0.004049278446274645
64-65	0.0022529257159494788
66-67	0.004588184265372195
68-69	0.004364887641255386
70-71	0.00493872254122037
72-73	0.005062221791430225
74-75	0.0031785463589364747
76-77	0.0038546735671560854
78-79	0.0012237652975340195
80-81	0.01343896386374515
82-83	0.016361779452045054
84-85	0.01794356782847027
86-87	0.012954946600296424
88-89	0.006846099850017022
90-91	0.0020857651146553315
92-93	0.004973651622087803
94-95	0.0019485437255332705
96-97	0.0023676926959424758
98-99	0.0017701559196745906
100	0.00991736403200352
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	4.0081215E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	34.18213162932625
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.61960365678453	25.506571143243633
2	12.437934152087475	8.503102047670929
3	4.605385908131742	4.722657219468104
4	2.239141929858389	3.0615457673265225
5	1.3550282836661467	2.3158877576868124
6	0.8712081722006005	1.7867851451223395
7	0.5817506357319497	1.3919833764223615
8	0.4164793922204546	1.1388922724625101
9	0.30125617886422806	0.9267820524076406
>10	2.021261503158376	14.25244248167245
>50	0.31032061757495666	7.28434101580941
>100	0.18942561918383596	13.276873912319779
>500	0.03753445660992247	8.679958052183913
>1k	0.013522428472494689	6.259946191647736
>5k	5.147290954874532E-5	0.1347791853491978
>10k+	9.559254515683487E-5	0.757452379206659
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.2474671738369208E-5	2.4949343476738416E-6	0.0	0.0	0.0
2	2.744427782441226E-5	2.4949343476738416E-6	0.0	2.4949343476738416E-6	2.4949343476738416E-6
3	4.7403752605802996E-5	7.484803043021525E-6	0.0	2.4949343476738416E-6	2.4949343476738416E-6
4	6.736322738719372E-5	7.484803043021525E-6	0.0	2.4949343476738416E-6	2.4949343476738416E-6
5	8.732270216858445E-5	1.2474671738369208E-5	0.0	2.4949343476738416E-6	2.4949343476738416E-6
6	1.0728217694997519E-4	1.2474671738369208E-5	0.0	2.4949343476738416E-6	2.4949343476738416E-6
7	1.197568486883444E-4	1.2474671738369208E-5	0.0	2.4949343476738416E-6	2.4949343476738416E-6
8	1.4969606086043052E-4	1.2474671738369208E-5	0.0	4.989868695347683E-6	2.4949343476738416E-6
9	1.621707325987997E-4	1.2474671738369208E-5	0.0	4.989868695347683E-6	2.4949343476738416E-6
10-11	1.9460487911855966E-4	1.496960608604305E-5	0.0	4.989868695347683E-6	4.989868695347683E-6
12-13	2.68205442374938E-4	1.8712007607553815E-5	0.0	4.989868695347683E-6	4.989868695347683E-6
14-15	4.0916923301851006E-4	2.1206941955227655E-5	0.0	4.989868695347683E-6	4.989868695347683E-6
16-17	6.861069456103065E-4	2.3701876302901494E-5	0.0	4.989868695347683E-6	4.989868695347683E-6
18-19	0.001057852163413709	2.6196810650575338E-5	0.0	4.989868695347683E-6	6.237335869184604E-6
20-21	0.001594263048163585	3.243414651975994E-5	0.0	4.989868695347683E-6	7.484803043021525E-6
22-23	0.0023876521707238666	3.368161369359687E-5	0.0	4.989868695347683E-6	9.979737390695366E-6
24-25	0.0033781411067503814	3.4929080867433786E-5	0.0	4.989868695347683E-6	1.2474671738369208E-5
26-27	0.004674259500366942	3.6176548041270704E-5	0.0	4.989868695347683E-6	1.621707325987997E-5
28-29	0.0058381463735567895	3.742401521510763E-5	0.0	4.989868695347683E-6	2.1206941955227655E-5
30-31	0.006717610731111819	4.366135108429223E-5	0.0	4.989868695347683E-6	2.4949343476738416E-5
32-33	0.007244041878471	5.2393621301150675E-5	0.0	4.989868695347683E-6	2.6196810650575338E-5
34-35	0.007811639442566798	5.738348999649836E-5	0.0	6.237335869184604E-6	2.744427782441226E-5
36-37	0.008522695731653844	5.863095717033528E-5	0.0	7.484803043021525E-6	3.4929080867433786E-5
38-39	0.009147676785746142	5.98784243441722E-5	0.0	1.2474671738369208E-5	3.4929080867433786E-5
40-41	0.009869960279397717	5.98784243441722E-5	0.0	1.2474671738369208E-5	3.742401521510763E-5
42-43	0.0108492220108597	6.486829303951989E-5	0.0	1.496960608604305E-5	3.742401521510763E-5
44-45	0.012250127647078564	6.861069456103065E-5	0.0	1.496960608604305E-5	3.867148238894455E-5
46-47	0.014328407958690873	6.985816173486757E-5	0.0	1.496960608604305E-5	4.366135108429223E-5
48-49	0.017283657693510538	7.360056325637833E-5	0.0	1.7464540433716893E-5	4.490881825812915E-5
50-51	0.02124935583913811	7.484803043021526E-5	0.0	2.2454409129064576E-5	4.615628543196608E-5
52-53	0.026798089828364735	7.983789912556293E-5	1.2474671738369208E-6	2.4949343476738416E-5	4.989868695347683E-5
54-55	0.03424921125769266	8.482776782091062E-5	2.4949343476738416E-6	2.4949343476738416E-5	5.488855564882452E-5
56-57	0.044212731575128145	8.482776782091062E-5	2.4949343476738416E-6	2.4949343476738416E-5	5.488855564882452E-5
58-59	0.05701423971304263	8.857016934242138E-5	2.4949343476738416E-6	2.4949343476738416E-5	5.613602282266144E-5
60-61	0.07323879777596562	9.356003803776907E-5	2.4949343476738416E-6	2.4949343476738416E-5	5.738348999649836E-5
62-63	0.0937371783764539	9.480750521160599E-5	3.7424015215107624E-6	2.4949343476738416E-5	5.738348999649836E-5
64-65	0.11940755787967006	9.730243955927983E-5	4.989868695347683E-6	2.4949343476738416E-5	5.738348999649836E-5
66-67	0.15164959445465914	1.0229230825462751E-4	4.989868695347683E-6	2.744427782441226E-5	5.738348999649836E-5
68-69	0.19165212431808765	1.0229230825462751E-4	4.989868695347683E-6	2.744427782441226E-5	5.738348999649836E-5
70-71	0.24078486642682861	1.0229230825462751E-4	4.989868695347683E-6	2.744427782441226E-5	5.863095717033528E-5
72-73	0.3004911403010113	1.0478724260230135E-4	4.989868695347683E-6	2.744427782441226E-5	6.486829303951989E-5
74-75	0.37217060410968084	1.0852964412381211E-4	4.989868695347683E-6	2.744427782441226E-5	6.861069456103065E-5
76-77	0.45852651921854165	1.135195128191598E-4	4.989868695347683E-6	2.744427782441226E-5	7.235309608254141E-5
78-79	0.5603872038310216	1.1476697999299672E-4	4.989868695347683E-6	2.744427782441226E-5	7.235309608254141E-5
80-81	0.6802039309437102	1.1726191434067056E-4	4.989868695347683E-6	2.744427782441226E-5	7.360056325637833E-5
82-83	0.8192254151976182	1.2225178303601825E-4	4.989868695347683E-6	2.744427782441226E-5	7.8590431951726E-5
84-85	0.9796758905636966	1.2724165173136592E-4	4.989868695347683E-6	2.99392121720861E-5	7.983789912556293E-5
86-87	1.164171295705482	1.2973658607903977E-4	4.989868695347683E-6	3.243414651975994E-5	8.35803006470737E-5
88	1.3188746898016939	1.2973658607903977E-4	4.989868695347683E-6	3.4929080867433786E-5	8.732270216858445E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATCTCGA	30185	0.0	45.646786	1
TCTCGAG	35845	0.0	38.179794	2
CTCGAGG	46020	0.0	29.22728	3
TATCTCG	15525	0.0	28.758272	2
ATATCTC	16990	0.0	26.662607	1
TCGAGGG	51770	0.0	25.708721	4
ATACGGC	3930	0.0	23.199457	5
GATCCGG	46295	0.0	23.164337	1
TGATACG	4625	0.0	20.322803	3
ATCCGGC	52765	0.0	20.307652	2
TATGGAT	28095	0.0	18.701807	2
CGAGGGC	76210	0.0	17.643085	5
AGGGCGC	77235	0.0	17.27557	7
TCCGGCG	64000	0.0	16.859987	3
GGGCGCG	82580	0.0	16.12901	8
ACGACGC	2830	0.0	16.108387	40-41
GGATCCG	32755	0.0	16.098524	2
GCGCCGT	68345	0.0	15.36928	9
CGGCGCG	70495	0.0	15.060055	5
ATGGATC	35125	0.0	14.945217	3
>>END_MODULE