FastQCFastQC Report
Wed 25 May 2016
SRR491031_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR491031_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49011417
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCTCGA322700.049.63751
ATACGGC63500.042.2611125
TGATACG66600.040.293473
TCTCGAG421150.037.8305172
CTCGAGG487250.032.206043
ACGACGC49900.026.84264240-41
TCGAGGG587550.026.5482044
GATCCGG499300.024.9623451
ATGATAC115050.024.346642
TATCTCG190200.024.2650052
ATATCTC197200.023.856631
TACGGCG108200.023.672776
CGATCTA44500.021.80817252-53
CGACGCT62200.021.269942-43
ATCCGGC585800.021.1402762
GATACGG130650.020.8277284
ACGGCGA134800.018.8630567
ACGCTCT71800.018.85130944-45
AGGGCGC881300.017.6099457
CGGCGAC150550.016.827238
Produced by FastQC (version 0.11.5)