##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR491031_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	49011417
Sequences flagged as poor quality	0
Sequence length	100
%GC	46
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.653771895638112	31.0	30.0	34.0	26.0	34.0
2	30.002062662256836	31.0	30.0	34.0	26.0	34.0
3	30.12266709203694	31.0	30.0	34.0	26.0	34.0
4	33.587000514594386	35.0	35.0	37.0	28.0	37.0
5	33.106369603637454	35.0	33.0	37.0	26.0	37.0
6	32.977419322522344	35.0	33.0	37.0	26.0	37.0
7	32.90019243883522	35.0	33.0	37.0	26.0	37.0
8	32.83642884677258	35.0	33.0	37.0	26.0	37.0
9	34.144522509928656	38.0	34.0	39.0	25.0	39.0
10-11	34.07694265399427	38.0	34.0	39.0	25.0	39.0
12-13	34.00861437040271	38.0	34.0	39.0	25.0	39.0
14-15	34.95859326001531	39.0	34.0	41.0	25.0	41.0
16-17	34.89557020969217	39.0	34.0	41.0	25.0	41.0
18-19	34.84348444771552	39.0	34.0	41.0	24.5	41.0
20-21	34.77793068092686	39.0	34.0	41.0	24.0	41.0
22-23	34.616606759196536	39.0	34.0	41.0	23.0	41.0
24-25	34.50473993641114	39.0	34.0	41.0	22.5	41.0
26-27	34.2993570253233	38.0	33.5	40.0	20.5	41.0
28-29	34.07313216428736	38.0	33.0	40.0	19.0	41.0
30-31	33.881507455701595	38.0	33.0	40.0	18.0	41.0
32-33	33.6358281581616	38.0	33.0	40.0	16.5	41.0
34-35	33.430142531892116	38.0	33.0	40.0	15.5	41.0
36-37	33.136825772656195	38.0	32.0	40.0	12.0	41.0
38-39	32.956108094160996	38.0	32.0	40.0	10.5	41.0
40-41	32.738386129501215	38.0	31.5	40.0	8.5	41.0
42-43	32.39628167657344	37.5	31.0	40.0	7.0	41.0
44-45	32.03354191942665	37.0	30.5	40.0	2.0	41.0
46-47	31.90512768688161	37.0	30.5	40.0	2.0	41.0
48-49	31.68435145427442	37.0	30.0	40.0	2.0	41.0
50-51	31.433153381384585	37.0	30.0	40.0	2.0	41.0
52-53	31.162599512272823	36.5	29.5	40.0	2.0	41.0
54-55	30.873373483570163	36.0	29.0	40.0	2.0	41.0
56-57	30.56201026589376	36.0	28.5	39.5	2.0	41.0
58-59	30.241363252158166	35.0	28.0	39.0	2.0	41.0
60-61	29.911324375706176	35.0	28.0	39.0	2.0	40.5
62-63	29.64243896478243	35.0	28.0	39.0	2.0	40.0
64-65	29.56370960464171	35.0	28.0	38.5	2.0	40.0
66-67	29.257964710548976	35.0	28.0	37.5	2.0	40.0
68-69	28.875027179891575	34.5	27.5	37.0	2.0	39.5
70-71	28.50528684979665	34.0	26.5	36.5	2.0	39.0
72-73	28.132795885089386	34.0	26.0	36.0	2.0	39.0
74-75	27.76831620681361	34.0	26.0	35.5	2.0	37.5
76-77	26.99568123076303	33.0	25.5	35.0	2.0	37.0
78-79	27.12542390888229	33.5	26.0	35.0	2.0	37.0
80-81	26.969199309214012	34.0	25.0	35.0	2.0	36.0
82-83	26.702430772405542	33.5	25.0	35.0	2.0	36.0
84-85	26.417568461650475	33.0	24.0	35.0	2.0	35.5
86-87	26.195762234338176	33.0	24.0	35.0	2.0	35.0
88-89	25.978552793117572	33.0	23.0	35.0	2.0	35.0
90-91	25.740400150030347	33.0	21.5	35.0	2.0	35.0
92-93	25.48385949543144	33.0	19.5	35.0	2.0	35.0
94-95	25.22689081811285	33.0	18.5	35.0	2.0	35.0
96-97	24.902280778782625	33.0	10.0	35.0	2.0	35.0
98-99	24.561342278677643	33.0	2.0	35.0	2.0	35.0
100	23.798372081345864	32.0	2.0	34.0	2.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.5186296930370631
1101	2	0.49089563393433977
1101	3	0.4895524908201203
1101	4	0.5519301019620073
1101	5	0.6682374303068599
1101	6	0.7260423052979803
1101	7	0.7633004731902489
1101	8	0.7275758043997627
1101	9	0.8300550390364307
1101	10-11	0.8725913956601232
1101	12-13	0.827214410791342
1101	14-15	0.8926150843798979
1101	16-17	0.8048557038965498
1101	18-19	0.7999638637160871
1101	20-21	0.8416884691811717
1101	22-23	0.8193700980090313
1101	24-25	0.8999416273941065
1101	26-27	0.8904757942484167
1101	28-29	0.8069734355209945
1101	30-31	0.804419903634404
1101	32-33	0.8724187638112895
1101	34-35	0.8987634091040846
1101	36-37	0.8988350421892264
1101	38-39	0.7814852898736717
1101	40-41	0.7915522821921996
1101	42-43	0.8011650695503505
1101	44-45	0.6782506321019
1101	46-47	0.6397492200945436
1101	48-49	0.635974540511345
1101	50-51	0.561800841712369
1101	52-53	0.7083406325687029
1101	54-55	0.4936808066727103
1101	56-57	0.5127782894255475
1101	58-59	0.5159008627048252
1101	60-61	0.5761534062626907
1101	62-63	0.599266550461568
1101	64-65	0.584400376025414
1101	66-67	0.640610857644937
1101	68-69	0.6512983993932231
1101	70-71	0.7037095772179391
1101	72-73	0.6433276577321827
1101	74-75	0.6123827945055282
1101	76-77	0.6390269609551922
1101	78-79	0.6652975476718019
1101	80-81	0.6643444963262937
1101	82-83	0.560585532868128
1101	84-85	0.4894646295628071
1101	86-87	0.4450216852771298
1101	88-89	0.5040056178325543
1101	90-91	0.4333435744528593
1101	92-93	0.37319452205620607
1101	94-95	0.2953663710053398
1101	96-97	0.2898894218195167
1101	98-99	0.26851423291798326
1101	100	0.2364082367031699
1102	1	0.2573573110046823
1102	2	0.2186931277230748
1102	3	0.25627953122639724
1102	4	0.3495821433109825
1102	5	0.4416065606198174
1102	6	0.48235601143035467
1102	7	0.4323989453879804
1102	8	0.3358046101556411
1102	9	0.31324491002146004
1102	10-11	0.39159727151974977
1102	12-13	0.4388141087654702
1102	14-15	0.39011828828323303
1102	16-17	0.2709442567644018
1102	18-19	0.3869937765199296
1102	20-21	0.4756782375972364
1102	22-23	0.4141152704802309
1102	24-25	0.4412823846865592
1102	26-27	0.4303808347391964
1102	28-29	0.4590807601256657
1102	30-31	0.42003080480577637
1102	32-33	0.40879484102718067
1102	34-35	0.37026579686227734
1102	36-37	0.5363862687122563
1102	38-39	0.4903839267027337
1102	40-41	0.35549811525385167
1102	42-43	0.3580043077523243
1102	44-45	0.4128703911232421
1102	46-47	0.34956847880628317
1102	48-49	0.13436720739301933
1102	50-51	0.1237393843355612
1102	52-53	0.28472737768637657
1102	54-55	0.4095447901851301
1102	56-57	0.2751597600723379
1102	58-59	0.07969255062632286
1102	60-61	0.16907680770111355
1102	62-63	0.22624880270328518
1102	64-65	0.2786299118557203
1102	66-67	0.1757650019000465
1102	68-69	0.27563768355613405
1102	70-71	0.17541160147987256
1102	72-73	0.2699825886235381
1102	74-75	0.12700585024941802
1102	76-77	0.1605560026107291
1102	78-79	0.17407438926651508
1102	80-81	0.22579144138676455
1102	82-83	0.20414924639186083
1102	84-85	0.1853806385858121
1102	86-87	0.06934134858517993
1102	88-89	0.14889588044010438
1102	90-91	0.2081444173490219
1102	92-93	0.11392825445743071
1102	94-95	0.16337014912405223
1102	96-97	0.052525908264058785
1102	98-99	0.09610818174156677
1102	100	0.027572478457749128
1103	1	-0.003592628256612329
1103	2	-0.0564540193061589
1103	3	-0.0029406722225502335
1103	4	-0.014474360213995396
1103	5	0.049882073785859404
1103	6	0.05803719921334505
1103	7	0.030713927900215765
1103	8	0.005864078020941577
1103	9	-0.07895758151408216
1103	10-11	-0.13066007482698438
1103	12-13	-0.02137916449887456
1103	14-15	-0.10165619901518852
1103	16-17	-0.02602338723156805
1103	18-19	-0.08879676783470813
1103	20-21	-0.00987600827934898
1103	22-23	-0.049865511356316006
1103	24-25	0.045781381439361724
1103	26-27	0.010741553761398848
1103	28-29	0.0025137272640236574
1103	30-31	-0.035283061528701865
1103	32-33	-0.029801484094100772
1103	34-35	0.01918358769836459
1103	36-37	0.1083539677414862
1103	38-39	0.03034717615692273
1103	40-41	-0.0464334741063368
1103	42-43	-0.08364814717479163
1103	44-45	-0.06774189010646836
1103	46-47	-0.018866203149407568
1103	48-49	-0.13144929410000472
1103	50-51	-0.14523379517905255
1103	52-53	-0.10207823089913504
1103	54-55	-0.09055653809776842
1103	56-57	-0.18627797805567425
1103	58-59	-0.052530315112836234
1103	60-61	-0.05471371360262722
1103	62-63	-0.003601601506542096
1103	64-65	0.01927962729819299
1103	66-67	0.0633733157751486
1103	68-69	0.06754685578030717
1103	70-71	0.05709584044715044
1103	72-73	-0.026158303758545998
1103	74-75	-0.004939486517407943
1103	76-77	0.0018144496677159339
1103	78-79	0.04625446505644959
1103	80-81	0.05044168740715094
1103	82-83	0.05263427969333989
1103	84-85	0.020430567646872788
1103	86-87	-0.006932344781514388
1103	88-89	0.010293162731077388
1103	90-91	-0.026803155432087777
1103	92-93	0.01295983755378316
1103	94-95	-0.04491803416903295
1103	96-97	-0.09141877943798349
1103	98-99	-0.22822437953969654
1103	100	-0.18422523362784204
1104	1	-0.3172181141788535
1104	2	-0.2522093701223298
1104	3	-0.31279686165639475
1104	4	-0.20804953149920635
1104	5	-0.30883821873784
1104	6	-0.35025963088038026
1104	7	-0.33200386882136
1104	8	-0.18252187492429783
1104	9	-0.3164334674450018
1104	10-11	-0.4268616898211519
1104	12-13	-0.3927591862450299
1104	14-15	-0.39412688800568674
1104	16-17	-0.329203371944395
1104	18-19	-0.42136251651403
1104	20-21	-0.3393488347651896
1104	22-23	-0.29511471011711876
1104	24-25	-0.3520544641079226
1104	26-27	-0.3877771810418622
1104	28-29	-0.388015856963122
1104	30-31	-0.2727663764185593
1104	32-33	-0.2844581560732493
1104	34-35	-0.2973781761647203
1104	36-37	-0.3144373816986956
1104	38-39	-0.392663428582253
1104	40-41	-0.4491664883824029
1104	42-43	-0.4612068043178539
1104	44-45	-0.41725514286582666
1104	46-47	-0.44189515734306184
1104	48-49	-0.4228260753307822
1104	50-51	-0.3534902558363804
1104	52-53	-0.43747314774666535
1104	54-55	-0.5824644213139258
1104	56-57	-0.3781645225465162
1104	58-59	-0.2915582417641751
1104	60-61	-0.3533073082760758
1104	62-63	-0.47369205936054115
1104	64-65	-0.4064282096651368
1104	66-67	-0.36929991754220026
1104	68-69	-0.39311531335964744
1104	70-71	-0.42217554765658605
1104	72-73	-0.3629970981449553
1104	74-75	-0.43669281764389467
1104	76-77	-0.44816621324043027
1104	78-79	-0.459635912583952
1104	80-81	-0.4671480123096572
1104	82-83	-0.5293390815381365
1104	84-85	-0.5285949344359473
1104	86-87	-0.5080599793657505
1104	88-89	-0.49667911813799037
1104	90-91	-0.49216012473621973
1104	92-93	-0.513930306375304
1104	94-95	-0.5096626516047174
1104	96-97	-0.7642404290193276
1104	98-99	-0.7140224737616769
1104	100	-0.6964807661996772
1105	1	-0.6985538343850841
1105	2	-0.6646781917139535
1105	3	-0.6714010081800055
1105	4	-0.5572593501389846
1105	5	-0.6525208501486759
1105	6	-0.8445989261387155
1105	7	-0.7357960907142171
1105	8	-0.743834531207888
1105	9	-0.8978556132485522
1105	10-11	-0.8620211778311102
1105	12-13	-0.9683726348514909
1105	14-15	-1.0904953503415271
1105	16-17	-1.0375962210928051
1105	18-19	-0.9856823564571471
1105	20-21	-0.9667527174667256
1105	22-23	-0.9375392523208959
1105	24-25	-0.9236461046443409
1105	26-27	-1.015140132818523
1105	28-29	-1.0320518213057994
1105	30-31	-0.9831109057917544
1105	32-33	-0.9889628127413133
1105	34-35	-0.956240785784729
1105	36-37	-0.8391289381859579
1105	38-39	-0.9736550308959551
1105	40-41	-1.0261378987974972
1105	42-43	-0.9505882302002036
1105	44-45	-0.8907355705542912
1105	46-47	-0.8837893904623826
1105	48-49	-0.8928119633416287
1105	50-51	-0.8745684098202311
1105	52-53	-0.9473574060742003
1105	54-55	-0.9090389076579335
1105	56-57	-0.9240303652604176
1105	58-59	-0.8583297366808758
1105	60-61	-0.8137639373605126
1105	62-63	-0.8427546145507918
1105	64-65	-0.863754414290554
1105	66-67	-0.828696204218172
1105	68-69	-0.7784130936671403
1105	70-71	-0.766624679375564
1105	72-73	-0.7419578126451647
1105	74-75	-0.7934535502556059
1105	76-77	-0.7709454131099882
1105	78-79	-0.6831384465209425
1105	80-81	-0.6308821244018787
1105	82-83	-0.6618402418538132
1105	84-85	-0.6241791081214352
1105	86-87	-0.6416850943684658
1105	88-89	-0.7417551845458306
1105	90-91	-0.8685400500665494
1105	92-93	-0.7436477861454627
1105	94-95	-0.7207750055162521
1105	96-97	-0.6735491359187868
1105	98-99	-0.6909286017653464
1105	100	-0.722905759090505
1106	1	-1.070154475601747
1106	2	-1.0922109513826896
1106	3	-1.0897802952135969
1106	4	-1.100184093977056
1106	5	-1.2089757955147178
1106	6	-1.1852205308429546
1106	7	-1.3012316805518047
1106	8	-1.2664645675299404
1106	9	-1.5334143686459072
1106	10-11	-1.4946721976685922
1106	12-13	-1.5462311791142085
1106	14-15	-1.768231007668895
1106	16-17	-1.7481729142411027
1106	18-19	-1.8443589487509229
1106	20-21	-1.7566034556584142
1106	22-23	-1.8265351327658053
1106	24-25	-1.7777411392420674
1106	26-27	-1.8623795337121365
1106	28-29	-1.8738544340175167
1106	30-31	-1.8024076794224868
1106	32-33	-1.7913726088575714
1106	34-35	-1.8248104096332654
1106	36-37	-1.6686680689388282
1106	38-39	-1.8388038952469081
1106	40-41	-1.8534159804189763
1106	42-43	-1.9056091320430752
1106	44-45	-1.879138052985283
1106	46-47	-1.8803516545733032
1106	48-49	-1.8040463820716752
1106	50-51	-1.8334146398617257
1106	52-53	-1.809162225586281
1106	54-55	-1.812419185938193
1106	56-57	-1.918032575450269
1106	58-59	-1.8959250621862083
1106	60-61	-1.8916656176786866
1106	62-63	-1.8040306198366913
1106	64-65	-1.7391389334018754
1106	66-67	-1.9037099473667745
1106	68-69	-1.7845003991340214
1106	70-71	-1.6988898687482425
1106	72-73	-1.7567294773746234
1106	74-75	-1.688574366921884
1106	76-77	-1.7000743625532202
1106	78-79	-1.6136781515905838
1106	80-81	-1.5966655803269276
1106	82-83	-1.5903449077610752
1106	84-85	-1.691967866332572
1106	86-87	-1.6823672116749897
1106	88-89	-1.5962113368949211
1106	90-91	-1.6071208143164775
1106	92-93	-1.6389825372225424
1106	94-95	-1.6204415873240272
1106	96-97	-1.6117492837724718
1106	98-99	-1.6524350830786183
1106	100	-1.6728546612710211
1107	1	-1.6114470772262983
1107	2	-1.595587479424946
1107	3	-1.627047764034355
1107	4	-1.6783586236235237
1107	5	-1.9445486257933524
1107	6	-1.9773498215544443
1107	7	-2.0131932746938546
1107	8	-2.008706585420601
1107	9	-2.222271161888578
1107	10-11	-2.2443763272630335
1107	12-13	-2.2689037261829696
1107	14-15	-2.5537800278608245
1107	16-17	-2.590742537613565
1107	18-19	-2.660850060554303
1107	20-21	-2.634984538746302
1107	22-23	-2.7179051814905293
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2308	78-79	-2.6939859650042024
2308	80-81	-2.6573211632798106
2308	82-83	-2.5257710865218392
2308	84-85	-2.568947295157571
2308	86-87	-2.790365931626983
2308	88-89	-2.8947690531468133
2308	90-91	-2.6478704279931904
2308	92-93	-2.5185349729458544
2308	94-95	-2.4216670102165487
2308	96-97	-2.8744058463909816
2308	98-99	-2.73063761566009
2308	100	-2.6797489198126385
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	1784540.0
3	476177.0
4	250559.0
5	199632.0
6	198985.0
7	219986.0
8	250421.0
9	271044.0
10	284913.0
11	284052.0
12	293806.0
13	342955.0
14	433915.0
15	534170.0
16	593848.0
17	533541.0
18	430283.0
19	350830.0
20	330182.0
21	336815.0
22	367563.0
23	423554.0
24	502907.0
25	606428.0
26	728660.0
27	867731.0
28	1026120.0
29	1207094.0
30	1424727.0
31	1690678.0
32	2031741.0
33	2490981.0
34	3151307.0
35	4153803.0
36	5838002.0
37	7707756.0
38	5655292.0
39	734926.0
40	1493.0
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	21.62367190146156	27.373508943016223	27.993559431993887	23.009259723528334
2	23.89521428057833	27.31460053603505	26.857595928751856	21.932589254634767
3	23.908525231988293	26.13503094595286	26.49538167810982	23.461062143949032
4	24.836297215223997	25.632959514932402	26.275118451931966	23.25562481791163
5	25.128630967169922	25.66492752445102	26.105657669145437	23.100783839233618
6	25.07986693377637	25.603927466734618	25.52355500406372	23.792650595425297
7	25.16122081090556	25.999445776042094	25.67784283628804	23.161490576764304
8	24.705910321740888	25.757271547317846	25.92075554363367	23.616062587307592
9	24.372204445330365	25.49284857965396	26.14022225100917	23.99472472400651
10-11	24.371808022133024	25.603855068478058	26.080093261097804	23.944243648291117
12-13	24.06111065472441	25.74521840770349	26.21520578711175	23.978465150460355
14-15	23.7277855604213	25.95073989861162	26.652351223661107	23.669123317305974
16-17	23.57749047435039	25.852639979160287	26.9450003202122	23.624869226277124
18-19	23.14134824668565	25.86515512913418	27.623543014887552	23.36995360929262
20-21	22.824244738159365	25.637656950212822	28.389020632304458	23.149077679323355
22-23	22.895562895988164	25.555842616962842	28.745549121155907	22.803045365893084
24-25	22.725232538024866	25.46423785149764	29.14616771176331	22.664361898714187
26-27	22.764627419282263	25.575936794476444	29.144616826257558	22.51481895998373
28-29	22.81640973582216	25.613544620426552	29.0848513727536	22.485194270997685
30-31	22.79126592104108	25.502110425500753	29.11267891498021	22.59394473847796
32-33	22.827250546258117	25.62024015389312	28.966725610952064	22.585783688896694
34-35	22.95622625638947	25.59552197728503	28.970418693621898	22.477833072703607
36-37	23.06417244244969	25.594916781066463	28.81629310905902	22.524617667424824
38-39	22.93602501703142	25.762827539181448	28.73469378593191	22.56645365785522
40-41	23.000457959849072	25.870890992903966	28.53768065558987	22.59097039165709
42-43	23.141240513588958	25.879221335453153	28.113105606344984	22.86643254461291
44-45	23.17823093164676	26.168514953746413	27.666986234373276	22.98626788023355
46-47	23.475021241208093	26.269916935745098	27.21141494095497	23.04364688209184
48-49	23.562978102671163	26.301435852682715	26.841693605534655	23.293892439111467
50-51	23.486957875029336	26.443166474836556	26.69806991637837	23.371805733755746
52-53	23.6401678933495	26.49709501214277	26.457357442322728	23.405379652185008
54-55	23.703376592117532	26.511920554489777	26.37245784016722	23.412245013225473
56-57	23.635702095644483	26.586919517786605	26.346132571637824	23.43124581493109
58-59	23.758750082181713	26.647259199627875	26.267064463558803	23.326926254631612
60-61	23.68516103862012	26.6458616438607	26.22450050320663	23.444476814312544
62-63	23.677263570666536	26.743632385645434	26.21411706487101	23.364986978817022
64-65	23.684285108952082	26.852427381891285	26.239199227465214	23.224088281691415
66-67	23.70531798057113	26.876294409611486	26.116658835533368	23.30172877428402
68-69	23.704249023040123	27.0027672418926	26.028184543137172	23.26479919193011
70-71	23.775549007219105	27.096704671159667	26.02340803557225	23.10433828604898
72-73	23.700995539856155	27.09988929603737	25.90216944771624	23.296945716390233
74-75	23.662483177473906	27.389370032564837	25.83130763824507	23.11683915171619
76-77	23.55882868762218	27.482485805154067	25.738011090206403	23.22067441701735
78-79	23.5639942596191	27.575732720324147	25.657748917404437	23.20252410265231
80-81	23.506287180125838	27.72773530293522	25.601141864597864	23.164835652341075
82-83	23.515263387279578	27.776404616892503	25.567156542946208	23.14117545288171
84-85	23.454766216374757	27.87202896326335	25.447121133080252	23.226083687281633
86-87	23.445025690509706	28.04856554029919	25.34695607606289	23.159452693128213
88-89	23.596790111929337	28.111198189750976	25.202138162405273	23.089873535914414
90-91	23.551385799705077	28.120903195952344	25.183899040496183	23.143811963846396
92-93	23.590536274641632	28.309004483459475	25.00440939149377	23.09604985040512
94-95	23.66900666817948	28.339006324475193	24.90942616615147	23.082560841193857
96-97	23.58048403075491	28.515036144426393	24.69050991544045	23.21396990937825
98-99	23.625894047812835	28.60618787124936	24.53427143665191	23.233646644285894
100	23.769383420656315	28.70705178072851	24.16958892778707	23.3539758708281
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1814.0
1	1222.5
2	595.5
3	582.5
4	647.0
5	737.5
6	872.5
7	1027.5
8	1212.5
9	1451.5
10	1786.0
11	2205.0
12	2807.0
13	4126.0
14	7434.5
15	10723.5
16	15057.0
17	17304.0
18	17780.0
19	21804.5
20	28866.0
21	36097.5
22	43442.0
23	53202.5
24	64882.5
25	78169.5
26	93711.0
27	115222.0
28	143027.0
29	171699.0
30	205637.5
31	260338.5
32	341715.0
33	437271.0
34	531629.0
35	655068.0
36	828010.0
37	1061905.0
38	1305184.0
39	1437997.5
40	1514857.5
41	1677726.0
42	1993785.0
43	2285654.5
44	2509917.5
45	2705157.5
46	2736826.0
47	2676129.0
48	2627752.5
49	2633857.5
50	2490083.0
51	2197963.0
52	1890275.5
53	1550631.0
54	1308463.0
55	1169452.0
56	1032001.5
57	875354.0
58	769160.0
59	685477.0
60	583983.0
61	500982.5
62	436791.0
63	378775.5
64	327876.0
65	281003.5
66	238534.0
67	197102.0
68	158066.5
69	126403.0
70	100277.5
71	79939.5
72	64607.5
73	52565.5
74	42801.5
75	31959.5
76	22258.5
77	17162.5
78	14293.0
79	10012.5
80	5423.5
81	2755.0
82	1513.5
83	863.5
84	511.5
85	313.5
86	226.0
87	159.0
88	112.5
89	82.5
90	55.0
91	33.0
92	25.0
93	19.5
94	17.0
95	13.0
96	8.5
97	6.0
98	2.5
99	1.0
100	0.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.30455556916462956
2	0.0024259653623154784
3	0.0
4	3.7950341243959544E-4
5	9.6712159944284E-4
6	0.006810658014641772
7	0.012154310902702529
8	0.014755745584748141
9	0.01576163366180578
10-11	0.01353256119895493
12-13	0.019090449884360615
14-15	0.018074360102667508
16-17	0.02582867579609053
18-19	0.014483360070981013
20-21	0.012705202953018068
22-23	0.014671071436273716
24-25	0.015294395589501115
26-27	0.015245427407250845
28-29	0.015642273717570744
30-31	0.015414775704199697
32-33	0.015909558379020135
34-35	0.01760508168943575
36-37	0.012267549824156277
38-39	0.013062262615259624
40-41	0.014529267741840641
42-43	0.012086979652108407
44-45	0.013617235347429356
46-47	0.010730152935590497
48-49	0.008489858597640627
50-51	0.007435002338332719
52-53	0.007424800633697246
54-55	0.0068065773327875825
56-57	0.009400870821588365
58-59	0.008493939279494815
60-61	0.006568877614781063
62-63	0.00730646085992576
64-65	0.0037623886695624413
66-67	0.008234815981753803
68-69	0.007702286999782112
70-71	0.00862860178068306
72-73	0.009364144684900664
74-75	0.005559929026332782
76-77	0.006757609150537312
78-79	0.0014935295586332466
80-81	0.013583569722132295
82-83	0.017342897880304093
84-85	0.01877929789297869
86-87	0.012769473692221549
88-89	0.0068412631285481905
90-91	0.002108692348152268
92-93	0.004870293792974809
94-95	0.002577970761384026
96-97	0.0023739366686745663
98-99	0.0018261051297496663
100	0.009885451791773333
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	4.9011417E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	26.74395488919947
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.3834203810981	19.893068391764483
2	13.385551666836665	7.1596517988985715
3	4.957510647461167	3.977503233552826
4	2.254598259598586	2.411874965918888
5	1.1684906746548696	1.562503094571004
6	0.709628992087377	1.1386971451471737
7	0.46106300882240575	0.8631453816317497
8	0.3324861109695665	0.7113594842444363
9	0.23173051846066325	0.5577651478946505
>10	1.6471987930824197	9.079981448802203
>50	0.19736716139373178	3.585199845815633
>100	0.16146988118603517	9.886041530951836
>500	0.04651109573033841	8.936137083804239
>1k	0.0625553645945103	29.048528526778817
>5k	2.782960180097644E-4	0.4467109550817667
>10k+	1.3914800542880765E-4	0.7418319651417397
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.0403409270945992E-5	0.0	0.0	2.0403409270945993E-6	0.0
2	5.7129545958648775E-5	0.0	0.0	2.0403409270945993E-6	0.0
3	7.549261430250017E-5	2.0403409270945993E-6	0.0	2.0403409270945993E-6	0.0
4	9.793636450054076E-5	2.0403409270945993E-6	0.0	2.0403409270945993E-6	0.0
5	1.3466250118824354E-4	2.0403409270945993E-6	0.0	2.0403409270945993E-6	0.0
6	1.6118693324047333E-4	2.0403409270945993E-6	0.0	2.0403409270945993E-6	4.0806818541891986E-6
7	1.9383238807398694E-4	2.0403409270945993E-6	0.0	8.161363708378397E-6	4.0806818541891986E-6
8	2.366795475429735E-4	2.0403409270945993E-6	0.0	8.161363708378397E-6	4.0806818541891986E-6
9	2.774863660848655E-4	4.0806818541891986E-6	0.0	8.161363708378397E-6	4.0806818541891986E-6
10-11	3.468579576060819E-4	4.0806818541891986E-6	0.0	9.181534171925696E-6	4.0806818541891986E-6
12-13	4.8764148157560917E-4	1.0201704635472996E-5	0.0	1.0201704635472996E-5	4.0806818541891986E-6
14-15	7.018772789205421E-4	1.3262216026114893E-5	0.0	1.3262216026114893E-5	4.0806818541891986E-6
16-17	0.0010334326795734144	2.0403409270945992E-5	0.0	1.5302556953209494E-5	7.141193244831097E-6
18-19	0.00155269944551899	2.550426158868249E-5	1.0201704635472996E-6	1.6322727416756794E-5	1.4282386489662194E-5
20-21	0.0022494758721217957	2.8564772979324387E-5	2.0403409270945993E-6	1.836306834385139E-5	1.5302556953209494E-5
22-23	0.003319634688382913	2.9584943442871688E-5	2.0403409270945993E-6	2.0403409270945992E-5	1.836306834385139E-5
24-25	0.004636674756822477	3.162528436996629E-5	2.0403409270945993E-6	2.346392066158789E-5	1.836306834385139E-5
26-27	0.006550514546437211	3.672613668770278E-5	2.0403409270945993E-6	2.448409112513519E-5	1.938323880739869E-5
28-29	0.0081225972307636	3.978664807834468E-5	2.0403409270945993E-6	2.448409112513519E-5	2.2443750198040592E-5
30-31	0.00934272110516617	4.0806818541891984E-5	2.0403409270945993E-6	2.8564772979324387E-5	2.8564772979324387E-5
32-33	0.010288419124874517	4.0806818541891984E-5	2.0403409270945993E-6	3.060511390641899E-5	2.8564772979324387E-5
34-35	0.011204532201139992	4.0806818541891984E-5	2.0403409270945993E-6	3.162528436996629E-5	3.060511390641899E-5
36-37	0.012199198403098608	4.4887500396081185E-5	4.0806818541891986E-6	3.3665625297060885E-5	3.264545483351359E-5
38-39	0.01313163420678084	4.4887500396081185E-5	4.0806818541891986E-6	3.978664807834468E-5	3.468579576060819E-5
40-41	0.01409161461297885	4.590767085962848E-5	5.100852317736498E-6	4.386732993253388E-5	3.468579576060819E-5
42-43	0.015381110078902638	4.9988352713817676E-5	6.121022781283797E-6	4.4887500396081185E-5	3.468579576060819E-5
44-45	0.01744185441526818	5.2028693640912284E-5	6.121022781283797E-6	4.692784132317578E-5	3.5705966224155486E-5
46-47	0.02068293597795795	5.304886410445958E-5	6.121022781283797E-6	4.9988352713817676E-5	3.978664807834468E-5
48-49	0.024947248515585663	5.304886410445958E-5	6.121022781283797E-6	5.100852317736498E-5	4.2847159468986584E-5
50-51	0.030644900554497333	5.508920503155418E-5	8.161363708378397E-6	5.406903456800688E-5	4.2847159468986584E-5
52-53	0.03850429380566573	5.508920503155418E-5	1.0201704635472996E-5	5.508920503155418E-5	4.2847159468986584E-5
54-55	0.04914161122907342	5.7129545958648775E-5	1.0201704635472996E-5	6.121022781283798E-5	4.2847159468986584E-5
56-57	0.06334136391118828	5.7129545958648775E-5	1.0201704635472996E-5	6.733125059412177E-5	4.386732993253388E-5
58-59	0.0816381211749091	6.019005734929067E-5	1.0201704635472996E-5	6.733125059412177E-5	4.4887500396081185E-5
60-61	0.10510204183649699	6.121022781283798E-5	1.0201704635472996E-5	6.733125059412177E-5	4.692784132317578E-5
62-63	0.13510933585127727	6.529090966702718E-5	1.0201704635472996E-5	7.345227337540557E-5	4.896818225027038E-5
64-65	0.17297092226490818	6.529090966702718E-5	1.0201704635472996E-5	8.059346662023668E-5	5.100852317736498E-5
66-67	0.2208852684263342	6.631108013057448E-5	1.2242045562567595E-5	8.569431893797316E-5	5.2028693640912284E-5
68-69	0.28009902264201014	6.937159152121638E-5	1.4282386489662194E-5	8.875483032861507E-5	5.304886410445958E-5
70-71	0.3529738795350479	6.937159152121638E-5	1.4282386489662194E-5	9.181534171925696E-5	5.304886410445958E-5
72-73	0.44153895815744315	7.039176198476367E-5	1.4282386489662194E-5	9.385568264635156E-5	5.7129545958648775E-5
74-75	0.5476040409115288	7.141193244831097E-5	1.4282386489662194E-5	9.487585310989886E-5	6.325056873993257E-5
76-77	0.6752467491401034	7.243210291185828E-5	1.4282386489662194E-5	9.895653496408806E-5	6.529090966702718E-5
78-79	0.8254291035902921	7.447244383895287E-5	1.4282386489662194E-5	1.0303721681827725E-4	6.733125059412177E-5
80-81	1.0007790633761926	7.753295522959478E-5	1.4282386489662194E-5	1.0405738728182455E-4	6.937159152121638E-5
82-83	1.2035012168695305	8.161363708378397E-5	1.4282386489662194E-5	1.0405738728182455E-4	7.243210291185828E-5
84-85	1.4366979024499535	8.263380754733126E-5	1.6322727416756794E-5	1.0711789867246646E-4	7.345227337540557E-5
86-87	1.7029501513902363	8.569431893797317E-5	1.6322727416756794E-5	1.1017841006310836E-4	7.549261430250017E-5
88	1.9246331931190646	8.773465986506776E-5	1.6322727416756794E-5	1.1017841006310836E-4	7.549261430250017E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATCTCGA	32270	0.0	49.6375	1
ATACGGC	6350	0.0	42.261112	5
TGATACG	6660	0.0	40.29347	3
TCTCGAG	42115	0.0	37.830517	2
CTCGAGG	48725	0.0	32.20604	3
ACGACGC	4990	0.0	26.842642	40-41
TCGAGGG	58755	0.0	26.548204	4
GATCCGG	49930	0.0	24.962345	1
ATGATAC	11505	0.0	24.34664	2
TATCTCG	19020	0.0	24.265005	2
ATATCTC	19720	0.0	23.85663	1
TACGGCG	10820	0.0	23.67277	6
CGATCTA	4450	0.0	21.808172	52-53
CGACGCT	6220	0.0	21.2699	42-43
ATCCGGC	58580	0.0	21.140276	2
GATACGG	13065	0.0	20.827728	4
ACGGCGA	13480	0.0	18.863056	7
ACGCTCT	7180	0.0	18.851309	44-45
AGGGCGC	88130	0.0	17.609945	7
CGGCGAC	15055	0.0	16.82723	8
>>END_MODULE