FastQCFastQC Report
Wed 25 May 2016
SRR491030_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR491030_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34769139
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA379410.10912263314889678No Hit
GATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT366980.10554762371308647No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCTCGA231850.042.9482351
TCTCGAG268850.036.6594962
CTCGAGG347550.028.317753
GATCCGG371300.028.162141
TCGAGGG407700.024.139894
ATCCGGC473750.022.063972
TCCGGCG508250.020.5385863
TATCTCG152300.020.3059862
CGAGGGC547950.017.978195
GCGCCGT578050.017.9507949
GGATCCG251850.017.654172
ATATCTC186900.017.2805231
CGGCGCG639200.016.235215
CGTCGAC553750.015.84069612-13
CCGGCGC661250.015.7934774
GCCGTCG572850.015.41499510-11
AGGGCGC640300.015.2517897
ATATGGA246250.015.2187451
TATGGAT242650.015.050082
TCGACTT592800.014.86065314-15
Produced by FastQC (version 0.11.5)