##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR491030_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	34769139
Sequences flagged as poor quality	0
Sequence length	100
%GC	45
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.697290059440355	34.0	31.0	34.0	30.0	34.0
2	31.945780567071274	34.0	31.0	34.0	30.0	34.0
3	31.99948710262857	34.0	31.0	34.0	30.0	34.0
4	35.35695031159673	37.0	35.0	37.0	33.0	37.0
5	35.13838611304122	37.0	35.0	37.0	32.0	37.0
6	35.04627425487873	37.0	35.0	37.0	32.0	37.0
7	34.96211456372273	37.0	35.0	37.0	32.0	37.0
8	34.908245010036055	37.0	35.0	37.0	32.0	37.0
9	36.44046558069787	39.0	37.0	39.0	32.0	39.0
10-11	36.303236858985784	39.0	37.0	39.0	32.0	39.0
12-13	36.165443254145586	39.0	37.0	39.0	32.0	39.0
14-15	37.468355946346556	40.0	38.0	41.0	32.0	41.0
16-17	37.34337725475457	40.0	38.0	41.0	31.5	41.0
18-19	37.20686823737568	40.0	38.0	41.0	31.0	41.0
20-21	37.0852418433485	40.0	38.0	41.0	31.0	41.0
22-23	36.89747604909054	40.0	38.0	41.0	30.0	41.0
24-25	36.67675574307434	40.0	37.5	41.0	30.0	41.0
26-27	36.318096085151836	40.0	37.0	41.0	29.0	41.0
28-29	36.01626551638222	40.0	36.0	41.0	27.5	41.0
30-31	35.711644038697656	40.0	36.0	41.0	27.0	41.0
32-33	35.49617350317476	40.0	35.0	41.0	25.5	41.0
34-35	35.279135672585966	39.0	35.0	41.0	25.0	41.0
36-37	34.95992184333354	39.0	35.0	41.0	24.0	41.0
38-39	34.670292223802264	39.0	35.0	40.0	22.5	41.0
40-41	34.21263812428602	39.0	34.5	40.0	17.0	41.0
42-43	33.76276004418746	38.0	34.0	40.0	11.5	41.0
44-45	33.27599738664797	38.0	33.0	40.0	5.0	41.0
46-47	32.81820582327333	38.0	33.0	40.0	2.0	41.0
48-49	32.281032728477975	38.0	32.0	40.0	2.0	41.0
50-51	31.835471364418886	38.0	31.0	40.0	2.0	41.0
52-53	31.33372513193381	37.0	30.5	40.0	2.0	41.0
54-55	30.869046325823597	37.0	30.0	40.0	2.0	41.0
56-57	30.338984781877976	36.0	28.5	39.0	2.0	40.0
58-59	29.80113463551686	36.0	28.0	39.0	2.0	40.0
60-61	29.220609374307486	35.0	26.5	39.0	2.0	40.0
62-63	28.606955582075244	34.5	26.0	38.0	2.0	40.0
64-65	27.990825139500863	34.0	24.5	38.0	2.0	40.0
66-67	27.385716755885156	34.0	22.5	38.0	2.0	40.0
68-69	26.62542673259755	33.5	19.5	37.0	2.0	39.0
70-71	25.90099376633974	32.5	15.5	36.0	2.0	39.0
72-73	25.20712961859654	32.0	8.0	36.0	2.0	38.5
74-75	24.401586662816126	31.0	4.5	35.0	2.0	38.0
76-77	23.51681258198542	30.0	2.0	34.0	2.0	36.0
78-79	23.515395750812235	31.0	2.0	34.0	2.0	36.0
80-81	23.02739309995568	30.0	2.0	34.0	2.0	36.0
82-83	22.297573776560874	29.5	2.0	34.0	2.0	36.0
84-85	21.516142346809335	29.0	2.0	34.0	2.0	35.0
86-87	20.806539802438017	29.0	2.0	34.0	2.0	35.0
88-89	20.121708449553495	27.0	2.0	33.5	2.0	35.0
90-91	19.337375639356498	26.0	2.0	33.0	2.0	35.0
92-93	18.580891462397155	25.0	2.0	32.5	2.0	34.0
94-95	17.604304768662807	22.5	2.0	32.0	2.0	34.0
96-97	16.626148709060644	18.5	2.0	31.5	2.0	34.0
98-99	15.726355058720321	2.0	2.0	31.0	2.0	34.0
100	14.901260569035086	2.0	2.0	31.0	2.0	34.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.11108954397425563
1101	2	0.10477060483930956
1101	3	0.1452621961787699
1101	4	0.1849185303920109
1101	5	0.21786660718515094
1101	6	0.245828565968651
1101	7	0.28132594305584036
1101	8	0.28803781656846894
1101	9	0.3419328359254479
1101	10-11	0.31096865228757054
1101	12-13	0.32701044412054614
1101	14-15	0.35759691148815165
1101	16-17	0.3343535931097392
1101	18-19	0.37032186781417664
1101	20-21	0.3455782921746504
1101	22-23	0.3820645425464235
1101	24-25	0.37074675682222136
1101	26-27	0.373714960258404
1101	28-29	0.36322395513926153
1101	30-31	0.3615400333589278
1101	32-33	0.3335081543671379
1101	34-35	0.29471270535472627
1101	36-37	0.2732490116416173
1101	38-39	0.27063300007001345
1101	40-41	0.24977626354600346
1101	42-43	0.23889717478345318
1101	44-45	0.1365409211655404
1101	46-47	0.07188878697255774
1101	48-49	0.09773540598891373
1101	50-51	-0.047884288466462976
1101	52-53	-0.07340902801092852
1101	54-55	-0.07761076842250247
1101	56-57	-0.07218455772367349
1101	58-59	-0.03174485294042739
1101	60-61	0.01962213223305298
1101	62-63	-0.06299902050288608
1101	64-65	-0.10269448040862628
1101	66-67	-0.2547177341393301
1101	68-69	-0.2718433401444855
1101	70-71	-0.1494437304969054
1101	72-73	-0.21462880894264913
1101	74-75	-0.2040538652241608
1101	76-77	-0.2708202198827365
1101	78-79	-0.26444174518015373
1101	80-81	-0.21021538273677365
1101	82-83	-0.26564428867824574
1101	84-85	-0.2115056658365333
1101	86-87	-0.260210837634272
1101	88-89	-0.17375100933769616
1101	90-91	-0.2992761249636793
1101	92-93	-0.328210851094191
1101	94-95	-0.4563442438346179
1101	96-97	-0.46325803071384897
1101	98-99	-0.47196350542828647
1101	100	-0.3337627995694241
1102	1	0.1721285587077972
1102	2	0.17123590209786244
1102	3	0.16476762445297766
1102	4	0.16139144233945757
1102	5	0.1932561438452609
1102	6	0.20881351170561402
1102	7	0.23073773704758338
1102	8	0.17236137315470756
1102	9	0.21443622549382724
1102	10-11	0.1528065797628244
1102	12-13	0.189830383818979
1102	14-15	0.2171614572751963
1102	16-17	0.24961828277358222
1102	18-19	0.2648661112143387
1102	20-21	0.2519759667207566
1102	22-23	0.2588210032529972
1102	24-25	0.3227491163190166
1102	26-27	0.3140959972808801
1102	28-29	0.23492626097528557
1102	30-31	0.27672231360175914
1102	32-33	0.23651932012072763
1102	34-35	0.19969738335964138
1102	36-37	0.24259725430288626
1102	38-39	0.21122512907259505
1102	40-41	0.29449341626403225
1102	42-43	0.27200416979828645
1102	44-45	0.2514927685225601
1102	46-47	0.13613903073687794
1102	48-49	0.1758217160876825
1102	50-51	0.09918847452669155
1102	52-53	0.1299522747715116
1102	54-55	0.05014355643172763
1102	56-57	0.07027106177359599
1102	58-59	0.12766892702573074
1102	60-61	0.21441965096540372
1102	62-63	0.16323254847233315
1102	64-65	0.10738876152007037
1102	66-67	0.12064347353545202
1102	68-69	0.1265581482458451
1102	70-71	0.22457206961527731
1102	72-73	0.1786548166449755
1102	74-75	0.12947494827562878
1102	76-77	0.03046819261931688
1102	78-79	0.02296974171704136
1102	80-81	0.10385371451302561
1102	82-83	0.11515072359260614
1102	84-85	0.15823721228940002
1102	86-87	0.12375204629994485
1102	88-89	0.24706125796975442
1102	90-91	0.17523574549037235
1102	92-93	0.16842785083624534
1102	94-95	0.15807883273891932
1102	96-97	0.15619770015775813
1102	98-99	0.20941650098479236
1102	100	0.33319858262363056
1103	1	0.08074256128339741
1103	2	0.07364107716480106
1103	3	0.0712479452545054
1103	4	0.07830302206888717
1103	5	0.08204189309904564
1103	6	0.1048407941368481
1103	7	0.13582893790767514
1103	8	0.09926164394373416
1103	9	0.11157893803796526
1103	10-11	0.0976681395057426
1103	12-13	0.11987079753686203
1103	14-15	0.1397578665144934
1103	16-17	0.14035323865468285
1103	18-19	0.16147535214452802
1103	20-21	0.18644442322452903
1103	22-23	0.1438040694391347
1103	24-25	0.1602540475803167
1103	26-27	0.1901442248038876
1103	28-29	0.2013733676281504
1103	30-31	0.16666986520024096
1103	32-33	0.2039775870057099
1103	34-35	0.12791028823465211
1103	36-37	0.14024566210874667
1103	38-39	0.17161636423936244
1103	40-41	0.16715500608192713
1103	42-43	0.04360057668999673
1103	44-45	0.08234332919127496
1103	46-47	0.011429965597123726
1103	48-49	0.02274529851312934
1103	50-51	-0.01072098894950102
1103	52-53	-0.03204255668857314
1103	54-55	-0.0019020706065973059
1103	56-57	0.06804498458957653
1103	58-59	0.14267444458596756
1103	60-61	0.09640980883464678
1103	62-63	0.013666277878993327
1103	64-65	0.18466194589614915
1103	66-67	0.12078183005435861
1103	68-69	0.1497277869743776
1103	70-71	0.33820400605468137
1103	72-73	0.19421388016722219
1103	74-75	0.22991764396735448
1103	76-77	0.0123226274821846
1103	78-79	0.08025047890248871
1103	80-81	0.10419382418769274
1103	82-83	0.17815507527025076
1103	84-85	0.2362630227517215
1103	86-87	0.3289639671934985
1103	88-89	0.45645952672716916
1103	90-91	0.4290624357221908
1103	92-93	0.3862633008081815
1103	94-95	0.309570780948075
1103	96-97	0.4473308295613201
1103	98-99	0.429594572227721
1103	100	0.4814988863624823
1104	1	-0.009059022271035388
1104	2	-0.010032817325676291
1104	3	-0.027749555316049168
1104	4	-0.021482738183507877
1104	5	-0.03167042479628179
1104	6	-0.04760132598467237
1104	7	-0.05782825999566654
1104	8	-0.03994630244933717
1104	9	-0.08304704945073382
1104	10-11	-0.06589771716572557
1104	12-13	-0.07372920140223016
1104	14-15	-0.020020630601706557
1104	16-17	-0.05579352174127905
1104	18-19	-0.03182770378611366
1104	20-21	-0.021638338515003852
1104	22-23	-0.03405014120947669
1104	24-25	-0.027054007969226745
1104	26-27	0.045065815143502164
1104	28-29	0.0474987532386848
1104	30-31	0.08918317923464514
1104	32-33	0.058496357994464176
1104	34-35	0.05390094215412233
1104	36-37	0.05566928004493121
1104	38-39	0.08743390664129436
1104	40-41	0.18500151835722534
1104	42-43	0.12682656568453154
1104	44-45	0.13663501051060223
1104	46-47	0.06741752239152277
1104	48-49	0.13104031984730113
1104	50-51	0.07705631541422164
1104	52-53	0.12906830049556106
1104	54-55	0.12863491965589802
1104	56-57	0.19470960103472734
1104	58-59	0.1675143649581301
1104	60-61	0.18981590626490075
1104	62-63	0.22162225718039963
1104	64-65	0.2656813931007811
1104	66-67	0.18138120323553153
1104	68-69	0.22632667090548964
1104	70-71	0.35789192625926347
1104	72-73	0.3512905474091639
1104	74-75	0.31753098894005305
1104	76-77	0.31901098756163293
1104	78-79	0.3219722134804677
1104	80-81	0.2965786129607153
1104	82-83	0.2557639200349797
1104	84-85	0.27880372332462855
1104	86-87	0.3752671126784257
1104	88-89	0.559671203762445
1104	90-91	0.5268695175807885
1104	92-93	0.5149105074417335
1104	94-95	0.6443667468616106
1104	96-97	0.7361003259575547
1104	98-99	0.6708831404403952
1104	100	0.6289059647805608
1105	1	-0.11075657536226657
1105	2	-0.13918997537421873
1105	3	-0.1394181907533607
1105	4	-0.08093222457626581
1105	5	-0.0887184421492151
1105	6	-0.10866836079031827
1105	7	-0.07700576508536727
1105	8	-0.1304236137524981
1105	9	-0.181046676170304
1105	10-11	-0.23569848087138467
1105	12-13	-0.13480366972854796
1105	14-15	-0.12879904186568325
1105	16-17	-0.1224373581373257
1105	18-19	-0.051764984462955965
1105	20-21	-0.065216043327041
1105	22-23	-0.05823914776349426
1105	24-25	-0.10666421177838004
1105	26-27	-0.006166694108642901
1105	28-29	-0.03164440009973646
1105	30-31	-0.007663584719345806
1105	32-33	-0.0024455259776701155
1105	34-35	-0.029676794954880847
1105	36-37	-0.007608863899044138
1105	38-39	-0.04725375323478431
1105	40-41	0.10547004557757589
1105	42-43	0.0630953919185373
1105	44-45	0.04859448104531339
1105	46-47	0.015752599958183566
1105	48-49	0.01923283475179005
1105	50-51	-0.03680068928473901
1105	52-53	-0.050886262586242736
1105	54-55	-0.03236427536154096
1105	56-57	0.018690653558273596
1105	58-59	0.05043325131805432
1105	60-61	0.11479312743233194
1105	62-63	0.05373995325930281
1105	64-65	0.14033806645388935
1105	66-67	0.11369134764781208
1105	68-69	0.11598907995739083
1105	70-71	0.2058023362222272
1105	72-73	0.1679237076261728
1105	74-75	0.08443014570205065
1105	76-77	0.10071931653087063
1105	78-79	0.16497330757131579
1105	80-81	0.22437312160990075
1105	82-83	0.16919325582526312
1105	84-85	0.10163279066668807
1105	86-87	0.27716583864944866
1105	88-89	0.43610095371638735
1105	90-91	0.48498947132560843
1105	92-93	0.5800291923733099
1105	94-95	0.5709783836257252
1105	96-97	0.6972891553528981
1105	98-99	0.6484946974043524
1105	100	0.6579474540096744
1106	1	-0.1966996663210523
1106	2	-0.18385055827809182
1106	3	-0.1662964671393432
1106	4	-0.15927723981931052
1106	5	-0.17355030586478648
1106	6	-0.16962745499536425
1106	7	-0.16008196657925566
1106	8	-0.2103926580772324
1106	9	-0.21436795659771235
1106	10-11	-0.26558544180559807
1106	12-13	-0.26814947980855663
1106	14-15	-0.34883247996646816
1106	16-17	-0.3131308009335072
1106	18-19	-0.3394740082149994
1106	20-21	-0.298485999600544
1106	22-23	-0.32943988271526337
1106	24-25	-0.4294226898215925
1106	26-27	-0.3841210579566763
1106	28-29	-0.3763640629354299
1106	30-31	-0.5255881322713591
1106	32-33	-0.5261941740172844
1106	34-35	-0.5579772660116191
1106	36-37	-0.3013485523389434
1106	38-39	-0.4567186539992889
1106	40-41	-0.4391581292905755
1106	42-43	-0.3834180858144407
1106	44-45	-0.4422311080389605
1106	46-47	-0.3721773155849917
1106	48-49	-0.3272981945040243
1106	50-51	-0.38076944139201174
1106	52-53	-0.4496014148428813
1106	54-55	-0.3757021616445648
1106	56-57	-0.44539351431058805
1106	58-59	-0.42580313699345496
1106	60-61	-0.2480739610009941
1106	62-63	-0.46572210431268957
1106	64-65	-0.3773694334215101
1106	66-67	-0.3706183542473376
1106	68-69	-0.3439635480783103
1106	70-71	-0.22237518142963353
1106	72-73	-0.33907945720357446
1106	74-75	-0.30976219283912343
1106	76-77	-0.3446317170034874
1106	78-79	-0.3061455478632027
1106	80-81	-0.10461951597218544
1106	82-83	-0.18948280904980663
1106	84-85	-0.232276992807396
1106	86-87	-0.14030822710806845
1106	88-89	0.05836539529265039
1106	90-91	-0.0713191060629228
1106	92-93	0.039766257151612194
1106	94-95	-0.06627084660130933
1106	96-97	-0.004321176086367728
1106	98-99	-0.07237110166368588
1106	100	-0.11747680433568242
1107	1	-0.23254939883601367
1107	2	-0.22351089150459202
1107	3	-0.18978877619000656
1107	4	-0.23200347905662255
1107	5	-0.2255928294854641
1107	6	-0.233141289546694
1107	7	-0.2098404302149035
1107	8	-0.23551824234634466
1107	9	-0.30381957199497833
1107	10-11	-0.2623079555681116
1107	12-13	-0.2574329876412378
1107	14-15	-0.30079239662688195
1107	16-17	-0.31000533523743456
1107	18-19	-0.3883294957430863
1107	20-21	-0.27439163632101327
1107	22-23	-0.27308431707798064
1107	24-25	-0.34708889783989605
1107	26-27	-0.18560596660459794
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2207	96-97	0.39682304136729485
2207	98-99	0.1963284757990298
2207	100	0.20794159924143862
2208	1	-0.304055311236862
2208	2	-0.2960154607377419
2208	3	-0.5226416883628886
2208	4	-0.3843311249419301
2208	5	-0.36277842429940677
2208	6	-0.3423636966467072
2208	7	-0.3422076719396969
2208	8	-0.3102824328789424
2208	9	-0.36089011823877115
2208	10-11	-0.4377084432531859
2208	12-13	-0.4291343221308779
2208	14-15	-0.6273771792247018
2208	16-17	-0.6576937156318792
2208	18-19	-0.6248523414925273
2208	20-21	-0.7115179147787387
2208	22-23	-0.7227925759609448
2208	24-25	-0.6357217188333735
2208	26-27	-0.6951264732077576
2208	28-29	-0.9744519418458566
2208	30-31	-0.8249779291503714
2208	32-33	-0.7174495655838342
2208	34-35	-0.6176370906657667
2208	36-37	-0.6420705801050062
2208	38-39	-0.5281872201798592
2208	40-41	-0.9724798324803601
2208	42-43	-1.1270751058076556
2208	44-45	-0.9764616484885806
2208	46-47	-0.8589300806434075
2208	48-49	-0.847341195943585
2208	50-51	-0.6775193501016332
2208	52-53	-0.486877107290546
2208	54-55	-0.3993817346825459
2208	56-57	-0.8889276574251568
2208	58-59	-0.6898863152793488
2208	60-61	-0.989501148401235
2208	62-63	-0.6287527111008728
2208	64-65	-0.7479910335244675
2208	66-67	-0.5862140491519376
2208	68-69	-0.8630622124684741
2208	70-71	-1.0405491557192157
2208	72-73	-0.9773703102571005
2208	74-75	-0.8486881740513184
2208	76-77	-0.9175558325962996
2208	78-79	-1.1512298258272438
2208	80-81	-1.3187866131716177
2208	82-83	-1.6696024264416813
2208	84-85	-1.905032053456349
2208	86-87	-1.9206334699759147
2208	88-89	-2.4799666452891422
2208	90-91	-2.216076676807237
2208	92-93	-2.3900661477543714
2208	94-95	-2.6992552630723594
2208	96-97	-2.9977696141130448
2208	98-99	-3.3732925500918327
2208	100	-3.399419044783375
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	536593.0
3	218796.0
4	202703.0
5	169933.0
6	155440.0
7	159851.0
8	164564.0
9	183816.0
10	211326.0
11	237036.0
12	262555.0
13	305287.0
14	368866.0
15	469818.0
16	565080.0
17	570804.0
18	525345.0
19	496643.0
20	501004.0
21	520556.0
22	545152.0
23	576714.0
24	606547.0
25	648268.0
26	698157.0
27	761688.0
28	850312.0
29	968668.0
30	1118256.0
31	1298457.0
32	1538896.0
33	1888126.0
34	2452095.0
35	3394063.0
36	4541130.0
37	4382905.0
38	1611702.0
39	61974.0
40	13.0
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	20.422307429937547	28.680747575979876	28.203231031928905	22.693713962153673
2	23.44744115419847	28.63561481386378	27.47639407632051	20.440549955617243
3	22.743842687705495	26.822187294025124	27.393073397024548	23.04089662124483
4	23.3808155936992	26.468735342847122	27.17206296290789	22.97838610054579
5	23.843893529860598	26.708569019325658	27.020931070517694	22.42660638029605
6	23.91485224256368	26.553323347650544	26.28186689720845	23.24995751257733
7	24.028721293173774	26.92817099691483	26.42285170417201	22.620256005739382
8	23.283573958942757	26.672430364434568	26.914392923945506	23.129602752677165
9	23.364172347783736	26.314437839839165	27.035339145798865	23.286050666578234
10-11	23.294935405796373	26.617383200191984	26.748275420931666	23.339405973079977
12-13	23.069075570665564	26.68905672226992	26.90755879223125	23.33430891483326
14-15	22.791265535265115	26.915532901658562	27.368022194598957	22.92517936847737
16-17	22.60323959923903	26.862810835544355	27.509816347488236	23.024133217728377
18-19	22.22540693685494	26.794505723476746	28.18844817573926	22.791639163929048
20-21	21.962973040643924	26.543675944654005	28.980433794935383	22.512917219766692
22-23	21.911328714611788	26.530325891377977	29.3366622910761	22.22168310293414
24-25	21.879231053458557	26.35819161861304	29.710652063876147	22.05192526405225
26-27	21.93692634254656	26.400381202165928	29.75547365138481	21.907218803902705
28-29	21.970559427875298	26.472798330957815	29.701877310063974	21.854764931102917
30-31	21.93691953951052	26.433304666336415	29.650322413476303	21.979453380676755
32-33	21.96898677187078	26.462107232052357	29.61269539841868	21.95621059765818
34-35	22.031481456341997	26.547901497012738	29.553547967590415	21.867069079054847
36-37	22.041356414129375	26.489096771093735	29.472344532636864	21.997202282140027
38-39	22.08903714117936	26.536447666848467	29.39196763383943	21.982547558132744
40-41	22.16908234826153	26.690527599758724	29.139002458452186	22.001387593527564
42-43	22.322395709786928	26.777356576038024	28.70340816768663	22.196839546488416
44-45	22.437512556162087	26.9740717140389	28.299083502630307	22.28933222716871
46-47	22.580216053382294	27.181146346059343	27.873316978303702	22.365320622254657
48-49	22.703390792575483	27.1810733931341	27.550806981832466	22.564728832457956
50-51	22.799941642566182	27.23819417824917	27.356214825822544	22.6056493533621
52-53	22.858114259983328	27.317479684524255	27.228199332224438	22.59620672326798
54-55	22.89946696872084	27.261466327481287	27.149813851421527	22.68925285237634
56-57	22.882323643107796	27.299295282558706	27.1487534241938	22.6696276501397
58-59	22.914272046905346	27.370547013890224	27.100087043265887	22.615093895938546
60-61	22.891282356759874	27.352606767742543	26.992248213404146	22.763862662093437
62-63	22.914443755124957	27.415673169375005	26.961071760459653	22.708811315040386
64-65	22.97387371095612	27.5193813389008	26.825782773161443	22.68096217698164
66-67	22.997827151464413	27.5214125610857	26.727534095552496	22.753226191897387
68-69	23.009315630243865	27.605168635151994	26.622818994168725	22.76269674043542
70-71	23.068907186250083	27.688125751099786	26.52142568544179	22.721541377208343
72-73	23.06514732221804	27.748593987819852	26.355335399918122	22.830923290043984
74-75	23.1304995912388	27.747768208137757	26.335381266786094	22.786350933837348
76-77	22.67533301598111	28.17746755026553	26.11702174551095	23.030177688242414
78-79	22.86259770870349	28.12561217365419	26.017416614596105	22.994373503046216
80-81	22.82296342310195	28.224986181688738	25.93517904035787	23.016871354851443
82-83	22.795957283133475	28.354383781410363	25.81126254770707	23.038396387749096
84-85	22.773137882220198	28.339112275699463	25.663576460894767	23.224173381185572
86-87	22.685571698032188	28.47441041330052	25.58874397976713	23.251273908900156
88-89	22.654309308051086	28.565192189080257	25.48203968005304	23.29845882281561
90-91	22.603892393098285	28.5793601741451	25.35005929410782	23.466688138648795
92-93	22.586707285758344	28.702034931066983	25.229989930796968	23.4812678523777
94-95	22.552827950521472	28.791337114766556	25.03596369951807	23.6198712351939
96-97	22.450845846482835	28.848410590931284	24.916552878078313	23.784190684507568
98-99	22.45536746711565	28.88044574339531	24.779362487035925	23.88482430245311
100	23.010532345542202	29.01191971547124	24.15716327660905	23.820384662377506
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	4533.0
1	3248.0
2	1735.0
3	1508.5
4	1560.5
5	1797.0
6	2167.0
7	2632.0
8	3196.5
9	3999.5
10	5043.0
11	6255.0
12	7898.5
13	10501.5
14	16247.5
15	22850.5
16	31772.5
17	37437.5
18	39712.0
19	47514.0
20	65487.5
21	81331.0
22	94338.5
23	117601.5
24	144258.5
25	175518.0
26	216292.0
27	267902.5
28	326082.5
29	383642.5
30	438612.5
31	498770.0
32	569034.0
33	645148.5
34	720870.0
35	803211.0
36	896701.5
37	999488.5
38	1093179.0
39	1159011.5
40	1211483.5
41	1253566.0
42	1285691.0
43	1314131.0
44	1321437.5
45	1309920.5
46	1290443.5
47	1249593.0
48	1197405.5
49	1145281.5
50	1090360.0
51	1038069.5
52	982606.0
53	918937.0
54	858405.0
55	798914.0
56	734027.0
57	670467.5
58	616482.0
59	567847.0
60	514513.0
61	462845.0
62	416763.5
63	373025.0
64	331003.5
65	291424.5
66	254136.0
67	218188.5
68	185473.5
69	157103.5
70	132312.0
71	111315.5
72	93987.5
73	79532.5
74	66946.0
75	55369.0
76	45779.0
77	37986.0
78	31229.0
79	25510.0
80	20614.0
81	16364.0
82	12786.0
83	9798.5
84	7234.0
85	5159.5
86	3601.5
87	2519.5
88	1801.0
89	1270.5
90	899.0
91	625.5
92	418.0
93	271.5
94	191.0
95	120.5
96	67.0
97	40.0
98	17.5
99	5.0
100	1.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.16948363317250967
2	1.725668271509398E-5
3	9.203564114716789E-5
4	0.004480985278352737
5	0.008835421550128118
6	7.477895843207392E-5
7	6.643822845311182E-4
8	0.0027323080965565467
9	8.68586363326397E-4
10-11	2.1570853393867474E-4
12-13	3.796470197320676E-4
14-15	4.012178731259351E-4
16-17	0.002109629461920239
18-19	0.0018133897419777925
20-21	9.706884027240365E-4
22-23	0.0014351807791386493
24-25	4.8318711602263144E-4
26-27	5.363952210608379E-4
28-29	6.198025208504588E-4
30-31	0.0010167062232976202
32-33	0.0037317576371390733
34-35	4.6880654709338643E-4
36-37	0.0033319778209060626
38-39	0.0013805346172075185
40-41	8.959094442919624E-4
42-43	6.802009103532877E-4
44-45	0.0010181442801905447
46-47	0.0023972408405051387
48-49	0.0032557608055810644
50-51	0.0018594075625513763
52-53	0.0016422609717197771
54-55	0.0015574156150372316
56-57	0.0021168197463848617
58-59	6.413733742443263E-4
60-61	0.0016494512561843998
62-63	0.0015789864684310994
64-65	0.009640733410165837
66-67	0.006052781462319213
68-69	0.0032241235539367252
70-71	0.0075354181189243715
72-73	0.00144812329117497
74-75	0.005453111737969698
76-77	0.0035965802892041704
78-79	0.0017659338645112839
80-81	0.005366828324394228
82-83	0.0035462482979518126
84-85	0.006478446302624864
86-87	0.0021800942496735397
88-89	0.0023397185647881587
90-91	0.00388419166778907
92-93	0.006432428482051281
94-95	0.0011490074574466741
96-97	0.0020535452430961837
98-99	0.0040912718603701975
100	0.003436955974089551
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	3.4769139E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	46.019275492218256
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.20609755626363	33.688915711519016
2	13.283485963943143	12.225928001434275
3	5.24427321977781	7.240129621722524
4	2.521706683159032	4.641884582514537
5	1.3816249257098319	3.179068904157816
6	0.8714207810565285	2.4061291795850632
7	0.5882523021639986	1.894966132655167
8	0.4230066629405147	1.5573168125522765
9	0.32214621569184776	1.3342441903828837
>10	1.8891913380531797	16.930548684462046
>50	0.18530545681598035	5.767415716694294
>100	0.0797041083233658	7.098527328068703
>500	0.0034244801804849557	0.9593226634244998
>1k	2.4136550628391882E-4	0.20075029903086433
>5k	4.382015267417079E-5	0.1663585209839
>10k+	7.512026171711719E-5	0.7084936508121853
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	37941	0.10912263314889678	No Hit
GATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	36698	0.10554762371308647	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	2.8761137858489966E-6	0.0	0.0	0.0
3	0.0	1.4380568929244984E-5	0.0	5.752227571697993E-6	0.0
4	0.0	1.4380568929244984E-5	0.0	8.62834135754699E-6	0.0
5	0.0	1.725668271509398E-5	0.0	8.62834135754699E-6	0.0
6	0.0	1.725668271509398E-5	0.0	1.1504455143395986E-5	0.0
7	0.0	1.725668271509398E-5	0.0	1.1504455143395986E-5	0.0
8	2.8761137858489966E-6	2.0132796500942977E-5	0.0	1.4380568929244984E-5	2.8761137858489966E-6
9	1.1504455143395986E-5	2.3008910286791973E-5	0.0	2.0132796500942977E-5	2.8761137858489966E-6
10-11	3.738947921603696E-5	2.7323080965565465E-5	0.0	2.3008910286791973E-5	2.8761137858489966E-6
12-13	1.4092957550660085E-4	3.451336543018796E-5	0.0	2.876113785848997E-5	2.8761137858489966E-6
14-15	4.6449237641461296E-4	4.601782057358395E-5	2.8761137858489966E-6	2.876113785848997E-5	2.8761137858489966E-6
16-17	0.0010181442801905447	5.464616193113094E-5	2.8761137858489966E-6	3.3075308537263464E-5	2.8761137858489966E-6
18-19	0.0019859565691287325	6.327450328867792E-5	2.8761137858489966E-6	3.738947921603696E-5	2.8761137858489966E-6
20-21	0.00348441185155606	8.19692428966964E-5	2.8761137858489966E-6	4.026559300188595E-5	2.8761137858489966E-6
22-23	0.00518563315588574	9.63498118259414E-5	2.8761137858489966E-6	4.026559300188595E-5	2.8761137858489966E-6
24-25	0.006973137873790893	1.0354009629056388E-4	2.8761137858489966E-6	4.457976368065945E-5	5.752227571697993E-6
26-27	0.008848364062164438	1.0785426696933737E-4	2.8761137858489966E-6	5.177004814528194E-5	5.752227571697993E-6
28-29	0.01100976357222996	1.2079677900565786E-4	2.8761137858489966E-6	5.752227571697994E-5	7.190284464622492E-6
30-31	0.013339415738767648	1.3230123414905386E-4	2.8761137858489966E-6	5.896033260990443E-5	8.62834135754699E-6
32-33	0.015400151266328453	1.3949151861367633E-4	2.8761137858489966E-6	6.039838950282893E-5	8.62834135754699E-6
34-35	0.01732427138906143	1.4955791686414784E-4	2.8761137858489966E-6	6.039838950282893E-5	8.62834135754699E-6
36-37	0.0191362230741463	1.5818625822169483E-4	2.8761137858489966E-6	6.039838950282893E-5	8.62834135754699E-6
38-39	0.021090542391630693	1.668145995792418E-4	2.8761137858489966E-6	6.471256018160243E-5	8.62834135754699E-6
40-41	0.023145525691619803	1.725668271509398E-4	2.8761137858489966E-6	6.902673086037592E-5	8.62834135754699E-6
42-43	0.02530836325857825	1.725668271509398E-4	2.8761137858489966E-6	7.765507221792291E-5	1.1504455143395986E-5
44-45	0.027603502059685744	1.768809978297133E-4	2.8761137858489966E-6	8.34072997896209E-5	1.1504455143395986E-5
46-47	0.030009371241548434	1.826332254014113E-4	2.8761137858489966E-6	8.62834135754699E-5	1.1504455143395986E-5
48-49	0.03294875953068611	1.869473960801848E-4	5.752227571697993E-6	8.772147046839441E-5	1.1504455143395986E-5
50-51	0.03645330417874311	1.8838545297310928E-4	5.752227571697993E-6	9.059758425424339E-5	1.1504455143395986E-5
52-53	0.04074158983344396	1.941376805448073E-4	5.752227571697993E-6	9.34736980400924E-5	1.1504455143395986E-5
54-55	0.04568419137442546	2.0995630636697676E-4	7.190284464622492E-6	9.49117549330169E-5	1.4380568929244984E-5
56-57	0.0514507995150527	2.3152715976084424E-4	8.62834135754699E-6	9.49117549330169E-5	1.4380568929244984E-5
58-59	0.058337653975268126	2.4159355801131572E-4	1.0066398250471488E-5	1.0066398250471488E-4	1.4380568929244984E-5
60-61	0.06706378320153397	2.516599562617872E-4	1.1504455143395986E-5	1.0354009629056388E-4	1.4380568929244984E-5
62-63	0.07764356776277952	2.5741218383348516E-4	1.1504455143395986E-5	1.0354009629056388E-4	1.869473960801848E-5
64-65	0.09039338017544812	2.617263545122587E-4	1.4380568929244984E-5	1.0354009629056388E-4	2.0132796500942977E-5
66-67	0.10577339864527563	2.674785820839567E-4	1.4380568929244984E-5	1.0354009629056388E-4	2.0132796500942977E-5
68-69	0.1243502175880743	2.804210941202772E-4	1.869473960801848E-5	1.0785426696933737E-4	2.0132796500942977E-5
70-71	0.14667173668004835	2.8761137858489966E-4	2.0132796500942977E-5	1.1216843764811087E-4	2.0132796500942977E-5
72-73	0.1734354135142662	2.8761137858489966E-4	2.0132796500942977E-5	1.1216843764811087E-4	2.0132796500942977E-5
74-75	0.20571691464663533	3.0055389062122017E-4	2.0132796500942977E-5	1.1216843764811087E-4	2.0132796500942977E-5
76-77	0.24415329928072133	3.091822319787671E-4	2.0132796500942977E-5	1.1792066521980887E-4	2.1570853393867473E-5
78-79	0.2890940152415048	3.1349640265754063E-4	2.0132796500942977E-5	1.2654900657735584E-4	2.3008910286791973E-5
80-81	0.3411487986515858	3.1781057333631416E-4	2.0132796500942977E-5	1.2942512036320485E-4	2.3008910286791973E-5
82-83	0.4020390036117949	3.235628009080121E-4	2.0132796500942977E-5	1.3373929104197835E-4	2.3008910286791973E-5
84-85	0.47271949989903406	3.307530853726346E-4	2.4446967179716473E-5	1.4092957550660085E-4	2.3008910286791973E-5
86-87	0.553288075382022	3.379433698372571E-4	2.876113785848997E-5	1.4955791686414784E-4	2.3008910286791973E-5
88	0.6206624788724276	3.451336543018796E-4	3.163725164433896E-5	1.5243403064999682E-4	2.3008910286791973E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATCTCGA	23185	0.0	42.948235	1
TCTCGAG	26885	0.0	36.659496	2
CTCGAGG	34755	0.0	28.31775	3
GATCCGG	37130	0.0	28.16214	1
TCGAGGG	40770	0.0	24.13989	4
ATCCGGC	47375	0.0	22.06397	2
TCCGGCG	50825	0.0	20.538586	3
TATCTCG	15230	0.0	20.305986	2
CGAGGGC	54795	0.0	17.97819	5
GCGCCGT	57805	0.0	17.950794	9
GGATCCG	25185	0.0	17.65417	2
ATATCTC	18690	0.0	17.280523	1
CGGCGCG	63920	0.0	16.23521	5
CGTCGAC	55375	0.0	15.840696	12-13
CCGGCGC	66125	0.0	15.793477	4
GCCGTCG	57285	0.0	15.414995	10-11
AGGGCGC	64030	0.0	15.251789	7
ATATGGA	24625	0.0	15.218745	1
TATGGAT	24265	0.0	15.05008	2
TCGACTT	59280	0.0	14.860653	14-15
>>END_MODULE