FastQCFastQC Report
Wed 25 May 2016
SRR491029_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR491029_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34008734
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCTCGA219200.042.9746321
TCTCGAG258350.036.2398222
GATCCGG372500.027.4118861
CTCGAGG348550.026.7537693
TCGAGGG424450.021.9474894
ATCCGGC474950.021.5434742
TCCGGCG510950.020.0165333
GCGCCGT585800.017.4085489
GGATCCG251900.017.31512
ATATGGA204700.017.2484111
ATATCTC177500.016.8149871
TATGGAT210850.016.2502142
CGGCGCG642450.015.8532055
CGTCGAC556650.015.09986212-13
CGAGGGC629500.015.0444685
GCCGTCG577400.014.69143110-11
TCGACTT582750.014.45581214-15
TATCTCG200750.014.2114492
AGGGCGC662500.013.9960517
CCGGCGC778950.013.2504464
Produced by FastQC (version 0.11.5)