FastQCFastQC Report
Wed 25 May 2016
SRR491028_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR491028_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22586541
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT257350.11393953593868136No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCTCGA134500.045.4676061
TCTCGAG161850.037.6659242
CTCGAGG207450.029.2770393
GATCCGG227550.027.4542331
TCGAGGG254950.023.8224034
TATCTCG83600.023.3888242
ATCCGGC281350.022.1856692
ATATCTC104050.019.1839921
GGATCCG149550.018.1347142
GCGCCGT355550.017.371119
TCCGGCG365200.017.2742753
CGGCGCG385700.016.0636375
CGAGGGC396050.015.5251515
CGTCGAC345000.015.31315712-13
ATGGATC162700.015.0752811
AGGGCGC407800.014.7871827
TCGACTT360350.014.70151414-15
TGGATCC192400.014.6322581
GCCGTCG368750.014.4542210-11
CCGGCGC467300.013.5503314
Produced by FastQC (version 0.11.5)