##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR491028_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	22586541
Sequences flagged as poor quality	0
Sequence length	100
%GC	45
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.67480005017147	33.0	31.0	34.0	30.0	34.0
2	31.93091766463931	34.0	31.0	34.0	30.0	34.0
3	32.001125803194036	34.0	31.0	34.0	30.0	34.0
4	35.372149812580865	37.0	35.0	37.0	33.0	37.0
5	35.149657975517364	37.0	35.0	37.0	32.0	37.0
6	35.08638817249618	37.0	35.0	37.0	32.0	37.0
7	35.012291434974486	37.0	35.0	37.0	32.0	37.0
8	34.92933765289692	37.0	35.0	37.0	32.0	37.0
9	36.46344652773526	39.0	37.0	39.0	32.0	39.0
10-11	36.29990134390211	39.0	37.0	39.0	32.0	39.0
12-13	36.158294933252506	39.0	37.0	39.0	32.0	39.0
14-15	37.44949421427566	40.0	38.0	41.0	32.0	41.0
16-17	37.334162765338874	40.0	38.0	41.0	32.0	41.0
18-19	37.1857172375354	40.0	38.0	41.0	31.0	41.0
20-21	37.03690954715022	40.0	38.0	41.0	31.0	41.0
22-23	36.840125630569105	40.0	38.0	41.0	30.0	41.0
24-25	36.62817566886404	40.0	37.0	41.0	30.0	41.0
26-27	36.267561952049235	40.0	37.0	41.0	28.5	41.0
28-29	35.945307318194494	40.0	36.0	41.0	27.5	41.0
30-31	35.64680264676207	39.5	35.5	41.0	26.5	41.0
32-33	35.42691689710257	39.0	35.0	41.0	25.5	41.0
34-35	35.216484542719485	39.0	35.0	41.0	25.0	41.0
36-37	34.9042796769988	39.0	35.0	40.5	23.5	41.0
38-39	34.56412745980006	39.0	35.0	40.0	21.5	41.0
40-41	34.10402708409402	38.5	34.0	40.0	16.5	41.0
42-43	33.7005870664304	38.0	33.5	40.0	11.5	41.0
44-45	33.230262039681065	38.0	33.0	40.0	5.0	41.0
46-47	32.67844804124722	38.0	32.5	40.0	2.0	41.0
48-49	32.13810412581546	38.0	32.0	40.0	2.0	41.0
50-51	31.658545591376743	37.5	31.0	40.0	2.0	41.0
52-53	31.091320711745993	37.0	30.0	40.0	2.0	41.0
54-55	30.553036252872893	36.0	29.0	39.0	2.0	40.0
56-57	30.02022106882147	36.0	28.0	39.0	2.0	40.0
58-59	29.460497116402195	35.0	27.5	39.0	2.0	40.0
60-61	28.860629699784486	35.0	26.0	38.0	2.0	40.0
62-63	28.205482304705267	34.0	25.5	38.0	2.0	40.0
64-65	27.539495578362352	34.0	22.5	37.5	2.0	40.0
66-67	26.88816346425068	33.5	21.0	37.0	2.0	39.0
68-69	26.137296144637638	32.5	18.5	36.0	2.0	39.0
70-71	25.349078063790287	32.0	10.5	35.5	2.0	38.5
72-73	24.58733302722183	31.0	7.0	35.0	2.0	38.0
74-75	23.76746784733439	30.5	2.0	34.5	2.0	37.0
76-77	22.808763170066634	29.5	2.0	34.0	2.0	36.0
78-79	22.782887029935218	30.0	2.0	34.0	2.0	36.0
80-81	22.336293193366792	29.5	2.0	34.0	2.0	35.5
82-83	21.764960690528042	29.0	2.0	34.0	2.0	35.0
84-85	20.970091856916028	29.0	2.0	34.0	2.0	35.0
86-87	20.240436306736832	27.0	2.0	33.0	2.0	35.0
88-89	19.58429832173063	26.5	2.0	33.0	2.0	35.0
90-91	18.729115139852535	25.0	2.0	32.0	2.0	34.0
92-93	17.895576795047987	23.5	2.0	32.0	2.0	34.0
94-95	16.91460224033419	19.0	2.0	31.5	2.0	34.0
96-97	15.884775141089554	8.5	2.0	31.0	2.0	34.0
98-99	14.901351937864234	2.0	2.0	31.0	2.0	34.0
100	14.019047848008245	2.0	2.0	30.0	2.0	34.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.18757196390795272
1101	2	0.22305826927843952
1101	3	0.17942116839816435
1101	4	0.18940053605766138
1101	5	0.20779937285268346
1101	6	0.23216823820055765
1101	7	0.25316814158097145
1101	8	0.2602363570415065
1101	9	0.307032419995636
1101	10-11	0.31617468573987395
1101	12-13	0.31714993778936673
1101	14-15	0.3313253780712557
1101	16-17	0.3599918486187974
1101	18-19	0.3777627470254217
1101	20-21	0.3329294044868334
1101	22-23	0.30214293368482714
1101	24-25	0.32415759408480227
1101	26-27	0.32560189322316546
1101	28-29	0.33117145337032383
1101	30-31	0.3136149278656717
1101	32-33	0.2971632388054033
1101	34-35	0.28155977381554465
1101	36-37	0.23822872467492573
1101	38-39	0.19134142652084307
1101	40-41	0.2409715507023691
1101	42-43	0.08801561530677304
1101	44-45	0.020205442022621867
1101	46-47	0.03384097255616325
1101	48-49	0.054430965604261417
1101	50-51	-0.036592899856824346
1101	52-53	-0.19377851251615041
1101	54-55	-0.24299094408862842
1101	56-57	-0.26226507027048385
1101	58-59	-0.36046972225000573
1101	60-61	-0.5091534738763208
1101	62-63	-0.5592858942809151
1101	64-65	-0.49298932051311084
1101	66-67	-0.5014812208269923
1101	68-69	-0.623939211970324
1101	70-71	-0.625285130296561
1101	72-73	-0.7660960476335994
1101	74-75	-0.7725452918062743
1101	76-77	-0.6243788015429459
1101	78-79	-0.6906331643669823
1101	80-81	-0.8273046911341169
1101	82-83	-0.8610901866953746
1101	84-85	-0.893603448573014
1101	86-87	-1.0402423204671258
1101	88-89	-0.903625882504258
1101	90-91	-0.8171850980284532
1101	92-93	-0.7829354234769816
1101	94-95	-0.8374066791867811
1101	96-97	-0.7733477672067472
1101	98-99	-0.8004923106562511
1101	100	-0.8878119134046507
1102	1	0.14899115778733218
1102	2	0.1677457599333394
1102	3	0.1739369137222866
1102	4	0.14414880754997483
1102	5	0.18648521691727638
1102	6	0.16082114598613373
1102	7	0.18410835397571645
1102	8	0.16316166667256482
1102	9	0.17372579394089627
1102	10-11	0.15994324414619854
1102	12-13	0.19064100397145012
1102	14-15	0.2106569947713126
1102	16-17	0.2081405782988881
1102	18-19	0.27601421367865697
1102	20-21	0.21410897040271237
1102	22-23	0.24509326245975416
1102	24-25	0.2789788212402726
1102	26-27	0.24583985967615263
1102	28-29	0.18514965523416294
1102	30-31	0.1651000851981479
1102	32-33	0.18301201908000309
1102	34-35	0.1164148259129476
1102	36-37	0.16529345300956777
1102	38-39	0.1381081216264306
1102	40-41	0.17758788781242174
1102	42-43	0.060121703607819654
1102	44-45	0.0021082162931023163
1102	46-47	0.07617395935942284
1102	48-49	0.1265978465638824
1102	50-51	0.0424348126561398
1102	52-53	0.05453549600632357
1102	54-55	0.022082810939831887
1102	56-57	0.019447783651546757
1102	58-59	-0.07886903746038953
1102	60-61	-0.15534782485558551
1102	62-63	-0.1603535254106916
1102	64-65	-0.1817401259487461
1102	66-67	-0.18466030705978653
1102	68-69	-0.19295008158138316
1102	70-71	-0.256376736475147
1102	72-73	-0.2651664659668356
1102	74-75	-0.16625751284950496
1102	76-77	-0.15727614664690748
1102	78-79	-0.16610894727214287
1102	80-81	-0.2569216096849871
1102	82-83	-0.11768613264166561
1102	84-85	-0.2059347895207857
1102	86-87	-0.2561282276183192
1102	88-89	-0.1527186731620489
1102	90-91	-0.01035349506649652
1102	92-93	-0.018583661141644114
1102	94-95	-0.06444209506377518
1102	96-97	-0.09117972873293212
1102	98-99	-0.018323831285499637
1102	100	-0.010896431877466028
1103	1	0.0774168123759651
1103	2	0.10939209193123389
1103	3	0.10496294324677535
1103	4	0.08994720147307333
1103	5	0.13662710283776391
1103	6	0.13595864055866969
1103	7	0.1328189761604719
1103	8	0.09917761246165213
1103	9	0.1360348065729866
1103	10-11	0.13380358783972213
1103	12-13	0.1398358461803184
1103	14-15	0.15126882439405165
1103	16-17	0.19279365542191584
1103	18-19	0.22594952008885372
1103	20-21	0.19349998924529643
1103	22-23	0.18393724364748465
1103	24-25	0.15389763358641062
1103	26-27	0.24369454388777
1103	28-29	0.2661720057747985
1103	30-31	0.260069654019226
1103	32-33	0.13070575351098768
1103	34-35	0.11214088156145152
1103	36-37	0.1383078233059578
1103	38-39	0.1789303358854042
1103	40-41	0.2263486317858323
1103	42-43	0.11849072586247189
1103	44-45	0.04961043140882282
1103	46-47	0.1484504245583338
1103	48-49	-0.0024646082590606966
1103	50-51	0.12032071359518781
1103	52-53	0.03194560832969273
1103	54-55	-0.017872585391845774
1103	56-57	0.05898995242099048
1103	58-59	0.05068849810982101
1103	60-61	-0.11149258881757262
1103	62-63	0.05148200397815117
1103	64-65	0.0923461868500901
1103	66-67	0.048587088971878245
1103	68-69	0.023657462340384683
1103	70-71	0.049696744382941915
1103	72-73	-0.03859769891718656
1103	74-75	-0.03955544646746745
1103	76-77	-0.045660394298145945
1103	78-79	-0.04550627365871307
1103	80-81	-0.05282964946526647
1103	82-83	0.016766104788857206
1103	84-85	-0.169295605270392
1103	86-87	-0.11451837164941736
1103	88-89	0.1313573143743305
1103	90-91	0.30237253583917223
1103	92-93	0.3719939708246933
1103	94-95	0.20721264486936875
1103	96-97	0.20163118709324124
1103	98-99	0.18207820192254687
1103	100	0.2632137086335824
1104	1	-0.017678243996925858
1104	2	0.006203538461910796
1104	3	-0.00489991622490038
1104	4	-0.0015555184628155416
1104	5	6.564506691475458E-4
1104	6	-0.006770825010228521
1104	7	-0.0202357921927927
1104	8	-0.02303967823034725
1104	9	-0.05957393082720586
1104	10-11	-0.03482158964136062
1104	12-13	0.0356591675448783
1104	14-15	0.03797384859502273
1104	16-17	0.09701146195171617
1104	18-19	0.058996952266674896
1104	20-21	0.07025985588659012
1104	22-23	0.08129041974847695
1104	24-25	0.11565893117507642
1104	26-27	0.15038873134846398
1104	28-29	0.008279161933963053
1104	30-31	0.03703618790267882
1104	32-33	0.04219021644971832
1104	34-35	0.028273299068210633
1104	36-37	0.08306351815840429
1104	38-39	0.10136206351157995
1104	40-41	0.1832505960072126
1104	42-43	0.15013620970201202
1104	44-45	0.1048433167093279
1104	46-47	0.17443766086246626
1104	48-49	0.17998174578215753
1104	50-51	0.1381486684441917
1104	52-53	0.08150139908228482
1104	54-55	0.09130957363322878
1104	56-57	0.14907285111823043
1104	58-59	0.1404635981544473
1104	60-61	0.10244004818348884
1104	62-63	0.08979116824971811
1104	64-65	0.12108718785194483
1104	66-67	0.09098327922927396
1104	68-69	0.05289392025391848
1104	70-71	0.09888541187420685
1104	72-73	0.07146583897560888
1104	74-75	0.2537702432070077
1104	76-77	0.215968018676147
1104	78-79	0.14180459371401
1104	80-81	0.1385751555629895
1104	82-83	0.21143574506735163
1104	84-85	0.23440131520134244
1104	86-87	0.19801779182207113
1104	88-89	0.3081239931040649
1104	90-91	0.42863584204759775
1104	92-93	0.36982025054240353
1104	94-95	0.31652237230114366
1104	96-97	0.46982699193073785
1104	98-99	0.4971908764762567
1104	100	0.588658962249978
1105	1	-0.05611253194925325
1105	2	-0.059469365284648745
1105	3	-0.11785185650887797
1105	4	-0.06983091898197813
1105	5	-0.09397484273900858
1105	6	-0.08888530502585468
1105	7	-0.11706994461338383
1105	8	-0.03615298862354166
1105	9	-0.08767607714132453
1105	10-11	-0.10710540421975878
1105	12-13	-0.09627487886133679
1105	14-15	-0.06057689714950243
1105	16-17	-0.0938477331901737
1105	18-19	-0.03566530985461469
1105	20-21	-0.030411136142184603
1105	22-23	-0.06098933869326828
1105	24-25	-0.08672283959920435
1105	26-27	-0.21576089144244293
1105	28-29	-0.17661994756519306
1105	30-31	-0.014061839271469978
1105	32-33	-0.03580389874270651
1105	34-35	-0.07164526114696201
1105	36-37	-0.03283738460215346
1105	38-39	0.004566722332903339
1105	40-41	0.06157859128326493
1105	42-43	0.031928983529738275
1105	44-45	-0.025907563704556935
1105	46-47	0.09291644274170352
1105	48-49	0.05909577591487647
1105	50-51	0.044607955184794434
1105	52-53	0.05310347962958417
1105	54-55	0.062382182767791505
1105	56-57	0.12764650473866013
1105	58-59	0.1352820720295469
1105	60-61	0.13520947417390872
1105	62-63	0.14083371729672578
1105	64-65	0.1756551799516899
1105	66-67	0.11936354200598132
1105	68-69	0.13222608275559722
1105	70-71	0.21223380554080506
1105	72-73	0.21300124319309788
1105	74-75	0.3646348217961801
1105	76-77	0.3734541950652286
1105	78-79	0.2947010141085187
1105	80-81	0.23949673778401603
1105	82-83	0.2725301978462724
1105	84-85	0.24634003002242366
1105	86-87	0.3007971237424343
1105	88-89	0.41085174088050636
1105	90-91	0.4344803495614187
1105	92-93	0.32620029120015204
1105	94-95	0.41272752742763785
1105	96-97	0.5205022433631079
1105	98-99	0.451340632127998
1105	100	0.5591995886283971
1106	1	-0.1965057720024177
1106	2	-0.18908444068041774
1106	3	-0.19331931447486994
1106	4	-0.20986586886768777
1106	5	-0.21588214474497391
1106	6	-0.2199080822573336
1106	7	-0.21413054420022348
1106	8	-0.21321522086658717
1106	9	-0.2544210857890761
1106	10-11	-0.25266298725042446
1106	12-13	-0.27434377333491966
1106	14-15	-0.31700052741965834
1106	16-17	-0.40493628758215294
1106	18-19	-0.5416908828338833
1106	20-21	-0.48421062516060687
1106	22-23	-0.49608739367275945
1106	24-25	-0.6422018843758792
1106	26-27	-0.7466246691974163
1106	28-29	-0.5398823643465676
1106	30-31	-0.6528953484777489
1106	32-33	-0.64338651364654
1106	34-35	-0.5233613341694792
1106	36-37	-0.5603898786816757
1106	38-39	-0.4782481532879572
1106	40-41	-0.5777164273461395
1106	42-43	-0.6806244142905484
1106	44-45	-0.6580751704381527
1106	46-47	-0.5880074974158163
1106	48-49	-0.5939628112065023
1106	50-51	-0.6315261674350872
1106	52-53	-0.7372667845599636
1106	54-55	-0.6758771030785304
1106	56-57	-0.5021156804720341
1106	58-59	-0.5609242276004061
1106	60-61	-0.4919640990855356
1106	62-63	-0.5569656680253701
1106	64-65	-0.5554304304566884
1106	66-67	-0.6214506839070104
1106	68-69	-0.607054858283508
1106	70-71	-0.6278442916628322
1106	72-73	-0.6164988723186475
1106	74-75	-0.32150193315738207
1106	76-77	-0.4029798151214621
1106	78-79	-0.5397256794707985
1106	80-81	-0.5651220688042358
1106	82-83	-0.48406650261645723
1106	84-85	-0.5111700287990075
1106	86-87	-0.4429011122689879
1106	88-89	-0.25317554904948025
1106	90-91	-0.3899838890614724
1106	92-93	-0.37390777713465084
1106	94-95	-0.3909546749186852
1106	96-97	-0.3578078168375711
1106	98-99	-0.46492335006865737
1106	100	-0.39507299013454755
1107	1	-0.298089855354263
1107	2	-0.2187312499981715
1107	3	-0.2417412101244203
1107	4	-0.2229051428776998
1107	5	-0.21090005989654514
1107	6	-0.2478906815198627
1107	7	-0.2466158835112111
1107	8	-0.23994680820229775
1107	9	-0.2702809074078587
1107	10-11	-0.25723146999194313
1107	12-13	-0.27692457026059003
1107	14-15	-0.2626018990479224
1107	16-17	-0.26146467836617404
1107	18-19	-0.2680718299957974
1107	20-21	-0.24958153896149327
1107	22-23	-0.3451504355008055
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2207	90-91	0.7640705326273434
2207	92-93	0.9113058612404643
2207	94-95	0.9949225239154558
2207	96-97	0.9763590840115128
2207	98-99	1.008356450204161
2207	100	0.9626753906823016
2208	1	-0.8015432773920494
2208	2	-0.6643944917294924
2208	3	-0.7062694224882584
2208	4	-0.6541968343425566
2208	5	-0.7445524788516309
2208	6	-0.7329717810971772
2208	7	-0.7611264557300004
2208	8	-0.7343524010427274
2208	9	-0.8297260658152794
2208	10-11	-0.9558002874167997
2208	12-13	-0.9841308558521007
2208	14-15	-1.0850920108220237
2208	16-17	-0.9909277743926808
2208	18-19	-1.0146794912195034
2208	20-21	-1.1442318321735172
2208	22-23	-0.9858493888333086
2208	24-25	-1.1004414561449636
2208	26-27	-1.4083331724438537
2208	28-29	-1.3423592813870684
2208	30-31	-1.3566071502771067
2208	32-33	-1.3983291769089234
2208	34-35	-1.0555890998511046
2208	36-37	-1.4483965523305855
2208	38-39	-1.4586515596251033
2208	40-41	-2.0899814598218995
2208	42-43	-1.2499170581463517
2208	44-45	-0.984604839949327
2208	46-47	-1.5385003240475896
2208	48-49	-1.6630096051499166
2208	50-51	-1.6109960998972461
2208	52-53	-0.9265723635827321
2208	54-55	-0.8001295315678583
2208	56-57	-0.711703077608874
2208	58-59	-0.8450433492223759
2208	60-61	-0.726904587807315
2208	62-63	-0.9058575380297675
2208	64-65	-0.91408913602044
2208	66-67	-0.8079699575221753
2208	68-69	-1.188934900814079
2208	70-71	-1.195046367021277
2208	72-73	-0.9060772309130414
2208	74-75	-1.7140191337440385
2208	76-77	-1.2166596788648434
2208	78-79	-1.63033936926562
2208	80-81	-1.6234495207223212
2208	82-83	-1.5870243849461687
2208	84-85	-2.164533821042056
2208	86-87	-1.9934351234722207
2208	88-89	-2.1027327188335363
2208	90-91	-2.1788438247034776
2208	92-93	-2.7749159131366206
2208	94-95	-2.8784509493655612
2208	96-97	-2.8516586027875945
2208	98-99	-2.8890956807799917
2208	100	-2.9338896509675507
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	329297.0
3	140637.0
4	137297.0
5	110920.0
6	102294.0
7	103032.0
8	108690.0
9	119539.0
10	138389.0
11	157094.0
12	173994.0
13	200045.0
14	243630.0
15	307908.0
16	368722.0
17	377101.0
18	355267.0
19	338688.0
20	343053.0
21	358571.0
22	377733.0
23	399984.0
24	423021.0
25	448630.0
26	483430.0
27	527398.0
28	586968.0
29	671801.0
30	777327.0
31	908508.0
32	1080988.0
33	1332228.0
34	1734456.0
35	2364121.0
36	2928448.0
37	2333642.0
38	670284.0
39	23389.0
40	17.0
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	21.521660170608712	28.112612586845614	28.019775650870688	22.34595159167499
2	23.328534457755172	28.319294220394347	27.186508992235687	21.165662329614793
3	22.952515543678878	26.741729050285635	27.173218361608647	23.132537044426833
4	23.90942198971388	26.254948196581484	26.803480505376225	23.032149308328414
5	24.117701214251436	26.598227681698315	26.657783804587186	22.626287299463055
6	24.089946454624574	26.48963255694841	26.08036924596196	23.340051742465057
7	24.146387908370173	26.783687228434488	26.223323494522212	22.846601368673124
8	23.469637281152767	26.65951759245628	26.608910293803483	23.261934832587468
9	23.446085263676775	26.33292577062739	26.764541938308717	23.456447027387114
10-11	23.445579426928113	26.527544885033354	26.621585995660173	23.405289692378357
12-13	23.18502387727571	26.599206111934404	26.767225113252163	23.44854489753772
14-15	22.85065891794659	26.818893593468935	27.233826484018266	23.09662100456621
16-17	22.774030671257545	26.730016909961773	27.361142542895468	23.134809875885214
18-19	22.36452762714211	26.686865027730722	28.013333822715087	22.935273522412082
20-21	22.08586683322797	26.478527136859626	28.764629297263706	22.670976732648697
22-23	22.146791959868995	26.415155743604924	29.08955326743645	22.34849902908963
24-25	22.026236789390772	26.274777661249914	29.51820335493101	22.180782194428307
26-27	22.0702743305949	26.343970384299435	29.51958076715021	22.066174517955453
28-29	22.16051361935511	26.35081162526412	29.495156671241073	21.993518084139698
30-31	22.10964383309336	26.28819173259234	29.46922966575814	22.132934768556154
32-33	22.111315530270392	26.414978134991795	29.348594282271357	22.125112052466456
34-35	22.22775980553674	26.377302713983035	29.384601550354102	22.010335930126125
36-37	22.206733230198502	26.380928892199947	29.290423795336213	22.12191408226534
38-39	22.22608859967628	26.43676624041054	29.175247674386505	22.161897485526673
40-41	22.270026094172525	26.568978701174593	28.986460756311722	22.174534448341156
42-43	22.430677581108302	26.623795334030152	28.57025909106848	22.375267993793063
44-45	22.46852924397797	26.866523262071567	28.15173436988424	22.513213124066226
46-47	22.735207191695835	26.993358897955254	27.75225280648624	22.51918110386267
48-49	22.827452134507443	26.975495225039865	27.440009655605945	22.757042984846745
50-51	22.8531754924681	27.0511788177135	27.245339269183205	22.850306420635196
52-53	23.011536708279703	27.10351204293961	27.06577270540932	22.819178543371372
54-55	23.078980943921692	27.051177355623913	26.975669270024525	22.894172430429872
56-57	23.074115369403565	27.13832428229671	26.913253011941507	22.87430733635822
58-59	23.177484216128097	27.1801659168965	26.86778049832452	22.77456936865088
60-61	23.10004016229801	27.151431344718834	26.81333483929037	22.93519365369278
62-63	23.155628661291498	27.253696163051295	26.737422322388415	22.853252853268792
64-65	23.193571673362197	27.2912112891929	26.677096862664072	22.83812017478083
66-67	23.236524323942124	27.316565336739316	26.541673534822557	22.905236804496
68-69	23.236023249046628	27.42403056011492	26.448164767119366	22.891781423719088
70-71	23.306548327278154	27.490248541185835	26.362404282218222	22.840798849317785
72-73	23.285380413702818	27.520704987103787	26.200226681806534	22.99368791738686
74-75	23.342206864104938	27.598796361722506	26.12829687406397	22.93069990010858
76-77	22.920282806795438	27.945876262585283	25.943733293125444	23.19010763749383
78-79	23.09251505445409	27.91560579172848	25.79384853561843	23.198030618198995
80-81	23.082060464342273	28.000036926249273	25.72925634186747	23.188646267540992
82-83	23.14602478808796	28.11031672290529	25.61292823932745	23.1307302496793
84-85	23.060533238673877	28.153865004680394	25.443890530191464	23.34171122645426
86-87	22.975336318353136	28.314756340551178	25.403599401608762	23.306307939486924
88-89	23.024962630679717	28.385864525792726	25.23826110118192	23.350911742345637
90-91	22.934371172180793	28.357837279546615	25.1255163861765	23.582275162096092
92-93	22.947755932211194	28.483581688827993	25.008468752572117	23.5601936263887
94-95	22.965751690011885	28.516617195704878	24.869284200049236	23.648346914234
96-97	22.85146187603484	28.64053064522956	24.637185333191415	23.870822145544185
98-99	22.81747933899851	28.68501778217735	24.527236300040673	23.97026657878347
100	23.61631104243138	28.68058021580982	23.88897350861424	23.81413523314456
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	621.0
1	492.5
2	317.0
3	279.5
4	315.0
5	371.5
6	469.0
7	585.0
8	725.5
9	970.0
10	1295.0
11	1703.0
12	2251.5
13	3147.5
14	5030.0
15	7363.5
16	10773.0
17	13435.0
18	15184.0
19	18997.5
20	29040.0
21	36537.0
22	40655.0
23	49591.0
24	60684.5
25	74056.5
26	89874.0
27	109742.0
28	132256.5
29	154351.0
30	178068.5
31	208447.0
32	250877.5
33	299669.5
34	348015.0
35	408174.5
36	485814.5
37	581228.0
38	665539.5
39	709521.0
40	749713.5
41	824947.0
42	957435.5
43	1073815.5
44	1131511.5
45	1157590.0
46	1135545.5
47	1079295.0
48	1011522.0
49	961951.0
50	911130.5
51	835434.0
52	747664.5
53	646491.5
54	562524.0
55	498612.0
56	433535.5
57	377122.0
58	333980.0
59	294441.0
60	254150.5
61	220934.5
62	194210.0
63	171060.5
64	150228.5
65	131917.5
66	115181.0
67	99892.0
68	85719.0
69	72573.0
70	61172.5
71	51981.5
72	44415.0
73	38237.0
74	32715.5
75	26819.0
76	21925.5
77	18397.5
78	15588.0
79	13188.0
80	11007.0
81	9024.5
82	7220.0
83	5636.0
84	4125.0
85	2833.0
86	1899.0
87	1302.0
88	922.0
89	637.5
90	435.5
91	286.5
92	190.5
93	124.5
94	72.5
95	48.5
96	28.0
97	14.0
98	6.5
99	2.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.15572105529571792
2	0.0
3	0.0015495953984277628
4	0.006840356830202553
5	0.019834821099875364
6	0.003705746709954393
7	6.198381593711051E-4
8	0.003834141757252693
9	0.015553510384790659
10-11	6.309066979313034E-4
12-13	4.7594715808852713E-4
14-15	0.009589781808555813
16-17	6.66326021323938E-4
18-19	9.740313932974509E-4
20-21	0.011794634689747315
22-23	0.008011408209871533
24-25	0.0027538523937773386
26-27	6.331204056433431E-4
28-29	3.98467388167139E-5
30-31	0.0022181351274637405
32-33	0.005042826168026348
34-35	0.002103022326437678
36-37	0.0011046401483077908
38-39	3.6083435706246475E-4
40-41	0.0012042569953495757
42-43	8.722008385436265E-4
44-45	4.2503188071161494E-4
46-47	0.0016824178611501424
48-49	0.0030903359660073666
50-51	0.003716815248514591
52-53	1.2839504729830035E-4
54-55	0.0068292882916423546
56-57	0.0016027243835167147
58-59	0.007172412987008501
60-61	0.003019497319222098
62-63	3.4533840307818714E-4
64-65	8.699871308315868E-4
66-67	0.0018595144781133155
68-69	0.0010559385786429183
70-71	0.005890676221737539
72-73	0.0011312046408522667
74-75	0.00450710890171275
76-77	0.002618816223342919
78-79	0.009718176855854112
80-81	0.004345508238733854
82-83	2.0808852493172818E-4
84-85	0.0013879947354488674
86-87	0.0013924221508729469
88-89	0.0022137077120396613
90-91	0.0010935716097475927
92-93	0.0025236267917252138
94-95	0.00398910129709547
96-97	0.004006810958791787
98-99	0.015655340939544484
100	0.008150871795730033
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	2.2586541E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	37.429733545403465
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.70123713256702	29.45766335565596
2	11.810216747186846	8.841065319213307
3	3.8749092594164916	4.351104632777279
4	1.713358893631238	2.5652226742505806
5	0.8759722416819617	1.6393703799662798
6	0.5357047630131165	1.2030771923150676
7	0.37337757629147744	0.9782796234692988
8	0.26904551373752666	0.8056241512625448
9	0.19656267751670847	0.662155977997932
>10	1.2763742974065793	8.568875292385732
>50	0.1076519183837286	2.8516434938305073
>100	0.19530952198810383	17.840330988093974
>500	0.06285770989254179	16.138685332168322
>1k	0.007267283834698779	3.3846087080513914
>5k	5.940901992329127E-5	0.19664748324425776
>10k+	9.505443187726602E-5	0.5156453953175355
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	25735	0.11393953593868136	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	4.427415424079322E-6
3	0.0	4.427415424079322E-6	0.0	0.0	4.427415424079322E-6
4	0.0	4.427415424079322E-6	0.0	0.0	4.427415424079322E-6
5	0.0	4.427415424079322E-6	0.0	0.0	4.427415424079322E-6
6	0.0	4.427415424079322E-6	0.0	0.0	4.427415424079322E-6
7	0.0	4.427415424079322E-6	0.0	4.427415424079322E-6	4.427415424079322E-6
8	4.427415424079322E-6	8.854830848158645E-6	0.0	4.427415424079322E-6	4.427415424079322E-6
9	1.770966169631729E-5	8.854830848158645E-6	0.0	4.427415424079322E-6	4.427415424079322E-6
10-11	1.2175392416218136E-4	1.5495953984277628E-5	0.0	4.427415424079322E-6	6.6411231361189835E-6
12-13	3.8075772647082166E-4	2.4350784832436273E-5	0.0	4.427415424079322E-6	8.854830848158645E-6
14-15	0.001051511163218839	2.8778200256515595E-5	0.0	8.854830848158645E-6	8.854830848158645E-6
16-17	0.0023044697282332874	3.541932339263458E-5	0.0	8.854830848158645E-6	8.854830848158645E-6
18-19	0.003951468265990795	3.98467388167139E-5	0.0	8.854830848158645E-6	1.1068538560198306E-5
20-21	0.005963728576234848	3.98467388167139E-5	0.0	8.854830848158645E-6	1.3282246272237967E-5
22-23	0.008416516721174792	4.8701569664872545E-5	4.427415424079322E-6	8.854830848158645E-6	1.3282246272237967E-5
24-25	0.011650743688464736	5.755640051303119E-5	4.427415424079322E-6	8.854830848158645E-6	1.770966169631729E-5
26-27	0.015462748368597033	5.755640051303119E-5	4.427415424079322E-6	8.854830848158645E-6	2.213707712039661E-5
28-29	0.019170708786263466	6.198381593711051E-5	4.427415424079322E-6	8.854830848158645E-6	2.6564492544475934E-5
30-31	0.022159214197517006	7.747976992138814E-5	4.427415424079322E-6	8.854830848158645E-6	2.8778200256515595E-5
32-33	0.02474482480517933	8.633460076954678E-5	4.427415424079322E-6	8.854830848158645E-6	3.541932339263458E-5
34-35	0.027427838552171402	8.854830848158645E-5	4.427415424079322E-6	8.854830848158645E-6	3.541932339263458E-5
36-37	0.030221537684765452	8.854830848158645E-5	4.427415424079322E-6	8.854830848158645E-6	3.541932339263458E-5
38-39	0.03266989841428132	9.740313932974509E-5	4.427415424079322E-6	8.854830848158645E-6	3.541932339263458E-5
40-41	0.03503413825073967	1.0183055475382441E-4	4.427415424079322E-6	8.854830848158645E-6	3.541932339263458E-5
42-43	0.03742272887203047	1.0404426246586407E-4	4.427415424079322E-6	8.854830848158645E-6	3.541932339263458E-5
44-45	0.04011681115758274	1.1068538560198306E-4	4.427415424079322E-6	8.854830848158645E-6	3.98467388167139E-5
46-47	0.04318722375418175	1.1511280102606238E-4	4.427415424079322E-6	8.854830848158645E-6	3.98467388167139E-5
48-49	0.04639709993663926	1.1511280102606238E-4	4.427415424079322E-6	8.854830848158645E-6	3.98467388167139E-5
50-51	0.050160403047106686	1.195402164501417E-4	4.427415424079322E-6	8.854830848158645E-6	4.206044652875356E-5
52-53	0.05439079848481447	1.3060875501034E-4	4.427415424079322E-6	8.854830848158645E-6	5.0915277376912207E-5
54-55	0.05938713679088799	1.43891001282578E-4	4.427415424079322E-6	8.854830848158645E-6	5.312898508895187E-5
56-57	0.06538849839822751	1.4610470899461764E-4	4.427415424079322E-6	1.3282246272237967E-5	5.312898508895187E-5
58-59	0.0725432017235397	1.5274583213073663E-4	4.427415424079322E-6	1.3282246272237967E-5	5.534269280099153E-5
60-61	0.08088002496708105	1.593869552668556E-4	4.427415424079322E-6	1.3282246272237967E-5	5.755640051303119E-5
62-63	0.09088377011778828	1.6824178611501425E-4	4.427415424079322E-6	1.3282246272237967E-5	5.755640051303119E-5
64-65	0.10308351331883886	1.7488290925113322E-4	4.427415424079322E-6	1.3282246272237967E-5	5.755640051303119E-5
66-67	0.11864809224218972	1.8152403238725221E-4	8.854830848158645E-6	1.3282246272237967E-5	5.755640051303119E-5
68-69	0.1371723983765376	1.8595144781133154E-4	8.854830848158645E-6	1.3282246272237967E-5	5.755640051303119E-5
70-71	0.15918993528048409	1.992336940835695E-4	8.854830848158645E-6	1.770966169631729E-5	5.755640051303119E-5
72-73	0.18582748017945733	2.058748172196885E-4	8.854830848158645E-6	1.770966169631729E-5	6.198381593711051E-5
74-75	0.21782883886470267	2.1251594035580747E-4	8.854830848158645E-6	1.992336940835695E-5	7.305235449730882E-5
76-77	0.25570980523312536	2.1472964806784715E-4	8.854830848158645E-6	2.4350784832436273E-5	7.96934776334278E-5
78-79	0.29995075385823794	2.2579818662804544E-4	8.854830848158645E-6	2.6564492544475934E-5	7.96934776334278E-5
80-81	0.3516983853348771	2.3243930976416443E-4	8.854830848158645E-6	3.320561568059492E-5	7.96934776334278E-5
82-83	0.4124823716920621	2.3465301747620408E-4	8.854830848158645E-6	3.541932339263458E-5	7.96934776334278E-5
84-85	0.4827786600878815	2.3465301747620408E-4	8.854830848158645E-6	3.541932339263458E-5	7.96934776334278E-5
86-87	0.5638247131333656	2.3686672518824373E-4	8.854830848158645E-6	3.98467388167139E-5	7.96934776334278E-5
88	0.6311989073492926	2.5236267917252134E-4	8.854830848158645E-6	4.8701569664872545E-5	7.96934776334278E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATCTCGA	13450	0.0	45.467606	1
TCTCGAG	16185	0.0	37.665924	2
CTCGAGG	20745	0.0	29.277039	3
GATCCGG	22755	0.0	27.454233	1
TCGAGGG	25495	0.0	23.822403	4
TATCTCG	8360	0.0	23.388824	2
ATCCGGC	28135	0.0	22.185669	2
ATATCTC	10405	0.0	19.183992	1
GGATCCG	14955	0.0	18.134714	2
GCGCCGT	35555	0.0	17.37111	9
TCCGGCG	36520	0.0	17.274275	3
CGGCGCG	38570	0.0	16.063637	5
CGAGGGC	39605	0.0	15.525151	5
CGTCGAC	34500	0.0	15.313157	12-13
ATGGATC	16270	0.0	15.075281	1
AGGGCGC	40780	0.0	14.787182	7
TCGACTT	36035	0.0	14.701514	14-15
TGGATCC	19240	0.0	14.632258	1
GCCGTCG	36875	0.0	14.45422	10-11
CCGGCGC	46730	0.0	13.550331	4
>>END_MODULE